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1.2012年湖南省水稻集中育秧先进县(20个)衡阳县、华容县、桃源县、醴陵市、双峰县、祁东县、株洲县、岳阳县、沅江市、武冈市、汉寿县、湘潭县、赫山区、祁阳县、湘乡市、望城区、邵东县、宁乡县、安仁县、湘阴县。2.2012年湖南省水稻集中育秧先进乡镇(40个)宁乡县双江口镇、望城区丁字湾街道办事处、浏阳市永安镇、株洲县龙凤乡、醴陵市泗汾镇、湘潭县排头乡、湘乡市泉塘镇、衡阳县西渡镇、衡南县松江镇、衡东县新塘镇、 相似文献
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全国粮食生产先进市、县(区)衡阳市、岳阳市,赫山区、衡阳县、桃源县、沅江市、醴陵市、望城区、岳阳县、安仁县、湘阴县、祁东县、双峰县、衡南县、汨罗县、耒阳市、宜章县、湘乡市、华容县、湘潭县、邵东县、道县。全国粮食生产先进工作者许靖波、吴振文、朱克服、钟武云、戴渐红、杨厚波、王峰、陈安柏、黎作风、熊春来、龙国文。全国种粮大户陈文敏(衡阳县)、刘啸(澧 相似文献
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RNA干涉原理及其应用 总被引：5，自引：0，他引：5
RNA干涉是指外源dsRNA引发生物体内的基因的同源序列降解，从而表现出的基因转录后的沉默现象，它与植物中的共抑制和真菌中的基因压制可能具有相同的作用机制。在这一过程中，需要eIF2c类似蛋白因子、RNA螺旋酶、RNA依赖性RNA多聚酶、核糖核酸酶、ATP和转膜蛋白参与。RNA干涉可以用于功能基因组学研究，也可用于克服转基因生物的基因沉默现象，使外源基因在遗传改良生物中能更好地表达，还用于基因治疗，抑制有害基因的表达等。 相似文献
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RNA干扰（RNA interference,RNAi）是由双链RNA分子在mRNA水平关闭相应序列基因表达或使其沉默的过程。RNA干扰技术作为新兴的基因阻断技术具有明显优势,已经广泛地被应用于基因功能研究、抗肿瘤和抗病毒等热门领域。 相似文献
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Increasingly complex networks of small RNAs act through RNA-interference (RNAi) pathways to regulate gene expression, to mediate antiviral responses, to organize chromosomal domains, and to restrain the spread of selfish genetic elements. Historically, RNAi has been defined as a response to double-stranded RNA. However, some small RNA species may not arise from double-stranded RNA precursors. Yet, like microRNAs and small interfering RNAs, such species guide Argonaute proteins to silencing targets through complementary base-pairing. Silencing can be achieved by corecruitment of accessory factors or through the activity of Argonaute itself, which often has endonucleolytic activity. As a specific and adaptive regulatory system, RNAi is used throughout eukarya, which indicates a long evolutionary history. A likely function of RNAi throughout that history is to protect the genome from both pathogenic and parasitic invaders. 相似文献
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RNA interference in Colorado potato beetle(Leptinotarsa decemlineata): A potential strategy for pest control 
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下载免费PDF全文 Colorado potato beetle(CPB), Leptinotarsa decemlineata, is a notorious destructive pest that mainly feeds on the leaves of potato and several other solanaceous plants. CPB is widely recognized for its adaptation to a remarkable variety of host plants and diverse climates, and its high resistance to insecticides and Bacillus thuringiensis toxins. RNA interference(RNAi) is a sequence-specific, endogenous gene silencing mechanism evoked by small RNA molecules that is used as a robust tool for virus and pest control. RNAi has been extensively tested for CPB management by employing various target genes and delivery methods. This article reviews the screening of RNAi target genes, efficient RNAi delivery systems, and factors affecting RNAi efficiency in CPB, which may help understand the mechanisms of RNAi and its application in CPB control strategy. 相似文献
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随着水稻全基因组测序的完成，探究水稻中许多未知基因的功能成了当前的研究重点之一。RNA干扰技术(RNAi)作为新发展起来的基因功能研究方法正在被广泛采用。以农杆菌介导转化水稻技术也逐渐成熟。简要综述了RNAi作用机制及其在水稻基因功能研究中的应用的最新进展。 相似文献
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