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1.
离子注入甜菊的诱变效应研究   总被引:9,自引:1,他引:8  
用75keV的氮和碳离子的不同剂量注入甜菊干种子,以γ射线作对比,研究其M1生物学效应和M2突变。结果表明,离子术能诱发甜菊染色体结构的变异,染色体畸变细胞率随离子注入剂量的提高呈增加趋势。离子束对甜菊的M1损伤效应低于γ射线,而诱发的M2突变高于γ射线。碳离子诱发的染色体畸变细胞率和M2有益性状突变高于氮离子。离子注入杂交一代丰1×日原和日原×丰2的诱变效应大于济宁种和丰2。  相似文献   

2.
氮离子注入对水稻诱变效应的初步研究   总被引:7,自引:4,他引:7  
用15~30KeV的氮离子注入水稻种子,以^60Coγ射线辐照作对比,研究其M1代生物学效应和M2代突变。结果表明,氮离子能诱发水稻染色体结构变异,染色体畸变细胞率随离子注入剂量的提高而呈增加趋势,但染色体畸变效应低于γ辐射。氮离子注入还能抑制根尖细胞有丝分裂,使过氧化物同工酶酶谱发生变化,离子束诱发幼苗叶绿素突变频率明显高于γ射线,而抽穗期和株高突变频率与γ射线相似。  相似文献   

3.
质子对水稻的辐射生物学效应研究   总被引:2,自引:1,他引:1  
用不同能量、剂量的质子辐照水稻干种子,与γ射线辐照作对比,研究其生物学效应.结果表明,质子辐照可诱发各种可见染色体结构变异及有丝分裂行为异常.随着质子能量和剂量的提高,染色体畸变细胞率呈增加趋势.质子处理的能量效应大于剂量效应,能量效应主要表现在8MeV与4.6MeV的差异.质子对染色体的致畸效应和对有丝分裂的抑制作用与γ射线相似,但质子诱发的微核比例明显高于γ射线,而染色体桥的比例低于γ射线.3个供试品种的辐射敏感性随诱变源和考察指标而变化.  相似文献   

4.
郑企成  张维强 《核农学通报》1995,16(4):151-153,157
首次采用放射性^7Be离子注入玉米种子。结果表明,33MeV和14MeV能量的^7Be离子均能不同份额地穿透种皮,注入到胚体,并诱发根尖染色体畸变,引起PAL活性和丙醛含量的变化:M1代幼苗的形态也出现畸形。  相似文献   

5.
辐射敏化剂对离子注入小麦生物学效应的影响   总被引:1,自引:1,他引:1  
韩榕  杨汉民 《核农学报》2003,17(2):85-89
采用中国科学院兰州近代物理研究所 2 0 0kV离子注入机产生的不同剂量Fe1+ ,对春小麦新品系 81 52 9种子进行注入并用辐射敏化剂后处理 ,研究其注入效应。结果表明 :①离子注入随注入剂量的增加染色体畸变率呈上升趋势 ;②不同辐射敏化剂后处理对萌发力、染色体畸变率影响程度不同 ;③不同辐射敏化剂对同一剂量Fe1+ 离子注入小麦的染色体畸变率影响的总趋势 :EDTA >秋水仙素 >咖啡因。  相似文献   

6.
60Co γ射线辐照毛竹种子的细胞学诱变效应   总被引:2,自引:1,他引:1  
利用6种不同剂量的60Co γ射线辐照毛竹种子,研究γ射线辐照对毛竹种子根尖细胞有丝分裂、染色体畸变及核畸变的影响.结果表明,在一定范围内,剂量低于60Gy的辐照对毛竹根尖细胞有丝分裂有促进和刺激效应;高于90Gy对毛竹根尖细胞有丝分裂有一定的抑制作用.同时,辐照还能诱发染色体畸变和核畸变,出现染色体桥、落后染色体(团)、游离染色体(团)、染色体断片、染色体粘连、微核、小核、双核、核出芽、核耳和核裂等类型.染色体畸变率、核畸变率与剂量间存在显著的正相关,相关系数分别为0.8875、0.9982,线性方程式分别为Y=0.0392X-0.4313,Y=0.0530X-0.0783;染色体畸变率与微核率间也存在着较好的相关性,线性表达式为Y=0.8836X-0.4525,相关系数为0.8345.F=25.22>F0.01.  相似文献   

7.
空间环境对水稻的细胞学效应研究   总被引:7,自引:3,他引:4  
通过第17颗返回式卫星搭载5个水稻品种的干种子,进行空间诱变处理,研究其细胞学效应。结果表明,空间环境对根尖细胞染色体有一定的致畸作用,又能促进根尖细胞的有丝分裂活动经空间飞行的水稻染色体畸变细胞率极显高于地面对照组。与γ射线和质子处理的相比较。空间环境对染色体的致畸应变较低,而有丝分裂指数极显大于质子和γ射线处理。  相似文献   

8.
利用6种不同剂量的60Coγ射线辐照毛竹种子,研究γ射线辐照对毛竹种子根尖细胞有丝分裂、染色体畸变及核畸变的影响。结果表明,在一定范围内,剂量低于60Gy的辐照对毛竹根尖细胞有丝分裂有促进和刺激效应;高于90Gy对毛竹根尖细胞有丝分裂有一定的抑制作用。同时,辐照还能诱发染色体畸变和核畸变,出现染色体桥、落后染色体(团)、游离染色体(团)、染色体断片、染色体粘连、微核、小核、双核、核出芽、核耳和核裂等类型。染色体畸变率、核畸变率与剂量间存在显著的正相关,相关系数分别为0.8875、0.9982,线性方程式分别为Y=0.0392X-0.4313,Y=0.0530X-0.0783;染色体畸变率与微核率间也存在着较好的相关性,线性表达式为Y=0.8836X-0.4525,相关系数为0.8345,F=25.22>F0.01。  相似文献   

9.
高能混合粒子场诱变小麦的细胞学效应研究   总被引:11,自引:11,他引:11  
利用北京正负电子对撞机直线加速器E2束流打靶产生高能混合粒子场,以0、109、145、1952、84和560Gy的剂量处理两个冬小麦品种ZY9和ZH7,并与相同剂量的60Coγ射线相比较,研究其细胞学效应。试验结果表明,混合粒子场辐照小麦种子可抑制根尖细胞有丝分裂,诱发染色体出现单微核、双微核、多微核、环状染色体、落后染色体、游离染色体、染色体断片等多种畸变类型。混合粒子场与γ射线诱发的微核畸变率和染色体畸变率均存在明显的剂量效应,但混合粒子场的损伤效应明显高于γ射线。混合粒子场可诱发高频率的环状染色体和染色体断片,显示出混合粒子场处理小麦具有比γ射线处理更高的相对生物学效应。  相似文献   

10.
氮离子注入水稻生物学效应研究   总被引:3,自引:1,他引:3  
本试验研究了氮离子注入水稻干种子引发的生物学效应,并将其与γ射线、叠氮化钠处理进行了比较。结果表明:氮离子注入后,种胚表层细胞出现刻蚀,细胞之间出现裂缝,种胚内层结构也发生变化。氮离子注入的M1代生理损伤较轻,M2代的叶绿素突变频率低于γ射线和叠氮化钠的处理,抽穗期突变频率高于γ射线和叠氮化钠的处理,株高突变频率与叠氮化钠处理的相近,但比γ射线处理的低。用诱变效率衡量其诱变效果,氮离子注入的叶绿素诱变效率低于γ射线和叠氮化钠的处理,株高的诱变效率则高于γ射线和叠氮化钠的处理,抽穗期诱变效率高于γ射线的处理,但比叠氮化钠处理的略低。  相似文献   

11.
低氮和干旱胁迫对富士和秦冠生长及氮素利用的影响   总被引:2,自引:2,他引:0  
【目的】以富士(Fuji)、 秦冠(Qinguan)嫁接在平邑甜茶(Malus hupehensis Rehd.)上的当年生盆栽苗为试验材料,采用砂培方法,研究了缺氮胁迫和干旱对富士和秦冠生长情况、 光合参数、 植株各部位氮磷钾含量及氮素利用效率的影响,分析比较了低氮干旱条件下富士和秦冠生长及氮素利用的差异,以期为果树生产高效肥水利用提供理论指导。【方法】试验共设四个处理: 正常氮正常水(ZZ)、 低氮正常水(DZ)、 正常氮干旱(ZG)、 低氮干旱(DG)。氮素和水分均设置两个水平,分别为正常氮(6 mmol/L NO-3-N)、 低氮(0.3 mmol/LNO-3-N)、 正常供水(保持盆中砂子相对含水量为饱和含水量的80%~85%)、 干旱处理(保持盆中砂子相对含水量为饱和含水量的60%~65%)。【结果】富士和秦冠的生物量(茎和叶)、 株高茎粗等生长指标以及光合速率、 气孔导度、 蒸腾速率均为正常氮正常水(ZZ)>低氮正常水(DZ)>正常氮干旱(ZG)>低氮干旱(DG),并且相对应处理下秦冠的以上指标均高于富士;正常供水下,缺氮处理使富士、 秦冠的根冠比比正常氮处理均有所增加,富士提高了2.05%,秦冠提高了22.40%。富士和秦冠的氮、 磷、 钾含量均表现出正常氮正常水(ZZ)>低氮正常水(DZ)>正常氮干旱(ZG)>低氮干旱(DG); 氮、 钾元素含量在植株各部位的分布顺序依次是叶>根>茎,磷元素则是根>叶>茎;光合氮素利用效率(PNUE)和氮素利用效率表现为秦冠处理之间差异极显著,富士处理之间差异不显著;秦冠的PNUE和NUE明显高于富士,在低氮正常水(DZ)处理下,秦冠氮肥利用率比富士高42.07%,在低氮干旱(DG)处理下高64.14%;低氮胁迫下富士和秦冠的NUE显著提高,并且秦冠提高的幅度高于富士。【结论】施用氮肥能够显著提高富士与秦冠的干物质量,同等水肥条件下,秦冠生长优于富士;水分亏缺会减少叶片对氮的吸收,干旱条件下适度增施氮肥,可提高果树的抗旱能力;低氮干旱胁迫下秦冠的生长指标、 光合指标及氮素利用效率指标均优于富士,表现出较强的抗低氮干旱胁迫的能力。  相似文献   

12.
Staff members of the Department of Botany of Palacký University in Olomouc and Gene Bank Department – Workplace Olomouc, Research Institute of Crop Production in Prague, Czech Republic, conducted an expedition in seven European countries (Austria, Czech Republic, France, Germany, Italy, the Netherlands, Switzerland) in August/September 1999 to collect wild Lactuca spp. germplasm and study its geographic distribution, ecology and biodiversity. During the mission, more than 600 locations were visited resulting in the collection of 602 seed samples (accessions) of wild Lactuca species and 13 seed samples of related genera (Chondrilla and Mycelis). Lactuca serriola f. serriola, L. serriola f. integrifolia, L. saligna and L. viminea subsp. chondrilliflora were prevalent in southern Europe (Italy, France), however, only L. serriola was common in central and western Europe (Austria, Czech Republic, Germany, Netherlands, Switzerland). The greatest diversity of Lactuca species was found in France, where also the most seed samples (165) were collected. The most characteristic habitats with a high density of Lactuca spp. populations were observed along roads and highways, grassy ditches, ruderal communities, and dust-heaps. Natural infections by powdery mildew (Erysiphe cichoracearum) and downy mildew (Bremia lactucae) on some wild Lactuca spp. were observed. Recent observations concerning the geographic distribution, population structure, habitats, and natural occurrence of diseases of Lactuca spp. are discussed. This assemblage of genetic resources of Lactuca spp. can serve as the basis of future studies of species diversification, spatial population structure, plant microevolution, domestication processes, and genetic variability of host-parasite interactions.  相似文献   

13.
Laser-induced breakdown spectroscopy (LIBS) is a new technique for the analysis of plant material. This study investigates the application of LIBS to pasture-based plant samples. The LIBS measurements were obtained from pelletized pasture samples (100 samples) that had been also analyzed by inductively coupled plasma–optical emission spectroscopy (ICP-OES) following microwave digestion for calibration and comparison purposes. Comparisons for elements sodium (Na), potassium (K), magnesium (Mg), calcium (Ca), manganese (Mn), iron (Fe), copper (Cu), zinc (Zn), boron (B), phosphorus (P), and sulfur (S) showed that LIBS could be used for almost all the standard profile total elements with concentrations down to low mg/kg levels (observed error of Na: 0.024 percent, K: 0.18 percent, Mg: 0.016 percent, Ca: 0.073 percent, P: 0.017 percent, Mn: 31 mg/kg, Fe: 150 mg/kg, Zn: 6.6 mg/kg, and B: 1.1 mg/kg). Elemental analysis at less than mg/kg levels was not possible using LIBS. The elements S and Cu were particularly difficult to analyze with reliability using LIBS at the concentration levels found in the plant samples. Replacing microwave digestion and subsequent ICP analysis with a direct analysis of dried plant samples using LIBS has the potential to improve the productivity and reduce the cost of testing.  相似文献   

14.
We have studied the effect of anaerobically digested (ADMSW) and composted municipal solid waste (CMSW) on mineralization and foodweb dynamics to verify the hypothesis that ADMSW would immobilize N right after addition to soil in contrast to addition with CMSW. Another hypothesis was that the mesofauna (enchytraeids and microarthropods) would stimulate N release from the decomposer community. We measured excretion of -N and urea-N from the mesofauna and hypothesized that enchytraeids would release urea. ADMSW and CMSW were amended to pots with sandy loam and barley. The pots were divided into treatments with or without mesofauna. Mesofauna, plant N and biomass, soil N and ergosterol (fungal biomass) were measured over a 113-day period of four equidistant samplings. Soil respiration, N mineralization and N release by the mesofauna were modelled from concurrent studies. ADMSW- and CMSW-treated soils initially (<20 days) immobilized N. The amendments did not increase plant growth substantially, and this was probably due to N-limitation in the early stages of plant growth. Enchytraeid abundance was about three times higher in ADMSW- than CMSW-treated soils, indicating that ADMSW contained more labile compounds, bacteria, and microfauna. The mesofauna did not affect N-content, but the cumulated -N excreted by the mesofauna was estimated to be substantial and about one-fifth of total plant N in ADMSW. An explanation for this discrepancy might be that in the absence of the mesofauna, other members of the detrivore and microbivore community performed the mesofauna’s function. Neither enchytraeids nor microarthropods excreted urea.  相似文献   

15.
正The Center for Agricultural Resources Research(CARR),the Institute of Genetics and Developmental Biology(IGDB),Chinese Academy of Sciences,invites applicants for several research group leader positions.CARR is one of the research organizations in Chinese Academy of Sciences(CAS).We seek nominations and applications from individuals who have expertise and a record of accomplishment in research areas related to ecology,agro-hydrology,  相似文献   

16.
正The Center for Agricultural Resources Research(CARR),the Institute of Genetics and Developmental Biology(IGDB),Chinese Academy of Sciences,invites applicants for several research group leader positions.CARR is one of the research organizations in Chinese Academy of Sciences(CAS).We seek nominations and applications from individuals who have expertise and a record of accomplishment in research areas related to ecology,agro-hydrology,  相似文献   

17.
正The Center for Agricultural Resources Research(CARR),the Institute of Genetics and Developmental Biology(IGDB),Chinese Academy of Sciences,invites applicants for several research group leader positions.CARR is one of the research organizations in Chinese Academy of Sciences(CAS).We seek nominations and applications from individuals who have expertise and a record of accomplishment in research areas related to ecology,agro-hydrology,  相似文献   

18.
The responses of tallgrass prairie plant communities and ecosystem processes to fire and grazing are well characterized. However, responses of invertebrate consumer groups, and particularly soil-dwelling organisms, to these disturbances are not well known. At Konza Prairie Biological Station, we sampled soil macroinvertebrates in 1994 and 1999 as part of a long-term experiment designed to examine the effects and interactions of annual fire, mowing, and fertilization (N and P) on prairie soil communities and processes. For nearly all taxa, in both years, responses were characterized by significant treatment interactions, but some general patterns were evident. Introduced European earthworms (Aporrectodea spp. and Octolasion spp.) were most abundant in plots where fire was excluded, and the proportion of the total earthworm community consisting of introduced earthworms was greater in unburned, unmowed, and fertilized plots. Nymphs of two Cicada genera were collected (Cicadetta spp. and Tibicen spp.). Cicadetta nymphs were more abundant in burned plots, but mowing reduced their abundance. Tibicen nymphs were collected almost exclusively from unburned plots. Treatment effects on herbivorous beetle larvae (Scarabaeidae, Elateridae, and Curculionidae) were variable, but nutrient additions (N or P) usually resulted in greater densities, whereas mowing usually resulted in lower densities. Our results suggest that departures from historical disturbance regimes (i.e. frequent fire and grazing) may render soils more susceptible to increased numbers of European earthworms, and that interactions between fire, aboveground biomass removal, and vegetation responses affect the structure and composition of invertebrate communities in tallgrass prairie soils.  相似文献   

19.
“Wicked” problems are those that are complex and that change when solutions are applied. Many conflicts in conservation fall into this category. The study approached the problem of how to constrain the apparent wickedness of a problem in the conservation management of a species by using simple empirical indicators to carry out iterative assessment of the risk to a population and to document how this risk evolves in relation to the addition of new data and the implementation of management actions. Effects of high levels of uncertainty within data and also concerning population structure were examined through stochastic simulation and by exploration of scenarios. Historical trends in the example used, the Steller sea lion, showed rapid declines in abundance in some regions during the 1980s. The current total population is 130,000-150,000 Steller sea lions through Alaska and British Columbia and this number has been stable since about 1990 in spite of regional differences in population dynamics. Regional differences in the sequence of changes in the number of pups and non-pups, suggested that an internal re-distribution of juveniles could have happened between 1980 and 1990. Current productivity also appears close to the long-term mean. Stochastic population projection using various scenarios showed that, based upon this history, the risk of extinction for the population has declined and is below reasonable thresholds for considering the population to be endangered. The trends in risk suggest that management actions taken since 1990 have probably been effective. Consequently, the conservation management objectives for the Steller sea lion are probably being met. The approach provides a mechanism, based upon experience and scenario analysis, for exploring future policy options and may help to constrain the debate amongst stakeholders about the cost-benefit trade-offs associated with different options.  相似文献   

20.
Nutrient distributions under no tillage (NT) compared with conventional disk-and-bed tillage (CT) management in the warm, humid region of the southeastern USA need to be assessed so that future placement, quantity, and type of fertilizers can be altered, if necessary, to efficiently match crop demands. We determined soil-profile distributions of pH, N, P, S, K, Ca, Mg, Na, Zn, Fe, Mn, and Cu to a depth of 0.9 m at the end of 8.5 years of continuous CT and NT management on a Weswood silty clay loam (fine, mixed, thermic Fluventic Ustochrept) in southcentral Texas. Most dramatic changes occurred within the 0–0.05 m depth, where soil under NT had lower pH, Fe, and Cu than under CT, but greater P, K, Zn, and Mn. Greater P and K under NT than under CT also occurred below the till-zone (0.15–0.3 m). At a depth of 0–0.3 m, soil under NT contained greater amounts of extractable P, K, Zn, Fe, Mn, and Cu than under CT. Nitrogen fertilization had little effect on nutrient distributions, except resulting in greater extractable K at 0–0.05 m and greater nitrate at 0–0.15 m. Few changes in soil-profile distributions were observed for extractable S, Ca, Mg, and Na. Long-term continuous use of NT on this fine-textured, high-fertility (except for N) soil had no apparent adverse effects on nutrient distributions relative to CT, but enhanced conservation and availability of P, K, Zn, Fe, Mn, and Cu near the soil surface where crop roots proliferate.  相似文献   

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