共查询到20条相似文献，搜索用时 78 毫秒
1.
为探讨茯砖茶对高脂诱导的非酒精性脂肪肝病（Non-alcoholic fatty liver disease,NAFLD）大鼠肠黏膜屏障及肝损伤的保护作用,将SD雄性大鼠随机分为正常组、NAFLD组、茯砖茶低、高剂量组,每组8只。通过分析大鼠的采食量、体重变化、血脂指标、肝脏指标及病理切片来评估NAFLD大鼠模型的构建情况及茯砖茶的干预作用。通过分析空肠与结肠病理及PAS染色切片、绒毛高度/隐窝深度值（V/C值）、血清二胺氧化酶（DAO）和脂多糖（LPS）活力评估肠道完整性和通透性;通过分析空肠上皮间淋巴细胞（JIL）数量和血清肿瘤坏死因子（TNF-α）活力评估肠道相关炎症。结果表明,茯砖茶能减少NAFLD大鼠的采食量和体重,降低血脂水平,减少血清中谷草转氨酶（AST）和谷丙转氨酶（ALT）活力,并抑制肝脏的脂质聚集和炎细胞浸润。茯砖茶还降低了血清炎症因子TNF-α和血清LPS的水平,并使血清DAO活力上升。同时,茯砖茶抑制了NAFLD大鼠空肠和结肠绒毛中发生的炎细胞浸润现象,减少了空肠绒毛脱落、断裂、稀疏和紊乱现象,增加了空肠绒毛V/C值,降低了空肠上皮间淋巴细胞数量,增加了空肠和结肠的杯状细胞数量。这些结果说明,茯砖茶能够有效的改善高脂饮食诱导的肠道黏膜屏障及肝损伤,起到防治NAFLD的作用。 相似文献
2.
非酒精性脂肪肝是以肝实质细胞发生脂肪堆积和脂肪变性为主要特征,并伴随机体脂质代谢紊乱的一种获得性代谢疾病。以高脂饲料喂养大鼠,建立非酒精性脂肪肝大鼠模型;以低、中、高剂量（10、50、100 mg·kg-1）表没食子儿茶素没食子酸酯（EGCG）分别灌胃模型大鼠,探讨茶叶中EGCG对非酒精性脂肪肝大鼠的调脂保肝作用。结果表明,与模型组相比,EGCG能显著降低血清中甘油三酯（TG）、总胆固醇（TC）和低密度脂蛋白-胆固醇（LDL-C）含量,降低丙氨酸氨基转移酶（ALT）和天冬氨酸转氨酶（AST）活性水平,提高高密度脂蛋白-胆固醇（HDL-C）的含量,改善大鼠肝脏氧化应激状态。此外,肝脏病理切片显示,EGCG能减少肝细胞中脂滴的形成,同时脂质代谢相关基因表达量分析显示,EGCG能调理肝脏的脂质代谢。综合以上实验结果表明,EGCG能显著改善非酒精性脂肪肝大鼠的脂质代谢紊乱和脂肪性肝损伤。 相似文献
3.
脂肪组织类型与人体代谢密切相关,通过饮食或营养干预将白色脂肪细胞转变为产热的米色脂肪细胞是一种减少脂肪积蓄、调节代谢的安全策略。目前关于白色脂肪组织米色化作用的研究多聚焦于肥胖群体,为探究EGCG对非肥胖代谢紊乱群体的内脏白色脂肪组织米色化的诱导作用及相关机制,采用非肥胖型自发性2型糖尿病模型Goto-Kakizaki(GK)大鼠,给予每日高脂饮食,并进行40 mg·kg-1和80 mg·kg-1 EGCG灌胃干预,检测GK大鼠的体质量、摄食量、脂肪组织细胞形态及米色化相关基因表达水平、UCP1蛋白表达水平,并进行转录组测序。结果表明,80 mg·kg-1 EGCG灌胃干预对GK大鼠摄食量和体质量无明显影响,但能够促使脂肪细胞呈现向多房型脂肪细胞转变趋势,并显著上调米色化相关的Pparg、Ppargc1a、Ucp1基因表达水平和UCP1蛋白表达水平,具有诱导高脂饮食GK大鼠内脏附睾白色脂肪组织米色化的作用,且表现出调节脂质代谢的潜力。结合转录组分析结果表明,EGCG对高脂饮食GK大鼠白色脂肪组织米色化的诱导作用机制可能与... 相似文献
4.
5.
EGCG是一种抗氧化、抗炎症的天然活性成分，目前关于EGCG的抗氧化和抗炎症作用在糖尿病肾损伤中的作用及调节机制研究较少。采用不同剂量的EGCG干预非肥胖型糖尿病Goto-Kakizaki(GK)大鼠以探究EGCG对糖尿病大鼠肾损伤的作用和机制。试验周期为4周，监测试验期内大鼠的平均体质量和日平均摄食量，并在试验结束后取血清和肾脏组织，检测大鼠肾功能、肾脏病理指标、氧化应激和炎症指标以及Nrf2-Keap1/MAPK信号通路相关基因表达水平。试验表明，EGCG能够有效改善GK大鼠肾脏形态损伤，显著降低血肌酐、尿素氮和肾脏丙二醛含量，提高肾脏超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶等抗氧化酶活性，抑制促炎因子单核细胞趋化蛋白-1和白介素-1β的释放水平，调节与抗氧化应激相关的Nrf2-Keap1/MAPK信号通路的Nrf2、Keap1、JNK、NF-κB、P38基因表达水平，且在试验范围内，高剂量的改善效果优于低剂量。以上结果表明，EGCG对大鼠糖尿病导致的肾损伤有一定的改善作用，其作用机理可能与Nrf2-Keap1/MAPK信号通路介导的抗氧化应激有关。 相似文献
6.
为探究EGCG对3T3-L1前脂肪细胞分化的影响及其分子机制,以3T3-L1前脂肪细胞为材料,分别添加不同浓度EGCG(0μM、5μM、10μM、50μM、100μM),通过油红O染色检测EGCG对3T3-L1脂肪细胞分化过程中脂质积累的影响,同时采用RT-PCR和免疫印迹检测不同浓度EGCG对PPARγ、FAS、PI3K、FOXO1的mRNA及蛋白表达的影响.油红O染色结果表明,EGCG可抑制3T3-L1前脂肪细胞分化,阻碍脂质积累,并具有浓度效应,EGCG浓度越高抑制作用越明显;EGCG能够直接抑制PPARγ和FAS的表达,同时抑制P-AKT、P-PI3K以及P-FOXO1的表达.表明EGCG通过介导PI3K/AKT-FOXO1级联信号通路,下调具有促进前脂肪细胞分化功能的PPARγ和FAS的表达,从而抑制前脂肪细胞分化,阻碍脂肪生成. 相似文献
7.
8.
湖北青砖茶对IBS-D模型大鼠肠道敏感性的影响 总被引：1，自引：0，他引：1
研究了湖北青砖茶对腹泻型肠易激综合征(IBS-D)模型大鼠肠道的影响。结果表明,与正常组相比,模型组的痛阈值明显降低,血清中炎症因子前列腺素2(PGE2)、肿瘤坏死因子(TNF-α)、类胰蛋白酶的含量明显增加;与模型组比较,湖北青砖茶低剂量组、高剂量组能够显著升高痛阈值,而血清中炎症因子PGE2、TNF-α、类胰蛋白酶的含量显著降低,且具有剂量依赖性。由此表明湖北青砖茶可降低IBS-D模型大鼠的肠道敏感性,其作用机制可能与血清中PGE2、TNF-α和类胰蛋白酶含量的降低有关。 相似文献
9.
湖北青砖茶减肥作用研究 总被引：4，自引：1，他引：3
本文探讨了当年湖北青砖和10年青砖茶对肥胖大鼠的减肥作用。结果表明:与肥胖模型组比较,当年青砖茶和10年青砖茶都能极显著降低肥胖大鼠体重、Lee’s指数和脂肪系数,能极显著降低大鼠血清和肝脏中TC、TG、MDA的含量,提高HDL-C、SOD和GSH-PX的含量。脂肪细胞观察表明,喂饲当年青砖茶和10年青砖茶大鼠的脂肪细胞中脂滴含量明显减少,脂肪细胞直径明显变小。说明湖北青砖茶具有明显的减肥抗氧化作用,当年青砖茶的减肥效果具有剂量依赖关系,10年青砖茶减肥效果优于当年青砖茶。 相似文献
10.
将50只KM雄性小鼠按每组10只随机分为正常组、谷氨酰胺组及L-茶氨酸低、中、高剂量组,连续灌喂28 d,探究L-茶氨酸对小鼠肠道形态结构及游离氨基酸的影响。结果表明,与正常组相比,小鼠肠道绒毛高度随L-茶氨酸剂量的增大而增加,各剂量组的十二指肠绒毛高度和中、高剂量组的空肠绒毛高度及其绒毛高度与隐窝深度的比值均达显著水平(P<0.05),血清游离氨基酸含量及中、高剂量组的尿素氮含量显著降低(P<0.05),高剂量组的碱性磷酸酶、总蛋白含量显著升高(P<0.05);L-茶氨酸对不同肠道游离氨基酸含量的影响随剂量的增大而升高,其中,十二指肠中的各茶氨酸剂量组的全部必需氨基酸和大部分非必需氨基酸显著升高(P<0.05),且高剂量组的必需氨基酸含量显著高于低剂量组(P<0.05);空肠中的低剂量组的必需氨基酸Thr、Lys、Ile及中、高剂量组的全部必需氨基酸含量显著升高,且中、高剂量组中除Cit外的其他氨基酸含量均显著高于低剂量组(P<0.05);回肠中的低、中、高剂量组的全部必需氨基酸及大部分非必需氨基酸含量显著降低,且中、高剂量组中除GABA外的其他氨基酸含量显著高于低剂量组(P<0.05)。结果表明,L-茶氨酸可通过增加肠道绒毛高度、提高绒毛高度与隐窝深度比值来改善小鼠肠道形态结构,同时可促进十二指肠、空肠对氨基酸的吸收利用。 相似文献
11.
《Plant Production Science》2013,16(2):189-198
AbstractGrain number per spike of wheat is lower in early sowing than in the conventional standard cultivation in Yamaguchi, Prefecture, Japan. Components of the grain number per spike in five cultivars were analyzed with respect to temperature during the spike development period throughout three growing seasons 2001/2002, 2002/2003 and 2003/2004 to find the cause of the problem of early sowing cultivation. The plants sown in early-October and late-November were called the early sown group and the standard group, respectively, in the following. Three of the five cultivars, Hokushin, Akitakko and Nanbukomugi, showed a strong winter habit, which requires very cold temperatures for spike differentiation. The other two cultivars, Iwainodaichi and Airakomugi, had a moderate winter habit. Grain number per spike and grain yield were decreased by early-sowing (compare with the standard group) in almost all cultivars throughout the three growing seasons. The three cultivars which had a strong winter habit had fewer spikelets per spike in the early-sown group than in the standard group. The other two cultivars which had a moderate winter habit had fewer grains per spikelet in the early-sown group. The higher the temperature during the spikelet formation phase, which is from flag leaf initiation to terminal spikelet initiation, the higher the number of spikelets per spike in the standard group. The spikelet number per spike in the early-sown group increased with the increase in productive tillers under fertile conditions. Such conditions also increased the grain number per spike. 相似文献
12.
《Journal of Crop Improvement》2013,27(2):415-432
Summary This paper reviews the effects of allelopathic interactions in agroecosystems in Spain on plant physiological activity and their ecological advantages. The phenological stage of growth of donor plants and the effective allelochemicals in the soil solution while studying the role of phenolic compounds were highlighting. Finally possible future prospects and conclusions regarding weed control by allelochemi-cals under integrated crop management strategies are discussed. 相似文献
13.
14.
以翔实的资料对2009年中国稻米生产、消费、进出口、供求平衡、库存、价格走势、购销政策和国际稻米供求等情况进行了全面地分析,对影响2010年稻米市场价格走势的各种因素进行了深入研究,并在此基础上对2010年我国稻米市场价格走势进行了预测,结论是我国稻米价格仍呈上行趋势。 相似文献
15.
Summary Diurnal changes in net photosynthetic rate were measured in a furrow-irrigated potato crop and in a riverbed crop where the
water table was always maintained at 20–28 cm from the soil surface. In the irrigated crop, the photosynthetic rate during
mid-afternoon was about half the peak rate observed at noon. This reduction was accompanied by a near tripling of stomatal
resistance, a 45% reduction in transpiration, and a 5-fold increase in the difference between leaf and air temperatures. No
such changes were observed in the riverbed crop where the photosynthetic rate remained nearly constant at about 0.9 mg m−2 s−1 between 9 a.m. and 4 p.m. Tuber yield in the riverbed crop was about 30% higher than in the irrigated crop. 相似文献
16.
17.
体细胞无性系变异是一种重要的细胞工程育种技术,其应用于棉花育种的前提是建立高效的组织培养诱导再生植株体系.本文简要综述了棉花离体诱导植株再生研究的现状,重点讨论了棉花体细胞无性系变异的表现、利用方法及机理,提出了当前研究存在的问题并对今后的研究进行了展望. 相似文献
18.
19.
20.
Corné Kempenaar Thomas Been Johan Booij Frits van Evert Jean-Marie Michielsen Corné Kocks 《Potato Research》2017,60(3-4):295-305
Precision agriculture is a farming management concept based on observing, measuring and responding to inter- and intra-field variability in crops. In this paper, we focus on responding to intra-field variability in potato crops and analyse variable rate applications (VRAs). We made an overview of potential VRAs in potato crop management in The Netherlands. We identified 13 potential VRAs in potato, ranging from soil tillage to planting to crop care to selective harvest. We ranked them on availability of ‘proof of concept’ and on-farm test results. For five VRAs, we found test results allowing to make a cost-benefit assessment. These five VRAs were as follows: planting, soil herbicide weed control, N side dress, late blight control and haulm killing. They use one of two types of spatial data: soil maps or biomass index maps. Data on costs and savings of the VRAs showed that the investments in VRAs will pay off under practical conditions in The Netherlands. Savings on pesticide use and N-fertilizer use with the VRAs were on average about 25%, which benefits the environment too. We foresee a slow but gradual adoption of VRAs in potato production. More VRAs will become available given ongoing R&D. The perspectives of VRAs in potatoes are discussed. 相似文献