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1.
以丛生芽率为指标,将农杆菌介导的大豆未成熟种子子叶节转化与成熟种子转化体系进行比较,明确了诱导过程中草甘膦筛选浓度、未成熟种子生理状态,改进大豆外植体的获得方式并减少了组培环节,建立了未成熟种子子叶节转化体系。利用Jack黄熟后期的种子作为受体材料,在15 mg·L-1的草甘膦筛选浓度下获得了9株PCR检测阳性植株并且生长正常可育,T0代经测序及Southern Blot分析,进一步证明外源基因已整合进大豆基因组中,其中2个株系的T1代植株经PCR检测符合3∶1的分离比,且表型鉴定筛选出抗性转基因植株,为抗草甘膦转基因大豆育种提供了新材料。  相似文献   

2.
麦田恶性杂草节节麦发芽特性研究   总被引:2,自引:0,他引:2  
为深入了解节节麦的发芽特性,从种子吸水、萌发温度以及播种深度等方面对节节麦和小麦进行了对比研究。结果表明,节节麦与小麦种子吸水进程均可分为3个时期,即快速吸水期、缓慢吸水期和吸水停滞期。小麦吸水主要集中在前期,而节节麦吸水分散于前、中期;在整个吸水过程中,无论是吸水量还是吸水率,小麦普遍高于节节麦。温度对节节麦和小麦发芽率的影响也具有较大差异,在5~25 ℃范围内,节节麦发芽率随着温度的升高而提高,不同温度间具有显著差异;而小麦在不同温度条件下发芽率保持一致,不受影响。在大田条件下,节节麦的出苗率受播种深度影响较大,随播种深度的增加出苗率显著降低,二者呈极显著负相关。  相似文献   

3.
几种除草剂在冬季亚麻中的应用   总被引:2,自引:1,他引:1  
利用二甲四氯、敌草隆、精喹禾灵及宝成干除草剂防治冬季亚麻田的阔叶杂草和禾本科杂草的药效试验表明:56%二甲四氯钠盐50g+50%敌草隆50g/667m2及56%二甲四氯钠盐50g+5%精喹禾灵乳油50ml/667m2两种处理,对麻田杂草防效较好.  相似文献   

4.
为研究开发新型、高效、安全的棉花化学打顶剂,筛选适于棉花的化学打顶剂配方,选择杀菌剂戊唑醇、除草剂二甲戊灵和抑芽剂氟节胺3种药剂,于2020年在河南安阳进行棉花化学打顶试验,研究其化学打顶效果。结果表明:430 g·L-1戊唑醇悬浮剂(SC)258 g·hm-2(有效成分用量,下同)+330 g·L-1二甲戊灵乳油(EC)990 g·hm-2+25%(质量分数,下同)氟节胺SC 300 g·hm-2混配处理抑制棉花株高和增产效果最佳,可以达到人工打顶的效果;其次为430 g·L-1戊唑醇SC 516 g·hm-2+25%氟节胺SC 300 g·hm-2处理;而330 g·L-1二甲戊灵EC 1 485 g·hm-2+25%氟节胺SC 300 g·hm-2处理和25%氟节胺SC 500 g·hm-2单剂处理可在一定程度上提高棉花产量,但对棉花的打顶效果远不及人工打顶,仍须优化配方和确定合适剂量。因此,在本研究区宜用430 g·L-1戊唑醇SC 258 g·hm-2+330 g·L-1二甲戊灵EC 990 g·hm-2+25%氟节胺SC 300 g·hm-2或430 g·L-1戊唑醇SC 516 g·hm-2+25%氟节胺SC  300 g·hm-2进行棉花化学打顶来代替传统人工打顶。  相似文献   

5.
为了给节节麦的遗传研究提供信息,对来自中东地区及中国陕西、河南及新疆的214份节节麦材料的籽粒性状的变异及相关性进行了分析。结果表明,中东地区和中国新疆的节节麦籽粒性状的变异系数均高于河南和陕西的节节麦;通过对四个地区节节麦籽粒的粒长、粒宽、粒厚和粒重的平均值做单因素方差分析及S-N-K多重比较,并对上述四个形态指标的平均值做聚类分析,结果显示,新疆节节麦单独聚为一类,中东地区、陕西和河南的节节麦聚为一类,其中河南和陕西的相似性更为一致。同时对四个地区节节麦籽粒的粒形(长、宽、厚)与其粒重进行了相关性分析,结果显示,中东地区和新疆节节麦粒重主要取决于其粒长,河南节节麦粒重则主要取决于其粒宽,而陕西节节麦粒重与其粒长呈负相关,主要取决于其粒宽和粒厚。  相似文献   

6.
在新疆伊犁进行了二甲四氯钠与烯草酮混用防除亚麻田一年生阔叶杂草和禾本科杂草的田间试验,结果表明:药后30 d,二甲四氯钠与烯草酮混用对亚麻田杂草的株防效和鲜重防效均在88%以上,明显高于二者单用时的防效,且持效期长,一次施药能控制亚麻田生育期大多数杂草,对亚麻安全,两者混用是有效控制亚麻田杂草的理想措施。  相似文献   

7.
为揭示中国节节麦的遗传多样性并发掘可能具有独特变异的节节麦种质资源,采用ISSR标记对75份中国节节麦的遗传多样性进行分析。结果表明,筛选出的9条ISSR引物共检测得到155个位点,多态性位点(138个)百分率为89.31%。Nei’s多样性指数(He)、Shannon’s信息指数(I)、基因分化系数(Gst)和基因流(Nm)分别为0.273 4、0.415 2、0.138 5和3.11,表明中国节节麦有较高的遗传多样性,群体间有中等程度的遗传分化。基于简单相似系数的UPGMA聚类分析表明,除5份河南和陕西节节麦聚类形成独立的分支Group 2(T078、T102和SC1)和Group 3(SX38和T006)外,绝大多数节节麦均聚类形成较大的分支Group 1,且在Group 1中,除4份节节麦(T002、T023、XJ6和XJ49)外,绝大多数节节麦均依据地理分布分别形成了黄河流域节节麦亚组和新疆节节麦亚组,在遗传距离约0.77处,又可依次分为河南、陕西和新疆节节麦小分支。二维主成分分析、群体的遗传一致度和分子变异分析也表明了类似的结果,同属于黄河流域节节麦亚组的河南、陕西节节麦遗传一致度(S=0.971 8)较高,群体内个体差异是中国节节麦变异的主要原因,但两大亚组和三居群的遗传差异分别占总变异的18.57%和10.38%。可见,不同节节麦的地理分布和生境差异,是导致中国节节麦居群遗传分化的主要原因,而聚类分析中单独形成独立分支Group 2和Group 3的5份黄河流域节节麦,可能是具有独特遗传变异的种质资源。  相似文献   

8.
王子光 《中国棉花》2012,39(3):32-33
安徽沿江棉区以种植棉花、油菜、小麦为主,一般为两熟制。在当地广泛使用的除草剂类型主要有:(1)灭生性除草剂有10%草甘膦、62%草甘膦、40%草甘膦、百草枯等;(2)阔叶除草剂有苯磺隆、乙羧氟草醚、二甲四氯等;(3)禾本科除草剂有高效盖草能、精奎禾灵;(4)芽前除草剂有乙草胺、金都尔、床草净、野老等。灭生性除草因省工省时、除草效果好,备受农户青睐,在棉花生产中至少使用2次,即移栽棉花前使用一次,封行前再使用一次。  相似文献   

9.
节节麦含有丰富的优良基因,可用于小麦的品质改良和产量提高。为了揭示L1和L2谱系节节麦的遗传差异和杂种优势,对L1、L2谱系节节麦开展人工杂交,采用SSR分子标记鉴定杂种F1的真实性,对其花粉活力和F1减数分裂时染色体配对情况进行观察,并对其主要农艺性状进行了调查和统计分析。结果表明,长期的地理隔离使L1和L2谱系节节麦产生一定的遗传分化,但尚未形成生殖隔离。F1的花粉活力和同源染色体配对正常,绝大部分F1的中期同源染色体联会形成环状染色体,少数出现棒状染色体。对8个杂交组合的16个主要农艺性状的统计分析表明,L1、L2谱系节节麦的百粒重和分蘖数存在杂种优势,尤其是杂交组合AY26×AY46和XJ47×AY46,其百粒重(IH>143%,OPH>21%)和分蘖数(IH>187%,OPH>57%)存在显著的杂种优势。有望通过对这2个杂交组合的进一步深入研究,揭示节节麦杂种优势的分子机理,为其在小麦产量育种中的有效利用奠定基础。  相似文献   

10.
节节麦(Aegilops tauschii,DD)是六倍体普通小麦D基因组的祖先,其自然类群中含有丰富的抗逆、高产基因,利用其与四倍体硬粒小麦合成的六倍体小麦在现代小麦育种中得到了愈来愈多的应用。本课题在野生节节麦类群中发现了大穗、大粒材料AT462,利用其作母本与节节麦材料AT18(强分蘖)杂交;构建了F2、F3群体,通过调查亲本和群体单株的穗长、小穗数、粒长、粒宽和粒重等表型,对这些穗部性状进行了相关性分析和遗传分析。结果表明:(1)在F2和F3群体中,粒重、粒长与穗长之间不存在显著相关性,而且穗长与粒宽之间在两个群体中的平均相关系数绝对值小于0.1,粒重与小穗数之间的相关系数绝对值小于0.2,表明节节麦大粒相关性状不受穗长的影响,受小穗数影响也较小;(2)采用F2单世代分离分析的方法对节节麦AT462×AT18的F2群体大穗、大粒相关性状进行遗传分析,其中穗长受2对具有加性效应的主效基因控制;粒重和小穗数均同时受2对基因的加性效应、显性效应以及互作效应控制,其中加性效应占主导地位;粒长、粒宽均受2对基因的加性效应、显性效应以及互作效应控制,且三种效应较为均衡。这说明控制节节麦粒重、穗长、小穗数等产量性状相关基因的加性效应在遗传中占主导地位,在育种中较易利用,且其主效基因的遗传力达0.9。  相似文献   

11.
Sorghum and millet phenols and antioxidants   总被引:5,自引:2,他引:5  
Sorghum is a good source of phenolic compounds with a variety of genetically dependent types and levels including phenolic acids, flavonoids, and condensed tannins. Most sorghums do not contain condensed tannins, but all contain phenolic acids. Pigmented sorghums contain unique anthocyanins that could be potential food colorants. Some sorghums have a prominent pigmented testa that contains condensed tannins composed of flavan-3-ols with variable length. Flavan-3-ols of up to 8–10 units have been separated and quantitatively analyzed. These tannin sorghums are excellent antioxidants, which slow hydrolysis in foods, produce naturally dark-colored products and increase the dietary fiber levels of food products. Sorghums have high concentration of 3-deoxyanthocyanins (i.e. luteolinidin and apigenidin) that give stable pigments at high pH. Pigmented and tannin sorghum varieties have high antioxidant levels that are comparable to fruits and vegetables. Finger millet has tannins in some varieties that contain a red testa. There are limited data on the phenolic compounds in millets; only phenolic acids and flavones have been identified.  相似文献   

12.
种子加工、检验理论与技术现状及思考   总被引:3,自引:0,他引:3  
种子加工及检验是种子向市场流通的关键,是商业化育种中赋予种子商品属性不可或缺的环节。种子加工、检验理论与技术的研究是完善种业产业链、实现规模化商业育种、夯实“育繁推一体化”种业科学的重要一环。中国的种业科学技术体系正在形成与完善之中,受制于行业发展水平,种子加工及检验理论与技术相对薄弱。本文回顾了种子加工及检验理论与技术研究发展历程,提出了完善中国种业科学发展的策略与建议。围绕种业发展的需要,在做好品种优质化繁育的基础上,分析种子加工及检验理论与技术的新方向和新需求,加强种业应用性研究,建立先进的种子质量检验体系,研制适合现代种业发展需求的种子加工设备,打造种子加工产业标准化生产体系,完善种业科学技术学科建设,促进种业产业持续健康发展。  相似文献   

13.
Summary Between 1993 and 1998 205 different potato cultivars and 1220 accessions/genotypes of wild and cultivated potato species from the IPK Genebank Gatersleben were evaluated. Parameters interesting for starch isolation and especially for the use of starch were determined. Altogether, there was a higher variability in wild potato species than in cultivated potatoes for all characteristics investigated: dry matter content, starch content, protein content, amylose content and mean particle diameter of starch granules.  相似文献   

14.
Seed processing and testing hold the key to the market circulation of seeds, and are an indispensable link in commercial breeding to endow seeds with commodity attributes. The research of seed processing and testing theory and technology is an important link in improving the seed industry chain, realizing large-scale commercial breeding and consolidating the seed industry science of ‘integration of breeding, propagation and promotion’. The scientific and technological system of seed industry in China is being formed and perfected, which is subject to the development level of the industry. The theory and technology of seed processing and testing are relatively weak. We reviewed the development of seed processing and testing theory and technology, and put forward strategies and suggestions to improve the sound development of China’s seed industry. In order to meet the needs of seed industry development and on the basis of high quality breeding of varieties, the new directions and demands of seed processing and testing theory and technology were analyzed. We will work to strengthen the applied research of seed industry, establish advanced seed quality inspection system, improve seed processing equipment suitable for the development needs of modern seed industry, establish standardized production system of seed processing industry, develop the scientific and technological disciplines of seed industry, and promote the sustainable and healthy development of seed industry.  相似文献   

15.
Summary

Arbuscular mycorrhizae were inoculated into phosphorus-deficient soil fertilized with either organic or chemical fertilizer with cucumber (Cucumis sativus L.) as the first crop and lettuce (Lactu-ca sativa L.) as the second crop but without additional fertilization and AM inoculation. AM increased dry matter and fruit yield of cucumber significantly in the unfertilized, organic-fertilized and P-deficient plants compared with the fully chemical-fertilized plants. AM inoculation increased the available phosphorus in plant and soil by around 30% for all treatments except for those chemically-fertilized. The rate of AM infection did not differ significantly among the fertilization treatments, but the infection intensity was higher in unfertilized, organic-fertilized and phosphorus-deficient treatments than chemical-fertilized treatment. The residual effects of AM-inoculated to cucumber were evident for lettuce in all pre-treatments that were unfertilized and un-inoculated for the second cropping. Without P-fertilization, neither crop could grow optimally even when the soil was inoculated with AM, suggesting that AM could not serve as a substitute for phosphorus fertilizer. However, the other beneficial effects of AM on crop growth and yield could not be fulfilled with phosphorus fertilizer.  相似文献   

16.
种子加工及检验是种子向市场流通的关键,是商业化育种中赋予种子商品属性不可或缺的环节。种子加工、检验理论与技术的研究是完善种业产业链、实现规模化商业育种、夯实"育繁推一体化"种业科学的重要一环。中国的种业科学技术体系正在形成与完善之中,受制于行业发展水平,种子加工及检验理论与技术相对薄弱。本文回顾了种子加工及检验理论与技术研究发展历程,提出了完善中国种业科学发展的策略与建议。围绕种业发展的需要,在做好品种优质化繁育的基础上,分析种子加工及检验理论与技术的新方向和新需求,加强种业应用性研究,建立先进的种子质量检验体系,研制适合现代种业发展需求的种子加工设备,打造种子加工产业标准化生产体系,完善种业科学技术学科建设,促进种业产业持续健康发展。  相似文献   

17.
通过比较分析芸薹属多倍体复合种与其祖先二倍体基本种在花柱、柱头大小、角果长度、宽度、种子直径 及种子干重变化上的异同,发现多倍体复合种在这些发育性状上表现出处于两个祖先二倍体亲本之间,或只偏向 于其中一个祖先亲本。同一个多倍体品种在不同发育性状上具有不同的表现,即在某一发育性状上偏向于其祖先 亲本之一,而在另一发育性状上偏向其另一祖先亲本。  相似文献   

18.
对云南保山、德宏、普洱等地咖啡病虫害开展调查研究,结果表明:咖啡锈病(Hemileia vastatrix Berk et Br.)、炭疽病(Colletotrichum coffeanum Noack)、褐斑病(Cercospora coffeicola Berk. et Cooke)、幼苗立枯病(Rhizoctonia solani Kuhn.)、旋皮天牛(Acalolepta cervina Hope)、灭字虎天牛(Xylo  相似文献   

19.
Pea (Pisum sativum L.) and oat (Avena sativa L.) were grown as sole and mixed crops in various densities under two different tillage systems on a loess soil near Göttingen/Germany in a 2-year field experiment (2002/2003). In the conventional tillage system a mouldboard plough (CT) was used and in the minimum tillage system a rotary harrow (MT) was employed. The effect of crop density and tillage system on the grain dry matter and grain N yields, N2 fixation and soil N uptake were determined to address the following questions: (i) which mixture compositions exhibit the highest grain yields compared to the sole crops, (ii) which mixture compositions also fix a high level of N2 and leave low levels of residual inorganic soil N after harvest, and (iii) whether the intercrop advantage is influenced by the tillage system. For (i) the result in 2002 showed that the highest grain yields of both sole cropped pea and oat and intercropped pea and oat were achieved at the highest densities. In 2003, when the inorganic soil N content was higher and weather conditions were warmer and drier, grain yields were significantly higher than in 2002, but sole as well as intercropped pea and oat gave their highest grain yields at lower densities. For both years and tillage systems, the highest intercrop advantages were achieved in mixtures with densities above the optimal sole crop densities. The result for (ii) was that a distinctly higher proportion of nitrogen was derived from the atmosphere (Ndfa) by intercropped pea than by sole cropped pea. However, the uptake of soil N by intercropped pea and oat was not reduced in comparison with that of sole cropped oat as the decrease in the uptake of N from the soil by oat at lower oat densities in the mixture was compensated for by the soil N uptake of pea. Additionally, the Nmin-N content of the soil following the mixtures and sole cropped oat did not differ, especially in the deeper soil layers because oat in mixture was forced to take up more soil N from deeper layers. Therefore, the risk of soil N losses through leaching after mixtures was lower compared to sole cropped pea. The tillage system (iii) had no significant influence on grain yield and soil N uptake, but N2 fixation and the competitive ability of intercropped pea were higher under CT than with MT. An additional result was that intercropping led to a significantly increased grain N content of both pea and oat compared to the sole crops. The increase in grain N content from sole to intercrop was from 3.30 to 3.42% for pea and from 1.73 to 1.96% for oat as a mean for both years and tillage systems. The present study confirms that growing pea and oat as intercrops highlights potential economic and environmental benefits which still need to be understood in more detail in order to exploit intercropping to a greater extent.  相似文献   

20.
Soil sulphur deficiency, which is increasingly prevalent in Western Europe, lowers wheat yields, and also affects the gluten quality of the flour. Differences in S availability may change the proportion of S-poor to S-rich gliadins and glutenin subunits. This may cause unpredictable and unwanted variations in wheat quality. The combined effects of nitrogen (N) and sulphur (S) fertilisers and split application of S and N on wheat gluten quality and composition were investigated. The results revealed effects of S fertilisation on dough quality. At high N fertilisation levels significant responses to S fertilisation were found which emphasised the need for precision application of S in intensive wheat production systems. Protein fractionation by SE-FPLC showed that quality differences were associated with changing proportions of high Mr polymeric proteins. Changes in protein composition of salt soluble proteins were also confirmed by proteomics. Glyceraldehyde-3-phosphate dehydrogenase and one of the serpin protein spots increased at high N, combined with the lower S level. The enzymes also increased in samples with increased S fertilisation combined with low N, but was not changed at higher N levels. Furthermore, at high N the serpin protein spot, and also a 27 K protein and one unidentified protein spot decreased with increasing S.  相似文献   

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