共查询到18条相似文献，搜索用时 265 毫秒
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利用2b-RAD技术对119尾黄条鰤(Seriola lalandi)个体进行测序,共获得黄条鰤SNP分子标记26665个,对黄条鰤个体的体质量和全长这2个重要生长性状进行全基因组关联分析,筛选与体质量和全长性状相关的SNP位点和候选基因。结果显示,黄条鰤体质量性状中共筛选到17个体质量显著关联的SNP位点,找到17个可能的候选基因,全长性状共筛选到12个潜在显著关联位点,找到12个可能的候选基因。利用KEGG数据库对可能的候选基因进行Pathway分析,得知候选基因主要参与了细胞或组织生长发育相关的代谢通路调控过程,可能是影响黄条鰤生长性状密切相关的重要候选SNP位点和功能基因,结果可为今后黄条鰤种质资源可持续利用和育种提供遗传信息资料积累。 相似文献
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《渔业科学进展》2021,42(2)
利用2b-RAD技术对119尾黄条(Seriola lalandi)个体进行测序,共获得黄条SNP分子标记26665个,对黄条个体的体质量和全长这2个重要生长性状进行全基因组关联分析,筛选与体质量和全长性状相关的SNP位点和候选基因。结果显示,黄条体质量性状中共筛选到17个体质量显著关联的SNP位点,找到17个可能的候选基因,全长性状共筛选到12个潜在显著关联位点,找到12个可能的候选基因。利用KEGG数据库对可能的候选基因进行Pathway分析,得知候选基因主要参与了细胞或组织生长发育相关的代谢通路调控过程,可能是影响黄条生长性状密切相关的重要候选SNP位点和功能基因,结果可为今后黄条种质资源可持续利用和育种提供遗传信息资料积累。 相似文献
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《南方水产科学》2018,(6)
为了探讨HSC70-1基因对大口黑鲈(Micropterus salmoides)生长性状的影响作用,开发与生长性状相关的分子标记,该研究采用PCR技术扩增得到HSC70-1基因的cDNA和DNA全序列,编码区cDNA序列长1 953 bp,编码650个氨基酸,其DNA全序列长3 390 bp,包含8个外显子和7个内含子。采用直接测序法在该基因上筛选到4个单核苷酸多态性(SNP)位点,分别位于核苷酸序列中的第821、第1 105、第1 200和第2 804碱基处,且处于内含子上。SNP标记与性状的关联分析结果表明,C-821G位点上CC基因型个体的体质量和全长均显著高于CG和GG基因型(P0.05)。A-2804T位点上TT基因型个体的体质量和全长均显著高于AT和AA基因型(P0.05)。其他位点不同基因型个体间的生长性状均不存在显著差异(P0.05)。通过合并基因型发现,双倍型D1 (CC--CCTT)的各个生长性状表现最优,在体高和全长上显著高于D5。C-821G、A-2804T和D1与生长性状显著相关,可作为大口黑鲈分子标记辅助育种的候选标记。 相似文献
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为筛选刀鲚(Coilia ectenes)生长性状遗传改良的分子遗传标记,基于现有刀鲚转录组数据,利用基因克隆技术获得养殖刀鲚肌肉生长抑制素(myostatin,MSTN)基因的全长序列,采用PCR产物直接测序和SNaPshot技术分别进行基因多态性的筛选和分型,并将不同基因型与刀鲚的生长性状(体长、体高和体质量)进行关联分析。结果显示,养殖刀鲚MSTN基因的DNA序列主要由2个内含子和3个外显子组成,经SNP位点筛选,仅在第1内含子上筛选到2个颠换突变位点(g.564G>T和g.755G>T)和1个转换突变位点(g.786C>T);将3个SNP位点与生长性状进行关联分析发现,SNP位点g.786C>T与刀鲚的体长、体高和体质量呈显著性负相关,具有TT基因型个体的体长、体高和体质量显著低于CC型和CT型(P<0.05),而SNP位点g.564G>T和g.755G>T的不同基因型仅与刀鲚的体高具有一定程度的相关性;3个SNP位点对养殖刀鲚的群体遗传分析显示,刀鲚养殖群体的观测杂合度(H O)、期望杂合度(H E)和多态信息含量(PIC)分别为0.280~0.480、0.332~0.455和0.277~0.351,3个SNP位点均属于中度多态(0.25T有望作为剔除劣质刀鲚繁育亲本的候选分子标记。 相似文献
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缢蛏EGFR基因内含子1内SNP位点多态性与生长性状相关性 总被引：1，自引：1，他引：0
为研究缢蛏表皮生长因子受体基因(epidermal growth factor receptor,EGFR)单核苷酸多态性(single nucleotide polymorphism, SNP)与生长性状(壳长、壳宽、壳高和体质量)的相关性。本实验利用直接测序法从缢蛏EGFR基因的第一个内含子序列中共筛选到17个SNP位点。卡方检验结果显示,在17个位点中,有13个位点符合Hardy-Weinberg平衡,位点多态性检测显示17个位点中有10个位点表现为中等多态性(0.25PIC0.5)。利用一般线性模型(general linear model,GLM)及多重比较对缢蛏EGFR基因中17个SNPs的多态性与生长性状(壳长、壳宽、壳高和体质量)进行相关性分析,结果显示,16个SNP位点均与缢蛏的壳长、壳宽、壳高及体质量呈显著性相关。由此可见,EGFR基因可作为缢蛏生长性状改良的候选辅助分子标记,并且为进一步研究其生长相关功能奠定基础。 相似文献
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为了揭示大菱鲆(Scophthalmus maximus)体重和体尺性状的分子遗传机制,探寻用于改良目标性状的分子标记及候选基因,本研究以大菱鲆育种群体为研究对象,分别测量其体重、体长、体宽和尾柄宽性状的表型值,利用简化基因组测序技术(2b-RAD)获得相应基因型数据,进行全基因组关联研究(Genome-wide association study, GWAS),筛选与大菱鲆体重和体尺性状显著关联的数量性状核苷酸(Quantitative trait nucleotides, QTNs)遗传位点。结果显示,以多性状线性混合模型(mv LMM)对体重–体长和体长–体宽–尾柄宽2个性状组合进行多性状GWAS分析,分别检测到9个和2个一因多效QTNs;以单一性状线性混合模型(LMM)对各个性状进行GWAS分析,在体重性状中检测到4个与之显著关联的QTNs,在体长和体宽性状中各检测到1个QTN,而在尾柄宽性状中则没有检测到显著的遗传位点。比较2种模型的结果,发现mvLMM相较于LMM能够检测到更多QTNs,且检测到的QTNs为更具生物学意义的一因多效QTNs。本研究首次利用mvLMM和LMM对大菱鲆体重和体尺性状进行联合GWAS分析,共筛选到17个显著的QTNs,其中,有4个QTNs被重复检测到。以这些检测到的QTNs为探针,在大菱鲆全基因组上找到了距离其最近的12个候选基因,它们可能是影响大菱鲆体重和体尺性状的重要候选标记和功能基因,本研究为大菱鲆体重和体尺性状的分子标记辅助选育提供了理论素材和参考。 相似文献
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大口黑鲈为肉食性鱼类,传统养殖过程中使用大量的冰鲜鱼为饵料,冰鲜鱼的使用不仅增加了养殖成本,而且多余的冰鲜鱼残饵还会给环境带来严重的污染。为了保护环境并降低养殖成本必须减少冰鲜鱼的使用量,本实验以选育适合人工配合饲料饲喂大口黑鲈为研究目标,用人工配合饲料作为饵料驯化大口黑鲈幼鱼,在驯食后14和16 d,测定其体质量、全长、体高等生长指标。同时在大口黑鲈转录组测序分析获得的SNP位点中,选择其所在序列的基因功能与能量代谢有相关性的7个SNP位点进行SNaPshot分型,用SPSS 19.0进行卡方分析标记与生长性状的相关性。结果发现,序列Unigene031044中的C1332G位点、Unigene085384中的A741G位点和Unigene022319中的A284G位点在体质量、全长及体高性状上存在显著差异。这3个SNP位点所在的基因经预测分别与雌二醇17β脱氢酶12B (hsd17b12b)基因、长链酰基辅酶A合成酶家族成员1(acsl1)基因和琥珀酸脱氢酶组装因子2(sdhaf2)基因具有很高的同源性,推测上述3个SNP位点的变异可能影响了基因表达产物对脂肪酸代谢或三羧酸循环的调节功能,与大口黑鲈对人工配合饲料的利用能力存在密切的相关性。本研究筛选得到的与驯食相关的SNP位点为大口黑鲈功能基因多态性的进一步研究,及加快大口黑鲈食性改良与驯化育种的遗传研究提供了科学的参考依据。 相似文献
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为了解草鱼GH基因多态性与早期生长性状及肌肉成分的相关性,实验利用直接测序法从156尾草鱼GH基因的3′部分序列中共筛选到9个变异位点(分别命名为SNP1~SNP9:G2825A、G2914T、T2966G、A3002T、T3022C、A3301G、C3463T、C3547T、C3620T)。卡方检验结果显示,9个位点均未显著偏离Hardy-Weinberg平衡,且均表现为中等多态性(0.25PIC0.5);经连锁不平衡分析发现,SNP3、SNP4和SNP5位点为一组完美连锁不平衡,SNP2、SNP7、SNP9位点为一组完美连锁不平衡;GH基因3′部分序列5个位点单倍型分析共发现6种单倍型,其中Hap1(30.4%)所占的比例最高,Hap6(4.8%)所占比例最低。利用GLM及多重比较对草鱼GH基因中9个SNPs多态性与早期生长性状和肌肉成分进行相关性分析,发现SNP2和SNP3位点的纯合突变型在体质量、体长和粗脂肪性状上均显著高于野生型和杂合突变型;在双倍型分析时得出了相似的结果,同时含有SNP2和SNP3位点纯合突变的双倍型组合在生长和粗脂肪性状上均显著性高于其他组合。研究表明,草鱼GH基因中SNP2和SNP3位点与早期生长性状及肌肉成分存在显著性相关,可作为草鱼生长及肉质改良的候选辅助分子标记,并且为进一步对相关变异位点的功能验证奠定研究基础。 相似文献
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为研究牙鲆(Paralichthys olivaceus)肌肉生长抑制基因(mstn)多态性,开发其分子辅助育种新标记,采用重测序方法对120尾牙鲆(3个双克隆杂交家系：60尾;3个雌核发育家系：60尾)的mstn基因进行单核苷酸多态性(Single nucleotide polymorphism, SNP)位点筛选,并对不同基因型个体的生长性状进行多重比较及SNP标记验证。结果显示,共检测到7个SNPs位点,均为颠换型突变;外显子区3个(Exonl 1:G2063A; Exonl 3:C3883T, C4009A),为同义突变;内含子区3个(Intron 1:A2444T; Intron 2:T3816C, A3832C);3’端非编码区1个(3’UTR:C4564A)。SNPs与生长性状关联分析结果表明,A3832C和C4564A标记位点与牙鲆体质量、体长、体高等生长性状显著相关(P<0.05),其中A3832C位点AA基因型和C4564A位点AC基因型在生长上表现出优势,A3832C位点CC和C4564A位点AA、CC基因型在生长上表现出劣势,其他5个SNPs位点对牙鲆生长... 相似文献
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在动物体内组织蛋白酶B(cathepsin B,CTSB)是以酶原形式存在于溶酶体中,能水解多种蛋白,是调控动物生长发育的重要候选功能基因。为开发与大口黑鲈(Micropterus salmoides)生长性状相关的单核苷酸多态性(single nucleotide polymorphism,SNP)标记,本研究对大口黑鲈EST-SNP库中CTSB基因上的SNP位点进行筛选。经PCR扩增和测序分析,从该基因中筛选到1个SNP位点(命名为C+598G),该位点属于错义突变位点,编码的甘氨酸突变成为精氨酸。采用Sna Pshot方法对C+598G位点在165尾大口黑鲈养殖样本中的基因型进行检测,并分析其在群体中的遗传多样性。结果显示,C+598G位点在养殖群体中存在CC、GC和GG3种基因型,其频率分别是40.6%、47.3%和12.1%,C和G等位基因频率分别是64.2%和35.8%;该位点在群体中的观测杂合度(Ho)和多态信息含量(PIC)分别为0.473和0.354,符合哈代-温伯格平衡定律。进一步分析不同基因型与大口黑鲈生长性状相关性,结果表明,3种基因型个体的体质量、全长、体高、尾柄长和尾柄高性状平均值从小到大依次为GG型、GC型和CC型,其中GG型个体在体质量上显著小于CC型个体(P<0.05)。本研究获得的C+598G标记与大口黑鲈生长性状显著相关,可作为大口黑鲈分子标记辅助育种研究中的生长标记。 相似文献
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探究了工厂化、网箱养殖模式对黄条鰤(Seriola lalandi)幼鱼生长性能及消化道菌群的影响, 通过 16S rRNA 高通量测序和生物信息学方法, 分析了两种养殖模式下黄条鰤幼鱼消化道(胃、幽门盲囊、肠道)、饵料及养殖水中的菌群结构特征及其互作关系。结果显示, 本实验条件下, 网箱养殖黄条鰤幼鱼较工厂化养殖鱼生长性能显著提升; 在黄条鰤幼鱼消化道菌群方面, 门水平上的拟杆菌门(Bacteroidetes)、厚壁菌门(Firmicutes)和属水平上的拟普雷沃氏菌属(Alloprevotella)、拟杆菌属(Bacteroides)在网箱养殖模式中其丰度值高于工厂化养殖模式, 其中拟杆菌属仅出现于网箱养殖模式且呈现显著性差异; Beta 多样性分析显示消化道菌群更多受到饵料菌群的影响, 受水环境影响相对较小。KEGG 注释分析表明两种养殖模式的差异菌群中, 网箱养殖幼鱼消化道菌群主要参与磷酸转移酶系统 (PTS)和 NOD 样受体信号通路, 而工厂化养殖幼鱼为碳水化合物代谢和类胡萝卜素合成通路。本研究表明, 环境菌群中, 饵料菌群对消化道菌群的影响大于养殖水体菌群; 消化道微生物群落通过调整其组成结构从而改变菌群功能通路的方式, 积极参与两种养殖模式下黄条鰤幼鱼生长机能差异的调控。因此, 网箱养殖鱼表现出更快速的生长性能可能是由于机体内的菌群产生了更多的短链脂肪酸并诱导 IGF-1 等生长相关功能基因的表达, 从而促进机体的营养吸收和生长。本研究预期结果将为黄条鰤专用配合饲料的研制和健康养殖技术开发提供微生态理论支撑。 相似文献
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The performance of larval Seriola lalandi (Valenciennes, 1833) is affected by the taurine content of the Artemia on which they are fed 
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下载免费PDF全文 This study describes the effects of feeding taurine‐supplemented Artemia on the growth, survival, whole body taurine content and jaw malformation rate of larval yellowtail kingfish Seriola lalandi. Larvae were fed rotifers containing no supplemental taurine from 3 to 15 day post hatch (dph) and Artemia co‐enriched with taurine from 12 to 22 dph. Artemia were supplemented at concentrations of either 0, 0.8, 1.6, 2.4, 3.2 or 4.0 g of taurine L?1 during the 18 h HUFA enrichment process. Taurine content in the Artemia increased from 0.76 ± 0.04% DW in those without supplementation to 3.95 ± 0.17% DW in those supplemented at 4.0 g L?1. Survival rates of larval yellowtail kingfish were significantly lower in all taurine‐supplemented treatments compared to the unsupplemented control. Growth was significantly improved in those larvae fed taurine‐supplemented Artemia; however, we cannot attribute this improvement solely to taurine, as improved growth may have been a function of the reduced survival, and therefore increased prey availability, in these treatments. The whole body taurine content of larvae fed unsupplemented Artemia was significantly lower (1.85 ± 0.03% DW) than those fed supplemented Artemia, which did not differ from each other (pooled average 2.48 ± 0.03% DW), suggesting either a functional excretion mechanism is in place or that this represents the saturation value for larvae of this age. Jaw malformation rates were not affected by Artemia taurine content. The results of this research suggest yellowtail kingfish larvae may have a lower requirement and/or a reduced tolerance to excess dietary taurine than juveniles. 相似文献
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Jenna N Bowyer Mark A Booth Jian G Qin Trent D'Antignana Michael J S Thomson David A J Stone 《Aquaculture Research》2014,45(12):2010-2020
A 5 week experiment was carried out with juvenile yellowtail kingfish Seriola lalandi to investigate the interactive effects of water temperature (21, 24, or 27°C) and dissolved oxygen regime (normoxic vs. hypoxic) on the growth rate, feed intake and digestive enzyme activity of this species. Specific growth rate (SGR) was highest at 24°C, regardless of oxygen regime, but the SGRs of the fish exposed to hypoxia at 21, 24 and 27°C were 13%, 20% and 17% lower, respectively, than the SGRs recorded for the fish reared under normoxic conditions. The digestive enzyme activities (i.e. trypsin, lipase and α‐amylase) were influenced by temperature but did not appear to be affected by dissolved oxygen concentration. Information about the effects of water temperature and dissolved oxygen on feeding, growth and digestive capacity of juvenile yellowtail kingfish could contribute to improving feed management decisions for production of this fish species under different environmental conditions. 相似文献
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Quantitative genetic properties of four measures of deformity in yellowtail kingfish Seriola lalandi Valenciennes, 1833 下载免费PDF全文
下载免费PDF全文 The main aim of this study was to estimate the heritability for four measures of deformity and their genetic associations with growth (body weight and length), carcass (fillet weight and yield) and flesh‐quality (fillet fat content) traits in yellowtail kingfish Seriola lalandi. The observed major deformities included lower jaw, nasal erosion, deformed operculum and skinny fish on 480 individuals from 22 families at Clean Seas Tuna Ltd. They were typically recorded as binary traits (presence or absence) and were analysed separately by both threshold generalized models and standard animal mixed models. Consistency of the models was evaluated by calculating simple Pearson correlation of breeding values of full‐sib families for jaw deformity. Genetic and phenotypic correlations among traits were estimated using a multitrait linear mixed model in ASReml. Both threshold and linear mixed model analysis showed that there is additive genetic variation in the four measures of deformity, with the estimates of heritability obtained from the former (threshold) models on liability scale ranging from 0.14 to 0.66 (SE 0.32–0.56) and from the latter (linear animal and sire) models on original (observed) scale, 0.01–0.23 (SE 0.03–0.16). When the estimates on the underlying liability were transformed to the observed scale (0, 1), they were generally consistent between threshold and linear mixed models. Phenotypic correlations among deformity traits were weak (close to zero). The genetic correlations among deformity traits were not significantly different from zero. Body weight and fillet carcass showed significant positive genetic correlations with jaw deformity (0.75 and 0.95, respectively). Genetic correlation between body weight and operculum was negative (?0.51, P < 0.05). The genetic correlations' estimates of body and carcass traits with other deformity were not significant due to their relatively high standard errors. Our results showed that there are prospects for genetic selection to improve deformity in yellowtail kingfish and that measures of deformity should be included in the recording scheme, breeding objectives and selection index in practical selective breeding programmes due to the antagonistic genetic correlations of deformed jaws with body and carcass performance. 相似文献
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齐口裂腹鱼(Schizothorax prenanti)是中国特有的冷水性经济鱼类,具有较高的营养和经济价值。为了研究齐口裂腹鱼生长性状关联的分子标记,本研究以114尾同批次繁殖、相同养殖条件的齐口裂腹鱼为研究材料,运用26个齐口裂腹鱼SNPs位点进行生长性状(体重、体高、全长、体长)关联分析。对齐口裂腹鱼生长性状的主成分分析表明,体重占解释方差的93.42%,且特征根大于1,累积方差大于85%,是齐口裂腹鱼生长性状的第一主成分。SNPs位点与生长性状相关分析结果显示:ug17711-0-2201与全长呈显著相关(P0.05),ug24013-0-3669对全长、体长有显著影响(P0.05),ug25050-0-1678对体重、体高、全长和体长都有显著影响(P0.05),ug22712-0-2452对体重、体高、全长和体长都有极显著影响(P0.01)。对与生长性状显著相关的4个SNPs位点进行多态性检测,平均观测杂合度和期望杂合度分别为0.2369和0.2110,平均多态信息含量为0.18,其中ug25050-0-1678具有中度多态性(0.25≤PIC0.5)。综上所述,ug25050-0-1678和ug22712-0-2452与生长性状具有显著相关性,可作为候选位点用于齐口裂腹鱼的分子标记辅助育种。本研究旨为齐口裂腹鱼的遗传改良和选择育种提供基础资料。 相似文献
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Ky T. Le Thuy T. T. Dao Ravi Fotedar Gavin J. Partrigde 《Aquaculture International》2014,22(2):435-446
The effects of dietary selenium (Se) and vitamin E and their interaction in the nutrition of yellowtail kingfish, Seriola lalandi, were investigated. Six dietary treatments were prepared in a 3 × 2 factorial arrangement (not supplemented or supplemented with Se at 1 or 2 mg kg?1 × supplemented with vitamin E at 40 or 180 mg kg?1). A group of fish in triplicate were fed one of the six experimental diets for 6 weeks, and their growth performance, haematological and immune responses were measured. The results revealed positively interactive effects between dietary Se and vitamin E in yellowtail kingfish. Se significantly increased weight gain of fish fed diets low in vitamin E, but not high in vitamin E. Simultaneous supplementation of both micronutrients resulted in significant increase in serum bactericidal activity. There was no significant effect of Se or vitamin E on survival, feed intake, feed conversion ratio, haematocrit, white blood cell counts and fillet proximate composition. However, Se and vitamin E contents in fillets were significantly responsive to dietary Se and vitamin E, respectively. The supplemental level of Se at 2 mg kg?1 significantly increased red blood cell counts and haemoglobin concentrations, while lysozyme activity in skin mucus was significantly stimulated by vitamin E. The findings of Se and vitamin E supplementation in this study can be applied to improve growth and health indices of yellowtail kingfish. 相似文献