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齐口裂腹鱼（Sclizothorax）隶属鲤科，裂腹鱼亚科，俗称雅鱼，又称洋鱼、细甲鱼等，自然分布于中国长江上游，金沙江、岷江、大渡河、青衣江及乌江下游等水域，是产区的名贵鱼类，也是我国特有的重要冷水性经济鱼类。现在已经有关于齐口裂腹鱼人工繁殖取得成功和养殖的少量报道， 相似文献
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齐口裂腹鱼人工养殖技术 总被引：1，自引：0，他引：1
齐口裂腹鱼（Schizothorax prenanti）,俗称雅鱼、齐口、细甲鱼、齐口细鳞鱼,为四川省级保护动物,是我国特有的重要名优经济鱼类。由于其品质优良,肉嫩鲜美,营养丰富,颇受广大消费者喜爱。目前,齐口裂腹鱼作为一种新的名优养殖鱼类,其引种驯化、规模化养殖与人工繁殖越来越受到广大水产科技人员及养殖者的重视。本文就齐口裂腹鱼人工养殖技术进行阐述,以期为齐口裂腹鱼的驯养提供依据,促进该鱼产业化养殖的发展。 相似文献
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齐口裂腹鱼是我国特有的亚冷水性经济鱼类,也是长江上游增殖放流的重要品种。为探究齐口裂腹鱼热休克蛋白60(Schizothorax prenanti Heat shock protein 60, SpHsp60) cDNA基因序列的分子特征和在细菌感染中的响应情况。本研究利用RACE技术克隆获得了2277 bp的SpHsp60 cDNA序列,预测编码575个氨基酸,其与脊椎动物具有较高的保守性;齐口裂腹鱼的组织表达模式分析显示,SpHsp60在肝胰脏表达量最高,血液次之,而在皮肤、肌肉和肠道中表达量低;无乳链球菌感染过程中,血液、肝胰脏、中肾和脾脏的SpHsp60 mRNA表达量在感染后6 h发生显著性变化(P<0.05),提示组织中SpHsp60能快速响应感染;且肝胰脏和中肾在感染后6 h-72 h表达量变化均显著高于对照组,而脾脏则显著低于对照组(P<0.05)。研究表明SpHsp60可能通过快速响应参与齐口裂腹鱼对抗细菌感染的过程,研究为肝胰脏和中肾组织中Hsp60 mRNA表达量作为齐口裂腹鱼感染无乳链球菌的危险信号分子提供基础数据。 相似文献
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鱼类细胞培养是进行种质保存、基因功能分析、细胞工程育种等研究的重要材料和模型。为了建立濒危的宽口裂腹鱼(Schizothorax eurystomus)中肾组织细胞系,本研究以其中肾组织为材料,以组织块移植法启动细胞原代培养,待细胞稳定后再进行传代培养,建立宽口裂腹鱼中肾组织细胞系,命名为EUM10。分析宽口裂腹鱼中肾细胞系冻存复苏能力和最适生长条件,且通过PCR技术检验污染状况,用线粒体基因片段分析鉴定细胞的来源。结果显示,宽口裂腹鱼中肾组织细胞的最佳培养基为DME/F-12,最适温度为25℃,最适血清浓度为20%;细胞呈悬浮生长,生长曲线为“S”型曲线,第10代中肾组织细胞系细胞的群体倍增时间为23.26 h;第6代细胞液氮冷冻保存6个月后,复苏存活率达91.91%;经污染鉴定无污染现象;EUM10第10代细胞线粒体16S rRNA序列分析结果与宽口裂腹鱼基因序列一致性为99.81%,表明细胞系来自宽口裂腹鱼。本研究成功建立了宽口裂腹鱼中肾组织细胞系,可将宽口裂腹鱼中肾细胞系作为体外体系运用于宽口裂腹鱼的研究,如细胞遗传学、基因功能分析等,可为宽口裂腹鱼的基础研究和育种工作提供基础资料。 相似文献
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齐口裂腹鱼[Schizothorax (S.)prenanti(Tchang)](图见彩中插2),属鲤科、裂腹鱼亚科、裂腹鱼属鱼类,别名雅鱼、细鳞鱼、鲤鱼。随着市场对齐口裂腹鱼需求量的增加,其天然资源量供不应求,为满足市场对齐口裂腹鱼的需要,四川、重庆一些地区已开展流水池塘和网箱养殖。齐口裂腹鱼的养殖近几年发展比较快, 相似文献
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齐口裂腹鱼人工养殖的生物学研究 总被引：1，自引：0，他引：1
齐口裂腹鱼为四川有名的珍贵鱼类,属鲤科、裂腹鱼亚科、裂腹鱼属,其肉质细嫩、肉味鲜美,氨基酸含量较高,每克干物质赖氨酸含量高达82.9mg,为目前经检测含量最高的鱼之一,被称为雅鱼,其市场价格高达120元/kg左右,已成为一个新的养殖对象。为推进齐口裂腹鱼人工养殖的发展,现将我们在养殖中掌握的生物学特征简介如下,供参考。 相似文献
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正细鳞裂腹鱼(Schizothorax chongi)隶属鲤科(Cyprinidae)、裂腹鱼亚科(Schizothoracinae)、裂腹鱼亚属(Schizothorax Heckel),俗称细甲鱼,主要分布在长江上游、金沙江下游、雅砻江下游等江段,是一种重要的经济鱼类。近年来,因产区水电资源开发,细鳞裂腹鱼的资源保护开始引起国内鱼类生态学者的关注。目前在细鳞裂腹鱼的生殖生物学、人工繁殖、游泳行为学等方面已开展了研究。在人工繁 相似文献
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齐口裂腹鱼的生物学特性与资源保护 总被引：1，自引：0，他引：1
齐口裂腹鱼地方名颇多，如：齐口、细甲鱼、雅鱼、丙穴鱼等，属鲤形目，鲤科，裂腹鱼亚科，裂腹鱼属。分布于长江、金沙江、岷江、大渡河、青衣江、西水、汉江的上游，主产区为四川雅安地区。齐口裂腹鱼为口下位，下颌前缘具发达锐利的角质，以在岩石、泥土上刮取食物。齐口裂腹鱼肉肥质嫩，富含脂肪，味道鲜美，具广阔的市场养殖前景。１生物学特性１．１生态习性齐口裂腹鱼是底栖鱼类，喜生活于山区河弯急流处。据实验，齐口裂腹鱼生存水温为０．８～３３．５℃，适宜生长水温为５～２７℃。窒息点为０．５８毫克／升，溶氧１．２８毫克／… 相似文献
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Shun Watanabe Jun Aoyama Seishi Hagihara Bunpei Ai Rhodora V. Azanza Katsumi Tsukamoto 《Fisheries Science》2013,79(3):375-383
Anguilla luzonensis and A. huangi were each described in 2009 using eels obtained from northern Luzon Island. We examined the taxonomic status of these two groups of eels using morphological and molecular genetic characters. There were no significant differences in two vertebrae counts between eels of A. luzonensis and A. huangi. Mitochondrial 16S ribosomal RNA and cytochrome b genes sequences were obtained and compared among 28 specimens of A. luzonensis, the holotypes of A. luzonensis and A. huangi, and one specimen of the other 15 anguillid species. The specimens of A. luzonensis exhibited almost identical sequences, including the holotype, with only a few site differences, and the genetic difference between the holotypes of A. luzonensis and A. huangi was within the range of differences of specimens of A. luzonensis. The other anguillid species were genetically very different from A. luzonensis and A. huangi, although A. interioris is a closely related species. It is clear that A. luzonensis and A. huangi are the same species, and according to the principle of priority in zoological nomenclature, A. luzonensis Watanabe, Aoyama, and Tsukamoto, 2009 is the valid species name, and A. huangi Teng, Lin, and Tzeng, 2009 is a junior synonym of A. luzonensis. 相似文献
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Nuhman Usman Bambang Irawan Agoes Soegianto 《Journal of the World Aquaculture Society》2013,44(4):547-556
The purpose of this study was to evaluate the acute toxicity of lead for different life stages of shrimp, Litopenaeus vannamei, and to determine the effect of its sublethal concentrations on osmoregulatory capacity (OC) as well as the possible histological alteration in the gills of juvenile shrimp. The 24‐, 48‐, and 96‐h LC50 values for lead to L. vannamei increased progressively with increasing life stage, from nauplii < zoeae < mysis < postlarvae < juvenile. After 15‐d exposure to 3.25, 6.50, and 9.75 mg Pb/L, the OC values of exposed shrimp were reduced by 39, 73, and 157%, respectively compared to control animals. Compared to controls, the lead concentrations in gill tissues increased significantly by 127,500, 137,500, and 141,000% in shrimps exposed to 3.25, 6.50, and 9.75 mg Pb/L, respectively. After lead exposure, hemocytic congestion in efferent vessels and multiple hyperplasia were observed in gill filaments, resulting in narrowed hemolymphatic lacuna. The histopathological effects increased with increasing Pb concentration. 相似文献
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Charles C. Mischke Matt J. Griffin Terrence E. Greenway David Wise 《Journal of the World Aquaculture Society》2013,44(2):288-292
Mosquitofish, Gambusia sp., have been spread throughout the world to biologically control mosquitoes. However, the fish has gained a reputation as an invasive species and has been implicated in displacing native aquatic species. Gambusia affinis are native to the southeastern United States and commonly occur in commercial channel catfish, Ictalurus punctatus, production ponds. We investigated effects of mosquitofish presence on zooplankton populations, water quality, disease occurrence, and fish production in experimental ponds. There were no differences between ponds with or without mosquitofish in numbers of calanoid copepods, cyclopoid copepods, total copepods, Bosmina sp., Ceriodaphnia sp., Moina sp., Daphnia sp., or total cladocerans. There were also no differences in copepod and cladoceran sizes. Copepod nauplii were more numerous during the summer months in ponds with mosquitofish. There were no differences in water quality variables (soluble reactive phosphorus, nitrate, nitrite, ammonia, total nitrogen, total phosphorus, pH) or phytoplankton density between ponds stocked with and without mosquitofish. Catfish production and disease occurrence were also similar between ponds with and without mosquitofish. Although mosquitofish may cause problems when stocked outside their native range, there does not appear to be any adverse effects of mosquitofish presence in catfish production ponds. 相似文献
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Mulloway (Argyrosomus japonicus) occur in estuarine and coastal waters surrounding Australia, Africa, India, Pakistan, China, Korea and Japan, where they are important in fisheries. This study identified that mulloway in south-eastern Australia had similar growth rates, but matured at smaller lengths and younger ages, to those in South Africa and Western Australia. Growth of both sexes was similar to about 5 years, after which females grew faster and attained a greater maximum length than males. Female mulloway matured at 4–5 years of age with a L50 of 68 cm, whereas males matured at 2–3 years of age with a L50 of 51 cm. The commercial fishery in New South Wales was characterised by declining catches and a reduction in the proportion of mulloway of mature lengths in landings. During 2002–2005 commercial landings were dominated (83%) by fish within 15 cm of the current minimum legal total length of 45 cm and aged 2 and 3 years (>80%), even though mulloway can attain lengths of 200 cm and live >30 years. Estimates of the rates of instantaneous total mortality ranged between 0.34 and 0.45, whilst the rate of instantaneous natural mortality (M) was estimated to be approximately 0.12. Yield-per-recruit analyses indicated that mulloway in New South Wales are being growth overfished and substantial increases in yield could be achieved by increasing the length at first harvest. Values of the spawning potential ratio were below 0.2 under a range of mortality estimates, suggesting that mulloway are at risk of recruitment overfishing. These results suggest that the spawning stock of mulloway in south-eastern Australia has been depleted and that remedial management action is required to protect this iconic species. 相似文献
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The effects of exposing the eggs of Pacific threadfin and amberjack eggs (AEs) to different concentrations of hydrogen peroxide for 5 min on hatch rate and survival were assessed in a series of experiments using a petri dish model rearing system. Despite significant inter‐batch variation in hatch rate, it was shown that eggs of both species could be safely exposed to up to 11 340 mg L−1 H2O2 for 5 min. Exposure to 34 230 mg L−1 H2O2 for 5 min was shown to be lethal to AEs at a late stage of development. In two further experiments, it was demonstrated that Pacific threadfin eggs were resistant to all tested concentrations of a range of polyvinylpyrrolidone iodine (PVP‐I) concentrations and contact times (up to 1000 mg L−1 PVP‐I for 10 min). The level of bacteria adhering to the eggs of both species was highly variable. Where eggs were heavily colonized (>104 cfu egg−1), hydrogen peroxide concentrations of at least 11 340 mg L−1, or PVP‐I concentrations higher than 500 mg L−1 for 10 min, were required for effective sterilization. In less colonized batches, rinsing in sterile seawater or exposure to lower (550 mg L−1) concentrations of H2O2 was sufficient to result in high apparent levels of surface sterility (<1 cfu egg−1). 相似文献
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《水生生物资源》1999,12(4):239-246
The effect of different parameters on short-term storage capacity of turbot ova was assessed over a 45-h period after ova collection for fertilization rates and over a 9-h period after ova collection for hatching rates. Increasing the volume of ova sampling from 0.5 to 2.5 mL, as well as adding an antibiotic–antimicotic solution or oxygen did not significantly change the storage capacity of ova. Regarding the hatching rates, a higher storage ability was recorded at 8 and 13 °C, compared to 3 °C. The mean composition of the ovarian fluid was determined (n = 57 spawns). Use of a diluent mimicking the ovarian fluid significantly decreased the storage ability as assessed by the fertilization rates but did not modify the hatching rates. Diluting ova in an artificial ovarian fluid deprived of calcium significantly decreased the fertilization and hatching rates during the storage period. Furthermore, addition or not of soybean trypsin inhibitor (Sigma T 9003) to the artificial ovarian fluid deprived of calcium did not significantly change the results. Storage capacity of control batches of ova was low: at 13 °C, without any diluent and when ova were fertilized 3 h after stripping, the hatching rate was lowered to 62.4 ± 29.4 % (mean ± SD) of the initial value. 相似文献
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Effects of Dietary Savory and Myrtle Essential Oils on Growth,Survival, Nutritional Indices,Serum Biochemistry,and Hematology of Farmed Rainbow Trout,Oncorhynchus mykiss,Fry 
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下载免费PDF全文 Mohsen Mohamadi Saei Kiarash Beiranvand Mohammad K. Khalesi Farzaneh Mehrabi 《Journal of the World Aquaculture Society》2016,47(6):779-785
The effects of diets containing different levels of essential oils from savory Satureja khuzestanica Jamzad and myrtle Myrtus communis L and an immunity complement were investigated on growth, survival, nutritional indices, serum biochemistry, and hematology of farmed rainbow trout, Oncorhynchus mykiss, fry (n = 4500; 5 ± 2 g). The essential oils were dosed as 300 and 500 mg/kg diet. After 60 d, the fish fed 300 mg/kg of the essential oils showed the highest counts of white blood cells (P < 0.05). The same treatment revealed the greatest levels of hematocrit, total protein, and albumin, but glucose and cholesterol values significantly decreased compared with the control (P < 0.05). Feeding fish with 300 mg/kg of the essential oils led to highest records of specific growth rate and survival rate but the lowest feed conversion ratio (FCR), and those fed with immunity complement attained the lowest values of growth parameters. The fry fed with immunity complement and 300 mg/kg of myrtle essential oil achieved the highest and lowest FCR values, respectively. The results indicate that dietary supplementation of 300 mg/kg of the above essential oils exert positive impacts on the growth performance, feeding efficiency, and hematological factors in O. mykiss. 相似文献
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Sperm Motilities in Waigieu Seaperch,Psammoperca waigiensis: Effects of Various Dilutions,pH, Temperature,Osmolality, and Cations 下载免费PDF全文
下载免费PDF全文 The objectives of this study were to evaluate the effects of varying dilutions, pH, temperature, osmolality, and cations on sperm motility parameters in waigieu seaperch, Psammoperca waigiensis. The maximum velocity of average in path (VAP), percentage of motile cells (MOT), and duration of sperm motility (DSM) were observed when semen was diluted in artificial seawater (ASW) at a ratio of 1:100 (144.9 ± 0.6 µm/sec, 95.6 ± 0.4%, and 230.3 ± 2.3 sec, respectively), at 30 C (142.0 ± 0.6 µm/sec, 93.6 ± 0.4%, and 238.3 ± 0.9 sec, respectively), and pH 8 (144.8 ± 0.6 µm/sec, 93.3 ± 0.4%, and 234.0 ± 1.5 sec, respectively). Maximum VAP, MOT, and DSM were obtained in each solution containing 0.6 M NaCl (143.8 ± 1.0 µm/sec, 91.3 ± 2.0%, and 230.6 ± 4.2 sec, respectively), 0.6 M KCl (135.1 ± 3.1 µm/sec, 91.1 ± 3.1%, and 230.3 ± 3.7sec, respectively), 0.2M CaCl2 (105.3 ± 4.7μm/sec, 47.9 ± 2.7%, and 120.7 ± 1.3 sec, respectively), 0.2 M MgCl2 (107.3 ± 3.0 m/s, 42.1 ± 3.3%, and 120.3 ± 4.8 sec, respectively), and osmolality of 400 mOsm/kg (145.1 ± 2.5 µm/sec, 93.0 ± 2.1%, and 346.5 ± 4.4s, respectively). We used these mediums as artificial insemination media for fertilizing matured eggs. The results showed that the fertilization and hatching rates in 0.6 M NaCl (75.3 ± 0.6% and 57.0 ± 2.4%, respectively), ASW (70.8 ± 1.2% and 51.2 ± 1.8%, respectively), or 400 mOsm/kg (72.9 ± 1.8% and 55.3 ± 1.6%, respectively) were higher than that in seawater (63.9 ± 1.2% and 39.2 ± 3.9%, respectively). In conclusion, using 0.6 M NaCl, ASW, or 400 mOsm/kg as an artificial insemination medium is effective for fertilizing of waigieu seaperch. 相似文献
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对驼背鲈(Chromileptes altivelis)的胚胎发育及仔、稚、幼鱼的形态特征进行了详细的观察与研究,描述了从受精卵到仔、稚、幼鱼各发育期的时间和形态特征变化。结果表明,在水温25-26℃、盐度30的海水中,受精卵历时27 h 25 min完成整个胚胎发育过程,经历从卵裂、囊胚、原肠、神经胚到肌节形成、各器官的逐渐形成、变化、完善等一系列的胚胎发育和变化过程;根据其卵黄囊消长情况、鳞片的覆盖状态、体色发生的不同变化以及第二背鳍和腹鳍的消长,将胚后发育分为仔、稚、幼鱼3个阶段。在水温22-26℃、盐度29-31、DO≥5 mg/L的条件下,2-3 d仔鱼卵黄囊消失,开口摄食;生长发育至31 d,仔鱼已变态进入稚鱼期;培育至57 d,稚鱼完成变态,成为幼鱼。第二背鳍棘和腹鳍棘的生长与收缩等石斑鱼类早期发育的共性生长特征及其体表特性体色变化特征为驼背鲈胚后发育过程中最明显的特征。 相似文献
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Recruitment strategy of swimming crab, Portunus trituberculatus, in Hiuchi-Nada, Japan 总被引：2，自引：0，他引：2
The recruitment strategy of swimming crabs, Portunus trituberculatus, was investigated by field observations and numerical experiments in Hiuchi-Nada, Japan. Calculated dispersal patterns of zoeal and megalopal stages in a three-dimensional numerical model reproduce observed patterns accurately. The temporal change of vertical migration patterns between the 1st and 2nd zoeas and the 3rd and 4th zoeas plays an important role in the process of recruitment of P. trituberculatus from spawning ground to nursery ground. 相似文献