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1.
One of the most important periods in artificial breeding of sturgeon is incubation. In general, little is known about ontogeny of the stress response in early developmental stages of sturgeon. In this study, cortisol content was measured by radioimmunoassay for the first time in eggs of Persian sturgeon Acipenser persicus to elicit at which times during early developmental stages, that they were particularly sensitive to stressful events. In this study, fertilized eggs of mature Persian sturgeons were placed in special boxes (as replicates) that were put in incubation units of Yushchenko. To assess pre-stress samples (resting cortisol levels), 5 eggs were selected randomly from each box in 7 important stages of incubation (2-cell division, blastula, end of blastula, mid of gastrula, neurula, eyed stage and heart beaten). In another experiment, eggs were exposed to an acute stress (10 min out of water) in 7 developmental stages. For determination of post-stress cortisol, eggs were collected 2 and 6 h after the stress. The results indicated significant difference in various stages of incubation but cortisol levels revealed no differences in whole-body levels between stressed samples (2 and 6 h post-stress) and unstressed samples (P > 0.05). The highest percentage of hatching rate for post-stressed eggs was recorded in 2-cell division stage while this percentage declined steadily in the heart beaten stage when a statistically significant difference was revealed with regard to other stages (P = 0.000). These findings suggested that sturgeon eggs were not so much sensitive in their early developmental stages and relatively resistant to stress. However, significant decrease in survival rate in heart beaten stage showed a sensitivity of this stage to stress. Lack of an increase in cortisol after acute stress indicated that hypothalamus-pituitary-interrenal (HPI) axis for cortisol synthesis was not yet functional. However, further studies seem necessary to clarify when HPI axis is activated in this species.  相似文献   

2.
Stress has a considerable impact on welfare and productivity of fish, and blood glucose level of fish may be a factor modulating stress response. This study evaluated the effect of blood glucose level and handling on acute stress response of grass carp Ctenopharyngodon idella. Fish were intraperitoneally injected with glucose at 0, 0.2, 0.5, and 1.0 mg g?1 body mass (BM) and then were exposed to handling for 5 min. Glucose injection resulted in increase of plasma glucose level and liver glycogen content and decrease of plasma lactate level. Handling resulted in increase of plasma levels of cortisol, glucose, and lactate and plasma lactic dehydrogenase (LDH) activity and decrease of liver glycogen content. At 1 h post-stress, the plasma cortisol level was lower in the stressed fish injected with glucose at 0.5 mg g?1 BM than the stressed fish injected with glucose at 0, 0.2, and 1.0 mg g?1 BM. No significant differences were found in the activities of phosphoenolpyruvate carboxykinase (PEPCK) and pyruvate kinase (PK) in the liver between the stressed and unstressed fish, regardless of the dose of glucose injection. At 1 h post-stress, the liver glucose-6-phosphatase (G6Pase) activity was higher in the fish without glucose injection than in the fish injected with glucose. This study reveals that blood glucose level can affect stress response of grass carp by modulating cortisol release and glucose homeostasis through glycogen metabolism and gluconeogenesis in the liver.  相似文献   

3.
Juveniles of Atlantic cod, Gadus morhua L., weighing 30–50 g were subjected to short-term handling stress by exposing them to air for 3 min and immediately placing in the rearing tank for recovery. Indices for the primary stress response as well as selected immune- and metabolism-related parameters were measured in the plasma before the application of the stressor and at 1-h, 24-h and 7-day post-stress. Plasma cortisol significantly increased at 1-h post-stress then returned to the prestress levels at 24-h post-exposure. Plasma glucose and total antioxidant capacity had two peaks at 1 h and 7 days after handling stress. Alkaline phosphatase significantly increased at 24-h post-exposure, while no significant changes in myeloperoxidase activity was observed. Lysozyme and antiprotease activities were significantly downregulated at 1- and 24-h post-exposure, respectively. These findings showed that short-term handling stress in Atlantic cod juveniles resulted in temporal elevation of the primary stress response and metabolism with a corresponding decrease in its defense against bacterial pathogens.  相似文献   

4.
Great sturgeon Huso huso juveniles (sub-yearling; 30–268 g) and adults (12.2 kg) were exposed to different kinds of stressors that are commonly observed in aquaculture practices including density, severe confinement, handling, and sex identification. Blood was taken pre- and post-stress for the measurement of cortisol and glucose concentrations. Results showed that in most cases, primary (cortisol) and secondary (glucose) responses of fish to stressors significantly increased. Some different responses were observed between low and high densities as well as male and female. In many cases, stress metabolites decreased to the initial levels at 9–12 h after stress. Results revealed that responses to the stressors in sturgeon are not high as like as teleosts. We established that acute stressors cause a stress response in different size classes of great sturgeon. We characterized the resting profile for cortisol and determined the dynamics of this hormone as well as under simulated management stressors. With this information, we can start the development of management practices (such as anesthesia and appropriate density and handling) that would eliminate stressors interfering with production performance and well-being of the animal in aquaculture. To achieve this, potential stressors should be avoided and possible procedures and techniques can be used or modified to reduce the degree of stress on the fish as much as possible.  相似文献   

5.
The purpose of this study was to determine the cortisol response of spotted wolffish (Anarhichas minor) after exposure to air immersion and netting (acute stress) under a range of increasing densities (chronic stressor). In addition, the presence of a cumulative negative impact from chronic stress was assessed by the application of an additional acute stressor following rearing at various densities, and proxies of behavior were evaluated (feeding and aggression data). The stress response to air immersion and netting led to increasing plasma cortisol values at 0.5, 13, and 37 h post-stress, with the highest value achieved after 13 h. At 168 h, cortisol concentrations returned to similar pre-stress value. Changes in cortisol concentrations were relatively low after acute stress (five to six fold increases) and recovery time long lasting (>37 h). Prolonged rearing at 20, 30, and 40 kg m?2 showed a non-significant trend of increasing cortisol values with increasing density, and the cortisol response after the application of an additional acute stressor was significantly altered, i.e., higher cortisol levels were measured in unstressed (chronic stress only) compared to stressed fish (chronic stress + acute stress). Our results suggest that (1) spotted wolffish is a low responder that additionally displays a long-lasting cortisol elevation following an acute stressor; (2) fixed densities did not lead to significant differences of plasma cortisol concentration or aggression levels, although involved marked changes in daily feed intake and food conversion efficiency were observed; and (3) cortisol levels reached acute stress values after prolonged chronic stress. Although this species displays indications of being relatively resistant to handling disturbances during aquaculture practices, it is nevertheless responsive to chronic stress factors that could alter the physiological response to subsequent acute stressor.  相似文献   

6.
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7.
The ontogeny of the cortisol stress response in yellow perch (Perca flavescens) was documented by measuring resting and stress-induced whole-body cortisol levels in fish at several early development stages. In newly fertilized eggs resting cortisol levels averaged 41.0 ± 3.0 pg/embryo, and decreased to 8.0 ± 3.0 pg/embryo by day 6 where they remained constant until hatch on day 10. Acute stress challenge tests (30 sec cold shock) conducted on embryonic (days 6 and 8 post-fertilization) and larval (hatch and 2 days post-hatch) yellow perch revealed no differences in whole-body cortisol levels between stressed and unstressed individuals. In acute stress challenge tests (30 sec net confinement) conducted on post-hatch yellow perch, differences in cortisol levels between stressed (30 min post-stress) and unstressed fish were detected one week after hatching (12.3 ± 1.6 ng/g and 7.0 ± 1.3 ng/g, respectively). The magnitude of the stress response was greater in 2- to 5-week-old fish than in one-week-old fish. Changes in post-stress cortisol levels were closely associated with changes in interrenal morphology. Cumulatively, these results indicate that cortisol biosynthesis, integration and maturation of the hypothalamic-pituitary-interrenal (HPI) axis can be observed in yellow perch as early as one week after hatching. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

8.
Methods were developed to assess whole‐body immunoreactive corticosteroid concentrations (IRC) and plasma levels of cortisol in Atlantic cod subjected to several common, acute stressors. A measurable corticosteroid stress response was observed at the first sampling in whole bodies of 8‐day post‐hatch (dph) larvae. Two groups of juveniles (～5 and 30 g) were subjected to a 30 s net stressor and whole‐body IRC and plasma cortisol levels were determined. Post‐stressor IRC in smaller fish rose approximately 14‐fold, peaked at 1 h, were sustained for 3–6 h and returned to pre‐stressor levels within 24 h. Post‐stressor plasma cortisol levels in larger fish rose approximately 18‐fold, peaked at 0.5–1 h, were sustained for 1–3 h and then returned to near pre‐stressor levels after 24 h. Immunoreactive corticosteroid concentrations appeared to remain elevated longer than plasma cortisol levels suggesting that steroids other than cortisol were contributing to total immunoreactivity in homogenates. Juveniles exposed to either a grading procedure or high density transport had maximal IRC and plasma cortisol levels within 90 min which returned to pre‐stressor levels within 24–72 h. A reduction in water salinity (20 g L?1) did not moderate the corticosteroid response in juveniles. The results show that Atlantic cod respond to common, acute stressors in a manner similar to other teleosts. Whole‐body homogenates can be used to identify changes in IRC in response to acute stress in cod with the caveat that recovery IRC may differ from plasma cortisol concentrations.  相似文献   

9.
Plasma growth hormone concentrations of rainbow trout,Oncorhynchus mykiss, fasted for six weeks, were significantly (p < 0.01) higher than in comparable fed animals; in the fasted fish, the levels fell progressively following acute stress (by displacing the fish within their home aquarium), with significant differences from pre-stressed fish evident between one and thirty-two hours after application of the stressor. Plasma growth hormone concentrations also fell significantly in the fed group, but differences were evident only between two and eight hours after stressor application.Plasma cortisol concentrations in pre- and post-stressed fed and fasted fish were similar. There was a bimodal response to stressor application in both groups, with significantly higher values relative to the pre-stressed sample evident one and eight hours after disturbance, but not after two, four or thirty-two hours. The changes in plasma cortisol levels between the initial (09:00h) sample and the sample taken eight hours later resembles the diet pattern seen in trout given access to self-demand feeders.Plasma glucose concentrations in pre-stressed fed animals were higher than in pre-stressed fasted fish. This relationship was also evident between one and four hours and thirty-two hours after stressor application. The post-stress rise in plasma glucose concentration was evident between one and four hours in the fed group, and between four and eight hours in fasted fish.The diel changes in plasma growth hormone and glucose concentrations could not be attributed to normal circadian patterns, and there was no apparent correlation between changes in plasma growth hormone and cortisol concentrations. There was a significant inverse correlation between plasma glucose and growth hormone concentrations when the total data set were analyzed, but these correlations were not apparent when the treatment groups were analyzed separately.  相似文献   

10.
The existence of daily rhythms under light/dark (LD) cycles in plasma cortisol, blood glucose and locomotor and self-feeding activities, as well as their persistence (circadian nature) under constant light (LL), was investigated in Senegalese sole (Solea senegalensis). For the cortisol and glucose rhythms study, 48 soles were equally distributed in 8 tanks and exposed to a 12:12 LD cycle and natural water temperature (experiment 1). After an acclimation period, blood was sampled every 3 h until a 24-h cycle was completed. Blood glucose levels were measured immediately after sampling, while plasma cortisol was measured later by ELISA. In experiment 2, the fish were exposed to LL for 11 days, and after this period, the same sampling procedure was repeated. For the study of locomotor and self-feeding rhythms (experiment 3), two groups of sole were used: one exposed to LD and the other to LL. Each group was distributed within 3 tanks equipped with infrared photocells for the record of locomotor activity, and self-feeders for feeding behavior characterization. The results revealed a marked oscillation in cortisol concentrations during the daily cycle under LD, with a peak (35.65 ± 3.14 ng/ml) in the afternoon (15:00 h) and very low levels during the night (5.30 ± 1.09 ng/ml). This cortisol rhythm persisted under LL conditions, with lower values (mean cortisol concentration = 7.12 ± 1.11 ng/ml) and with the peak shifted by 3 h. Both rhythms were confirmed by COSINOR analysis (p < 0.05). The synchronizing role of temperature and feeding schedule, in addition to light, is also discussed. Diel rhythms of glucose were not evident in LD or LL. As to locomotor and self-feeding activity, a very marked rhythm was observed under LD, with higher activity observed during the night, with acrophases located at 2:14 and 3:37 h, respectively. The statistical significance of daily rhythms was confirmed by COSINOR analysis. Under LL, both feeding and locomotor rhythms persisted, with an endogenous period (τ) around 22.5 h. In short, our findings described for the first time the existence of circadian cortisol and behavioral circadian rhythms in flat fish. Such results revealed the importance of taking into account the time of day when assessing stress responses and evaluating physiological indicators of stress in fish.  相似文献   

11.
The corticosteroid hormone cortisol is the central mediator of the teleost stress response. Therefore, the accurate quantification of cortisol in teleost fishes is a vital tool for addressing fundamental questions about an animal’s physiological response to environmental stressors. Conventional steroid extraction methods using plasma or whole-body homogenates, however, are inefficient within an intermediate size range of fish that are too small for phlebotomy and too large for whole-body steroid extractions. To assess the potential effects of hatchery-induced stress on survival of fingerling hatchery-reared Spotted Seatrout (Cynoscion nebulosus), we developed a novel extraction procedure for measuring cortisol in intermediately sized fish (50–100 mm in length) that are not amenable to standard cortisol extraction methods. By excising a standardized portion of the caudal peduncle, this tissue extraction procedure allows for a small portion of a larger fish to be sampled for cortisol, while minimizing the potential interference from lipids that may be extracted using whole-body homogenization procedures. Assay precision was comparable to published plasma and whole-body extraction procedures, and cortisol quantification over a wide range of sample dilutions displayed parallelism versus assay standards. Intra-assay  %CV was 8.54 %, and average recovery of spiked samples was 102 %. Also, tissue cortisol levels quantified using this method increase 30 min after handling stress and are significantly correlated with blood values. We conclude that this modified cortisol extraction procedure provides an excellent alternative to plasma and whole-body extraction procedures for intermediately sized fish, and will facilitate the efficient assessment of cortisol in a variety of situations ranging from basic laboratory research to industrial and field-based environmental health applications.  相似文献   

12.
Exposure of fish to stressors leads to multiple changes in the skin epithelium. We investigated the role of the stress hormone cortisol in the control of these changes by exposure of pieces of skin from the rainbow trout Oncorhynchus mykiss with an in vitro tissue culture incubation procedure. The effects of 24 h exposure to 4 cortisol concentrations (0, 50, 500 and 1000 ng/ml) were determined. Numbers of mucous, mitotic and apoptotic cells were quantitatively assessed using immunohistochemical techniques, in situ DNA nick end labelling (TUNEL), as well as conventional light and scanning electron microscopy (SEM). The cortisol receptor blocker mifepristone was used to investigate whether the effects could be attributed to the direct action of the hormone via glucocorticoid receptors. Overall, cortisol had no effect on the mucous cell population at 24 h. Incubation with the receptor blocker reduced the number of mucous cells. Cell proliferation was stimulated by the addition of 50 and 500 ng/ml cortisol, but not at 1000 ng/ml. Incubation with the receptor blocker increased proliferation in the control group (0 ng/ml) only. An increase in apoptosis occurred at 500 and 1000 ng/ml cortisol. This increase was blocked by incubation with the receptor blocker, which resulted in lower numbers of apoptotic cells in all except the 0 ng/ml controls. SEM observations corroborated the quantitative data. The results indicated that the effects of stressors on the fish epidermis mentioned above are mediated by cortisol, with the exception of mucous cell release.  相似文献   

13.
Many studies have been made about the physio-logical effects of isolated chronic or acute stress. However, few studies have been made to assess the combination of both responses. The fish submitted to chronic stress may be subjected to an additional acute stressor. The aim of the present work was to evaluate the acute stress response in Nile tilapia Oreochromis niloticus (L.) previously subjected to chronic stress. For this, two experiments were performed. In the first experiment, the fish were subjected to chronic stress followed by an additional acute stress. In the second experiment, the fish were submitted only to an acute stress. The data showed that Nile tilapia fingerlings can adapt to chronic stress situations, and this decreases, but does not eliminate, their capacity to respond to an additional acute stressor. In both experiments, plasma cortisol levels reached a peak 1 h after administration of the acute stressor. In fish previously submitted to chronic stress, the highest concentration of plasma cortisol measured was 196 ng mL–1. This value was significantly different from the cortisol concentration obtained in the second experiment (267 ng mL–1) with non-chronically stressed fish. The data also suggest that the chronic stress response can provoke a reduction in performance and growth rates compared with non-stressed fish.  相似文献   

14.
It is important to select the most appropriate method for fish sampling in streams and rivers; the sampling efficiency as well as any negative effect of the method of fish sampling on fishes such as stress, injury, and mortality must be considered. This study aimed to investigate stress hormone responses in ayu Plecoglossus altivelis in reaction to direct current (DC) electrofishing, alternating current (AC) electrofishing, and cast netting in artificial streams. The mortality, injury rate, and catch efficiency of these catching methods were also compared. It was found that immediately after capture, fish caught using a cast net exhibited significantly higher cortisol levels than did control fish that were caught rapidly by a hand net. Cortisol levels did not differ between electroshocked fish and the controls. Time required to catch 12 fish was shorter when DC was used (20 s) than when AC was used (45 s). The time required to catch fish greatly increased when a cast net was used (840 s). Cortisol levels in DC electroshocked and control treatment groups were at resting levels 24 and 48 h after capture. However, higher cortisol levels were found in fish captured using a cast net at both these time points. Cortisol levels in AC electroshocked fish returned to lower levels at 24 h, but increased again at 48 h. Furthermore, 48 h following capture, the fish caught by AC electrofishing exhibited higher mortality (7.5 %) than those captured by other methods (0 %). Spinal injury was also detected in one of the fish in this group. Considering these findings, DC electrofishing is the most effective and least damaging method for collecting P. altivelis in streams with respect to stress, physical damage, and efficiency.  相似文献   

15.
This study examined diploid and triploid shortnose sturgeon hematology and stress physiology through the investigation of various characteristics and components of whole blood and blood plasma. Erythrocytic cellular and nuclear length and width were significantly larger in triploids than in diploids. Hematocrit was depressed in triploid sturgeon in comparison to diploids, but total blood hemoglobin content and mean erythrocytic hemoglobin concentration (MEHC) did not differ between ploidies. The mean erythrocytic hemoglobin (MEH) was elevated in proportion to the increase in erythrocyte size. Taken together, these data suggest that triploids and diploids likely have similar oxygen carrying and aerobic capacities. In response to an acute stressor of 15 min chasing, plasma cortisol and glucose levels did not differ between ploidies. Cortisol levels were significantly depressed at 2 h post-stress with an increase back to 0 h post-stress levels at 6 h into recovery, whereas glucose levels did not change during the recovery period. There was a significant interaction between ploidy and time post-stress for blood hemoglobin concentrations, with diploids demonstrating elevated hemoglobin content at 6 h post-stress. Plasma osmolality, chloride ion concentrations and lactate levels were elevated in triploids. Overall, it does not appear that sturgeon have developed an elaborate stress response and the triploid stress response appears to be slightly reduced in comparison to diploids.  相似文献   

16.
Previous studies have demonstrated that fish inhabiting polluted waterways often have an impaired stress response at the organismal level. Given the possible link between the organismal (i.e. cortisol) and cellular (i.e. heat shock proteins; hsp) stress responses, we conducted this study to examine the ability of rainbow trout to respond to a 2 h, +14 °C heat stress (HS) challenge following a 28 d, sub-chronic exposure to increased concentrations of cortisol (5 mg kg−1 b.w.), β-napthoflavone (bnf; 50 mg kg−1 b.w.), and a combination of both (mixture), through the diet (1.5% b.w. every 48 h). While control fish responded to the HS by significantly increasing components of their organismal (cortisol, glucose, and lactate) and cellular (hepatic hsp70 protein) stress responses 6 and 24 h post HS, cortisol-, bnf-, and mixture-fed fish had impaired stress responses at both levels of organization. Additionally, hepatic hsp70 levels were significantly reduced 6 h post HS in cortisol-fed fish. While bnf-fed fish had significantly higher EROD activity, cortisol potentiated EROD activity in the mixture-fed fish. Similarly, plasma cortisol concentrations in the mixture-fed fish were significantly lower relative to cortisol-fed fish. These data are the first to indicate that sub-chronically stressed fish can have impaired stress responses at both the organismal and cellular levels. These findings raise questions regarding: (a) the universal and simple applicability of biological indicators of stress in fish; (b) the possible functional relationship between these two levels of stress responses; and (c) the importance of hsps in the generalized stress response of the whole organism. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

17.
Of the many stressors in aquaculture, transportation of fish has remained poorly studied. The objective of this study was therefore to assess the effects of a (simulated) commercial transportation on stress physiology of market-size African catfish (Clarias gariepinus). Catfish weighing approximately 1.25 kg were returned to the farm after 3 h of truck-transportation, and stress-related parameters were measured for up to 72 h following return. Recovery from transportation was assessed through blood samples measuring plasma cortisol, glucose and non-esterified fatty acids (NEFA) and gill histology. Also, the number of skin lesions was compared before and after transport. Pre-transport handling and sorting elevated plasma cortisol levels compared to unhandled animals (before fasting). Plasma cortisol levels were further increased due to transportation. In control fish, plasma cortisol levels returned to baseline values within 6 h, whereas it took 48 h to reach baseline values in transported catfish. Plasma glucose and NEFA levels remained stable and were similar across all groups. Transported catfish did not, on average, have more skin lesions than the handling group, but the number of skin lesions had increased compared to unhandled animals. The macroscopic condition of the gills was similar in control, transported and unhandled catfish; however, light microscopy and immunohistochemistry revealed atypical morphology and chloride cell migration normally associated with adverse water conditions. From our data, we conclude that transportation may be considered a strong stressor to catfish that may add to other stressors and thus inflict upon the welfare of the fish.  相似文献   

18.
Three experiments were conducted in flow‐through tanks at 24, 27 and 30 C to evaluate growth, food conversion, morbidity, survival, and the cortisol stress response of surubim Pseudoplatystoma sp. at different temperatures. In Experiment 1, fish (mean initial weight, 33.3 ± 7.2 g) reared at 27 and 30 C for 60 d grew significantly faster than fish reared at 24 C (P < 0.05). Fish at the lowest temperature showed the poorest feed conversion ratio (FCR, 5:1), while fish at 27 and 30 C had FCRs of 2.5:1 and 3.5:1, respectively. Mortality and morbidity rates were inversely proportional to the temperatures tested. In Experiment 2, serum cortisol levels following an acute handling stressor peaked at 30 min after stress, returned to near‐resting levels at 1 h after stress, and completely returned to resting levels at 3 h after stress in fish at all temperatures tested. Peak serum cortisol levels were higher in fish at 30 C (321.1 ± 38.8 ng/mL) than those in fish at 27 and 24 C (143.3 ± 37.4 ng/mL and 104.2 ± 37.2 ng/mL, respectively). In Experiment 3, fish (mean initial weight, 67 ± 8.58 g) reared at 27 C for 137 d grew significantly faster than fish at 30 and 24 C. Virtually, all fish reared in 30 C had some sign of morbidity (skin lesions and fin abrasion) compared to only a small percentage of fish in the other two temperatures. Again, the highest cortisol peak level was measured in fish reared at 30 C (117.6 ± 16.7 ng/mL), as compared to fish at 24 and 27 C (99.2 ± 15.5 ng/mL and 80.2 ± 12.8 ng/mL, respectively). Our findings indicate that the optimum temperature for rearing surubim in tanks is 27 C. At 30 C, these fish may suffer from stress, as suggested by the elevated peak of cortisol and morbidity and mortality rates. The relatively rapid cortisol stress response of surubim suggests that they may be more tolerant to handling stressors than other fish species.  相似文献   

19.
Diel cyclic hypoxia occurs with varying frequency and duration in freshwater habitats, yet little is known about its effects on reproduction of freshwater fishes. The present study shows that long-term exposure of goldfish (Carassius auratus) to cyclic hypoxia (0.8 ± 0.2 mg/l dissolved oxygen) for 9 h or more, per day, altered plasma lipid and sex steroid profiles, which in turn directly or indirectly suppressed ovarian growth and viable spermatozoa production. Hypoxia decreased total cholesterol and high density lipoprotein (HDL p < 0.05) and elevated triglycerides (TG; p < 0.05) in both sexes. Plasma steroid concentrations particularly of 17α-hydroxyprogesterone (17-HP), estradiol (E2), testosterone (T) in females, and T and 11-ketotestosterone (11-KT) in males were attenuated under diel hypoxic conditions. Intriguingly, both diel and continuous hypoxia elevated plasma E2 and vitellogenin levels in males. However, neither lipid nor steroid profiles recorded any variation in a dose-dependent manner in response to diel hypoxia. The reduced GSI, decreased number of tertiary oocytes, and motile spermatozoa in hypoxic fish clearly indicate suppression of gametogenesis. Thereby, prolonged diel cyclic hypoxia may affect valuable fishery resources and fish population structure by impairing reproductive performances and inducing estrogenic effects in males.  相似文献   

20.
The experiment consisted of three experimental groups: (1) “vaccine and stress”, (2) “stress and vaccine” and (3) control. All groups have previously been vaccinated 6 months prior to the start of the experiment. At the start of the experiment, the “vaccine and stress” group was vaccinated with Pentium Forte Plus for the second time (25.02.2008) and then given a daily stressor (confinement stressor 267 kg m-3 in 15 min) for a period of 4 weeks. The “stress and vaccine” group was given a similar daily stressor for 4 weeks and then vaccinated for the second time. The control group was neither stressed nor vaccinated a second time. The results indicates that fish in the “stress and vaccine” group may have entered an allostatic overload type 2 due to oversensitivity to ACTH, a reduced efficient negative feedback system with elevated baseline levels of plasma cortisol and reduced immune response with pronounced effects on the well-being of the animal. The “vaccine and stress” group may likewise have entered an allostatic overload type 1 response, with oversensitivity to ACTH and transient reduced efficient negative feedback system. This study shows that if plasma cortisol becomes elevated prior to vaccination, it could perhaps instigate an allostatic overload type 2 with dire consequences on animal welfare. To reduce the risk of compromising the animal welfare during commercial vaccination of salmon, one propose to grade the fish minimum a week prior to vaccination or grade simultaneously with vaccination. This could reduce the overall allostatic load during handling and vaccination and secure a healthy fish with intact immune response and improved animal welfare.  相似文献   

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