共查询到16条相似文献，搜索用时 164 毫秒
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为了分离鸡消化道中的乳酸菌,用BL和改良MRS培养基进行菌种分离,通过生化试验、乳酸纸层析等方法进行鉴定,结果自鸡嗉囊和盲肠中分离到产酸能力强的菌株8株,经鉴定为唾液乳杆菌、发酵乳杆菌和肠球菌为主。其中嗉囊中以唾液乳杆菌为主,盲肠中以发酵乳杆菌为主,其次是唾液乳杆菌和肠球菌。 相似文献
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试验旨在研究饲料中添加乳酸杆菌对黄河鲤和建鲤正反交F1生长性能的影响。在基础饲料中分别添加乳酸杆菌含量为0(A01、A02对照组)、2×108cfu/kg(A11、A12)、4×108cfu/kg(A21、A22)、6×108cfu/kg(A31、A32)、8×108cfu/kg(A41、A42),采用室内循环水养殖系统,在水温为27.5～28.0℃的条件下,选取平均尾重(8.25±0.15)g黄河鲤和建鲤正反交F1各600尾,随机分为10组,每组设3个重复,每个重复40尾,进行56 d的生长试验。结果表明:各乳酸杆菌组的末重、增重率和特定生长率均显著高于对照组(P<0.05),添加水平在6×108cfu/kg时的终末体重、增重率、特定生长率最大,且反交F1生长较快,饵料系数最低,添加水平再增加,终末体重、增重率、特定生长率降低,饵料系数增加;以终末体重、增重率、特定生长率、饵料系数为指标,采用二次多项式分析表明,黄河鲤、建鲤正反交F1饲料中乳酸杆菌的适宜添加水平为(5.8～6.0)×108cfu/kg。 相似文献
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发酵乳酸杆菌F6对鸡小肠上皮细胞β-防御素-9基因表达的影响 总被引：1，自引：0，他引：1
采用实时荧光定量PCR（fluorescence quantitative PCR,FQ-PCR）检测益生性发酵乳酸杆菌F6刺激鸡小肠上皮细胞后抗菌肽β-防御素-9（AvBD9）基因表达变化,为从益生菌与上皮细胞抗菌肽表达关系的新角度解析益生菌发挥益生作用的新途径和机制提供一定的基础及依据。利用不同剂量（2×105,2×106,2×107 CFU）的发酵乳酸杆菌F6分别刺激原代培养的鸡小肠上皮细胞4h,提取刺激后的细胞总RNA,反转录为cDNA,FQ-PCR检测抗菌肽AvBD9基因表达变化。结果表明,未受刺激的正常对照组也检测到AvBD9mRNA的表达,发酵乳酸杆菌F6能上调AvBD9基因表达。刺激组中AvBD9mRNA的表达在不同剂量组之间存在差异。2×105 CFU/mL组AvBD9mRNA的表达量极显著高于未受细菌刺激的对照组和2×106 CFU/mL组（P〈0.01）,显著高于2×107 CFU/mL组（P〈0.05）。2×106 CFU/mL组和2×107 CFU/mL组AvBD9mRNA的表达量显著高于未受细菌刺激的对照组（P〈0.05）,但2×106 CFU/mL组和2×107 CFU/mL组之间无显著差异（P〉0.05）。发酵乳杆菌F6与鸡小肠上皮细胞相互作用过程中可提高抗菌肽AvBD9mRNA的表达,且存在剂量依赖性。 相似文献
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本研究利用不同配方的培养基选择性分离、纯化不同生长营养需求的菌种,然后对所分离菌株作形态观察、生理生化鉴定,再用牛津杯法进行乳酸杆菌全菌液与上清液对大肠杆菌拮抗作用的对比试验。结果分别从鸡粪、牛粪、猪粪中分离、纯化出发酵乳杆菌、发状乳杆菌、木糖乳杆菌;乳酸杆菌培养上清液的拮抗作用大于全菌液;鸡源发酵乳杆菌对猪源大肠杆菌有较好的生物拮抗作用;猪源木糖乳杆菌及牛源发状乳杆菌对猪源大肠杆菌无生物拮抗作用。 相似文献
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Three gut lactobacilli from piglets (Lactobacillus plantarum L 5, Lactobacillus paracasei L 81, Lactobacillus fermentum L 670) and Lactobacillus casei subsp. pseudoplantarum L.c.) from a calf were examined by microtitre plate binding assay for their lectin-like binding activity after their cultivation on Rogosa agar and in MRS broth. Three ECM (extracellular matrix) molecules (fetuin, porcine fibronectin and porcine mucin) were selected for this assay. Additionally, the effect of heparin on the binding of these three ECM molecules by Lactobacillus strains in microtitre plates was tested. Moreover, haemagglutination tests with pig, cattle, sheep, and hen erythrocytes were performed. However, none of the four Lactobacillus strains examined did react with any of the erythrocytes tested. The differences between individual strains were observed in their binding to immobilised ECM molecules. The best adherent was the Lactobacillus plantarum L5, however, the other three strains showed also good ECM binding. With regard to an influence of cultivation medium on lectin-like binding activity, binding of all ECM molecules was expressed in Lactobacillus paracasei L 81 to significantly higher degree (P < 0.001) after cultivation on Rogosa agar than in MRS broth. Similarly, strains Lactobacillus fermentum L 670 and Lactobacillus casei subsp. pseudoplantarum L.c. displayed significantly higher (P < 0.001) binding of fibronectin and mucin after growth on Rogosa agar in comparison with MRS broth cultivation. However, no significant (on fetuin and fibronectin binding) or opposite effect (on mucin binding) of cultivation medium was observed in Lactobacillus plantarum L 5 strain. The influence of cultivation medium on fetuin binding by Lactobacillus fermentum L 670 was also not significant while Lactobacillus casei subsp. pseudoplantarum L.c. bound fetuin significantly better (P < 0.01) after growth on Rogosa agar. Heparin pretreatment increased the binding of the ECM molecules by the Lactobacillus fermentum L 670 strain significantly (P < 0.001 or P < 0.05) with the exception of porcine fibronectin when the strain was cultivated in MRS broth. This result is important especially in the connection with the previous observations that heparin decreased ECM binding of enteropathogens as staphylococci or clinical enterococcal isolates. Following up on some earlier strain characteristics, these results indicate that the selected lactobacilli are probably suitable for probiotic purposes. 相似文献
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为了取得比较理想的禽用微生态制剂菌种,用BL和乳酸杆菌选择性培养基从SPF鸡嗉囊和盲肠中分离乳酸菌,通过生化试验、乳酸纸层析等方法进行鉴定。结果,从SPF鸡嗉囊和盲肠中分离到产酸能力较强的细菌3株,经鉴定为2株乳酸杆菌(LactobaccillusPlanterum)、1株粪链球菌(Enterococcusfaecium)。 相似文献
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优良抑菌活性乳酸菌对玉米青贮及有氧暴露期微生物数量和pH的影响 总被引：1，自引：0，他引：1
为了探讨乳酸菌对全株玉米青贮及有氧暴露后青贮饲料中乳酸菌、好氧细菌、酵母菌和霉菌数量及其pH的影响,进一步筛选出可提高青贮饲料品质和有氧稳定性的乳酸菌接种剂,将实验室前期从甘肃各地玉米秸秆青贮饲料中分离筛选获得的5株产酸快、多且具有抑菌活性的优良乳酸菌分别添加全株玉米进行青贮,分析青贮过程和有氧暴露后青贮饲料中乳酸菌、好氧细菌、酵母菌和霉菌数量的动态变化及pH。结果显示,在青贮过程和有氧暴露后,分别添加肠膜明串珠菌肠膜亚种B1-7、戊糖片球菌B2-3、植物乳杆菌B3-1、屎肠球菌B5-2和发酵乳杆菌E2-3的各处理组乳酸菌总数均显著高于对照组,而好氧细菌、酵母菌和霉菌数量均显著低于对照组,pH亦低于对照组。其中B1-7和B5-2处理组在青贮初期乳酸菌总数最多,从青贮第7天开始到有氧暴露的30 d内,始终是B3-1处理组乳酸菌总数最多,好氧细菌、酵母菌和霉菌数量最少、pH最低。以上结果表明这5株乳酸菌具有提高青贮饲料品质和有氧稳定性的潜力,其中植物乳杆菌B3-1的效果最好。 相似文献