共查询到20条相似文献，搜索用时 187 毫秒
1.
2.
3.
除去精浆对波尔山羊冷冻精液品质的影响 总被引：2，自引：0，他引：2
以波尔山羊精液为实验材料,采用二步稀释法在降温前以葡萄糖-卵黄液为洗涤液,应用离心洗涤法对波尔山羊精液进行1000r/min离心处理,制作细管冻精,并进行超微结构分析.结果表明:经离心处理的冻精精子活率极显著优于对照组(P<0.01),精子顶体完整率极显著高于对照组(P<0.01) ,精子存活时间不同处理间未见显著差异(P>0.05).精浆中包含有多种物质,充当精子载体的作用,并可稀释精子,为精子提供代谢物质.试验中除去精浆后精子活率、 顶体完整率均优于对照组,表明除去精浆对冷冻后的精子品质有提高作用. 相似文献
4.
5.
为改善吐鲁番黑羊精液的冷冻保存效果,提高精液冻后的精子活率,本实验在3种精液冷冻稀释液中添加不同浓度(0、1%、2%)的十二烷基硫酸钠(SDS),TrⅠs-葡萄糖-柠檬酸钠为Ⅰ液,蔗糖-葡糖糖-柠檬酸钠为Ⅱ液,OptⅠdyl稀释液做对照为Ⅲ液。熏蒸冷冻后解冻,用精子分析系统(CASA)和流式细胞仪检测冷冻效果。结果表明:Ⅰ液1%SDS组精子活率最高(65.4%),与Ⅰ液2%SDS组精子活率没有差异,高于其他7组(P<0.05);Ⅲ液1%SDS组畸形率(9.4%)低于Ⅱ液0%SDS组、Ⅱ液2%SDS组和Ⅲ液2%SDS组(P<0.05);Ⅰ液1%SDS组直线运动速率(51.4μm/s)高于Ⅰ液0%SDS组、Ⅱ液2%SDS组和Ⅲ液0%SDS组(P<0.05);Ⅰ液1%SDS组和Ⅲ液1%SDS组的质膜完整率高于Ⅰ液2%SDS组和Ⅱ液1%SDS组(P<0.05),且Ⅰ液1%SDS组最高;Ⅰ液1%SDS组的顶体完整活精子比率高于Ⅱ液1%SDS组(P<0.05),各组的活精子顶体完整率无显著差异;Ⅰ液1%SDS组高线粒体膜电位比率(43.0%)高于Ⅱ液1%SDS组(P<0.05)。因此,采用添加1%SDS的Tris-葡萄糖-柠檬酸钠稀释液配方显著提高了吐鲁番黑羊精液冷冻保存效果。 相似文献
6.
7.
《中国畜牧兽医文摘》2015,(8)
通过对萨福克肉羊新鲜精液在离心前进行不同时间的静置,选择不同的离心速度及离心时间进行冷冻前预处理,以解冻后精子活率,复苏率为判断指标,比较了各种不同预处理对萨福克精液冷冻效果的影响。结果表明:离心前在37℃环境下静置60min的冻后效果(活率达0.47和复苏率达到73.8%)优于30min和90min的冻后效果(p0.05);离心过程以1000r/min的速度离心10min较好,冻后活率0.45,复苏率达到74.5%,优于500r/min和1500r/min转速和其他时间(p0.05)。 相似文献
8.
本试验采用不同的稀释液对兔精液进行冷冻保存,并比较了在稀释液中添加不同防冻剂以及平衡时间对兔精液冷冻保存效果的影响.结果表明:稀释液Ⅱ稀释的精液,解冻后,精子活率明显高于稀释液Ⅰ稀释的(P＜0.01),其复苏率显著高于后者(P＜0.05).以稀释液Ⅱ为基础液,添加不同浓度(27.3%,15%,12%,9%)的DMSO对兔精液冷冻保存,结果解冻后精子活率以15%及12%两组较好,其中添加15%的优于12%的,但差异不显著(P＞0.05).选择不同种类的防冻剂(7%甘油与15%DMSO)比较时,发现7%甘油组解冻后精子活率明显低于15%DMSO组,二者差异极显著(P＜0.01),其复苏率显著低于后者(P＜0.05).以15%DMSO作为防冻剂,采用不同的平衡时间在5 ℃平衡,结果发现平衡30 min和60 min的解冻后精子活率较好,但差异不显著(P＞0.05),而90 min和120 min的冷冻效果较差. 相似文献
9.
通过对不同配方的稀释液、添加不同剂量的维生素B12、维生素C和复合维生素B对解冻后精子存活影响的试验,结果表明,配方Ⅰ、Ⅱ、Ⅲ三种稀释液制作的冻精解冻后精子活率均大于30.00%,Ⅰ液最高,其冷冻精液解冻后活率可达43.35%±0.85%,但Ⅰ液和Ⅱ液差异不显著(P>0.05),Ⅲ液最低,差异显著(P<0.05);顶体完整率和畸形率Ⅰ液最优,差异显著(P<0.05);Ⅱ液与Ⅲ液差异不显著(P>0.05);在Ⅰ稀释液中添加维生素B12对解冻后精子的活率、精子顶体完整率影响不大,但平均值都高于对照组,并且随着维生素B12量的增加,精子畸形率有继续降低趋势;添加复合维生素B 4mL/100mL,维生素C 4mL/100mL对犬精子均有保护作用。 相似文献
10.
选用3种稀释液配方分别对6头种公牛精液进行稀释、封装、冷冻,并检查种公牛冻精解冻后的精子活力、畸形率、顶体完整率、冻后废弃率、存活时间及镜检感光度.结果表明：3种不同稀释液配方对种公牛精液冻后活力影响差异均显著（P＜0.05）,Ⅰ稀释液生产冻精的畸形率极显著高于Ⅱ稀释液和Ⅲ稀释液（P＜0.01）,3种稀释液冻精精子顶体完整率之间差异均极显著（P＜0.01）,Ⅲ稀释液与Ⅰ稀释液和Ⅱ稀释液间对牛精液所生产冻精的冻后废弃率影响均差异显著（P＜0.05）,Ⅰ稀释液与Ⅱ稀释液和Ⅲ稀释液间对牛精液所生产冻精的存活时间影响差异也显著（P＜0.05）,Ⅲ稀释液生产的冻精精子感光度优于Ⅰ稀释液和Ⅱ稀释液,其余各项指标差异均不显著（P＞0.05）.因此,可用自配Ⅱ稀释液替代进口专用Ⅲ稀释液. 相似文献
11.
用液氮熏蒸法在氟板上制作冻精颗粒,以解冻后的精子活率、活力和质膜完整性为判定指标,比较4种冷冻稀释液及不同冷冻-解冻程序对五指山小型猪精液冷冻的效果。结果表明:①Ⅳ号冷冻稀释液冷冻解冻后精子的活率(0.610±0.036)、活力(0.427±0.025)和质膜完整性(0.503±0.015)均显著高于Ⅰ、Ⅱ和Ⅲ号冷冻稀释液(P<0.05)。②实验中精液在4℃冰箱中平衡降温2 h的精液精子活力、质膜完整性均好于在17℃平衡3 h再放入4℃冰箱中平衡2 h的解冻效果,而且精子活率差异显著(P<0.05)。③湿解法的效果优于干解法。 相似文献
12.
Cow milk is used as an extender for ram semen cryopreservation. Caseins, the major proteins of milk, appear to provide some protective effect to sperm during cryopreservation. Goat milk has unique casein structure. The aim of this study was to investigate effect of goat milk, as a main semen extender, on freezability of Tushin Ram semen. For this aim, ejaculates from four Tushin rams were collected with artificial vagina and pooled. Pooled semen was separately extended with four different extenders: TRIS based (TRIS), cow skim milk based (CSM) (10 g/100 ml), cow semi‐skim milk based (CSSM) and goat semi‐skim milk based (GSSM) extenders, containing egg yolk and glycerol. The semen was cryopreserved and stored in liquid nitrogen until examination date. After thawing (at 37°C for 1 min), sperm motility, viability, morphology, acrosome and membrane integrity (HOST) were evaluated. Although, there was not any significant differences between extenders in post‐thaw percentage of viable spermatozoa (p > 0.05), Tushin ram semen extended with GSSM or CSM extenders had significantly higher post‐thaw percentage of progressive motility (25.0% and 30.8% respectively), compared with CSSM and TRIS (7.5% and 14.1% respectively, p < 0.001). Moreover, lowest abnormality percentage of post‐thaw spermatozoa were detected in ram semen extended with GSSM (49.5%) and CSM (51.5%), compared with CSSM (65.7%) and TRIS (60.7%) (p < 0.05). Whilst the results were considered, it was concluded that goat milk based extenders may be effectively and trustfully used in cryopreservation of Tushin ram semen, instead of cow milk and Tris based extenders, as a main extender. 相似文献
13.
Increasing interest in a longer preservation of diluted boar sperm raises questions in the field concerning the choice of the extender. The aim of this study was to evaluate the longevity of boar sperm extended in currently used commercial semen extenders. Three long-term extenders and two short-term extenders were compared for different semen quality parameters that can be assessed under routine laboratory conditions. Sperm morphology, motility, pH and bacteriological contamination were investigated during a 7-day period. The number of dead spermatozoa did not differ significantly among the extenders (p > 0.05). Sperm motility was not only related with storage period but most of all with pH, especially in long-term extenders. Differences between the different extenders were prominent (p < 0.05); the sperm preserved in only one long-term extender showed good motility during the whole test period. In all cases, the pH of the extended semen increased by 0.3-0.5 in the first days of storage and was significantly correlated with a decrease in motility. Bacteriological quality had no significant influence on motility or pH of the semen. In conclusion, we can state that in both short-term extenders and in only one long-term extender, sperm longevity, as evaluated by the parameters used in this study, was sufficient during the preservation period. To preserve the quality of diluted boar semen during long-term storage, the choice of the long-term extender is important. In addition, the monitoring of the pH of extended boar semen in our study emphasizes the importance of the buffering capacity of semen extenders. 相似文献
14.
Cryopreservation of Dog Semen in a Tris Extender with 1% or 2% Soya Bean Lecithin as a Replacement of Egg Yolk 
下载免费PDF全文
下载免费PDF全文 Egg yolk is usually included in extenders used for preservation of dog semen. Lecithin is an interesting animal‐protein free alternative to egg yolk for semen preservation. The aim of our study was to evaluate soya bean lecithin for cryopreservation of dog semen. Five ejaculate replicates were divided in three equal parts, centrifuged and each pellet diluted with one of the three Tris‐based extenders containing 20% egg yolk, 1% soya bean lecithin or 2% soya bean lecithin. Extended semen was loaded in 0.5‐ml straws, cooled and diluted a second time and frozen in liquid nitrogen vapours. Sperm motility parameters (CASA), acrosome integrity (FITC‐PNA/PI) and sperm membrane integrity (C‐FDA) were evaluated 5 min post‐thaw and after 2 and 4 h of incubation. Total motility was significantly better in the egg yolk extender than in any of the lecithin‐based extender and was better in the 1% lecithin extender than in the 2% lecithin extender. Sperm membrane integrity was significantly better in the egg yolk extender than in any of the lecithin‐based extenders but did not differ significantly between the 1% and 2% lecithin extenders. Acrosome integrity was significantly better in the egg yolk extender than in the 2% lecithin extender but did not differ between the egg yolk extender and the 1% lecithin extender or between the two lecithin extenders. In conclusion, egg yolk was superior to lecithin in our study. The extender with 1% lecithin preserved sperm motility better than the extender with 2% lecithin. 相似文献
15.
M Emamverdi M Zhandi A Zare Shahneh M Sharafi A Akbari‐Sharif 《Reproduction in domestic animals》2013,48(6):899-904
The purpose of the present study was to investigate the effects of a chemically defined soybean lecithin‐based semen extender as a substitute for egg yolk‐based extenders in ram semen cryopreservation. In this study, 28 ejaculates were collected from four Zandi rams in the breeding season and then pooled together. The pooled semen was divided into six equal aliquots and diluted with six different extenders: (i) Tris‐based extender (TE) containing 0.5% (w/v) soybean lecithin (SL0.5), (ii) TE containing 1% (w/v) soybean lecithin (SL1), (iii) TE containing 1.5% (w/v) soybean lecithin (SL1.5), (iv) TE containing 2% (w/v) soybean lecithin (SL2), (v) TE containing 2.5% (w/v) soybean lecithin (SL2.5) and (vi) TE containing 20% (v/v) egg yolk (EYT). After thawing, sperm motility and motion parameters, plasma membrane and acrosome integrity, apoptosis status and mitochondrial activity were evaluated. The results shown that total and progressive motility (54.43 ± 1.33% and 25.43 ± 0.96%, respectively) were significantly higher in SL1.5 when compared to other semen extenders. Sperm motion parameters (VAP, VSL, VCL, ALH and STR) were significantly higher in SL1.5 compared to other extender, with the exception of SL1 extender. Plasma membrane integrity (48.86 ± 1.38%) was significantly higher in SL1.5 when compared to other semen extenders. Also, percentage of spermatozoa with intact acrosome in SL1.5 (85.35 ± 2.19%) extender was significantly higher than that in SL0.5, SL2.5 and EYT extenders. The results showed that the proportion of live post‐thawed sperm was significantly increased in SL1.5 extender compared to SL0.5, SL2 and EYT extenders. In addition, SL1, SL1.5 and SL2.5 extenders resulted in significantly lower percentage of early‐apoptotic sperm than that in EYT extender. There were no significant differences in different semen extenders for percentage of post‐thawed necrotic and late‐apoptotic spermatozoa. Also, the results indicated that there are slight differences for percentage of live spermatozoa with active mitochondria between extenders. In conclusion, SL1.5 extender was better than other extenders in most in vitro evaluated sperm parameters. 相似文献
16.
S S Pérez-Garnelo M Oter C Borque C Talavera M Delclaux E Martínez-Nevado A T Palasz J De la Fuente 《Journal of zoo and wildlife medicine》2006,37(2):116-125
Basic characteristics of European bison (Bison bonasus) semen were described and the efficacies of two extenders-Triladyl, containing egg yolk, and a synthetic extender, containing soybean lipids-were tested for semen cryopreservation. Seven ejaculates were collected by electroejaculation from a 10-yr-old, European bison bull. Each ejaculate was diluted at 37 degrees C to a final concentration of 200 x 10(6) sperm/ml with Triladyl or the synthetic extender. Extended semen samples were frozen according to a standard bull semen freezing protocol. After 2 wk of storage, one straw from each extender and ejaculate was thawed, and postthaw quality was evaluated by individual sperm motility and movement rate, numbers of sperm morphologic abnormalities and intact acrosomes, functional integrity of the sperm membranes determined by hypoosmotic swelling test (HOST), viability (live-dead, eosin-nigrosin stain), and a heterologous in vitro sperm penetration assay (SPA). A total of 600 in vitro-matured bovine oocytes were inseminated with 1 X 10(6) spermatozoa of Holstein semen frozen-thawed in Triladyl (control) or of European bison semen frozen in Triladyl or the synthetic extender. Nuclear status of the oocytes was determined after 18 h of sperm-oocyte coincubation. Extender had no effect on any evaluated parameters of semen after dilution and cooling (4 hr at 5 degrees C) or in postthaw individual motility, quality of movement, and sperm morphology. However, significantly (P < 0.05) higher numbers of spermatozoa with intact acrosomes, intact membranes (HOST), and viable sperm (P < 0.01) were in semen frozen in Triladyl than in the synthetic extender. Mean values for heterologous SPA for bull (control) and for bison semen frozen in the synthetic extender were very much alike-63.3+/-10.6% and 63.1 +/- 15.9%, respectively; bison semen frozen in Triladyl was lower, 43.0+/-24.2% but not significantly different. Cumulative results from a variety of viability assays of diluted/cooled and frozen-thawed semen, including the heterologous SPA, suggest that European bison semen can be successfully frozen in both extenders tested in this study. 相似文献
17.
B‐T Zhao D Han C‐L Xu M‐J Luo Z‐L Chang J‐H Tan 《Reproduction in domestic animals》2009,44(6):865-872
A specific problem in the preservation of goat semen has been the detrimental effect of seminal plasma on the viability of spermatozoa in extenders containing egg yolk or milk. The use of chemically defined extenders will have obvious advantages in liquid storage of buck semen. Our previous study showed that the self‐made mZAP extender performed better than commercial extenders, and maintained a sperm motility of 34% for 9 days and a fertilizing potential for successful pregnancies for 7 days. The aim of this study was to extend the viability and fertilizing potential of liquid‐stored goat spermatozoa by optimizing procedures for semen processing and storage in the mZAP extender. Semen samples collected from five goat bucks of the Lubei White and Boer breeds were diluted with the extender, cooled and stored at 5°C. Stored semen was evaluated for sperm viability parameters, every 48 h of storage. Data from three ejaculates of different bucks were analysed for each treatment. The percentage data were arcsine‐transformed before being analysed with anova and Duncan’s multiple comparison test. While cooling at the rate of 0.1–0.25°C/min did not affect sperm viability parameters, doing so at the rate of 0.6°C/min from 30 to 15°C reduced goat sperm motility and membrane integrity. Sperm motility and membrane integrity were significantly higher in semen coated with the extender containing 20% egg yolk than in non‐coated semen. Sperm motility, membrane integrity and acrosomal intactness were significantly higher when coated semen was 21‐fold diluted than when it was 11‐ or 51‐fold diluted and when extender was renewed at 48‐h intervals than when it was not renewed during storage. When goat semen coated with the egg yolk‐containing extender was 21‐fold diluted, cooled at the rate of 0.07–0.25°C/min, stored at 5°C and the extender renewed every 48 h, a sperm motility of 48% was maintained for 13 days, and an in vitro‐fertilizing potential similar to that of fresh semen was maintained for 11 days. 相似文献
18.
Comparison of two different centrifugation extenders for preservation of frozen equine semen 下载免费PDF全文
下载免费PDF全文 M. Leão Freitas C. Silva Bouéres F. J. Gonçalves de Oliveira M. A. de Oliveira Viu R. Arruda de Oliveira 《Equine Veterinary Education》2015,27(8):410-413
This study compares a commercial semen extender (control group) to ultra high temperature (UHT) skimmed milk (treatment group) used during centrifugation for subsequent cryopreservation of equine semen. Following post‐thawing of semen samples parameters measured included motility, sperm motion kinetics (using computerised assisted semen analysis) as well as acrosome and plasmatic membrane integrity (using fluorescent dyes). After collection and analysis, the sperm‐rich fraction was divided and diluted with either: control (1:1 dilution in a skimmed milk‐glucose extender) or treatment (1:1 dilution in UHT skimmed milk). The milk used in this experiment was of the same source, commercial brand, of only one lot. After dilution, samples were subjected to centrifugation at 600 g for 10 min and sperm pellets were resuspended in a freezing extender to a concentration of 200 × 106 cells/ml. Aliquots were packed into 0.5 ml straws placed in a stainless steel support and kept inside the refrigerator (5°C) for 20 min. Subsequently, these straws were placed at a height of 6 cm over liquid nitrogen for 20 min in an isotherm box. No significant differences were observed in total sperm motility (42.71 vs. 38.29%), progressive sperm motility (12.29 vs. 7.86%), plasma membrane integrity (53.43 vs. 60.14%) or acrosomal membrane integrity (93.29 vs. 93.71%) with a P>0.05 calculated between the control and the treatment groups, respectively. Considering that UHT skimmed milk has a lower cost than the commercial semen extender, this could be an option used during the centrifugation protocol to decrease the expense of the equine semen cryopreservation process and increase shelf life. 相似文献
19.
C-L Xu J-B Zhou B-T Zhao G-C Lan M-J Luo Z-L Chang H-S Sui J-H Tan 《Reproduction in domestic animals》2009,44(5):771-778
The suitability of certain commercial and self‐made chemically defined extenders for liquid storage of goat semen was tested and the effects of storage temperatures, dilution rates and sperm washing and pH of extenders on the goat sperm during liquid storage were observed. Semen was collected from nine goat bucks of the Lubei White and Boer breeds using an artificial vagina. Each ejaculate after initial evaluation was diluted with a specific extender, cooled and stored at a desired temperature. Stored semen was evaluated for sperm motility and other parameters every 24 or 48 h of storage. The ranking order of the existing milk‐ and yolk‐free extenders in sustaining goat sperm motility was Androhep > Zorlesco > Beltsville thawing solution > the Tris–glucose medium. The new extender (mZA) which was formulated based on Zorlesco and Androhep was more suitable for goat sperm than Androhep. The mZAP extender with Bovine Serum Albumin (BSA) replaced with polyvinyl alcohol (PVA) worked as efficiently as the mZA in maintaining sperm motility, membrane integrity, acrosome intactness and capacitation status. Goat sperm motility was best maintained at 5°C during liquid preservation, but decreased significantly as the temperature increased. When semen was sixfold diluted, sperm motility was maintained longer (p < 0.05) after centrifugation, but sperm motility did not differ between the centrifuged and non‐centrifuged groups when semen was 11‐fold diluted. When the extender pH was adjusted from 6.6 to 6.04, the efficiency increased significantly in both Androhep and mZAP. A forward sperm motility of 34% was maintained for 9 days when buck semen was 11‐fold diluted and stored at 5°C in mZAP, with pH adjusted to 6.04. It is concluded that for liquid storage of buck semen, the mZA extender was more suitable than other extenders; BSA can be replaced with PVA in mZA; centrifugation to remove seminal plasma can be omitted by adequate dilution; and the storage temperature and pH of extenders affected sperm motility significantly. 相似文献
20.
The extenders and freezing rates from three different freezing protocols were combined and compared to each other in order to study the post-thawing acrosome integrity and fertility of frozen dog sperm. A commercial bovine TRIS-base extender (TRILADYL) and two self-made canine semen extenders (Norwegian and Dutch) were combined with a conventional bovine and two canine freezing regimes, and acrosome integrity of frozen/thawed spermatozoa was assessed by fluorescein isothiocyanate conjugated peanut agglutinin staining (FITC-PNA). Differences between freezing/thawing protocols were reflected in the proportion of cells with acrosomal damage and not based on motility results. It was concluded that during dog semen cryopreservation extenders had less influence on the post-thawing sperm quality than did the freezing rates. The optimal extender/freezing rate combination (TRILADYL/Norwegian) was used in the clinical practice to evaluate the fertility of frozen sperm administered by intrauterine insemination using a surgical approach. The pregnancy rate was 57% (4/7), but the average litter size was low (2.8). This may have been due to the insufficient sperm numbers contained in an insemination dose and/or to the incorrect timing of artificial insemination (AI). The final conclusion is that the commercial bovine extender is useful for freezing dog semen, and the TRILADYL/Norwegian freezing protocol is recommended as the most advantageous combination for the freezing of canine semen in the clinical practice. 相似文献