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1.
《中国兽医杂志》2017,(11)
采集河南地区规模化种鸡场疑似沙门菌致死鸡胚和肝脏,共分离出41株沙门菌菌株;应用血清学、VITEK 2和基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)方法对分离的41株沙门菌进行鉴定比较;3种方法均能鉴定出沙门菌,MALDI-TOF-MS方法能快速鉴定41株沙门菌,而且匹配度分值均大于2.300,表明鉴定结果的可信度很高。血清学鉴定的41株沙门菌血清型与MALDI-TOF-MS鉴定的沙门菌结果不一致,表明血清学分型结果和MALDI-TOF-MS分型结果没有相关性。MALDI-TOF-MS对鸡沙门菌的鉴定,操作快速简便、准确率高,为兽医微生物快速鉴定提供一种方法。 相似文献
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肯塔基沙门菌（Salmonella Kentucky）是继鼠伤寒沙门菌和肠炎沙门菌后又一与人类肠道疾病相关的沙门菌血清型，因其获得和传播耐药性的能力较强而逐渐引起关注。该血清型沙门菌按照多位点序列分型分得的众多亚型中，ST198血清亚型具有易获得耐药突变的基因组岛的特性，21世纪以来不断进化，除获得常见抗菌药物耐药基因外，还相继获得对氟喹诺酮类和碳青霉烯类药物的耐药性并扩散全球，造成临床上难以治疗的病例而引起世界卫生组织的关注。作者整理了肯塔基沙门菌的进化历程，着重阐述肯塔基沙门菌关于氟喹诺酮类及碳青霉烯类药物的耐药情况及耐药机制，以期为中国沙门菌的实验室防控监测系统提供理论依据。 相似文献
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本研究旨在建立和优化禽沙门菌分离鉴定的方法。选择山东省某地方品种鸡疑似发生沙门菌病的未出壳死胚样品24份,通过4组不同的培养基组合比较沙门菌的分离情况,选择沙门菌通用引物两对和鸡白痢特异性引物一对进行分离株的PCR检测,比较其特异性,然后进行血清分型和MLST分型,确定沙门菌类型并研究其相关性。结果表明,该批样品中TTB比SC增菌效果好,XLD和XLT4选择培养基对沙门菌分离效果相同,最终通过PCR方法鉴定出沙门菌阳性率为58.3%;发现两对通用引物特异性一致,表明可任选一对引物进行PCR鉴定;用鸡白痢沙门菌特异性引物完成的PCR鉴定结果显示,14株沙门菌中有7株为鸡白痢沙门菌;血清型鉴定结果表明,14株沙门菌中有7株鸡白痢沙门菌、7株肠炎沙门菌,共两种血清型;MLST分子分型结果表明,14株沙门菌分为3个ST型,分别是7株ST11、3株ST92和4株ST2151,其中ST11为肠炎沙门菌,ST92和ST2151为鸡白痢沙门菌;本研究表明,疑似沙门菌感染样品经BPW和TTB增菌、XLD或XLT4选择培养基分离培养,最后通过PCR方法可准确完成沙门菌的分离鉴定;同时,应用该方法可完成对鸡白痢沙门菌的鉴定,并与血清分型和MLST分子分型结果一致。 相似文献
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《动物医学进展》2016,(2)
为了研究鸡白痢沙门菌SpiC蛋白的功能,以鸡白痢沙门菌S06004基因组DNA为模板PCR扩增spiC基因,进行克隆测序和生物信息学分析,并调查该基因在255株不同年代鸡白痢沙门菌分离株中的分布与进化情况。结果显示,鸡白痢沙门菌spiC基因全长384bp,编码127个氨基酸,在不同年代分离株中稳定存在。推导SpiC蛋白无跨膜区、信号肽和卷曲螺旋结构,有1处酪蛋白激酶Ⅱ磷酸化位点,2处N-豆蔻酰化位点,预测线性抗原表位主要分布在15-20、29-35、52-53、70-72、83-85、89-94aa。利用SWISS MODEL在线程序建模,得到SpiC三维结构模型。同源性及进化分析显示spiC基因仅存在于沙门菌中且比较保守,鸡白痢沙门菌SpiC蛋白与沙门菌肠道亚种亲缘关系最近。 相似文献
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通过对饲料中的沙门菌进行分离和鉴定、血清型分型和脉冲场凝胶电泳分型,分析其存在和分布的情况,为分析饲料中沙门菌产生的原因,建立追踪溯源方法,并为其防治提供参考。按GB/T 13091进行分离、鉴定;参考GB 4789.4进行血清型分型;PFGE参照PulseNet的标准方法。分离、鉴定得到15株沙门菌,检出率为14.3%;血清型的出现频次依次为德尔卑、阿贡纳、里森等;PFGE结果显示,15株沙门菌相似度在38.8~100%之间,相同血清型的菌株聚类后,一般都能分到同一群,表明血清型与PFGE指纹图谱有一定相关性;随着同一批次检出沙门菌份数的增加,PFGE聚类结果越趋于集中,与血清型分析结果一致。沙门菌监测应设定科学的抽样份数;血清型和PFGE聚类结果的分布随着检出份数的增加差异越来越小,可能为风险爆发,PFGE可作为沙门菌风险溯源的方法;在一个血清型内,沙门菌既有较高的基因同源性,又具有遗传多态性。 相似文献
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同时感染雏鸡群的两种血清型沙门菌的鉴定及药敏试验 总被引：2，自引：2，他引：0
沙门菌是肠杆菌科中的一个大属,目前已有2500种以上血清型,它们广泛存在于人和各种动物的肠道内。在自然界中,家禽是其最主要的贮存宿主。禽沙门菌病是由沙门菌属中的某一种或多种沙门氏菌引起的禽类急性或慢性疾病的总称,由鸡白痢和鸡伤寒沙门菌引起的疾病分别称为鸡白痢和鸡伤寒,而由有鞭毛能运动的沙门菌引起的疾病则称为禽副伤寒。本试验系应用细菌学和PCR检测技术,对安徽省濉溪县一起可疑雏鸡沙门菌病进行细菌分离鉴定,从而确定病型类别,并对分离菌株实施血清学分型和药物感受性分析。 相似文献
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禽沙门菌病是家禽养殖领域中的常见病与高发病,给家禽产业中造成严重的经济损失,同时也严重威胁着禽产品消费者的健康安全。文章从沙门菌分类学、禽沙门菌流行血清型、快速检测技术以及分子分型技术等方面阐述了当前禽沙门菌的研究进展,以期为禽沙门菌病的防治提供参考。 相似文献
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为了分析都江堰地区冷鲜肉食品中沙门菌的生物学特性,从都江堰地区冷鲜肉食品加工厂、超市等采集冷鲜肉食品样品53份。采用细菌分离、形态学观察、培养特性、生化试验和PCR等方法对采集的53份样品进行沙门菌检测,结果显示,从53份冷鲜肉食品样品中分离到30株沙门菌。致病性试验结果显示,24株分离菌株为致病性沙门菌。采用玻板凝集试验检测沙门菌血清分型情况,结果显示,分离的30株沙门菌包括8种血清型,其中以猪霍乱沙门菌、肠炎沙门菌、鼠伤寒沙门菌为主要流行血清型,分别占分离菌株的23.3%、20%、20%。耐药性结果显示,30株沙门菌分离菌株对阿莫西林、氨苄西林、新霉素等6种药物耐药性比较严重,耐药率在50.0%~100%之间,且呈现多重耐药性。表明都江堰地区冷鲜肉食品中沙门菌污染严重,血型分型呈现多样性分布,耐药性严重,呈现多重耐药性,应引起重视。 相似文献
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《中国预防兽医学报》2018,(10)
为分析GenBank中登录的国内外空肠弯曲菌及其亚种中所含CRISPR-Cas系统的结构特征及同源关系,本研究利用生物信息学方法预测GenBank中已登录的空肠弯曲菌所含的CRISPR-Cas系统的基因序列,对其进行比对、保守性分析、RNA二级结构预测、重复序列的GC含量分析及其遗传进化分析。结果显示,空肠弯曲菌及其亚种中所含CRISPR-Cas系统的重复序列高度保守、GC含量较低(28.57%以下),差异性主要呈现于两端游离部分;CRISPR中的间隔序列差异性较大致使CRISPR具有多态性;Cas蛋白序列同源性较高(96.8%以上),遗传动态变化较低,与基因水平转移产生的趋同进化有潜在相关性。空肠弯曲菌CRISPR-Cas系统中的间隔序列具有多态性与该菌的遗传进化差异有关,重复序列及cas基因保守性较强,与其共同进化有潜在相关性。通过对不同空肠弯曲菌CRISPR-Cas系统生物信息学分析,为进一步研究该菌CRISPR-Cas系统结构特征、基因进化关系奠定了基础。 相似文献
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Ovine scrapie was first recorded in Cyprus in 1985. Subsequently four dairy goats kept in two mixed flocks with affected sheep developed characteristic clinical signs similar to those seen in sheep. Fifteen goats from the two flocks were examined histologically and neurological lesions consistent with a diagnosis of scrapie were found in the four animals and in three others which had subsequently developed early neurological signs. These lesions were similar to those of naturally-affected sheep although neuronal degeneration and vacuolation were more severe in some cases. 相似文献
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Owen J Slocombe D McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1986,27(9):324-328
In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%).
Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada.
相似文献14.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1989,30(6):504-508
In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%). 相似文献
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Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1985,26(10):323-327
In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal.
Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection.
相似文献16.
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In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated.
Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively.
相似文献18.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1988,29(8):641-646
In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%). 相似文献
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Owen J Slocombe D McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1987,28(8):491-495
In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%).
Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada.
相似文献20.