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1.
将8头荷期坦供体奶牛随机分为4组,每组2头,分别按优势卵泡+注射促卵泡素(FSH)、优势卵泡+不注射FSH、去优势卵泡+注射FSH、去优势卵泡+不注射FSH处理供体牛,处理后第4天应用B型超声波导引活体采卵,试验重复10次。结果显示:注射FSH前去优势卵泡对其可采卵泡数、回收卵母细胞数和可用卵母细胞数均有显著提高(P0.05),但不注射FSH时去优势卵泡对提高采卵效率无显著效果;活体采卵对供体牛卵巢虽造成一定损伤,但停止采卵2~3个月后均能恢复正常发情、配种后能正常妊娠,对供体牛繁殖性能无显著影响。 相似文献
2.
[目的]研究和牛母牛超数排卵及胚胎移植效果。[方法]利用纯种和牛母牛作为供体,荷斯坦母牛及西杂母牛作为受体,通过供体牛的超数排卵、人工授精、采胚、胚胎冷冻及受体牛的同期发情和鲜胚移植,快速获得纯种和牛及和牛胚胎。[结果]对4头青年纯种和牛母牛进行超排,重复冲卵13头次,共采卵数122枚,其中75枚可用胚胎,可用胚率为61.5%,平均5.76枚/头次。对75头荷斯坦牛及西杂母牛同期发情,结果获得89.2%的同期发情率。67头受体牛移植了鲜胚,2个月后妊娠检查有35头怀孕,移植妊娠率为52.23%。[结论]可以利用超数排卵及胚胎移植增加和牛母牛利用效率。 相似文献
3.
为建立BMY牛的活体采卵(OPU)-体外胚胎生产(IVP)-胚胎移植(ET)快速扩繁技术体系,从人工草地全放牧牛群中选择健康的经产空怀BMY牛作为供体(n=14)集中OPU一次;卵母细胞经TCM-199体外成熟培养(IVM)、Fert-TALP液体外受精(IVF),受精卵用合成输卵管液(SOF)体外培养(IVC)生产胚胎;BMY牛受体(n=81)用CUE-MATETM孕酮阴道栓+EB+FSH+PG方法同期发情处理,对黄体合格的移植OPU-IVP来源鲜胚。研究结果表明:①OPU头均获卵母细胞23.50枚,获10枚以上卵母细胞的供体占85.7%;IVP的平均囊胚数和可用胚数分别为5.21和4.00枚;OPU前8～9d卵巢上有黄体(CL)的供体,头均获卵母细胞数及IVP的囊胚数、可移植胚数、囊胚率和可移植胚率分别比对照组高7.28枚(P<0.05)、7.29枚(P<0.05)、6.00枚(P<0.05)、27.3%(P<0.01)和23.2%(P<0.01);②受体黄体合格率69.1%(56/81),胚胎移植妊娠率37.5%(21/56),产犊率35.7%(20/56);③受体在胚胎移植时注射GnRH,其妊娠率比对照组高16.2%(P>0.05)。结果显示,BMY母牛具有良好的OPU潜力,但个体间差异较大;可通过严格供体选择及改善IVP技术等措施,实现OPU-IVP效率最大化。CUE-MATETM+EB+FSH+PG同期发情处理方法适于在牛规模化胚胎移植(ET)和人工授精(AI)中应用。综合应用OPU、IVP、同期发情和ET技术是加快优良BMY牛扩繁的重要途径之一。 相似文献
4.
采用肌注PG 0.6 mg和宫注PG 0.3 mg两种方案对冲胚后供体牛进行处理,其目的是筛选出对母牛生殖恢复既有效又经济的方案.结果表明:方案一:采用肌注PG方式,供体母牛在肌注PG后第2～11天出现发情,主要集中在处理后第2天和第4～8天,所占比例91.66%.方案二:采用宫注PG方式,处理后第3～8天和第17天都有牛发情,主要集中在处理后第3～8天,所占比例94.12%.实验结论:PG肌注与PG宫注对母牛生殖恢复均有效果,从溶解黄体和经济效益两方面综合评价,采用含PG 0.3 mg溶液宫注,既可达到较好的溶解黄体效果,又节约了成本,在生产实践中具有较好的推广价值. 相似文献
5.
我所从2003年开始，在所属奶牛场利用生产性能优良的荷斯坦经产母牛和育成牛作为供体牛生产胚胎，生产的胚胎用于“广西牛胚胎移植技术推广”项目。在4年多共使用122头供体牛冲胚，冲胚后我们采取一系列的技术措施，使供体牛从冲胚后到发情配种受孕年龄、间隔天数、配种次数和年受胎率等受孕力取得较好的效果，现分析如下。 相似文献
6.
《上海畜牧兽医通讯》2016,(2)
采用杂交奶水牛及纯种奶水牛作为供体牛,将日本生产的FSH作为外源激素进行超数排卵,以确定胚胎移植供体牛超排的效果;采用CIDR+PMSG+PG+LHRH-A3法,确定受体牛胚胎移植和同期发情的效果。试验结果表明:5头奶水牛供体牛超排,共采卵7头次,采卵数21枚,其中10枚可用胚胎,平均1.4枚/头次,可用胚率为47.6%。受体牛的同期发情效果和可移植率分别为77.0%和62.0%。 相似文献
7.
胚胎移植是继家畜人工授精、同期发情之后21世纪最重要的家畜繁殖新技术之一。但目前通过超排和非手术采卵技术获得的可用胚相对较少,一般每头牛获得的可用胚平均在5枚左右,这严重影响了受体母牛的利用率。通过对移植西门塔尔牛分割鲜双半胚和全胚妊娠率的对比试验,为胚胎移植技术的推广提供参考依据。1材料与方法1.1时间与地点试验从2004年7月18日开始,在贵州省种公牛站进行。1.2药品试剂CIDR孕酮阴道栓,新西兰生产;FOLLTROPINV,加拿大生产;氯前列烯醇(PG),上海计划生育科学研究所生产,每支含PG0.2mg;HCG,宁波第二激素厂生产;兽用… 相似文献
8.
2005年3月至7月,在广西地区选择25头娟姗母牛作为供体,用不同厂家、不同剂量的促排卵激素进行超排和重复超排,观察了超排及重复超排效果.结果25头供体共采卵46头次,排卵数502枚,回收到278枚胚胎,采卵率为55.4%,获得207枚可用胚胎,平均4.5枚/头次,可用胚率为74.5%;与此同时在2005年4月至2006年1月,开展了娟姗牛冷冻胚胎的移植工作,对185头本地黄母牛和杂交母牛进行了同期发情处理,结果共有166头发情,其中113头可移植,同期发情率为89.7%,可移植率为61.1%.113头可移植牛移植2个月后,经妊娠检查共有47头怀孕,移植妊娠率为41.6%. 相似文献
9.
奶牛超数排卵及胚胎移植的试验研究 总被引：1，自引：0，他引：1
本试验采用FSH＋PG的方法对22头供体荷斯坦牛分两批进行了超数排卵处理,140头受体南阳黄牛和供体牛采用2次PG法分批进行了同期发情处理。结果如下：（1）共有20头供体牛、104头受体牛在第二次注射PG后96h内发情,同期发情率分别为90.90%和74.29%;（2）20头供体牛回收胚胎176枚,可用胚胎140枚/头,平均回收胚胎8.8枚/头,平均可用胚胎7.0枚/头,可用胚胎比率79.55%;（3）104头南阳黄牛受体均移植单胚,51头妊娠,妊娠率为49.04%。 相似文献
10.
为挖掘利用具有优秀遗传性能的淘汰母牛的卵泡资源，本实验开展淘汰牛连续活体采卵的效果及影响因素研究。选取6头5～6岁无卵巢疾病的淘汰西门塔尔牛，分别于7、10、11月和12月每间隔4 d进行1次活体采卵，每月最高进行5次活体采卵，记录每头次的卵泡抽吸次数、获卵数、优质卵数，并对影响活体采卵效果的因素进行分析。结果表明：平均头次获卵数和优质卵数分别为4.61枚和2.22枚，卵子回收率和优质卵率分别为47.21%和49.13%；试验牛的平均头次获卵数无显著差异，但第1次活体采卵的卵子回收率高于第2～5次（P<0.05），第2次活体采卵的优质卵率显著高于第5次；采卵月份对平均头次获卵数、回收率和优质卵率无显著影响；采卵次数与采卵月份间无交互作用（P>0.05）。综上，淘汰牛连续活体采卵的效果相对稳定，连续采卵次数对卵子回收率、优质卵率具有一定影响。 相似文献
11.
目的 研究旨在通过试验观察中草药治疗奶牛子宫内膜炎疗效,以其为临床应用中草药治疗奶牛子宫内膜炎提供参考。方法 将60 头患子宫内膜炎奶牛,随机分成5 组,每组12 头,复方中草药高、中、低剂量组（分别子宫灌注150 mL/头、100 mL/头和50 mL/头）、土霉素组（子宫灌注50 mL/头）和对照组。结果 复方中草药中剂量组和高剂量组的总有效率为75.00%和83.33%,均高于土霉素组的总有效率66.67%;土霉素组在治疗结束后的平均发情天数缩短了14 d,所有复方中草药组的发情天数也缩短了11~19 d;3 个情期内土霉素组受胎率为50.00%,复方中草药组受胎率介于41.67%~66.67%之间。结论 复方中草药对奶牛子宫内膜炎的治疗效果优于土霉素,其中以高剂量组效果最佳。 相似文献
12.
Mohammad Rahim Ahmadi Meysam Makki Abdolah Mirzaei Hamid Reza Gheisari 《Reproduction in domestic animals》2019,54(5):762-771
The objective of this study was to compare the reproductive performance of cows affected by clinical endometritis (CE) following treatment with an intrauterine infusion of 50% dextrose solutions (DEX) and liquid paraffin (LP) as alternative therapies with routine treatments including PGF2α injection and intrauterine infusion of oxytetracycline (OTC). Moreover, the reproductive indices of cows treated with endometritis were compared with those of healthy ones. At postpartum reproductive tract examination (28–35 DIM) in one Iranian dairy farm, cows with CE without any selection were assigned to four groups: (a) OTC, a common treatment in Iran, was administered (5 g) to 396 cows, (b) PGF2α (PG) was injected to 496 cows, (c) dextrose solution (DEX): intrauterine infusion of 50% dextrose solution (200 ml) was done in 427 cows, and (d) liquid paraffin (LP) was administered (100 ml) to 423 cows via intrauterine route. We further assigned 2,233 clinically healthy cows to the control group. The incidence of endometritis was 41.6% in this study. Based on the results of reproductive indices including median days to first AI, days open (DO), first service conception rate, conception rate in 2nd and 3rd services, conception rate in all three services, pregnancy rate < 100 days and pregnancy rate < 200 days, except for median days to first AI in other reproductive indexes, reproductive performance was significantly lower in LP group compared with the healthy cows and other treatment groups (p < 0.05). Except for the first service conception rate and proportion of cows pregnant before 100 days in milk (DIM), there existed no significant difference between the DEX group and the control as far as reproductive performance is concerned (p ≥ 0.05). The first service conception rate was recognizably lower in DEX group compared with OTC and PG (p < 0.05). In conclusion, the use of a non‐antibiotic special solution of dextrose 50% is a good alternative to antibiotic agents concerning the treatment of CE in dairy cows. 相似文献
13.
14.
清宫散对患子宫内膜炎奶牛血液流变学指标的影响 总被引：1，自引：0，他引：1
采用椎板式血液粘度仪检测了中药方剂-清宫散对患子宫内膜炎奶牛治疗前后血液流变学指标的影响。结果表明:患子宫内膜炎奶牛的全血粘度、全血低切/高切还原粘度、血浆粘度、红细胞聚集指数、红细胞刚性系数显著或极显著高于健康牛,经清宫散治疗后上述指标显著或极显著下降;空白对照组奶牛首次采血时的血液流变学参数与试验组奶牛治疗前的参数无显著差异(P>0.05);各组奶牛红细胞压积无显著差异(P>0.05)。结论:清宫散能改善患牛微循环障碍,降低血液粘度,进而达到活血化瘀之功效。 相似文献
15.
为了进一步探明中草药添加剂对奶牛繁殖性能的确切影响,将60头经产奶牛随机分成对照组和各用1个试验药方的3个试验组,进行了2个繁殖周期的试验。结果表明：与对照组相比,3个试验组的第一情期受胎率分别提高8%、4.4%、4.4%;产后70 d内第一次发情的母牛比率分别提高13.3%、6.7%、6.7%;分娩至产后第一次配种间隔天数分别缩短了3.8、4.7、0.9 d;产犊间隔缩短了15、9、7 d;临床乳腺炎发病率降低了13.3%、20%、20%。由此可见,这3个试验药方都选药正确、配伍合理、效果确实,可在生产中推荐使用。 相似文献
16.
Drillich M Bergmann J Falkenberg U Kurth A Heuwieser W 《DTW. Deutsche tier?rztliche Wochenschrift》2002,109(9):386-390
Two methods for a scheduled post partum examination were compared on a commercial dairy farm. In Group 1, all cows (n = 601) were examined by rectal palpation between day 20 and 26 post partum for signs of endometritis (vaginal discharge, enlarged uterus). In Group 2, all cows (n = 652) were examined by external inspection for vaginal discharge as a sign for endometritis. In both groups all cows with endometritis were treated twice with prostaglandin F2 alpha (25 mg of dinoprost) in a 14-day interval. The proportion of cows with signs of endometritis was 33.3% and 17.2% in Group 1 and Group 2, respectively (p < 0.05). In Group 1, the conception rate (39.0% vs 49.3%) and the proportion of cows pregnant (60.5% vs 72.6%) were lower for cows with endometritis than for cows without endometritis. In Group 2, no significant differences were found in reproductive performance for cows with and without signs of endometritis. No significant differences in reproductive performance were found for cows with endometritis between the two groups. Also, for cows without endometritis no significant differences in reproductive performance were found between the groups. It is concluded that the post partum examination by rectal palpation was more sensitive in finding cows with endometritis. However, with regard to the reproductive performance the more sensitive method was not more effective than the method based on systematic external inspection. 相似文献
17.
The aim of the following investigation was to study the effect of beta-carotin serum concentrations on reproductive performance in dairy cows. Blood samples were collected from 201 cows 3 to 4 weeks antepartum, 1 to 2 weeks postpartum and at the time of insemination. Concentrations of beta-carotin in blood serum were determined via HPLC. The incidence of retained placental fetal membranes, endometritis, ovarian cysts as well as the beginning of the oestrous cycle and various measures of fertility such as first service conception rate, pregnancy index, interval from calving to first insemination, interval from calving to conception, interval from first insemination to conception and expected calving interval were recorded and analysed. The beta-carotin serum concentrations were not related to the incidence of retained placental fetal membranes, endometritis, ovarian cysts or the onset of cyclicity post partum. Cows with lower beta-carotin concentrations partially seemed to have a reduced reproductive performance than cows with higher beta-carotin concentrations. Overall, our results suggest only a minor relationship between the beta-carotin serum concentration and fertility in dairy cows. 相似文献
18.
The objectives of this study were to determine the risk factors for postpartum endometritis by evaluating several reproductive factors in individual cows, and to determine the effect of endometritis on the subsequent reproductive performance in dairy herds in Korea. The data, including health status, parity and body condition score (BCS) of cows, and calving date, were collected from 320 cows in eight dairy herds calving from January 2001 to October 2002. We used logistic regression to evaluate the effects of these factors on endometritis. A stepwise procedure, used to obtain the appropriate model with alpha=0.05, revealed that retained placenta, metabolic disorders and parity are the important risk factors for endometritis. The mean intervals from calving to first service and conception were prolonged (P<0.01) by 23 and 36 days, respectively, in the endometritis group compared to the non-endometritis group. The first service conception rate was lower (P<0.05) in the endometritis group (47.6%) than in the non-endometritis group (62.6%). The number of services per conception was higher (P<0.05) in the endometritis group (1.9) than in the non-endometritis group (1.6). We conclude that retained placenta, metabolic disorders and cow parity are strongly correlated with the development of postpartum endometritis, which decreases reproductive performance in dairy herds in Korea. 相似文献
19.
本试验旨在研究基础日粮中添加复方中草药对围产后期奶牛产奶量及乳成分的影响,为更好地发挥奶牛生产性能提供理论依据。选用围产期荷斯坦牛32头,按照年龄、胎次、预产期相近的原则,随机分成四组,对照组饲喂常规基础日粮,处理A组、处理B组、处理C组添加由黄芪、党参、香附、白术、当归、川穹、白芍、熟地等组成的复方中草药,产前21d至产后21d连续饲喂42d,添加量分别为50g／d、100g／d、150g／d。试验结果表明,处理A组、处理B组、处理C组与对照组相比,产奶量分别提高了2．74kg、4．87kg、4．73kg,差异显著（P〈0．05）,表明复方中草药能显著提高奶牛产奶量。基础日粮中添加复方中草药对乳成分的影响无明显变化规律,处理A组、处理B组、处理C组的干物质含量、乳脂率、乳蛋白率、乳糖率与对照组相比差异不显著（P〉0．05）。 相似文献
20.
A field trial was conducted to elucidate the effect of the time of initiation of a repeated PGF2alpha-application in a 14 day interval for treatment of endometritis in dairy cows. On a commercial dairy farm in Brandenburg, Germany, a total of 494 dairy cows were examined by rectal palpation and adspection for signs of endometritis (vaginal discharge, enlarged uterus) between day 20 to 26 post partum (dpp). We performed two further examinations by rectal palpation and external adspection to monitor the puerperal phase (34.-40. dpp, 55.-61. dpp). All cows with symptoms of an endometritis were treated with PGF2alpha (0.15 mg R-Cloprostenol, Preloban, Intervet Deutschland GmbH Unterschleissheim) twice in a 14-day interval. In the group "Early" (n = 146) the first injection of Cloprostenol was administered at time of the 1st examination. In the group "Late" (n = 129) an identical treatment was administered in cows with endometritis, however it was started 14 days later (34.-40. dpp). The incidence of endometritis was 57.7% in the group "Early" and 53.5% in the group "Late" at the first time of examination. The 1st service conception rates for treated cows were 34% in the group "Early" vs. 37% in the group "Late". In the group "Early" differences were found in days open between treated cows with endometritis and untreated controls without symptoms of endometritis (99.1 d vs. 110.8 d, p > 0.05). In the group "Late", days open for treated (106.8 d) and untreated cows (108.0 d) were similar. The severity of endometritis influenced the percentage of cows pregnant at 200 dpp. Regarding cows with a severe endometritis (E2 and E3) the percentage of pregnant cows 200 dpp was higher in the group treated early (E2: 78.4%; E3: 80.0%) than in the group with the late initiation of the treatment (E2: 68.6%; E3: 54.5%, p < 0.05). Cows with a moderate endometritis (E1) had a similar percentage of pregnant cows (200 dpp) as the untreated cows without endometritis. It is concluded that application of PGF2alpha in the 4th and 6th week post partum in a 14 day interval in cases of severe endometritis is more effective than the application of the same treatment two weeks later. 相似文献