共查询到20条相似文献，搜索用时 359 毫秒
1.
采用冷冻加束缚大鼠,观察情绪的表露对应激性大鼠脑组织脂质过氧化作用及5-羟色胺(5-HT)的影响.结果显示,大鼠正常冷冻束缚后,脑组织丙二醛(MDA)和5-HT含量显著升高,谷胱甘肽过氧物酶(GSH-Px)和超氧化物歧化酶(SOD)活性明显下降.而情绪的表露处理的大鼠可使应激时脑组织中MDA和5-HT含量显著降低,GSH-Px和SOD活性接近于正常室温下的对照组水平.上述结果提示,紧张情绪和行为的变化可增强脑组织的脂质过氧化作用,而情绪的表露能有效抵御这种脂质过氧化作用,增强脑组织对自由基的清除能力,且情绪与行为的变化和脑组织中5-HT的变化直接相关. 相似文献
2.
情绪表露对大鼠脑组织脂质过氧化作用及5—HT的影响 总被引：2，自引：0，他引：2
李倬 《动物科学与动物医学》2001,18(6):20-21
采用冷冻加速缚大鼠，观察情绪的表露对应激性大鼠脑组织脂质过氧化作用及5－羟色胺（5－HT）的影响。结果显示，大鼠正常冷冻束缚后，脑组织丙二醛（MDA）和5－HT含量显著升高，谷胱甘肽过氧物酶（GSH－Px）和超氧化物歧化酶（SOD）活性明显下降。而情绪的表露处理的大鼠可使应激时脑组织中MDA和5－HT含量显著降低，GSH－Px和SOD活性接近于正常室温下的对照组水平。上述结果提示，紧张情绪和行为的变化可增强脑组织的脂质过氧化作用，而情绪的表露能有效抵御这种脂质过氧化作用，增强脑组织对自由工的清除能力，且情绪与行为的变化和脑组织中5－HT的变化直接相关。 相似文献
3.
4.
采用束缚浸水法建立大鼠应激性溃疡模型，探讨了胃泌素、胰高血糖素、血栓纱Ｂ２（ＴＸＢ２）的变化与与应激性溃疡相关性及应激宁的抑制效应。结果表明，应激组血浆、胃组织的３处激素水平的均极显著的高于对照组，应激＋应激宁组与应激组相比较，３处激素的水平极显著或显著降低，或降低不显著，提示这３种激素可能从不同角度参与应激性溃疡的形成，同时，应激宁对应激性溃疡的形成具有抑制作用。 相似文献
5.
6.
动物机体的各个不同器官、组织，是一个统一的整体，某一点或局部的变化，将引起连锁反应，所以应以“综合症”概述之较为准确。猪应激性综合症在养殖过程中无时不在，深入了解其发生及作用机制，有利于改善当前的养殖条件，减少应激性病变，提高生猪的综合生产性能，实现养殖增收。本文将对猪应激性综合症的原因进行分析并提出相关的防控措施，减少或预防应激，从而相对增加养猪效益。 相似文献
7.
应激宁对HSP70在大鼠应激性溃疡中表达的影响 总被引：2，自引：0，他引：2
为了探讨热休克蛋白70(HSP70)在应激性溃疡中表达的变化及应激宁对其变化的影响,选用Wistar大鼠水浸-束缚应激(WRS)4 h的方法,建立应激性溃疡模型.用免疫组织化学方法检测胃黏膜组织HSP70的表达.结果表明,HSP70的表达主要分布在胃腺区,对照组大鼠胃黏膜组织中有散在的表达,应激组中HSP70阳性细胞数目较应激宁组增多,两者具有明显的差异(P＜0.05),而且,应激组和应激宁组中HSP70阳性细胞数目均比对照组明显增多(P＜0.01).表明应激宁对WRS后胃黏膜组织HSP70的表达具有调节作用,这也表明应激宁具有抗应激的作用. 相似文献
8.
杨昊天 陈永平 王志强 黄宇翔 马志刚 邹跃 魏念冬 张红 李鑫 董佳强 吕明哲 李洪彬 刘力威 杨昊轩 张国华 刘雪松 钟鹏 石荷叶 寇玉红 陈志峰 《畜牧兽医学报》2023,54(2):779-786
本研究拟探索右美托咪定（dexmedetomidine,DEX）对大鼠急性应激致肾损伤的保护作用,并从氧化应激的角度探索DEX对大鼠肾的保护通路。本研究使用了急性束缚应激模型,其中,大鼠被迫游泳15 min,并束缚3 h。本试验采用生化检测、组织病理学切片观察以评估肾功能,然后测定了氧化应激以及氧化应激的相关通路蛋白。旷场试验证实成功建立了急性应激模型。急性应激引起的肾损伤增加了NOX4,降低了Nrf2/HO-1/NQO1表达水平。DEX可降低NOX4表达,同时升高Nrf2/HO-1/NQO1的表达水平。DEX治疗组与急性应激组相比的肾生化结果明显恢复正常,病理切片观察损伤显著降低。试验结果表明,DEX治疗急性应激可影响NOX4/Nrf2/HO-1/NQO1信号通路,并抑制氧化应激。因此,DEX对急性应激引起的肾损伤具有保护作用,并在应激综合征中具有潜在的临床应用。 相似文献
9.
大脑眶额叶(OFC)是与情绪密切相关的脑区,试验主要通过强迫游泳制造应激抑郁大鼠模型,免疫组织化学方法观察模型鼠大脑OFC及其他脑区Fos蛋白表达变化,以揭示OFC区与应激性抑郁症之间的关系。结果与对照组比较,强迫游泳应激后大鼠眶额叶、海马、丘脑室旁核、杏仁内侧核等多个脑区均有Fos蛋白高表达,差异极显著(P0.01)。强迫游泳应激后OFC区Fos蛋白高表达,结果表明,OFC区参与了应激性抑郁症的发生。 相似文献
10.
甜菜碱对猪生产性能的影响及应用 总被引：2，自引：0，他引：2
甜菜碱广泛存在于动植物体内,植物中以在甜菜中的含量最高.甜菜碱在动物体内作为一种高效活性甲基供体,在甲基代谢中起重要的营养调控作用,可取代部分蛋氨酸和胆碱,具有促进脂肪代谢、改善饲料的适口性、缓和热应激、调节机体渗透压的作用,并可维护维生素预混料的稳定性. 相似文献
11.
12.
Doherty TJ Andrews FM Blackford JT Rohrbach BW Sandin A Saxton AM 《Equine veterinary journal》2003,35(5):472-475
REASONS FOR PERFORMING STUDY: Endotoxaemia causes a disruption of gastrointestinal motility in the horse but there is no information on its effects on gastric secretion. Lipopolysaccharide (LPS) administration is known to affect gastric secretion in other species. HYPOTHESIS: That LPS, a toxic component of Gram-negative bacteria, would reduce gastric acid secretion and that pretreatment with phenylbutazone (PBZ) would block the effects of LPS. METHODS: The effects of LPS and PBZ on gastric contents were investigated in fasted, mature horses, with permanent gastric cannulae. Horses were pretreated with either saline or PBZ 15 mins before a 60 min infusion of either LPS or saline. Gastric contents were collected at 15 min intervals for 3 h, beginning 15 mins after the start of the LPS or saline infusion. RESULTS: Lipopolysaccharide significantly decreased gastric acid output, [K+] and potassium output and increased [Na+] and sodium output. Phenylbutazone did not affect basal gastric acid secretion but decreased LPS-induced changes in the secreted volume, [Na+] and sodium output. CONCLUSIONS: This study provides evidence that LPS affects gastric acid secretion in the horse and that these LPS-induced changes are mediated, in part, by prostaglandins. POTENTIAL RELEVANCE: Lipopolysaccharide administration can induce changes in the composition of gastric contents in the horse but further work is needed to determine the source of these changes. 相似文献
13.
MARY A. McLOUGHLIN DVM MS RICHARD WALSHAW BVMS Dipiomate ACVS MICHAEL W. THOMAS DVM MS Dipiomate ACVR JOE G. HAUPTMAN DVM MS Dipiomate ACVS 《Veterinary surgery : VS》1992,21(1):33-39
Gastric conduit urinary diversion was performed in 10 dogs after complete cystectomy. Four dogs were euthanatized on day 30 because of hypochloremic metabolic alkalosis and renal failure. Hematologic and biochemical changes in six dogs evaluated for 120 days were compatible with hypochloremic metabolic alkalosis. The continuous loss of hydrochloric acid from the gastric conduit resulted in significant increases in arterial blood pH, PaCO2, anion gap, TCO2, and the concentration of HCO3-. There were significant decreases in PaO2 and the serum concentrations of chloride and potassium. Deterioration of renal function resulted in all dogs. It was concluded that hypochloremic metabolic alkalosis makes gastric conduit urinary diversion unsatisfactory for clinical use in dogs. 相似文献
14.
15.
The effects of oral administration of calcium chloride solutions to dairy cows were studied. When a 0.3 per cent solution was given ad libitum, and as the sole source of water for a period of 75 days, we observed no significant changes in feed consumption, body weight or milk production. The average daily water intake was increased by approximately 20 per cent, and signs of slight gastro-intestinal irritation were seen. No major changes in blood hemoglobin levels, hematocrits, total and differential white cell counts or thrombocyte numbers could be attributed to the treatment. We observed no significant effect on the serum calcium, chloride, magnesium, potassium, or sodium content. The level of inorganic phosphate in the serum rose to higher, but still normal values. During the entire experiment the urine pH was abnormally acid for dairy cows. Electrocardiograms were taken after 45 days of calcium chloride administration, and they appeared normal. When 0.1 and 0.2 per cent solutions were given as the sole source of water for a period of 81 days, the cows remained in good condition, and we observed no changes in appetite, body weight or milk production. In general, dairy cows will refuse to drink calcium chloride solutions when the concentration exceeds 0.5 per cent, even when they have been deprived of water for 18-24 hours. On the other hand, since the administration of lower concentrations for periods of 75 and 81 days did not cause any clinical sign of disease, it seems that on farms where more than one source of water are usually available, poisoning of dairy cattle by calcium chloride in solution is quite unlikely. 相似文献
16.
Pentagastrin stimulated gastric secretion in the dog (orogastric aspiration technique) 总被引：1，自引：0，他引：1
An orogastric aspiration technique is presented for the estimation of pentagastrin stimulated gastric secretion in the anaesthetised dog. Reference values for volume, pH and hydrogen ion output, 'acidity' and hydrochloric acid output, chloride, sodium, potassium and pepsin outputs were measured and calculated in 23 clinically healthy dogs. It was concluded that 30 and 45 minute hydrogen ion, hydrochloric acid and chloride peak outputs were the most suitable parameters for the acid secreting capacity of the parietal cells. Reference values for 30 and 45 minute peak outputs were respectively 1.63 to 3.61 and 1.50 to 3.35 mmol per hour per kg0.75 (hydrogen ion), 1.86 to 3.64 and 1.65 to 3.42 mmol per hour per kg0.75 (hydrochloric acid) and 2.35 to 4.13 and 1.98 to 3.91 mmol per hour per kg0.75 (chloride). Reproducibility of pepsin outputs was so poor that they cannot be used as parameters for gastric secretory capacity. 相似文献
17.
Effects of chloride conductance inhibitors on fluid secretion into ligated ileal and jejunal loops in pigs 总被引：1，自引：0，他引：1
Compounds that prevent chloride transport in membrane vesicles have been tested for in vivo activity against the effects of intestinal secretory agents. Chloride channel blockers including diphenylamine-2-carboxylate, 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonate, 5-nitro-2-(2-phenylethylamino)benzoic acid, and alpha-phenylcinnamic acid were tested for effects on jejunal or ileal secretion in weanling pigs. Secretion was studied in ligated intestinal loops in a control state, during exposure to secretory concentrations of theophylline, and after prior treatment with cholera toxin. Increases in net fluid flux induced by either theophylline or cholera toxin were not prevented by adding chloride channel blockers into the intestinal lumen. Channel blocker concentrations that reduced chloride transport by greater than 50% in pig jejunal brush border vesicles did not cause significant changes in unidirectional blood to lumen chloride flux measured in situ. Several routes of administration of the specific chloride channel blocker alpha-phenylcinnamate failed to reduce fluid secretion induced by theophylline. Chloride channel blocker effectiveness appears to be significantly different between in vitro and in vivo experimental models. In contrast to the chloride channel blockers, loperamide significantly reduced net fluid and chloride flux in ileal loops secreting fluid in response to theophylline. Antagonism of the production or actions of second messenger by loperamide was more effective than the chloride channel blockers in reducing conductive chloride transport associated with intestinal secretion. 相似文献
18.
采用Bliss法对小鼠经口灌胃氯化镉溶液（对照组0.0mg/kg;试验组Ⅰ～Ⅵ分别为98.3,122.9,153.6,192.0,240.0,300.0mg/kg）,并对染毒后小鼠的死亡情况、体质量、脏器指数、血液生理指标和病理剖检变化进行分析,以探讨氯化镉对小鼠的急性毒性效应。结果显示,氯化镉LD50为187.03mg/kg;小鼠的体质量随染毒剂量的增加而降低（P〈0.01）;与对照组相比,Ⅰ组小鼠心指数差异不显著（P〉0.05）,其余各组差异显著（P〈0.05）,脾指数和睾丸指数差异极显著（P〈0.01）,其余脏器指数均差异不显著（P〉0.05）;小鼠血液生理指标均在正常生理范围内波动（P〉0.05）;死亡小鼠除大脑组织无明显病变外,以Ⅵ组小鼠各组织的病变较严重,存活小鼠各组织的病变以Ⅰ组较轻微,Ⅴ组较严重。结果表明,氯化镉属于中等毒性物质,对小鼠多个组织均具有不同程度的急性毒性效应。 相似文献
19.
Gastric ulcers are common in horses and equine gastric ulcer syndrome describes ulcers in the distal oesophagus, nonglandular and glandular stomach as well as the proximal duodenum. Ulcers in the distal oesophagus and nonglandular region of the stomach are probably caused by hydrochloric acid, whereas ulcers in the glandular stomach and proximal duodenum are likely to be caused by a breakdown in the mucosal defence mechanisms. Regardless of the location of ulcers, initial treatment with pharmacological agents is necessary to suppress hydrochloric acid; however, nutrition and management changes are required to maintain ulcer healing and help prevent recurrence. In addition, many dietary supplements have been marketed to help maintain stomach health and some scientific data on their effects on gastric ulcer scores and gastric juice pH are presented. 相似文献
20.
To study the effect of luteolin on blood indexes,liver and kidney in mice with acute mercury poisoning,28 mice were randomly divided into four groups:Control group (intraperitoneal injection 0.9% saline),luteolin group (lavage 100 mg/kg luteolin),mercuric chloride group (intraperitoneal injection 4 mg/kg mercury chloride) and mercury chloride+luteolin group (intraperitoneal injection 4 mg/kg mercuric chloride,lavage 100 mg/kg luteolin).The activities of ALT in serum,AST,CREA and BUN contents,blood WBC,RBC,HGB content and GSH and MDA contents of liver tissue were detected.Morphological changes of liver and kidney tissues were observed.The results showed that compared with the control group,the activities of ALT and AST of mercuric chloride group were extremely significantly increased (P < 0.01),serum CREA,BUN,blood WBC and liver tissue MDA contents were significantly increased (P < 0.05) while blood RBC,HGB and liver tissue GSH contents were significantly decreased (P < 0.05).Liver,kidney pathological changes were obviously.Compared with mercuric chloride group,the activities of ALT,AST in serum,CREA,BUN,blood WBC and liver tissue MDA contents of mercuric chloride+luteolin group were significantly decreased (P < 0.05),while blood RBC,HGB and liver tissue GSH contents were significantly elevated (P < 0.05).Liver,kidney pathological changes were attenuated obviously.The poisoning were characterized by inflammation and the occurrence of anemia when acute mercury poisoning occurred,liver and kidney showed different degrees of injury in mice.Luteolin could reduce the toxic effects of acute mercury poisoning on blood,liver and kidney. 相似文献