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The purpose of this study was to investigate the effects of compound bacteria preparation (including homogeneous Lactobacillus/heterogeneous Lactobacillus, Bacillus subtilis and Aspergillus niger as well as their compatibility) on nutrient composition, fermentation quality and rumen fermentation characteristics of Phragmites australis silage. Taking growing Phragmites australis from Dongting lake as silage material, five groups were set up, which were blank group (CK group), lactic acid bacteria group (LA group, supplemented with Lactobacillus plantarum, Pediococcus pentosaceus and Lactobacillus buchneri), lactic acid bacteria+Bacillus subtilis group (LA+BS group), lactic acid bacteria+Aspergillus niger group (LA+AN group) and lactic acid bacteria+Bacillus subtilis+Aspergillus niger group (LA+BS+AN group), and each group had 3 replicates. The supplemental level of lactic acid bacteria was 1.5×108 CFU/kg silage raw materials, and the supplemental levels of Aspergillus niger and Bacillus subtilis were 1.0×108 CFU/kg silage raw materials (all were based on fresh weight of silage raw materials). After 60 days of silage, the nutrient composition and fermentation quality of Phragmites australis silage were determined, and the rumen fermentation characteristics were evaluated by in vitro fermentation and in vivo degradation. The results showed as follows: 1) the contents of dry matter and ash in LA, LA+BS and LA+AN groups were significantly lower than those in CK group (P<0.05), and the crude protein content in LA+BS, LA+AN and LA+BS+AN groups was significantly higher than that in CK and LA groups (P<0.05). 2) The silage sensory evaluation scores of all groups were 60 to 70 points, and the ratings were good. The comprehensive scores of fermentation quality in LA+BS and LA+AN groups were higher, among which, the ammonia nitrogen to total nitrogen ratio, acetic acid content and acetic acid to total acid ratio in LA+AN group were significantly decreased compared with CK group (P<0.05), while the lactic acid content and lactic acid to total acid ratio were significantly increased compared with CK group (P<0.05). 3) The dry matter disappearance rate and total gas production of in vitro fermentation in LA+AN group were significantly higher than those in the other groups (P<0.05), and in vitro fermentation parameters such as volatile fatty acid contents in fermentation liquid were improved. 4) The rumen fermentation dry matter degradation rate in LA+AN and LA+BS+AN groups at 8, 24 and 48 h was significantly higher than that in the other three groups (P<0.05). In conclusion, the combined application of lactic acid bacteria and Aspergillus niger with supplemental levels in this experiment has the best effect on the quality of Phragmites australis silage and rumen fermentation characteristics. © The Author(s) 2023. 相似文献
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超临界二氧化碳萃取杜仲果实中桃叶珊瑚苷工艺研究 总被引:1,自引:2,他引:1
采用超临界CO2流体萃取技术选择适合的夹带剂,进行了从杜仲果实中提取桃叶珊瑚苷的工艺研究.运用L16(45)正交表,较系统地探讨了各因素对提取效果的影响,提取液以Epstahl试剂显色用分光光度法进行分析.研究表明:与传统的溶剂提取法相比,超临界CO2萃取法具有操作简单、活性成分保存好、后续分离易于进行等特点;在萃取中以75 %的乙醇为夹带剂,当萃取压力26 MPa、萃取温度55 ℃、分离温度30 ℃、萃取时间120 min、夹带剂用量与原料比6∶1(mL∶g)的条件下,效果最佳,桃叶珊蝴苷得率1.921 %. 相似文献
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以不同品种杏鲍菇为试材,研究在培养料中添加不同浓度的亚硒酸钠,培养富硒杏鲍菇。通过对杏鲍菇子实体中硒含量、各种生理特性指标的测定。分析不同品种的杏鲍菇对硒的富集能力,硒对杏鲍菇子实体的过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶的活性影响。结果:对硒的富集能力不同品种间存在差异。培养料中亚硒酸钠含量在50~100mg/kg时,易于子实体中硒的富集,各种酶的活性增强,当亚硒酸钠含量在150~200mg/kg时,MDA(丙二醛)含量高于对照。结论:适宜的硒含量易于杏鲍菇硒的富集及生理特性的改善。 相似文献
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