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The purpose of this study was to investigate the effects of compound bacteria preparation (including homogeneous Lactobacillus/heterogeneous Lactobacillus, Bacillus subtilis and Aspergillus niger as well as their compatibility) on nutrient composition, fermentation quality and rumen fermentation characteristics of Phragmites australis silage. Taking growing Phragmites australis from Dongting lake as silage material, five groups were set up, which were blank group (CK group), lactic acid bacteria group (LA group, supplemented with Lactobacillus plantarum, Pediococcus pentosaceus and Lactobacillus buchneri), lactic acid bacteria+Bacillus subtilis group (LA+BS group), lactic acid bacteria+Aspergillus niger group (LA+AN group) and lactic acid bacteria+Bacillus subtilis+Aspergillus niger group (LA+BS+AN group), and each group had 3 replicates. The supplemental level of lactic acid bacteria was 1.5×108 CFU/kg silage raw materials, and the supplemental levels of Aspergillus niger and Bacillus subtilis were 1.0×108 CFU/kg silage raw materials (all were based on fresh weight of silage raw materials). After 60 days of silage, the nutrient composition and fermentation quality of Phragmites australis silage were determined, and the rumen fermentation characteristics were evaluated by in vitro fermentation and in vivo degradation. The results showed as follows: 1) the contents of dry matter and ash in LA, LA+BS and LA+AN groups were significantly lower than those in CK group (P<0.05), and the crude protein content in LA+BS, LA+AN and LA+BS+AN groups was significantly higher than that in CK and LA groups (P<0.05). 2) The silage sensory evaluation scores of all groups were 60 to 70 points, and the ratings were good. The comprehensive scores of fermentation quality in LA+BS and LA+AN groups were higher, among which, the ammonia nitrogen to total nitrogen ratio, acetic acid content and acetic acid to total acid ratio in LA+AN group were significantly decreased compared with CK group (P<0.05), while the lactic acid content and lactic acid to total acid ratio were significantly increased compared with CK group (P<0.05). 3) The dry matter disappearance rate and total gas production of in vitro fermentation in LA+AN group were significantly higher than those in the other groups (P<0.05), and in vitro fermentation parameters such as volatile fatty acid contents in fermentation liquid were improved. 4) The rumen fermentation dry matter degradation rate in LA+AN and LA+BS+AN groups at 8, 24 and 48 h was significantly higher than that in the other three groups (P<0.05). In conclusion, the combined application of lactic acid bacteria and Aspergillus niger with supplemental levels in this experiment has the best effect on the quality of Phragmites australis silage and rumen fermentation characteristics. © The Author(s) 2023. 相似文献
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为研究过瘤胃半胱胺和复合酶制剂对后备湖羊母羊生长性能和血液生化指标的影响，试验选用2月龄、体重（19.00±0.86）kg、健康的湖羊75只，随机分为5组，每组3个重复，每个重复5只羊。对照组（T1组）饲喂基础日粮，试验组分别在基础饲粮中添加0.16%过瘤胃半胱胺（T2组）、0.16%复合酶制剂（T3组）、0.16%过瘤胃半胱胺和0.16%复合酶制剂（T4组）。预试期7 d，正式期60 d。结果表明：（1）与对照组相比，T2组和T4组的终末体重分别提高了15.35%和14.81%(P <0.01),T2组、T3组和T4组的平均日增重分别提高了30.97%、12.26%、27.74%(P <0.01)，平均日采食量分别降低了4.33%、3.95%、3.10%(P <0.05)，耗料增重比分别降低了26.98%、14.51%、24.22%(P <0.05)。（2）与对照组相比，T2组、T3组和T4组的生长激素水平分别提高了12.01%、12.24%、11.85%(P <0.01),T3组和T4组的胰岛素生长样因子1水平分别提高了17.48%和21.37%(P ... 相似文献
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家畜致病菌的快速检测对疾病防控十分重要。基于形态学、免疫学、细菌毒力、分子生物学等传统致病菌检测方法因操作繁琐、耗时长等技术壁垒问题，限制了家畜致病菌快速检测。近年来，微流控技术与多种检测技术相结合，实现了样品到结果的一键快速检测，解决了对仪器和人员的要求问题，且具备高通量、微型化、集成化、高灵敏性等优点，适合多种环境下的家畜致病菌快速检测。论文概述了家畜致病菌检测的传统检测方法以及微流控芯片技术在该领域的应用，并对其应用前景和发展趋势进行了展望。 相似文献
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血吸虫病是一种危害性极大的人畜共患寄生虫病，易感动物包括牛、羊、猪等哺乳动物。系统综述了家畜血吸虫病的生活史、传播链和临床症状，分析了血吸虫病的流行病学特征、诊断技术和综合防治措施，并提出了家畜血吸虫病综合防治性建议，为家畜血吸虫病的控制和治疗提供指导。 相似文献
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