Hydrocephalus secondary to obstruction of the lateral apertures in two dogs          下载免费PDF全文

M Kent  EN Glass  AC Haley  LS Shaikh  M Sequel  U Blas‐Machado  TM Bishop  SP Holmes  SR Platt 《Australian veterinary journal》2016,94(11):415-422

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First Production of Larvae Using Cryopreserved Sperm: Effects of Preservation Temperature and Cryopreservation on European Eel Sperm Fertilization Capacity          下载免费PDF全文

JF Asturiano  SR Sørensen  L Pérez  P Lauesen  J Tomkiewicz 《Reproduction in domestic animals》2016,51(4):485-491

Sperm cryopreservation is a useful tool in captive fish reproduction management, that is to synchronize gamete production, especially in the case of species as the European eel, where the time of female spawning readiness is unpredictable. Several protocols to cryopreserve sperm of this species have been described, but until recently fertilization trials were not feasible. This study evaluated the effect of cold storage of diluted sperm prior to fertilizations and tested whether a previously defined protocol for European eel sperm cryopreservation can be successfully applied in fertilization trials to produce viable offspring. In our experiment, the sperm motility was evaluated after the extraction and the best samples were selected and pooled. Until stripping of eggs and fertilization, diluted sperm samples were maintained at either 4 or 20°C, or cryopreserved, following existing protocols. Fertilization of two egg batches was attempted. Diluted sperm caused a similar percentage of fertilized eggs and a similar number of embryos and larvae, independently of storage temperature (4 or 20°C). The cryopreserved sperm resulted in a lower percentage of fertilized eggs, but embryos developed and a few larvae (‘cryolarvae’) were obtained 55 h after fertilization in one of the two egg batches. This result evidences that the tested cryopreservation protocol is applicable for eel reproduction management, although improvements will be required to enhance fertilization success.  相似文献   

Poultry Health and Management     

SR Raidal 《Australian veterinary journal》2000,78(12):830-830

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Stocking density and its influence on shape of Senegalese sole adults   总被引：1，自引：1，他引：0  

Pedro Pablo Ambrosio  Corrado Costa  Pablo Sánchez  Rosa Flos 《Aquaculture International》2008,16(4):333-343

Morphological changes in Senegalese sole adults (Solea senegalensis Kaup, 1858) reared at two stocking density conditions (low stocking density, 60% of bottom occupation and high stocking density, 180% of bottom occupation) were investigated using geometric morphometrics. Canonical variate analysis on weight matrix scores, including the uniform component (W′), at the end of the experiment revealed differences in body shape between experimental groups. The low density group presented a similar change pattern as that of the high density group but was less intense. Differences were discussed in terms of the effect of a high stocking density on the shape of soles which were very close to commercialization. Results did not indicate a significant size-related shape, likely due to fish age. Our results provide the first promising look at environment-related shape changes in reared sole.  相似文献   

Impact of the Mediterranean Urban Environment on Photosynthetic Efficiency of Quercus ilex Leaves     

Flavia De Nicola  Anna Alfani  Nicola D��Ambrosio 《Water, air, and soil pollution》2011,220(1-4):151-160

Environmental constrains and anthropogenic changes can contribute simultaneously to the limitation of plant performance in Mediterranean urban areas. Photosynthetic efficiency of Quercus ilex L. leaves from the urban area of Naples was evaluated by comparison with leaves from remote site (Vesuvius National Park). The photosynthetic performance of leaves with different age (current year [CY], 1- [1Y] and 2-year old [2Y]) was assessed by measurements of gas exchange, chlorophyll fluorescence and pigment contents. The photosynthetic activity at the urban site was higher than that at the remote site for the CY leaves, while the opposite was observed for the 1Y and 2Y leaves. The growth of Q. ilex trees was mainly sustained by the CY leaves at the urban site, whereas at the remote site the photosynthetic activity was not affected by leaf ageing. In the urban environment, Q. ilex leaves strongly decreased the photosynthetic performance with leaf ageing as highlighted by leaf gas exchanges, although the photochemistry did not show any significant change. The mature leaves of urban site drove the reductive power deriving from the photochemistry more in non-assimilative processes rather than in carbon assimilative ones, avoiding a strong decrease of PSII photochemical efficiency.  相似文献   

Circannual variation in plasma adrenocorticotropic hormone concentrations and dexamethasone suppression test results in Standardbred horses,Andalusian horses and mixed-breed ponies     

NJ Bamford  PA Harris  SR Bailey 《Australian veterinary journal》2020,98(12):616-621

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Localization and Bioreactivity of Cysteine-Rich Secretions in the Marine Gastropod Nucella lapillus     

Mariaelena D&#x;Ambrosio  Ctia Gonalves  Mariana Calmo  Maria Rodrigues  Pedro M. Costa 《Marine drugs》2021,19(5)

Marine biodiversity has been yielding promising novel bioproducts from venomous animals. Despite the auspices of conotoxins, which originated the paradigmatic painkiller Prialt, the biotechnological potential of gastropod venoms remains to be explored. Marine bioprospecting is expanding towards temperate species like the dogwhelk Nucella lapillus, which is suspected to secrete immobilizing agents through its salivary glands with a relaxing effect on the musculature of its preferential prey, Mytilus sp. This work focused on detecting, localizing, and testing the bioreactivity of cysteine-rich proteins and peptides, whose presence is a signature of animal venoms and poisons. The highest content of thiols was found in crude protein extracts from the digestive gland, which is associated with digestion, followed by the peribuccal mass, where the salivary glands are located. Conversely, the foot and siphon (which the gastropod uses for feeding) are not the main organs involved in toxin secretion. Ex vivo bioassays with Mytilus gill tissue disclosed the differential bioreactivity of crude protein extracts. Secretions from the digestive gland and peribuccal mass caused the most significant molecular damage, with evidence for the induction of apoptosis. These early findings indicate that salivary glands are a promising target for the extraction and characterization of bioactive cysteine-rich proteinaceous toxins from the species.  相似文献   

A Comparative In Vitro Evaluation of the Anti-Inflammatory Effects of a Tisochrysis lutea Extract and Fucoxanthin     

Elisabetta Bigagli  Mario D&#x;Ambrosio  Lorenzo Cinci  Alberto Niccolai  Natascia Biondi  Liliana Rodolfi  Luana Beatriz Dos Santos Nascimiento  Mario R. Tredici  Cristina Luceri 《Marine drugs》2021,19(6)

In this study, we compared the effects of a Tisochrysis lutea (T. lutea) F&M-M36 methanolic extract with those of fucoxanthin (FX) at equivalent concentration, on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. The T. lutea F&M-M36 methanolic extract contained 4.7 mg of FX and 6.22 mg of gallic acid equivalents of phenols per gram. HPLC analysis revealed the presence of simple phenolic acid derivatives. The T. lutea F&M-M36 extract exhibited a potent and concentration-dependent inhibitory activity against COX-2 dependent PGE2 production compared to FX alone. Compared to LPS, T. lutea F&M-M36 extract and FX reduced the expression of IL-6 and of Arg1 and enhanced that of IL-10 and of HO-1; T. lutea F&M-M36 extract also significantly abated the expression of NLRP3, enhanced mir-223 expression and reduced that of mir-146b, compared to LPS (p < 0.05). These findings indicate that T. lutea F&M-M36 methanolic extract has a peculiar anti-inflammatory activity against COX-2/PGE2 and NLRP3/mir-223 that might be attributable to the known anti-inflammatory effects of simple phenolic compounds found in the extract that may synergize with FX. Our data suggest that T. lutea F&M-M36 may serve as a source of anti-inflammatory compounds to be further evaluated in in vivo models of inflammation.  相似文献   

Trichomonas gallinae in budgerigars and columbid birds in Perth, Western Australia   总被引：1，自引：0，他引：1  

T. MCKEON  J. DUNSMORE  SR RAIDAL 《Australian veterinary journal》1997,75(9):652-655

Objective To estimate the prevalence of infection with Trichomonas gallinae and other parasites of the alimentary tract in psittacine and columbid birds in Perth and to determine in vitro the effectiveness of drugs commonly recommended for treating trichomoniasis.
Design and procedures Samples of crop contents were collected from aviary flocks of budgerigars (Melopsittacus undulatus) and other psittacine and columbid birds in both private and commercial collections in Perth. Similar samples from wild Senegal doves (Streptopelia senegalensis) also were collected. Crop contents were examined and cultured for Trichomonas gallinae and in vitro studies were conducted on the susceptibility of isolates to several drugs used commonly. Other parasites also were detected by faecal examination and/or necropsy.
Results T gallinae was recovered from birds in 1 of 13 private collections of budgerigars (2/289 birds in total). Direct wetmount examination of crop fluid identified 36.4% of samples at four commercial bird dealers which were later determined by culture to contain T gallinae . The prevalence of T gallinae infection ranged from 0 to 11.4% in budgerigars. The prevalence of T gallinae infection in wild Senegal doves was 46% and from one flock of racing pigeons was 59%. The in vitro minimum lethal concentrations of metronidazole, dimetridazole and ronidazole ranged from 40 to 96, 30 to 80 and 40 to 92 μg/mL respectively for six isolates of T gallinae . Other alimentary parasites detected during the survey included Spironucleus sp (syn Hexamita sp), coccidia, Ascaridia platycerci and Raillietina sp.
Conclusions Thirteen budgerigar flocks belonging to members of avicultural societies in Perth had a low prevalence of trichomoniasis and other parasitic infections. The dose rate currently recommended for ronidazole may not result in complete protozoacidal activity against T gallinae infection.  相似文献   

An Anaplasma centrale DNA probe that differentiates between Anaplasma ovis and Anaplasma marginale DNA     

E S Visser  R E Ambrosio  D T de Waal 《Veterinary microbiology》1991,28(3):313-325

An Anaplasma centrale genomic library was constructed in pUC13. Two clones pAC5 and pAC137 hybridising to A. centrale and A. marginale DNA were isolated from this library. One of these, pAC5, also hybridised to DNA from A. ovis. The total insert of pAC5 was subcloned into pBR322. This subclone, pAC5-12, could detect 1 ng A. centrale, 0.5 ng A. marginale and 3.9 ng A. ovis DNA. The hybridisation pattern obtained with pAC5-12 on digests of A. centrale, A. marginale and A. ovis DNA suggests that this probe detects EcoR1 and Hind111-polymorphisms. Probe pAC5-12 could detect A. ovis DNA in 36% of blood samples tested compared to the 33% detectability obtained with microscopy.  相似文献