What is your diagnosis? Chronic bronchointerstitial pneumonia     

Poulsen KP  Marques FJ  Ballegeer EA  McGuirk SM  Dubielzig RR  Peek S 《Journal of the American Veterinary Medical Association》2006,228(1):35-36

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Detection of Toxoplasma gondii antigens reactive with antibodies from serum, amniotic, and allantoic fluids from experimentally infected pregnant ewes     

Marques PX  O'Donovan J  Williams EJ  Gutierrez J  Worrall S  McElroy M  Proctor A  Brady C  Sammin D  Bassett H  Buxton D  Maley S  Markey BK  Nally JE 《Veterinary parasitology》2012,185(2-4):91-100

Toxoplasma gondii, an intracellular protozoan parasite, is one of the major causes of infectious abortion in sheep. To further understand the pathogenesis of toxoplasmosis, serum, amniotic and allantoic fluids and foetal stomach contents were collected from experimentally infected pregnant ewes to determine pathogen numbers and other markers of infection. Fifteen pregnant ewes (90 days of gestation) were each orally inoculated with 3000 sporulated oocysts of T. gondii. Serum samples were collected weekly following challenge. Amniotic and allantoic fluids and foetal stomach contents were collected at 21, 25, 28, 33 and 35 days post-infection. Characteristic placental lesions were detected in 1 of 4 challenged ewes at day 25, 3 of 4 challenged ewes at day 28 and in all challenged ewes at days 33 and 35 post-infection. T. gondii was detected only sporadically in amniotic and allantoic fluids before 35 days of infection, by real-time PCR, and only in ewes with placental lesions. At 35 days post-infection, high numbers of parasite were detected in both amniotic and allantoic fluids. An increase in the number of fluids from challenged animals with IgM and IgG was detected over time, except for IgG in allantoic fluid, which was detected in all samples from day 21 post-infection. IgG in amniotic and allantoic fluids was shown to be specific for T. gondii, and reacted with antigens with an apparent molecular mass of approximately 22 kDa and 30 kDa. Results suggest a maternal source of immunoglobulin in the allantoic fluid and a foetal source of immunoglobulin in the amniotic fluid early in infection but that both sources may contribute immunoglobulin to both fluids at a later stage.  相似文献   

Development of the Campolina foal in Brazil     

H.C. Manso Filho  H.E.C. Costa  F.L. Santos  M.G. Abage  M.C. Ferreira  S.R. Marques   《Journal of Equine Veterinary Science》2000,20(4):275

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Are Pseudokirchneriella subcapitata and Chlorella vulgaris Affected by Environmental Samples from a Rice Field?     

Catarina R. Marques  Nelson Abrantes  Daniela R. de Figueiredo  Mário J. Pereira  Fernando Gonçalves 《Water, air, and soil pollution》2008,189(1-4):49-59

Rice fields represent important production rates in Portugal. However, the intensive soil management and the exploitation of agrochemicals may pose a threat to nontarget organisms. Hence, the present work regards the toxicity screening of surface waters and sediment elutriates collected during the drainage of fields in the vicinity of a rice paddy (Quinta do Seminário, Soure, Portugal): 1. in River Pranto (RP), the river from which the field irrigation water is canalized; 2. inside the rice paddy, from the main drainage channel – Vala de Enxugo (VE). For that purpose, it was used a combination of physico-chemical analyses and bioassays with two green algae species – Pseudokirchneriella subcapitata and Chlorella vulgaris. The chemical screening showed an apparent absence of xenobiotics in sediment samples, while no pesticides were found within the chemical contaminants detected in water samples. The nutrient load reflected low levels of inorganic contamination. Bioassays revealed that P. subcapitata was more sensitive to the overall physico-chemical conditions in natural samples than C. vulgaris, being its growth inhibited under water samples from both sites. On a whole, water samples, mainly those from the main irrigation/drainage channel of the rice fields (VE), were more deleterious to microalgae than those from RP or any of the elutriates.  相似文献   

Supplementary food for Neoseiulus californicus boosts biological control of Tetranychus urticae on strawberry     

Henry E Vacacela Ajila  Felipe Colares  Felipe Lemos  Pedro H Marques  Eliza C Franklin  Willian Santos do Vale  Eugênio E Oliveira  Madelaine Venzon  Angelo Pallini 《Pest management science》2019,75(7):1986-1992

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Soil organic carbon fractions and humic substances are affected by land uses of Caatinga forest in Brazil     

Uemeson José dos Santos  Gustavo Pereira Duda  Marise Conceição Marques  José Romualdo de Sousa Lima  Eduardo Soares de Souza 《Arid Land Research and Management》2019,33(3):255-273

Caatinga is a Brazilian dry ecosystem that occupies around 1 million km² and is one of the largest tropical dry forests of the world. About 46% of the area that was originally covered has been deforested. Land use can cause pronounced reduction in soil carbon stocks that play a major role in the global carbon cycle. The objective of this study was to improve our understanding of the effect of land use on oxidizable carbon fractions, total carbon stocks and humic substances in different layers of soil in a Brazilian semi-arid region. We analyzed soils from tropical dry forest (TDF), forest succession with Anadenanthera falcata (ANA), with Tabebuia alba (TAB), secondary scrubby regeneration (SCR), and non-irrigated maize (MS). Forests showed larger fractions of more labile carbon, except for TDF. The most recalcitrant fraction of carbon stock, humin fraction stock, with the different land use decreased by 38–53% compared to TDF. Oxidizable carbon fractions, carbon stocks, and humic fraction stocks were able to differentiate the successional land uses and agricultural cover from TDF, mainly in the 0–5?cm layer. Our results show that changes in land use, especially with ANA forest succession, showed a larger labile carbon fraction, indicating easy decomposition and loss. Our results provide an alternative tool for the management of deforested areas in tropical dry caatinga ecosystems. This would contribute to the conservation of dry forest systems and could serve as guideline for sustainable management of agriculturally impacted caatinga areas.  相似文献   

Genetic‐Quantitative Study of the First‐Service Pregnancy Probability of Murrah Heifers          下载免费PDF全文

PB Fernandes  KO Marques  FR de Araujo Neto  DP de Oliveira  NA Hurtado‐Lugo  RR Aspilcueta‐Borquis  H Tonhati 《Reproduction in domestic animals》2016,51(3):428-434

Because of the importance of reproduction in stock breeding systems, it is necessary to find selection criteria that increase reproductive efficiency. The aim of this study was to estimate genetic parameters for the probability of conception on first service (PROB) in Murrah heifers, and its association with other traits of economic interest [age at first calving (AFC), service period, calving interval and milk yield at 270 days], with the purpose of evaluating their use as selection criteria. Reproductive information and first lactation records of 1200 Murrah heifers were used to perform two‐trait analyses between PROB and the other characteristics. Bayesian inference was used to estimate the variance components, considering PROB as threshold and the other as linear factors. The results demonstrate that this trait has heritability of 0.15, indicating the possibility of a genetic gain by using it for selection. With respect to the genetic correlation estimates, the only high‐magnitude association was with AFC (?0.899), which is the current criterion indicating sexual precocity of females. In the light of the parameters estimated, the first‐service pregnancy rate is an alternative for indication of sexual precocity, although presenting a smaller genetic gain than the current standard AFC. Nevertheless, additional research should be conducted regarding this trait to assess the economic importance of its use in dairy buffalo production systems.  相似文献   

Effects on erosion of two post-fire management practices: clear-cutting versus non-intervention   总被引：1，自引：0，他引：1  

M. A. Marques  E. Mora 《Soil & Tillage Research》1998,45(3-4):433-439

The different erosion responses derived from two contrasting post-fire management approaches were evaluated: the clear-cutting and immediate removal of the burnt trees and; the non-intervention, that is, leaving the vegetation untouched and the burnt trees standing. Erosion rates were obtained from 200 m² experimental plots, large enough to include the complete processes of rill and inter-rill erosion. Erosion rates of the clear-cut plot were very similar to those of the non-modified plot. Part of the burnt trees was still economically usable. The trees left in situ gradually decayed and were tumbled by the wind; the fire risk incremented. The opening of timber track networks without conservation measures was one of the most important erosion problems associated with clear-cutting. The complementary practice of brushwood dike-construction would need a preliminary study before being applied.  相似文献   

Agricultural diversification reduces the survival period of <Emphasis Type="Italic">Sclerotinia sclerotiorum</Emphasis> sclerotia     

Alexandre Dinnys Roese  Gloria Soriano Vidal  Walmes Marques Zeviani  Anibal de Moraes  Louise Larissa May De Mio 《European journal of plant pathology / European Foundation for Plant Pathology》2018,150(3):713-724

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Depletion study and estimation of withdrawal periods for florfenicol and florfenicol amine in pacu (Piaractus mesopotamicus)          下载免费PDF全文

Tamires Valim Marques  Jonas Augusto Rizzato Paschoal  Rafael Simões Coelho Barone  José Eurico Possebon Cyrino  Susanne Rath 《Aquaculture Research》2018,49(1):111-119

The intensive production of farmed fish is at a global all‐time high, and the control of bacteria proliferation in fish farms requires the frequent use of antimicrobials. This practice raises important environmental concerns related to the emergence of antimicrobial‐resistant bacteria strains. Only a few antimicrobial drugs have been approved for use in aquaculture, one of which is florfenicol. This work studies the depletion and withdrawal period of florfenicol and its main metabolite, florfenicol amine, in pacu (Piaractus mesopotamicus), a neotropical characin widely farmed in the southern hemisphere. Juvenile pacu (average weight of 724 g) were stocked in a closed‐loop laboratory system with controlled water temperature (25.8°C), and were fed for 10 consecutive days with a diet containing an intended dose of 10 mg/florfenicol per kg bw. Muscle and skin tissues were collected at 1, 3, 6, 8, 10, 12 and 16 days post‐treatment, and florfenicol and florfenicol amine were quantified using a validated ultra‐high performance liquid chromatography‐tandem mass spectrometry (UPLC‐MS/MS) method. The limits of quantitation for florfenicol and florfenicol amine were 10 ng/g in muscle and 50 ng/g in skin. Considering a maximum residue limit of 1000 ng/g for the sum of florfenicol and florfenicol amine in muscle with skin in natural proportions a withdrawal period of 5 days (water temperature 25.8°C) or 129 degree days was calculated on the basis of the upper limit of the one‐sided 95% confidence interval for the 99^th percentile derived from the residue depletion study.  相似文献