全文获取类型
收费全文 | 3852篇 |
免费 | 271篇 |
国内免费 | 368篇 |
专业分类
林业 | 286篇 |
农学 | 310篇 |
基础科学 | 242篇 |
618篇 | |
综合类 | 1200篇 |
农作物 | 206篇 |
水产渔业 | 214篇 |
畜牧兽医 | 982篇 |
园艺 | 190篇 |
植物保护 | 243篇 |
出版年
2024年 | 17篇 |
2023年 | 72篇 |
2022年 | 159篇 |
2021年 | 225篇 |
2020年 | 215篇 |
2019年 | 158篇 |
2018年 | 159篇 |
2017年 | 228篇 |
2016年 | 152篇 |
2015年 | 207篇 |
2014年 | 234篇 |
2013年 | 291篇 |
2012年 | 324篇 |
2011年 | 323篇 |
2010年 | 276篇 |
2009年 | 241篇 |
2008年 | 225篇 |
2007年 | 192篇 |
2006年 | 147篇 |
2005年 | 136篇 |
2004年 | 87篇 |
2003年 | 78篇 |
2002年 | 50篇 |
2001年 | 42篇 |
2000年 | 46篇 |
1999年 | 36篇 |
1998年 | 16篇 |
1997年 | 18篇 |
1996年 | 16篇 |
1995年 | 9篇 |
1994年 | 3篇 |
1993年 | 5篇 |
1992年 | 12篇 |
1991年 | 14篇 |
1990年 | 12篇 |
1989年 | 10篇 |
1988年 | 7篇 |
1987年 | 9篇 |
1986年 | 4篇 |
1983年 | 2篇 |
1982年 | 4篇 |
1981年 | 2篇 |
1978年 | 3篇 |
1974年 | 3篇 |
1972年 | 4篇 |
1971年 | 2篇 |
1964年 | 2篇 |
1963年 | 2篇 |
1962年 | 2篇 |
1956年 | 3篇 |
排序方式: 共有4491条查询结果,搜索用时 0 毫秒
71.
The severity of the erosive and ulcerative lesions of the squamous gastric mucosa in 10 standardbred racehorses in training was classified according to a standard scoring system. Each horse was then treated orally for 30 days with 50 g/100 kg bodyweight daily of a pectin-lecithin complex mixed into the feed. At the end of the period of treatment, the gastric lesions were re-evaluated gastroscopically and the scores were compared with those assigned at the previous evaluation. In three of the horses the gastric ulcerations had healed completely, and in six others the lesions had improved significantly. 相似文献
72.
73.
2005年7月,梧州市长州区倒水镇某村猪群暴发以高热、皮肤发红,腹下及四肢下部出现出血斑点为主要症状的疾病.根据临床症状、剖检变化及实验室检验等综合诊断,确诊为猪瘟与猪附红细胞体病混合感染,现报告如下: 相似文献
74.
75.
本试验以辽宁绒山羊和内蒙古绒山羊为实验动物,采用PCR-RFLP技术对-酪蛋白基因(CSN3)片段的Ⅰ的酶切多态性进行分析,结果表明:辽宁绒山羊和内蒙古绒山羊2个山羊群体中均存在CSN3基因Ⅰ酶切位点的多态性,且均有A、B两个等位基因。辽宁绒山羊群体等位基因A和B的基因频率分别为0.46和0.54;内蒙古绒山羊群体中等位基因A和B的基因频率分别为0.31和0.69。CSN3基因Ⅰ酶切位点的基因型分布在辽宁绒山羊群体中极显著偏离Hardy-Weinberg平衡定理(<0.01),在内蒙古绒山羊群体中符合Hardy-Weinberg平衡定理(>0.05),但两个群体间存在显著差异(<0.01)。 相似文献
76.
When the first foci of sharka were discovered in Puglia region (south-east Italy) in the late 1980s, the regional agricultural authorities launched a programme for Plum pox virus (PPV) monitoring and disease eradication. The infecting virus strain was identified as PPV-D. From 1989 to 1993, a strong eradication campaign was successfully carried out involving 13 plum and 2 apricot orchards with different levels of infection. During 1994–2000, besides plum, apricot and peach, monitoring was extended to sweet cherry. At that time, surveys and testing did not reveal any new PPV focus, but the eradication of infected trees continued in a couple of orchards. In 2001–05, particular attention was paid to peach, as devastating PPV-M outbreaks had developed in other areas of the country. A new PPV focus was found in apricot, caused by PPV-Rec, which was promptly eradicated. In the following two years, surveys in the once infected orchard and surrounding peach plantings did not detect any virus spread. The endeavour has taken 15 years making this PPV monitoring and eradication programme the longest in Italy. Its overall results indicate that the fruit tree industry in Puglia region can now be regarded as essentially PPV-free. 相似文献
77.
78.
猪链球菌对红霉素耐药性的研究 总被引:2,自引:0,他引:2
从发病猪体内分离、鉴定猪链球菌,采用肉汤稀释法和纸片琼脂扩散法筛选对红霉素耐药的猪链球菌,用双纸片法确定耐药株的耐药表型,通过聚合酶链反应检测对红霉素耐药的基因ermb/mefA。猪链球菌对红霉素的耐药表型为cMLS表型,即同时对克林霉素耐药。在3株红霉素耐药株中扩增到ermb基因,其余未能检测到ermb或mefA基因。 相似文献
79.
80.
G. La Rosa M. Muscillo A. Di Grazia S. Fontana M. Iaconelli M. Tollis 《Zoonoses and public health》2006,53(6):257-265
Porcine enteroviruses (PEVs) and teschoviruses (PTVs) are described as causative agents of neurological disorders, fertility disorders and dermal lesions of swine. Difficulties in the serological detection of these viruses may lead to a significant underestimation of infections with clinical symptoms. With the recent availability of genome sequence data for all the serotypes, molecular diagnosis is a possibility. The present study describes a new approach to molecular ‘serotyping’ of PTVs and PEV‐B viruses, involving the amplification and sequencing of a genomic fragment of the VP1 coding region. A molecular characterization of Italian entero‐teschovirus isolates was performed using a set of previously published and newly designed polymerase chain reaction primers. A total of 33 porcine isolates and 10 reference strains were analysed. Porcine enterovirus‐B samples were first diagnosed as positive for enterovirus by amplification of the 5′‐non‐translated region. Samples were then typed by amplification and sequencing of a portion of the VP1 coding region. Porcine enterovirus‐A and PTVs were detected by a published assay in the 5′‐NC region that allows them to be differentiated according to the size of amplification product, using the same set of primers. For serotype characterization of PTV, we evaluated four different regions: the N terminus of the capsid protein VP2, the region encoding for RNA‐dependent RNA polymerase, and the capsid VP1 and VP4 regions. The newly designed primers in the VP1 region was proved to be broad in range and suitable for serotype assessment and therefore constitute a useful diagnostic tool for molecular diagnosis of porcine teschovirus/enterovirus strains and for the study of molecular epidemiology and evolution of these viruses. 相似文献