富硒区茶树鲜叶中硒累积与土壤因子的相关性分析   总被引：1，自引：0，他引：1  

张豪杰  郝心愿  周超  王璐  王新超  杨亚军  曾建明  孙冷雪  戴居会  向俊  罗鸿  王朝阳  张贤贵  刘涛 《茶叶科学》2020,40(4):465-477

茶树是富硒植物,饮用富硒茶是一种安全、有效的补硒途径之一。茶叶的硒含量受多种环境因素影响,但有关富硒茶区茶树硒积累特性及主要影响因子的研究还鲜有报道。以高硒茶区湖北恩施、陕西安康不同地点生产茶园成龄茶树和根际土壤为研究对象,结合土壤及植物样品全硒含量等多种指标,明确了根际土壤硒含量对茶树硒分布特性的影响,分析了富硒区土壤pH、硒含量等9个重要土壤特性相关因子的数值分布规律。通过对186组具有代表性的土壤样品和附生茶树新梢组织检测数据进行分组和整体相关性分析,证实了富硒区茶叶全硒含量与土壤硒含量之间存在极显著相关（相关系数r=0.59,P<0.01）,揭示了茶叶全硒含量与土壤有机质含量、水解性氮、锌含量以及茶叶中硫、锌含量的显著相关,同时对安康和恩施地区的土壤和茶叶硒含量相关因素进行了深入分析。提出了茶叶硒含量对土壤有机质含量、硫含量、硒含量和锌含量的数学模型,模型拟合优度为0.512 6,达极显著水平（P<0.01）。  相似文献   

12份美国玉米自交系配合力评价     

冯志前  王博新  徐淑兔  郝引川  张兴华  薛吉全 《玉米科学》2020,28(2):11-17,24

以12份引进美国解禁玉米自交系为供试材料,4份课题组骨干玉米自交系(KA105、KA064、KB043和KB204)为测验种,采用NC-II遗传交配设计,进行配合力测定与分析。结果表明,测验种KA105和KA064产量一般配合力(GCA)效应表现优良,供试美国玉米自交系中PHPR5、MBST、LH213、2FACC、LH212Ht、LH209和PHR47产量GCA效应表现优良。杂交组合PHPR5×KA105、LH213×KA105、PHH93×KA105、2FACC×KA064、LH212Ht×KA105、PHPR5×KB204产量的超标优势较强,可进一步试验。  相似文献   

春季低温对小麦颖果发育的影响     

余徐润  郝朵  顾清钦  倪爱淳  夏敏洁  臧勇  熊飞 《麦类作物学报》2020,40(7):796-805

为了探明春季低温（倒春寒）导致小麦减产的细胞学机理,以扬麦15为材料,在小麦幼穗处于雌雄蕊分化期至药隔期之间时,设置-3 ℃低温处理,通过树脂半薄切片对颖果不同发育时期的形态结构进行观察。结果表明：（1）在颖果发育早期,低温促进了颖果的长度生长,抑制了宽度生长;与对照相比,低温处理组小麦颖果发育进程缩短;（2）低温处理组颖果果皮发育早,中果皮降解速度快;（3）低温处理增加了胚乳淀粉体的积累量,降低了蛋白体的积累量,发育后期胚乳充实度显著低于对照组;（4）与对照相比,低温处理后颖果韧皮部面积和珠心突起传递细胞面积增大。以上结果表明,春季低温处理更有利于养分运输组织的发育,使得灌浆时间缩短,最终导致淀粉积累量增加及颖果早熟。  相似文献   

生物菌肥在西瓜上的施用效果     

李秀启  尹国红  郝浩浩  贾宝华  张庆社 《长江蔬菜》2020,(4):71-73

以西瓜品种豫艺甜宝为试材,研究了生物菌肥在西瓜上的施用效果。试验结果表明,生物菌肥施用可以提前西瓜的物候期、提高叶片的光合速率和促进植株生长,每1 hm2施有机无机复混肥1800 kg+有机菌肥2250 kg和三元复合肥750 kg+有机菌肥2250 kg 2个菌肥处理分别比常规施肥增产19.48%、36.53%。与常规施肥相比,生物菌肥还可有效增加西瓜含糖量,提升品质。综合考虑各项指标,在本试验条件下,每1 hm^2施有机无机复混肥1800 kg+有机菌肥2250 kg的施肥处理为最优施肥方案。  相似文献   

玉米粉淀粉含量近红外模型建立与优化     

韩洁楠  王美娟  赵训超  鲁鑫  周志强  李明顺  张德贵  郝转芳  翁建峰  雍洪军  李新海 《玉米科学》2020,28(6):81-87

以230份玉米自交系为样本,采用旋光法与一阶导数及去一条直线的光谱预处理法,构建玉米粉样淀粉含量的近红外分析（NIRS）模型。研究标明,该模型可显著提高子粒淀粉含量预测的准确性。该模型的定标标准偏差（RMSEE）、交叉验证标准偏差（RMSECV）、外部验证标准偏差（RMSEP）、定标相关系数（R_cal²）、交叉验证相关系数（R_cv²）、外部验证相关系数（R_cv²）分别为0.609、0.722、0.738、0.909、0.864和0.854。建立的玉米粉样NIRS模型可将预测值与化学值偏差控制在1.7%内,能够准确定量分析玉米子粒淀粉含量,应用于育种材料早期筛选及群体水平粗淀粉分析。  相似文献   

Role of IP₃R in LH-EGFR-induced mouse oocyte meiotic resumption     

HAO Xiao-qiong  LI Yan-kang  FU Xu-yang  JIA Fang-yi 《园艺学报》2020,36(3):532-538

AIM: To explore the effect of inositol 1, 4, 5-trisposphate receptor (IP₃R) in luteinizing hormone-epidermal growth factor receptor (LH-EGFR)-induced oocyte meiotic resumption. METHODS: Models of mouse cumulus-oocyte complexs (COCs) culture and follicle culture in vitro were generated to study the effects of 2-aminoethyl diphenyl borate (2-APB) and heparin (IP₃R specific inhibitors) on LH/EGF-induced oocyte meiotic resumption and EGF-induced cumulus cell expansion. Real-time PCR was used to detect the mRNA expression of cumulus expansion-related factors. The changes of the intracellular calcium level were monitored using Fluo 3-AM, and the cGMP level was measured by ELISA. RESULTS: The inhibitors of IP₃R, 2-APB and heparin, dramatically reversed EGF-induced oocyte maturation (P<0.05) and decreased cGMP levels in COCs (P<0.05). In addition, 2-APB and heparin reversed EGF-induced cumulus expansion, and significantly inhibited EGF-induced cumulus expansion-related factor expression (P<0.05). The activation of IP₃R increased intracellular calcium level, and the study found that 2-APB and heparin dramatically reversed EGF-induced elevation of calcium level in cumulus cells (P<0.05). Follicular culture in vitro showed that 2-APB and heparin significantly reversed the LH-induced oocyte maturation (P<0.05). CONCLUSION: LH-EGFR signaling pathway increases calcium level in cumulus cells through IP₃R, resulting in meiotic resumption.  相似文献   

Effects of cardiac contractility modulation on ventricular electrical remodeling in rabbits with chronic heart failure     

ZHANG Fei-fei  Zhao Shi-xia  HAO Qing-qing  Li Rong  LI Ying-xiao  Dang yi  QI Xiao-yong 《园艺学报》2020,36(3):547-551

AIM: To investigate the effects of cardiac contractility modulation (CCM) on ventricular electrical remodeling in a rabbit model of chronic heart failure. METHODS: The rabbits were divided into sham group, heart failure(HF) group and HF+ CCM group. The rabbit model of chronic heart failure was established by ligating the ascending aortic root. Then electrical stimulations during the absolute refractory period were delivered lasting 6 h everyday for 4 weeks in rabbits of HF+ CCM group. The QTc and ventricular effective refrective period (VERP) were recorded. The protein and mRNA levels of Kv1.4, Kv4.3 and connexin 43 (Cx43) were determined by Western blot and RT-qPCR. RESULTS: Compared with sham group, QTc were significantly prolonged in HF rabbits at week 12 (P<0.05). CCM therapy shortened QTc of rabbits with heart failure at week 16 (P<0.05). Compared with sham group, VERP significantly increased in HF group and HF+ CCM group, while CCM therapy shortened VERP of rabbits with heart failure at week 16 (P<0.05). Compared with sham group, the mRNA and protein levels of Kv1.4, Kv4.3 and Cx43 were decreased in HF group and HF+ CCM group (P<0.05). However, CCM therapy restored the mRNA and protein levels of Kv1.4, Kv4.3 and Cx43 of rabbits with heart failure (P<0.05). CONCLUSION: CCM suppresses ventricular electrical remodeling in heart failure and the underlying mechanism may be associated with increasing Kv1.4, Kv4.3 and Cx43 expression.  相似文献   

mTOR kinase inhibitor CC-223 suppresses human breast cancer cell viability     

GUO Shu-xin  ZHOU Xiao  PAN Xiao-xuan  Xu Hao  HAO Gang-ping  WANG Feng-ze 《园艺学报》2020,36(2):234-238

AIM: To investigate the inhibitory effect of CC-223, an inhibitor of mammalian target of rapamycin (mTOR) kinase, on the viability of human breast cancer cells and its mechanism. METHODS: The inhibitory effect of CC-223 on the viability of MCF-7 cells and MDA-MB-231 cells was measured by CCK-8 assay. The cell cycle distribution of breast cancer cells was examined by flow cytometry. The expression of cell cycle-related proteins and oncoproteins c-Myc and survivin was analyzed by Western blot. RESULTS: CC-223 significantly inhibited the viability of both MCF-7 and MDA-MB-231 cells (P<0.05). CC-223 induced cell cycle arrest in both G₁ phase and G₂/M phase in the MCF-7 cells (P<0.05). However, low concentration of CC-223 treatment resulted in the accumulation of MDA-MB-231 cell cycle in the G₂/M phase, and the cell number in G₁ phase was unaffected. Treatment with CC-223 for 24 h clearly inhibited the protein levels of cyclin B1, cyclin D1 and phosphorylated cell division cycle protein 2 in the breast cancer cells (P<0.05). CC-223 suppressed the expression of c-Myc and survivin in both MCF-7 cells and MDA-MB-231 cells (P<0.05). CONCLUSION: CC-223 inhibits cell viability by blocking cell cycle progression and down-regulating expression of c-Myc and survivin in both MCF-7 and MDA-MB-231 cells.  相似文献   

4种杀菌剂防治草坪草夏季斑枯病研究     

韩惠兰  李迎宾  额尔敦阿古拉  郝志刚  罗来鑫  李健强 《植物保护》2020,46(5):298-302

夏季斑枯病是危害冷季型草坪草的主要病害之一,严重影响草坪质量,目前我国仍未有登记药剂防治夏季斑枯病。本研究结合室内毒力测定及田间防效检验,分析比较了嘧菌酯、稻瘟灵、丙环唑和甲基硫菌灵4种杀菌剂对夏季斑枯病菌的抑制效果,评估了药剂的田间防病效果。结果表明,丙环唑对供试的夏季斑枯病菌3株靶标菌抑制效果最佳,EC50为0.09~0.16 μg/mL;嘧菌酯次之,EC50为0.14~0.28 μg/mL;稻瘟灵和甲基硫菌灵抑制效果再次之。4种杀菌剂对夏季斑枯病害均具有较好的防控效果;其中50%嘧菌酯水分散粒剂制剂用量800 g/hm2防控效果最佳,第4次药后17 d可达99.09%;25%丙环唑乳油制剂用量2 000 g/hm2防控效果次之,第4次药后17 d为93.14%。  相似文献   

解淀粉芽胞杆菌菌株HRH317对感染串珠镰孢菌玉米幼苗伏马毒素B₁含量及相关防御酶活性的影响     

赵欣  郝林 《植物保护学报》2020,47(2):273-282

为探讨解淀粉芽胞杆菌Bacillus amyloliquefaciens菌株HRH317对感染串珠镰孢菌Fusarium moniliforme玉米幼苗产生伏马毒素B_1(FB_1)的影响,采用牛津杯法测定菌株HRH317对串珠镰孢菌的抑制活性,并通过浸种处理进行盆栽试验,应用高效液相色谱技术对生长至3叶期后不同时间玉米幼苗叶片中FB_1含量进行测定,同时于室内测定玉米幼苗叶片防御酶超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、苯丙氨酸解氨酶(PAL)和过氧化物酶(POD)的活性。结果表明:解淀粉芽胞杆菌菌株HRH317能明显抑制串珠镰孢菌生长,抑菌圈直径平均可达33.31 mm;玉米幼苗生长至3叶期后1～6 d,菌株HRH317能有效抑制玉米植株体内FB_1含量,经串珠镰孢菌分生孢子悬浮液与菌株HRH317菌悬液1∶1混合液处理玉米种子后,对幼苗中FB_1的抑制率为59.20%～75.70%;而玉米种子先接种菌株HRH317菌悬液后接种串珠镰孢菌分生孢子悬浮液处理对幼苗中FB_1的抑制率为76.77%～88.10%。且这2种处理中幼苗叶片的SOD、CAT、PAL和POD活性均较对照有不同程度提高,其峰值是对照的1.24～5.45倍。表明解淀粉芽胞杆菌菌株HRH317可通过抑制FB_1产生来降低串珠镰孢菌对玉米幼苗的侵害,同时能诱导玉米植株体内防御酶活性的表达而增强其系统抗性,在防治玉米穗腐病方面具有潜在的应用价值。  相似文献