Autosomic 27 Trisomy in a Standardbred Colt     

Leonardo F.C. Brito DVM  PhD  Patricia L. Sertich MS  VMD  Keith Durkin BSc  Bhanu P. Chowdhary BVSc&#x;AH  PhD  Regina M. Turner VMD  PhD  Lauren M. Greene VMD  Sue McDonnell PhD 《Journal of Equine Veterinary Science》2008,28(7):431-436

A 19-month-old Standardbred colt was donated to the University of Pennsylvania School of Veterinary Medicine with a suspicion of intersexuality. The anal−genital distance and penis were normal, and there was no evidence of intersexuality, but the colt was bilaterally cryptorchid. Several aspects of the colt's behavior appeared unusual, including general temperament and behavior described as sympathetically dull and affable. With herd mates, the colt appeared slow to perceive or to learn the usual intraspecies social cues. An atypical gait characterized by intermittent unnatural shuffle of the hind limbs, sliding them along in short rhythmic strides for 3 to 10 seconds at a time was noted at times when a horse might normally transition from a slow walk to a fast walk or a slow trot. Occasionally the colt exhibited slight protrusion of the tongue through the teeth and lips with jaw movements and smacking of the tongue against the teeth as if struggling to retract the tongue to the normal position. Evaluation of the karyotype combined with fluorescent in situ hybridization (FISH) revealed an abnormal male karyotype showing trisomy of chromosome 27 (65, XY + 27). The colt was euthanized at 24 months of age, and a necropsy revealed no significant abnormalities. This case of trisomy was not associated with developmental abnormalities described in other rare reports of trisomy in horses; however, some features were strikingly similar to that of humans with trisomy 21. FISH was demonstrated to be an excellent method for correct identification of equine chromosomes.  相似文献   

Prevalence of Multi-Drug-Resistant Salmonella in Equids Maintained by Low Income Individuals and on Designated Equine Farms in India     

B.R. Singh PhD  N. Babu MVSc  J. Jyoti MVSc  H. Shankar PhD  T.V. Vijo BVSc  AH  R.K. Agrawal PhD  M. Chandra PhD  D. Kumar MVSc  A. Teewari MVSc 《Journal of Equine Veterinary Science》2007,27(6):266-276

We examined 872 equids (445 maintained by low-income individuals and 427 maintained on nine designated equine farms) and, using previously described methods for bacteria, isolated Salmonella from fecal samples of 59 (6.77%) animals. Of the 646 horses, 183 donkeys, and 43 mules that had feces cultured for Salmonella, 42 (6.5%), 7 (3.8%), and 10 (23.3%), respectively, were excreting Salmonella strains in feces. Six horse mares were excreting Salmonella enterica of two different serovars simultaneously. A total of 65 Salmonella enterica isolates belonged to 13 serovars, namely S. paratyphi B var Java (14), S. I. 4, 5, 12, 27: r, i: 1, 5 (11), S. Drogana (8), S. Newport (7), S. Saintpaul (5), S. Lagos (4), S. Typhimurium (5), S. Kottbus (3), S. Bovismorbificans (3), S. Dumfries (2), S. Tshiongwe (1) S. Weltevreden (monophasic) (1), and S. enterica ssp salamae (1). With Salmonella-specific polymerase chain reaction (PCR) using hisJ gene primers, 107 (12.3) fecal samples yielded a specific amplicon of 496 bp. On using PCR, prevalence of Salmonella in donkeys, horses, and mules was 4.9%, 10.8%, and 65.1%, respectively. With both methods of Salmonella detection in feces, prevalence was significantly higher in female than in male donkeys and horses. Salmonella shedding in feces was significantly higher in equids maintained by low-income people than those at designated equine farms. Almost all Salmonella isolates (63 of 65) had multiple-drug-resistance (MDR, resistance to three or more drugs). Salmonella isolates were commonly resistant to sulfamethoxazole (90.8%), tetracycline (70.8%), doxycycline (67.7%), furazolidone (66.2%), and colistin (55.4%). A few isolates had resistance to trimethoprim (3.1%), ciprofloxacin (3.1%), ceftriaxone (3.1%), ceftazidime (3.1%), cefoperazone (3.1%), chloramphenicol (4.6%), cefotaxime (6.2%), gentamicin (9.2%), ampicillin + cloxacillin (9.2%), cotrimoxazole (13.8%), kanamycin (13.8%), amoxicillin + clavulanic acid (16.9%), imipenem (16.9%), ampicillin (18.5%), amikacin (23.1%), neomycin (27.7%), nalidixic acid (33.8%), and streptomycin (36.9%). With the exception of 13 Salmonella isolates of S. Drogana (4), S. Newport (4), S. I. 4, 5, 12, 27: r, i: 1, 5 (4) and S. Kottbus (1) serovars, all had one or more than one plasmid. Molecular weight of plasmids ranged between 3 kDa and >87 kDa. One heavy plasmid (≥87 kda) was present in all the 52 plasmid-positive strains. Presence of plasmid could not be correlated with MDR in Salmonella isolates from equids.  相似文献   

Fertility Results of Artificial Inseminations Performed with Liquid Boar Semen Stored in X-Cell^TM vs BTS Extender     

T Haugan  AH Gaustad  O Reksen  YT Gröhn  PO Hofmo 《Reproduction in domestic animals》2007,42(1):94-99

The objective of the present field study was to compare the fertility results for boar semen diluted in X-cell stored up to 4-5 days before artificial insemination (AI) with semen diluted in Beltsville thawing solution (BTS) used for AI following 2-3 days of storage (where the first day being the collection day). A total number of 2601 double inseminations in Norwegian herds were included in this two-trial study. All the boars used in the study were mature cross-bred Norwegian Landrace x Duroc (LD), which were routinely used for AI in Norway. The inseminated gilts and sows were Norwegian Landrace x Yorkshire (LY). The AI doses contained 2.5 billion spermatozoa, and consisted of a mixture of semen from three, occasionally four, boars (i.e. heterospermic semen). Fertility was measured in terms of the likelihood of farrowing and subsequent litter size. The fertility of the semen in both of the extenders was satisfactory and no significant differences were found either in semen stored 4-5 days in X-cell compared with 2-3 days in BTS or in semen stored 2-3 days in X-cell compared with 2-3 days in BTS. The storage capability findings for the long-term extender X-cell could significantly simplify the practical issues of semen production and the distribution of AI doses containing 2.5 billion spermatozoa. However, in pig production systems where all semen is used within 2-3 days, the short-term extender BTS is as good as the more expensive extender X-cell.  相似文献   

内蒙古阿拉善荒漠啮齿动物群落结构及其多样性研究   总被引：6，自引：0，他引：6  

武晓东  阿娟  付和平 《草地学报》2003,11(4):312-316

对内蒙古阿拉善荒漠区啮齿动物群落结构和多样性进行了研究。依据样地种多度(捕获率)聚类方法,并结合生境中地带性植被类型的综合特征,该荒漠区啮齿动物可划分为6个地带性群落。Ⅰ.小毛足鼠(Phodopus roborovskii)+三趾跳鼠(Dipus sagitta)+子午沙鼠(Meriones meridianus)群落。Ⅱ.长尾仓鼠(Cricetulus longicaudatus)+大林姬鼠(Apodemus speciosus)群落。Ⅲ.五趾跳鼠(Allactaga sibirica)+子午沙鼠(Meriones meridianus)+三趾跳鼠(Dipus sagitta)群落。Ⅳ.三趾跳鼠(Dipus sagitta)+小毛足鼠(Phodopus roborovskii)+子午沙鼠(Meriones meridianus)群落。Ⅴ.长耳跳鼠(Euchoreutes naso)+五趾跳鼠(Allactaga sibirica)群落。Ⅵ.子午沙鼠(Meriones meridianus)+黑线仓鼠(Cricetulus barabensis)群落。其中群落Ⅲ多样性指数最高,为2.0557;群落I与群落IV的相似性系数最大,为0.5147;群落Ⅲ具有代表性。该荒漠区啮齿类数量与生境中的土壤水分、土壤基质呈显著正相关;与建群植物种数呈显著负相关;而与生境的海拔高度相关性极低。  相似文献   

Effects of different concentrations of Chir98014 as an activator of Wnt/beta-catenin signaling pathway on oocyte in-vitro maturation and subsequent embryonic development in Sanjabi ewes     

Sarah Samereh  Hadi Hajarian  Hamed Karamishabankareh  Leila Soltani  Saheb Foroutanifar 《Reproduction in domestic animals》2021,56(7):965-971

The present study was conducted to investigate the effects of the activator factor of the WNT pathway, chir98014, leading to the in vitro sheep oocyte maturation medium, on the cumulus cell development, different nuclear maturation stages and the following process of embryonic development. Experiments included (a) addition of different concentrations (0, 0.1, 0.5, 1 µm) of chir98014 to the maturation medium and evaluation of the cumulus cell expansion, (b) addition of different concentrations of chir98014 to the maturation medium and investigation of different nuclear maturation stages, (c) addition of different concentrations of chir98014 to the maturation medium and examination of the subsequent embryonic maturation process and (d) addition of different concentrations of chir98014 to the embryonic development culture medium (the first 48 hr) and investigation of the subsequent embryonic development process. The extracted data were analysed using the SPSS software, considering the significance level of p < .05 and making the mean comparisons. The results showed that the addition of the 0.1 µM concentration of chir98014 to the maturation medium had no significant effects on the oocyte maturation and embryo development post-fertilization but it enhanced the Cumulus-oocyte complexes (COCs) expansion. In the fourth experiment, the low concentration of chir98014 in the embryo culture media improved the embryo development process, whereas the high one had a detrimental effect on it, as compared to the control group. Thus, the presence of the lower concentrations of this compound in the embryonic culture medium had favourable effects on the development of embryos.  相似文献   

Marking to weaning production aspects of lambs provided with NSAID analgesia compared with lambs receiving no analgesia at the time of elastrator ring marking     

AH Small  S Belson  H Brewer  SM Schmoelzl 《Australian veterinary journal》2021,99(1-2):40-43

The provision of analgesia at the time of marking has been adopted by the Australian sheep industry, but data on production benefits are lacking. In the current study, alternate lambs were provided with either meloxicam (non‐steroidal anti‐inflammatory drug [NSAID], n = 781) or no analgesia (NONE, n = 822) at the time of ring castration and tail docking. Six distinct management groups of lambs were studied. Lambs were weighed immediately before marking and then again at weaning. There was no significant effect of treatment on average daily gain between marking and weaning in cross‐bred lambs. In Merino lambs, average daily gain was 5 g/day lower (P < 0.005) in lambs receiving NSAID, but this may not be biologically significant. Lamb losses were significantly (P < 0.05) lower in the NSAID group (1.1%) than in the NONE group (2.7%). This observation is worth validating in larger studies, particularly considering that lamb mortality is a significant cost to production and welfare concern.  相似文献   

Influence of orthopedic examination on lameness scores and interobserver and intraobserver agreement in dogs with naturally occurring elbow osteoarthritis     

Karanvir S. Aulakh BVSc & AH  MS  MS  CCRP  DACVS-SA  Kaustubh R. Dongaonkar BVSc & AH  MVSc  Katherine Barnes DVM  MS  CCRP  DACVS-SA  Alberto J. Gines DVM  DECVS  Jude T. Bordelon DVM  MS  MBA  DACVS-SA  Don Hulse DVM  DACVS  DECVS  Harmeet K. Aulakh BVSc & AH  MVSc  DACVIM  Chin-Chi Liu MS  MApStat  PhD 《Veterinary surgery : VS》2020,49(3):455-462

  相似文献   

Effect of sedation or general anesthesia on elbow goniometry and thoracic limb circumference measurements in dogs with naturally occurring elbow osteoarthritis     

Emily Clarke DVM  Karanvir S. Aulakh BVSc & AH  MS  MS  CCRP  DACVS-SA  Caleb Hudson DVM  MS  DACVS-SA  Katherine Barnes DVM  MS  CCRP  DACVS-SA  J. Alberto Gines DVM  DECVS  Chin-Chi Liu MS  MApStat  PhD  Harmeet K. Aulakh BVSc & AH  MVSc  DACVIM 《Veterinary surgery : VS》2020,49(7):1428-1436

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Sperm motility and fertilizing ability in the Persian sturgeon Acipenser persicus     

Sayyed Mohammad Hadi Alavi  & Jacky Cosson 《Aquaculture Research》2005,36(9):841-850

The motility and fertilizing ability of the Persian sturgeon, Acipenser persicus, spermatozoa were investigated. Optimum ionic content (Na⁺, K⁺, Ca²⁺ and Mg²⁺) and pH of activation solution as well as the optimum dilution rate were determined. The results show optimum motility characteristics of spermatozoa in buffered solutions containing 25, 0.2, 3 and 10 mM L^?1 Na⁺, K⁺, Ca²⁺ and Mg²⁺, respectively, at dilution rate 1:50 and pH 8.0. To test the fertilizing ability of sperm, two buffered saline solutions were used as activation solution of sperm motility. The present study indicated (1) spermatozoa motility is one of key factors that influence on fertilizing ability of sperm, (2) a high fertilizing ability of sperm is obtained after dilution in saline solutions rather than in freshwater and (3) a maximum fertilization rate occurs in buffered saline solution containing 0.2 mM L^?1 K⁺. There is also a good correlation between biochemical characteristics of seminal plasma and fertilizing ability of sperm.  相似文献   

Equine neural angiostrongylosis     

JD WRIGHT  WR KELLY†  J. HAMILTON  AH WADELL† 《Australian veterinary journal》1991,68(2):58-60

Verminous encephalomyelitis due to Angiostrongylus cantonensis larvae was diagnosed in 2 foals at necropsy. The principal clinical feature was tetraparesis, although history and neurological examination revealed progressive and multifocal neurological disease. At presentation, a tentative diagnosis of parasitic larval migration involving the central nervous system (CNS), presumably due to Strongylus vulgaris, was proposed. Dissection of the spinal cord in one case resulted in recovery of intact larvae of both sexes of A. cantonensis. In both foals, histopathology of the brain and spinal cord revealed nematode sections which were consistent with A. cantonensis larvae.  相似文献