First report of Faba bean necrotic yellows virus (FBNYV) infecting chickpea (Cicer arietinum) and faba bean (Vicia faba) crops in Sudan     

K. M. Makkouk  A. A. Hamed  M. Hussein  S. G. Kumari 《Plant pathology》2003,52(3):412-412

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The effects of stocking density on hemato-immunological and serum biochemical parameters of rainbow trout (Oncorhynchus mykiss)     

Peyman Yarahmadi  Hamed Kolangi Miandare  Seyed Hossein Hoseinifar  Nahid Gheysvandi  Arash Akbarzadeh 《Aquaculture International》2015,23(1):55-63

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Recovery of sperms bearing X chromosomes with different concentrations of magnetic nanoparticles in ram     

Maryam Moradi  Hadi Hajarian  Hamed Karamishabankareh  Leila Soltani  Bijan Soleymani 《Reproduction in domestic animals》2021,56(2):263-269

Pre-conceptual sex selection is still a highly debatable process whereby X and Y chromosome bearing spermatozoa are isolated before oocyte fertilization. Recently, magnetic nanoparticles (MNP) have been used to determine X and Y chromosomes bearing spermatozoa as a result of searching for a cheap, highly efficient method using non-toxic materials. This study aimed to recover the sperm bearing X chromosomes in ram with different concentrations of MNP and then evaluate the success of this method using polymerase chain reaction (PCR). Ram sperms were divided into four groups, treated with 0 (control), 50, 100 and 200 μg/ml MNP, respectively. MNP was used to restore sperm cells bearing X chromosomes. Upon recovery, the PCR was performed to identify the X and Y sperms, Methyl ThiazoleTetrazolium (MTT), to assess MNP toxicity and sperm viability and acridine orange (AO) to evaluate sperm DNA integrity. The results of PCR revealed that the treatment of spermatozoa- bearing X chromosomes with 50 μg/ml MNP had the highest effects on the recovery of X sperm rather than the other concentrations of MNP. However, the concentrations of MNP did not have any toxic effects on spermatozoa, sperm viability and, DNA integrity, but the high concentration of MNP (200 μg/ml) significantly reduced DNA integrity. According to MTT and AO results, the concentrations of MNP used in this study had no toxic effects on spermatozoa and did not reduce the sperm viability and DNA integrity, except that 200 μg/ml MNP significantly reduced DNA integrity.  相似文献   

Bioactive constituents of Leptadenia arborea     

El-Hassan A  El-Sayed M  Hamed AI  Rhee IK  Ahmed AA  Zeller KP  Verpoorte R 《Fitoterapia》2003,74(1-2):184-187

The aerial part of Leptadenia arborea has been shown to contain pinoresinol (1), syringaresinol (2), leucanthemitol (3) and E-ferulaldehyde (4). These known compounds are being reported for the first time from this plant. Among them, syringaresinol has shown an inhibitory effect against acetylcholinesterase. The IC(50) (the concentration of 50% enzyme inhibition) value of this compound was 200 microg/ml.  相似文献   

Glutathione and its Related Enzymes in the Nile Fish   总被引：2，自引：0，他引：2  

Ragaa R. Hamed  Tahany M. Maharem  Rasha A. M. Guinidi 《Fish physiology and biochemistry》2004,30(3-4):189-199

Glutathione (GSH) and related enzymes, glutathione transferase (GST), glutathione peroxidase (GPx) and glutathione reductase (GR) form an important phase 2 biotransformation enzymes system. The objective of this study was to compare this enzymes system in three fish species from the river Nile, Oreochromis niloticus, Claris lazera and Cyprinus carpio in order to establish the main differences and to purify and characterize GST from the liver of O. niloticus.The level of GSH and the activity of GST, GPx and GR in the liver, kidney and gills of the three fish species were examined. A simple reproducible procedure for the purification of GST from the liver of O. niloticus to homogeneity, which includes chromatography on DEAE- cellulose followed by affinity chromatography on GSH-sepharose was established. The molecular mass was found to be 25,460 Da by SDS-PAGE. The Michaelis-Meneten constants (K_m) of the enzyme for GSH and CDNB were 0.35 mM and 0.42 mM, respectively. The affinity purified enzyme exhibited maximum pH at pH 8.0 and increasing pH above 8.0 did not affect the observed maximum. The purified enzyme acts readily on CDNB, less readily on some standard transferase substrates (1,2-dichloro-4-nitrobenzene and p-nitrophenethyl bromide) and not at all on others (bromosulphophthalein and p-nitrobenzyl chloride). Bromosulfophthalein, cibacron blue and hematin inhibited CDNB-conjugating activity of the purified enzyme with IC₅₀ 0.079, 3.98 and 0.126 μM, respectively.  相似文献   

Soil Salinization in Some Irrigated Areas of the Kingdom of Bahrain     

Asma Ali Abahussain  Abdelhadi Abdelwahab Mohamed  Ahmed Ali Salih  Ahmad Al Safe  Nader Abdul Hamed Mosa  Yahya Othman 《农业科学与技术》2014,(2):112-122

Monitoring and assessment of agricultural land degradation is of vital importance for better land and water management planning and reclamation. It requires setting baseline information and basic analysis at specific time and space. About 33 geo-referenced soil sampling spots were selected in two agricultural production locations in the Kingdom of Bahrain to assess the status and preliminary causes of land degradation. Soil samples were taken from 13 sites in Diraz location while 19 samples were taken from Budayyi location. The samples were taken to 90 cm depth at 30 cm intervals. Standard procedures were followed to determine soil physiochemical properties. In addition, field observations on farm condition, distance from the sea, method of irrigation and irrigation water source were taken. Some of the soil samples were deliberately taken from outside the irrigated basins among trees compared with samples taken from inside the actively growing area for comparison. The results indicated that the salinity level was significantly （P 〈 0.001） higher at the 0-30 cm soil depth compared with 30-60 cm or 60-90 cm depths in both locations. The distance from the sea did not show clear correlation with surface soil salinity in Budayyi area compared with Diraz. Both locations showed significantly higher salinity levels on samples taken outside the actively growing areas compared with those taken from within. The effect is more prominent at the 0-30 cm depth. The observed variability on salinity levels may be attributed to farm management practices and deteriorating quality of ground water. Thus, agricultural land degradation in Bahrain cannot be attributed to ground water deterioration alone. The use of tertiary treated sewage water （TSE） may ease the pressure on ground water, but the pH of the TSE should be carefully monitored and managed with proper studies on leaching requirements to avoid further salinity complications.  相似文献   

New molecular markers linked to qualitative and quantitative powdery mildew and scald resistance genes in barley for dry areas     

Haitham Sayed  Gunter Backes  Hamed Kayyal  Amor Yahyaoui  Salvatore Ceccarelli  Stefania Grando  Ahmad Jahoor  Michael Baum 《Euphytica》2004,135(2):225-228

A partial genetic linkage map was constructed on 71 doubled-haploid lines derived from a cross between the barley lines Tadmor and WI2291 with 181 molecular markers. The segregating population was used to detect markers linked to the gene Mlg conferring resistance to powdery mildew (Erysiphe graminis f. sp. hordei) and to genes for quantitative resistance to scald (Rhynchosporium secalis). The gene Mlg on chromosome 4H was flanked by two AFLP markers at a distance of 2.0 and 2.4 cM, respectively. QTLs for resistance to scald were detected on chromosomes 2H and 3H. This association of molecular markers with qualitative and quantitative disease resistance loci represents a valuable starting-point for marker-assisted selection. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Erratum to: Genetic variation of stem characters in wheat and their relation to kernel weight under drought and heat stresses     

Ahmed Sallam  Mervat Hashad  El-Sayed Hamed  Mohamed 《Journal of Crop Science and Biotechnology》2015,18(5):361-361

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Segregation distortion in doubled haploid lines of barley (Hordeum vulgare L.) detected by simple sequence repeat (SSR) markers     

Haitham Sayed  Hamed Kayyal  Luke Ramsey  Salvatore Ceccarelli  Michael Baum 《Euphytica》2002,125(2):265-272

A total of 147 simple sequence repeat (SSR) markers (including86 barley and 61 wheat microsatellite markers) were tested for their segregation in a doubled haploid (DH) and an F₂ population of barley. The DH population consisted of 71 doubled haploid lines, developed from F₁ plants of a cross between Tadmor and WI2291using isolated microspore culture technique. A genetic linkage map consisting of 43 microsatellite markers was constructed using the DH population. Particularly on chromosome 4H microsatellite markers showed distorted segregation ratios. Segregation of DH lines based on molecular markers were compared with segregation of 92 F₂ lines from the same cross. The proportion of loci deviating from the expected monogenic segregation ratios in the DH population was significantly higher (19/43loci, 44%) than in the F₂ population (7/43 loci, 16%). The deviation was biased towards the WI2291 parent alleles. In line with this observation, WI2291 was found to perform better than Tadmor in regenerating green plantlets with the isolated microspore-culture technique. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

批式与两相高温厌氧消化厨余和杂草废弃物对比研究   总被引：4，自引：1，他引：4       下载免费PDF全文

刘广青  张瑞红  董仁杰 《中国农业大学学报》2006,11(6):111-115

以厨余和杂草废弃物混合物为发酵底物进行了批式和两相厌氧发酵试验。经过25 d的批式厌氧消化后,污染负荷(以挥发固体(VS)质量浓度计)为6.5、12.5、16.0和20.0 g/L的沼气产率分别为1 012、863、879和467mL/g,甲烷产率分别为595、442、440和316 mL/g,其中接种污泥对沼气产率和甲烷产率的贡献分别是304和170mL/g。试验结果表明:污染负荷为6.5 g/L时达到最大产气效率,80%的气体在反应的前8 d产生;污染负荷为12.5和16.0 g/L的产气率在统计学上无显著差别;污染负荷在20.0 g/L时出现产甲烷抑制,甲烷体积分数在反应的前2 d为20%左右。两相发酵试验采用厌氧固体床反应系统,包括4个1 L的固体床反应器和1个2.2 L的厌氧批式反应器。经过12 d的消化,沼气和甲烷产率分别为530和351 mL/g,系统总固体(TS)和VS去除率分别为78%和82%。与批式消化比较,该两相系统污染负荷高,产气稳定,周期短,是处理该类型有机废弃物的有效方法。  相似文献