Immunohistochemistry Diagnosis of an Ovarian Dysgerminoma in One Bitch     

JK Park  MJ Goo  IH Hong  MR Ki  JY Han  KS Jeong 《Reproduction in domestic animals》2009,44(5):855-858

An ovarian enlargement (diameter, 8 cm) was identified and surgically excised from a 5-year-old female dog. Microscopic examination of the multinodular neoplasm revealed sheets of polygonal neoplastic cells with large nuclei, frequent mitosis, necrosis and haemorrhage. Immunohistochemically, the neoplastic cells were positive for vimentin and alkaline phosphatase but were negative for CD3, CD79a, cytokeratin, alpha-fetoprotein, inhibin-α and S-100. The histopathological diagnosis of the mass was unilateral ovarian dysgerminoma.  相似文献   

Modelling the predisposition of Norway spruce to <Emphasis Type="Italic">Ips typographus</Emphasis> L. infestation by means of environmental factors in southern Finland     

Minna Blomqvist  Maiju Kosunen  Mike Starr  Tuula Kantola  Markus Holopainen  Päivi Lyytikäinen-Saarenmaa 《European Journal of Forest Research》2018,137(5):675-691

New measures for effective monitoring and controlling of bark beetle infestations are needed as a response to intensified outbreaks caused by the climate change. Various environmental factors affect tree health and susceptibility, as well as stand predisposition to bark beetles. European spruce bark beetle Ips typographus L. abundance and outbreak frequency in Finland has significantly increased during the last decade. The ability to identify sites under a high risk of infestation would facilitate adaptation to this new situation and help target limited forest health management resources. Accordingly, our goal was to investigate the importance of various stand, soil and topographic characteristics in the assessing predisposition of Norway spruce dominated urban forest in southern Finland to I. typographus infestations. Information on the environmental factors was assessed in the field in 2014 and derived from a digital elevation model. Ips typographus infestation intensity was classified into three infestation index classes based on tree-wise symptoms of resin flow, discoloration and defoliation. Cumulative logit link models were utilized for investigating stand-level infestation probability. The best explanatory factors were aspect, slope, site type and soil texture. Models with the highest cumulative probabilities for severe infestation were linked with eastern aspect, moderate steep slope and rich site type fertility (0.72) and eastern aspect, shallow soil and rich site type fertility (0.71). Higher soil C/N ratios with east aspect and rich site type fertility was associated with an increased risk of severe infestation in a third model. The lowest risk was associated with southern and southwestern aspects, fine soil texture, moderate site fertility and gentle slopes.  相似文献   

Hormonal Response of Female Goats to Active Immunization against a Recombinant Human Inhibin α-subunit, and establishment of an Enzyme-linked Immunosorbent Assay for Caprine Follicle-stimulating Hormone     

M Hennies  JK Voglmayr  E Dietrich  M Stollmann  R Moeller  & W Holtz 《Reproduction in domestic animals》2001,36(2):65-71

The effect of selective immunosuppression of endogenous inhibin in goats on FSH, LH, progesterone and estradiol-17β profiles was studied during the breeding and nonbreeding seasons. Eighteen adult female Boer goats were immunized against the recombinant human inhibin α-subunit (hINH-α). With the exception of estradiol, which was determined by radio-immunoassay (RIA), all plasma hormone concentrations were determined by ELISA. The ELISA for FSH presented in this paper was established in the authors' laboratory, based on an existing RIA. Mean basal concentrations of FSH were not affected by immunosuppression of endogenous inhibin, nor was there a difference in the amplitude of the pre-ovulatory FSH surge. Immunization against inhibin appears to eliminate the slight secondary rise of FSH occurring 12–20 h after the major surge associated with ovulation. The LH profiles of the immunized goats were characterized by lower basal concentrations both before and after the pre-ovulatory LH surge which itself was reduced by 50% in immunized does. By contrast, concentrations of circulating estradiol were significantly elevated after inhibin-immunization. Progesterone profiles were not affected. Extending immunization into the anoestrous season by a booster injection of hINH-α, implicating oestrus induction with a progestagen and eCG, produced no discernible differences in FSH and LH profiles in comparison with nonimmunized control goats. The findings suggest that in goats, paracrine factors may play a more significant role in controlling follicular activity than a feedback mechanism acting via the pituitary.  相似文献   

Extraesophageal reflux and reflux aspiration in dogs with respiratory diseases and in healthy dogs     

Sirkku Kouki  Sanna J. Viitanen  Ninna Koho  Henna P. Laurila  Liisa Lilja-Maula  Saila Holopainen  Mikko Neuvonen  Mikko Niemi  Aline Fastrès  Cécile Clercx  Minna M. Rajamäki 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2023,37(1):268-276

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Etiology of respiratory disease in non-vaccinated, non-medicated calves in rearing herds     

Autio T  Pohjanvirta T  Holopainen R  Rikula U  Pentikäinen J  Huovilainen A  Rusanen H  Soveri T  Sihvonen L  Pelkonen S 《Veterinary microbiology》2007,119(2-4):256-265

The aim of this study was to examine the occurrence of bacterial, mycoplasmal and viral pathogens in the lower respiratory tract of calves in all-in all-out calf-rearing units. According to clinical status, non-medicated calves with and without respiratory disease signs were selected of the 40 herds investigated to analyse the micro-organisms present in healthy and diseased calves. Tracheobronchial lavage (TBL) and paired serum samples were analysed for bacteria, mycoplasmas, respiratory syncytial virus (RSV), parainfluenza virus 3 (PIV3), bovine corona virus (BCV) and bovine adenovirus (BAV). Pasteurella multocida was the most common bacterial pathogen. It was isolated from 34% of the TBL samples in 28 herds and was associated with clinical respiratory disease (p < 0.05) when other pathogenic bacteria or mycoplasma were present in the sample. Mannheimia spp. and Histophilus somni were rarely found. Mycoplasma bovis was not detected at all. Ureaplasma diversum was associated with clinical respiratory disease (p < 0.05). TBL samples from healthy or suspect calves were more often negative in bacterial culture than samples from diseased calves (p < 0.05). No viral infections were detected in six herds, while 16-21 herds had RSV, BCV, BAV or PIV3. In the herds that had calves seroconverted to BCV, respiratory shedding of BCV was more frequently observed than faecal shedding. This study showed that the microbial combinations behind BRD were diverse between herds. M. bovis, an emerging pathogen in many countries, was not detected.  相似文献   

Trait perfectionism strengthens the negative effects of moral stressors occurring in veterinary practice          下载免费PDF全文

MF Crane  JK Phillips  E Karin 《Australian veterinary journal》2015,93(10):354-360

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Beak and feather disease virus genotypes in Australian parrots reveal flexible host‐switching          下载免费PDF全文

S Sarker  JK Forwood  SA Ghorashi  A Peters  SR Raidal 《Australian veterinary journal》2015,93(12):471-475

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Blood Metabolite Profiles in Cycling and Non‐cycling Friesian–Sanga Cross‐bred Cows Grazing Natural Pasture During the Post‐partum Period          下载免费PDF全文

FY Obese  C MacCarthy  R Osei‐Amponsah  RA Ayizanga  JK Damptey 《Reproduction in domestic animals》2015,50(2):304-311

An experiment was conducted to investigate the effect of plasma concentrations of the metabolic hormones [Growth hormone (GH), insulin and insulin‐like growth factor –I (IGF‐I)] and nutritional metabolites (Glucose, cholesterol, total protein, albumin, globulin, urea and creatinine) on the resumption of post‐partum ovarian activity in sixteen Friesian–Sanga cows grazing extensively on native grassland. Blood samples were taken from cows from week 1 to 16 post‐partum. Cows were classified as having resumed ovarian activity when a plasma progesterone concentration of ≥ 1.0 ng/ml was recorded for two consecutive weekly samples. Based on the resumption of ovarian activity, cows were classified as early‐cycling, late‐cycling or non‐cycling. The concentrations of the metabolic hormones were measured from week 1 to 10, while those of the nutritional metabolites were measured during week 1, 3, 5, 7 and 9 during the study period. The concentrations of the metabolic hormones, GH and insulin were similar (p > 0.05) in the three ovarian activity groups, likewise the concentrations of the nutritional metabolites, glucose, total protein, globulin, urea and creatinine. Plasma IGF‐I concentration was higher (p < 0.001) in early‐cycling (18.7 ± 0.74 ng/ml) than in late‐cycling (12.4 ± 0.75 ng/ml) and non‐cycling (10.4 ± 0.91 ng/ml) cows. Plasma cholesterol concentrations were significantly lower (p < 0.05) in early‐cycling (1.94 ± 0.15 mmol/l) compared with late‐cycling (2.48 ± 0.12 mmol/l) and non‐cycling (2.61 ± 0.11 mmol/l) cows. For plasma albumin concentrations, the levels recorded for early‐cycling cows were higher (40.7 ± 2.85 g/l) than in late‐cycling (34.4 ± 1.97 g/l) and non‐cycling (33.6 ± 2.66) cows. The results suggest that cows with lower plasma concentrations of IGF‐I and albumin, but higher plasma cholesterol concentrations were at risk of delayed resumption of post‐partum ovarian activity.  相似文献   

Increases in volatile organic compound emissions of Scots pine in response to elevated ozone and warming are modified by herbivory and soil nitrogen availability     

Minna Kivimäenpää  Rajendra P. Ghimire  Sirkka Sutinen  Elina Häikiö  Anne Kasurinen  Toini Holopainen  Jarmo K. Holopainen 《European Journal of Forest Research》2016,135(2):343-360

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Whole‐community facilitation by beaver: ecosystem engineer increases waterbird diversity          下载免费PDF全文

Petri Nummi  Sari Holopainen 《水产资源保护:海洋与淡水生态系统》2014,24(5):623-633