Effect of dopamine on excretory urographic image quality and the prevention of contrast-induced nephropathy in dogs     

Choi J  Lee H  Chang D  Lee K  Eom K  Lee Y  Choi M  Yoon J 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(4):383-388

The effect of low dose dopamine on the excretory urographic image quality and contrast media-induced nephropathy in normal dogs (experiment 1) and the dogs with decreased renal function (experiment 2) were assessed. In experiment 2, decreased renal function was induced by gentamicin overdose. In each experiment, animals were divided into 3 groups. In group 1, only contrast medium (iohexol) was administered. In group 2, contrast medium plus intravenous fluid (0.9% saline) were administered. And in group 3, contrast medium plus intravenous fluid and low dose dopamine were administered. Investigated parameters included intrarenal resistive index (RI), serum BUN and creatinine concentrations, contrast medium elimination time and radiographic image quality. In experiment 1, RI of group I increased at 80 min after contrast medium administration (p<0.05), but RI of group 3 decreased at 48 and 72 hr (p<0.05). Serum BUN concentration of group 1 was higher than that of group 2 and 3 (p<0.05); in radiographic examination, contrast medium elimination time decreased in group 2 and 3, but image quality of group 2 was inferior to that of group 3. In experiment 2, image quality of group 3 only provided adequate visualization of renal structures. The formula of contrast medium plus low dose dopamine was found to provide good nephrogram and pyelogram image quality without supplemental contrast medium, and to protect renal tubules from prolonged exposure to concentrated contrast medium.  相似文献   

First report of bacterial leaf blight of white-flowered calla lily caused by Xanthomonas campestris pv. zantedeschiae in Taiwan     

Y.-A. Lee †  K.-P. Chen  Y.-C. Chang 《Plant pathology》2005,54(2):239-239

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O型口蹄疫病毒VP1嵌合基因的构建及原核表达   总被引：9，自引：0，他引：9  

郑敏  金宁一  鲁会军  韩松  金扩世  李昌 《中国兽医学报》2005,25(6):561-563

首先合成我国O型口蹄疫病毒2个疫苗毒株VP1基因的3个抗原袁位，与另外扩增的流行毒株VP1基因末端273bp片段相连,构建出O型VP1嵌合基因片段（VP1O）。然后，将VPIO基因连接到原核表达载体pET28a上，构建了重组表达质粒pET28a-VP1O，转化大肠杆菌BL21（DE3）进行诱导表达。SDS-PAGE电泳表明。VP1O基因在大肠杆菌中获得表达。Western blotting检测证实表达的VP1O蛋白具有良好的生物学活性。对表达蛋白通过包涵体洗涤的方法进行初步纯化，获得了较高纯度的VP1O蛋白。  相似文献   

Effects of beta2-adrenergic blocker on cytosolic Ca2+ in isolated cardiomyocytes of rats with myocardial infarction     

YANG Hui  WU Wei  FANG Chang  DENG Chun-yu  ZHANG Yan 《园艺学报》2005,21(12):2346-2349

AIM: To investigate if beta2-adrenergic receptors result in more Ca2+ load after myocardial infarction (MI), the effects of beta2-adrenergic blocker on cytosolic Ca2+ (［Ca2+］i) were studied. METHODS: Male Wistar rats underwent a ligation of left coronary artery (n=9) or a sham operation (n=3). Cardiomyocytes were dissociated at 2, 4 and 8 weeks after MI and ［Ca2+］i was measured via fura-2 fluorescence. The response of cardiomyocytes to isoproterenol (1 μmol/L) in the presence or absence of atenolol (1 μmol/L), beta2-adrenergic blocker ICI118,551 (0.1 μmol/L) or propranolol (1 μmol/L) was examined. RESULTS: ICI118,551 suppressed the increase in ［Ca2+］i induced by isoproterenol at 4 and 8 weeks after MI (24.5%±5.7% vs 57.8%±13.2%, P<0.01; 12.2%±7.9% vs 44.6%±11.3%, P<0.01), but had no effects in control and 2 weeks post-MI groups. It decreased ［Ca2+］i in control and the three post-MI groups by 14.3%, 7.9%, 57.6% and 72.6%, respectively. Atenolol had suppressive effects only in control and 2 weeks post-MI groups (P<0.05). Propranolol had suppressive effects in control and all three post-MI groups (P<0.01). CONCLUSION: Beta 2-adrenergic blocker ICI118,551 exerts negative effects on ［Ca2+］i after MI, and the effects dramatically increase with the progression of MI.  相似文献   

ULTRASONOGRAPHIC DIAGNOSIS—PYLOROGASTRIC INTUSSUSCEPTION IN A DOG     

Heechun Lee  Seongchan Yeon  Hyojong Lee  Dongwoo Chang  Kidong Eom  Junghee Yoon  Hojung Choi  Youngwon Lee 《Veterinary radiology & ultrasound》2005,46(4):317-318

A 7-year-old female Maltese presented for evaluation of severe vomiting. A diagnosis of pylorogastric intussusception was made during ultrasonographic examination. The intussusception spontaneously underwent reduction by the following morning.  相似文献   

饲料安全检测及其发展趋势(下)     

常碧影  张丽英 《饲料广角》2005,(4):17-20

3饲料安全检测的依据、方法的性能要求3.1饲料安全检测的依据饲料安全检测与法律、法规相互支持、相互依存。我国1999年颁布实施了《饲料和饲料添加剂管理条例》,2001年结合饲料安全的新形势,修改并重新颁布实施了该《条例》和《兽药管理条例》,后来又出台《饲料药物添加剂使用规范》、农业部193号公告《食品动物禁用的兽药及其他化合物清单》和关于饲料饮水中禁止使用克仑特罗等的发文。这些法规条例规定:药品不得直接加入饲料中使用,必须制成预混剂(即饲料药物添加剂),而预混剂又必须按农业部发布的饲料药物添加剂允许使用品种及标准的规…  相似文献   

蛋白质三级结构预测方法简述     

张漫  常延琦 《中国动物检疫》2005,22(5):36-37

随着各种生物全基因组测序工作的不断深入，越来越多的核酸序列清晰的呈现在世人面前。与此同时，我们明确知道空间结构的蛋白质数目却增长缓慢，而且两者之间增长速度的差异正在进一步扩大。因为蛋白质的生物学功能在很大程度上取决于其空间结构，所以弄清楚蛋白质的结构进而理解其结构与功能的关系具有重要意义。但通过实验方法获得蛋白质结构不仅成本高而且速度慢，  相似文献   

三种生物制剂防治人参立枯病的初步研究     

张连学  朱桂香  常维春  钱树文  柳丽 《植物保护学报》1992,19(2):106-106,116

立枯病(Rhizoctonia solani)系人参苗期主要病害,发病率一般在8%-32%,严重者可达40%。  相似文献   

奶牛发情周期中毛 唾液和乳汁孕酮水平的变化   总被引：1，自引：0，他引：1  

张家骅  黄群山 《黑龙江畜牧兽医》1992,(11):5-7

本实验采用放射免疫分析法(RIA)测定了11头奶牛一个发情周期里毛、唾液、乳汁中孕酮(P_4)含量。经产牛(n=8)的毛和唾液中P_4水平变化与乳汁中P_4水平变化一致。青年牛(n=3)的毛和睡液中的P_4水平变化一致,并分别与经产牛毛和唾液中P_4水平变化一致。7头配种后的经产牛和5头配种后的青年牛分别取20和23天的样品进行妊娠诊断,与配种后60天的直检结果相对照,依据经产牛毛、乳P_4水平进行妊娠诊断的阳性准确率分别是85.7％(6/7)和100％(7/7),依据青年牛毛P_4水平进行妊娠诊断的阳性准确率为100％(5/5)。  相似文献   

China’s Crop­Land Use, 1957     

Sen-Dou Chang 《Asia Pacific viewpoint》1971,12(1):75-87

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