Prevalence of house dust mites in the homes of atopic dogs in Finland          下载免费PDF全文

Kirsti J. M. Schildt  Riitta T. K. Seppänen  Anna K. Hielm‐Björkman  Leena E. Saijonmaa‐Koulumies  Svetlana Belova 《Veterinary dermatology》2017,28(2):225-e54

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Numerical Modelling of Waste Stabilization Ponds: Where Do We Stand?     

Leena Sah  Diederik P. L. Rousseau  Christine M. Hooijmans 《Water, air, and soil pollution》2012,223(6):3155-3171

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Enhanced production of dihaploid lines via anther culture of tetraploid potato (Solanum tuberosum L. ssp.tuberosum) clones     

Veli-Matti Rokka  Leena Pietilä  Eija Pehu 《American Journal of Potato Research》1996,73(1):1-12

A total of 1000 anther-derived plants was regenerated from tetraploid potato (Solanum tuberosum L.) genotypes. Capacity to undergo androgenesis was analysed in 41 potato cultivars and 7 clones grown either in the greenhouse or in the field. Of the 48 genotypes, 33 produced embryos and 23 regenerated shoots from embryos. Anther-derived plantlet production was determined in genotypes 86110, Agria, Calgary, Escort, Helios, Idole, JO 0982 JO 1432, Kainuun Musta, Kardal, KE48, Matilda, Nicola, Petra, Pito, Rustica, Stirling, Torridon, Ute, Van Gogh, Vebeca, Vento and White Lady. The highest number of shoots (24 shoots/100 anthers) was obtained from cv. Calgary, when anthers were isolated from field-grown donor plants. Incubating anthers at 28 C, rather than at 20 C or 24 C, enhanced embryo production in four genotypes tested. However, shoot production was improved only in cv. Pito cultured at 28 C. When anthers of cv. Petra were cultured at 28 C for four weeks, followed by reduction of culture temperature to 24 C, a high rate of shoot production was recorded (14 shoots/100 anthers). The ratio between dihaploids and tetraploids varied among the anther-derived plants of the different genotypes. The number of dihaploids was highest in potato clone JO1432 (100%) and in cv. Calgary (93%) and lowest in cvs. Pito (21%) and Torridon (6%).  相似文献   

NPAS2 and PER2 are linked to risk factors of the metabolic syndrome     

Ani Englund  Leena Kovanen  Sirkku T Saarikoski  Jari Haukka  Antti Reunanen  Arpo Aromaa  Jouko Lönnqvist  Timo Partonen 《Journal of circadian rhythms》2009,7(1):5-9

Background  

Mammalian circadian clocks control multiple physiological events. The principal circadian clock generates seasonal variations in behavior as well. Seasonality elevates the risk for metabolic syndrome, and evidence suggests that disruption of the clockwork can lead to alterations in metabolism. Our aim was to analyze whether circadian clock polymorphisms contribute to seasonal variations in behavior and to the metabolic syndrome.  相似文献   

Production and characterization of “second generation” somatic hybrids derived from protoplast fusion between interspecific somatohaploid and dihaploidSolanum tuberosum L.     

Veli-Matti Rokka  Jari P. T. Valkonen  Airi Tauriainen  Leena Pietilä  Renata Lebecka  Ewa Zimnoch-Guzowska  Eija Pehu 《American Journal of Potato Research》2000,77(3):149-159

Protoplasts were fused to produce somatic hybrids between a triploid (2n=3x=32-34) interspecific somatohaploid betweenSolanum brevidens Phil. andS. tuberosum L., and a dihaploid (2n=2x=24) anther-derived line ofS. tuberosum cv. Van Gogh. A total of 265 plants were regenerated from protoplast fusion derived calli and their hybridity was verified using fusion partner specific RAPD markers. These “second generation” somatic hybrids were aneuploid pentaploids (2n=5x=51-65) with a 2C DNA content ranging from 3.36 to 4.43 pg, which corresponded to the sum of the 2C values of each of the fusion partners (somatohaploid: 2.22 pg; and the dihaploid line of cv. Van Gogh: 1.87 pg). Most of the “second generation” somatic hybrids were vigorous, but variable in morphology. They were extremely resistant to PLRV and they had tolerance to PVY infection derived from the somatohaploid fusion partner. Even though most of the “second generation” hybrids tuberized, the tuber morphology was variable and most were poorly shaped. InErwinia soft rot resistance tests, the tubers showed higher level of resistance than the tetraploidS. tuberosum cultivars, the dihaploidS. tuberosum fusion partners and the hexaploid somatic hybrids betweenS. brevidens andS. tuberosum. The “second generation” somatic hybrids were all male sterile and failed to produce berries or seeds.  相似文献   

The ecosystem services concept: a new Esperanto to facilitate participatory planning processes?     

Spyra  Marcin  Kleemann  Janina  Cetin  Nuket Ipek  Vázquez Navarrete  Cesar Jesús  Albert  Christian  Palacios-Agundez  Igone  Ametzaga-Arregi  Ibone  La Rosa  Daniele  Rozas-Vásquez  Daniel  Adem Esmail  Blal  Picchi  Paolo  Geneletti  Davide  König  Hannes J.  Koo  HongMi  Kopperoinen  Leena  Fürst  Christine 《Landscape Ecology》2019,34(7):1715-1735

Landscape Ecology - Several case studies investigated the role of ecosystem services in participatory planning processes. However, no systematic study exists that cuts across a large number of...  相似文献   

Effects of timing of soil frost thawing on Scots pine     

Repo T  Kalliokoski T  Domisch T  Lehto T  Mannerkoski H  Sutinen S  Finér L 《Tree physiology》2005,25(8):1053-1062

Effects of the timing of soil thawing in the spring on Scots pine (Pinus sylvestris L.) were studied under controlled laboratory conditions. Sixteen 6-year-old saplings were lifted from the field, replanted in containers and placed in four treatments in controlled environment (CE) chambers with four replicate saplings per chamber. The saplings were held in the CE chambers during one simulated winter and one simulated growing season. The soil was frozen to -2 degrees C during a second simulated winter in the CE chambers, and the soil thawing treatments began at the end of the second simulated winter. Soil thawing began at various times before (no delay in thawing) and after (delay in thawing) chamber air conditions were changed from simulated winter to simulated summer. Delayed soil thawing subjected saplings to stress, with the severity of stress depending on the length of the delay in thawing. If there was no delay or only a short delay in soil thawing, stress was minor and reversible. A 2-week delay in soil thawing led to death of the saplings. Stress was apparent as decreases in the variable to maximal chlorophyll fluorescence ratio (Fv/Fm), chlorophyll a/b ratio and needle water potential. In needles of stressed saplings, apoplastic electrical resistance first decreased and then increased and there were anomalies in the electrical impedance spectra of the stems. Stress from the soil thawing treatments affected both root and shoot growth.  相似文献   

Purposes and challenges of public participation in regional and local forestry in Finland     

Leena A. Leskinen   《Forest Policy and Economics》2004,6(6):315

Public participation in regional and local forest planning in Finland was researched. The questions were: (1) What is the purpose of public participation? (2) What are the challenges of public participation? The results are based on the analysis of discourse communities in a case study concerning a Regional Forest Programme and forest management planning. It was shown that the purpose of participation was to promote communication at the regional level and to gather information at the local level. The general conclusion is, that the regional forest programmes include practices to offer possibilities for communication among participants. This does not mean, that all the programme processes have reached consensus automatically. The case studied here reached compromise. The case study points out problems faced, if public participation would become common practice at the local level on non-industrial private forests lands. In forest management planning it would result in an excessively demanding mediator/expert role for the planner. The need to consider all relevant issues, e.g. biodiversity management, would also become apparent. The study supports the idea that public participation as communication has better possibilities to promote sustainable forest management than public participation as information gathering.  相似文献   

Antifungal activity of the stem bark of Ailanthus excelsa     

Joshi BC  Pandey A  Chaurasia L  Pal M  Sharma RP  Khare A 《Fitoterapia》2003,74(7-8):689-691

The methanol extract of stem barks of Alianthus excelsa was partitioned with chloroform. The chloroform extract showed fungistatic and fungicidal activity against Aspergillus niger, A. fumigatus, Penicillium frequentence, P. notatum and Botrytis cinerea.  相似文献   

Use of ITS nuclear sequences from Phelipanche aegyptiaca as a direct tool to detect single seeds of broomrape species in the soil     

Radi Aly  Hanan Eizenberg  Marina Kocherman  Jacklin Abu-Nassar  Leena Taha  Ibrahim Saadi 《European journal of plant pathology / European Foundation for Plant Pathology》2012,133(3):523-526

Broomrape (Phelipanche and Orobanche spp.) are obligate holoparasites that attack roots of almost all economically-important crops in semiarid regions of the world. Broomrape seeds are extremely small (dust-like seeds), averaging 200 to 300?μm in size and because of the miniscule seed size it is difficult to detect and confirm via conventional methods. In this study our aim was to develop a PCR-based assay specific for broomrape soil-borne seeds and sensitive enough to detect a single or few broomrape seeds in a soil sample. For this purpose, we used complementary polymerase chain reaction (PCR) primers based upon unique sequences in the internal transcribed spacer (ITS) regions of the nuclear ribosomal DNA of Phelipanche aegyptiaca. Genomic DNA was extracted from soil samples artificially infested with broomrape seeds or tissue of Phelipanche aegyptiaca Pers., Orobanche cumana Wallr. and Phelipanche crenata Forsk. and subjected to PCR analysis. Using ITS-350 primers, a specific PCR product (350?bp) was amplified and detected in all samples containing broomrape species, but was not detected in soil sample free of broomrape seeds or tissues. Additionally, the PCR-based assay was sensitive enough to detect even a single broomrape seed in the soil. As expected the universal internal control primers amplified a PCR product (555?bp) of genomic DNA extracted from soil samples with or without broomrape tissues or seeds. This diagnostic method is simple, reliable and rapid and could help for assessment of broomrape seed contamination in a crop field.  相似文献