Clinical, behavioral, and pulmonary changes in calves following inoculation with Mycoplasma bovis     

White BJ  Anderson DE  Renter DG  Larson RL  Mosier DA  Kelly LL  Theurer ME  Robért BD  Walz ML 《American journal of veterinary research》2012,73(4):490-497

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Comparison of radiographic osteoarthritis scores in dogs less than 24 months or greater than 24 months following tibial plateau leveling osteotomy     

Andrea M. DeLuke  Dave A. Allen  Eric R. Wilson  Jayce A. Lineberger  Terry W. Lehenbauer  Michelle Fabiani  Brian A. Poteet 《The Canadian veterinary journal. La revue veterinaire canadienne》2012,53(10):1095-1099

Radiographic osteoarthritis scores were determined in 60 dogs up to 3 years following tibial plateau leveling osteotomy. Radiographs taken immediately following surgery and at long-term follow-up 1 to 3 years later were evaluated by 2 certified radiologists using a modified 32-point osteoarthritis scale. Changes in osteoarthritis scores were evaluated by paired t-tests and regression analysis. Sub-groups of dogs were formed to evaluate if osteoarthritis scores changed differently for follow-up periods of < 24 months compared with those > 24 months. There was a significant increase in osteoarthritis score from post-operative to follow-up evaluations for all dogs studied. Regression analysis of the relationship of months after surgery to change in osteoarthritis score was not significant. These results indicate that osteoarthritis did progress following tibial plateau leveling osteotomy, but not in a linear fashion over time.  相似文献   

Molecular typing of Mycoplasma agalactiae: tracing European-wide genetic diversity and an endemic clonal population     

Nouvel LX  Marenda MS  Glew MD  Sagné E  Giammarinaro P  Tardy F  Poumarat F  Rosengarten R  Citti C 《Comparative immunology, microbiology and infectious diseases》2012,35(5):487-496

Mycoplasma agalactiae causes chronic infections in small ruminants and remains endemic in many regions of the world, despite intensive and costly eradication programs. In this study, the innate genomic plasticity of M. agalactiae was exploited to design and assess a combination of molecular epidemiological tools to trace the pathogen in different geographic locations and to understand its emergence or re-emergence after eradication campaigns. For this purpose, two collections of M. agalactiae isolates, representing European outbreaks or localized endemic disease in a single region of France, were subjected to RFLP (Restriction Fragment Length Polymorphism) analyses using two sets of DNA probes (distributed across the genome and specific for the vpma gene locus), and a previously described VNTR (Variable Number Tandem Repeats) analysis. A combination of four genome-specific DNA probes and two VNTRs gave the highest discriminative power. Molecular typing revealed that, while isolates from diverse geographical origins fell into clearly different groups, the endemic disease repeatedly observed in the Western Pyrenees region over the past 30 years has been caused by a unique subtype of M. agalactiae. This indicates that the re-emergence of the pathogen after seemingly successful eradication programs is not due to the importation of exotic strains, but to the persistence of local reservoirs of infection.  相似文献   

Hematologic and IgG responses of heifers experimentally infected with the agent of epizootic bovine abortion     

Coker MR  Rauw WM  Nieto NC  Thain D  Teglas MB 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2012,41(3):344-352

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Exotic pet management for the technician.     

Michelle S Schulte  Agnes E Rupley 《Veterinary Clinics of North America: Exotic Animal Practice》2004,7(2):xi-xii

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Boron and zinc deficiencies and toxicities and their interactions with other nutrients in soybean roots,leaves, and seeds     

Michelle L. Pawlowski  Julian Helfenstein  Emmanuel Frossard 《Journal of plant nutrition》2019,42(6):634-649

In many parts of the world, soils deficient and/or toxic in micronutrients reduce potential soybean (Glycine max) yields. The objective of our study was to grow plants in low to high concentrations of boron (B) and zinc (Zn) to determine how B and Zn deficiencies and toxicities affect soybean growth and interact with other essential nutrients in roots, leaves, and seeds. We found that B significantly affected levels of all essential nutrients except manganese and iron, while Zn significantly affected all essential nutrients in at least one plant tissue tested. Some of the physiological responses and nutrient interactions were cultivar-dependent. This study showed how deficiencies and toxicities of B and Zn affect plant growth and how B and Zn fertility interacts with many of the other essential nutrients.  相似文献   

School food in Mexican children     

Lozada M  Sánchez-Castillo CP  Cabrera GA  Mata II  Pichardo-Ontiveros E  Villa AR  James WP 《Public health nutrition》2008,11(9):924-933

OBJECTIVE: To establish the school eating habits of Mexican children, who are prone to obesity and later to high rates of adult chronic diseases. DESIGN: Questionnaires for students and parents with staff questionnaires and interviews. SETTING: Randomly sampled schools in a socio-economically representative district of Mexico City. SUBJECTS: Subjects were 1504 adolescents aged 10-19 years attending schools in Mexico City, 27 teachers and seven headmasters, sampled from both public and private schools and from the full range of socio-economic groups. RESULTS: Foods brought from home were of a higher nutritional quality than those purchased at school, where purchases were dominated by crisps, soft drinks and other items with high energy density. Girls were more inclined to purchase inappropriately; those from poorer homes purchased less. Private-school students irrespective of socio-economic grade brought more food from home and purchased more expensive food at school. School policies allowed food and drink vendors to market any products within the schools, which benefited financially from these activities. CONCLUSIONS: Current school food policies are conducive to amplifying the current epidemic of obesity and related adult chronic diseases, and need to change.  相似文献   

Analysis of chlorothalonil and three degradates in sediment and soil   总被引：2，自引：0，他引：2  

Hladik ML  Kuivila KM 《Journal of agricultural and food chemistry》2008,56(7):2310-2314

A method has been developed for the simultaneous extraction of chlorothalonil and three of its degradates (4-hydroxy-2,5,6-trichloroisophthalonitrile, 1-carbamoyl-3-cyano-4-hydroxy-2,5,6-trichlorobenzene, and 1,3-dicarbamoyl-2,4,5,6-tetrachlorobenzene) from soils and sediments; the compounds were extracted using sonication with acetone and isolation of the parent compound and matrix interferences from the degradates by solid phase extraction (SPE). The chlorothalonil fraction underwent further coextracted matrix interference removal with Florisil. The degradates were derivatized with N, O-bis(trimethylsilyl) trifluoroacetamide (BSTFA) and chlorotrimethylsilane (TMCS). All compounds were analyzed by gas chromatography-mass spectrometry (GC-MS). Recoveries on a spiked (20 and 200 microg kg (-1)) sediment ranged from 80% to 91% with calculated limits of detection of 1-5 microg kg (-1) dry weight sediment. An additional 20 sediment samples were collected in watersheds from the Southeastern United States where chlorothalonil is used widely on peanuts and other crops. None of the target compounds were detected. Laboratory fortified recoveries of chlorothalonil and its degradates in these environmental sediment samples ranged from 75% to 89%.  相似文献   

Microbiological and chemical characterization of a typical Italian cheese: Robiola di Roccaverano     

Bonetta S  Coïsson JD  Barile D  Bonetta S  Travaglia F  Piana G  Carraro E  Arlorio M 《Journal of agricultural and food chemistry》2008,56(16):7223-7230

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Dynamics in debris-flow activity on a forested cone — A case study using different dendroecological approaches     

Michelle Bollschweiler  Markus StoffelDominique M. Schneuwly 《CATENA》2008

Dendrogeomorphological analyses of trees affected by debris flows have regularly been used to date past events. However, this method has always been limited to forested cones where trees registered the impact of previous events. The minimum age dating of trees growing in the debris deposits can, in contrast, provide information on the latest possible moment of past activity. In this paper, we report on results obtained from a combination of these two approaches on a forested cone in the Valais Alps (Switzerland). A detailed geomorphic map in a scale of 1:1000 served as a basis for the sampling strategy. Disturbed Larix decidua Mill. and Picea abies (L.) Karst. trees growing in the deposits allowed reconstruction of 49 events between AD 1782 and 2005 as well as the determination of the spatial extent of events. In the debris-flow channels where survivor trees are missing, we selected the oldest post-event trees and assessed their age by counting their growth rings. Missing rings due to lack of center as well as to sampling height were added so as to determine real tree age. The combination of the dendrogeomorphological event reconstruction with the assessment of germination dates of successor trees allowed realistic approximation of the minimum time elapsed since the last debris-flow activity in 23 of the 29 channels present on the current-day cone surface. In general, channels in the northern part of the cone and those close to the currently active channel generally show signs of (sub-) recent activity with one last overbank sedimentation event in the 1980s, whereas signs of debris-flow activity are absent from the channels in the outermost part since the late 19th century. As a consequence of the deeply incised channel and the stabilization measures undertaken along the banks, signs of debris flows are missing in the tree-ring record for the past two decades.  相似文献