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81.
The variation in egg size within and between egg clutches of O. mossambicus, and the subsequent influence of mean egg size on growth, feeding incidence and survival of unfed fry were investigated.The individual egg sizes within 90% of the egg clutches examined were normally distributed and the coefficient of variation of egg size within clutches ranged from 7.4% to 15.4%. Fry emerging from the small, medium and large egg size groups reached their maximum length and weight at 9, 9 and 12 days after hatching, respectively. Maximum attainable fry length and weight were significantly correlated with egg size (r = 0.885 and 0.947, respectively, with P < 0.01).Egg size had little influence on the onset of feeding. Fry were capable of exogenous feeding within 6 to 7 days of hatching. Fry originating from small and medium egg size classes reached the point-of-no-return at 15–16 days after hatching compared to 21 days for fry from the large egg size class. The mean survival time (ST50) of fry on their yolk reserves was significantly correlated (r = 0.923; P < 0.01) with their original mean egg weight and ranged from 9.5 days for fry hatching from 0.91-mg eggs to 21 days for fry from 1.93-mg eggs.  相似文献   
82.
Aeromonas salmonicida, a fish pathogen, is the causative agent of furunculosis. It was already shown that growing this bacterium in stressful conditions such as temperature above 22°C might lead to virulence attenuation. Unfortunately, many veterinary microbiology services and reference centers still routinely cultivate A. salmonicida at 25°C. Here we tested the presence of virulence factors by growth on specific medium as well as the integrity of the pAsa5 plasmid, which bears an important virulence factor, the type III secretion system (TTSS), by PCR analysis in twenty strains, most of which were grown at 25°C in their laboratory of origin. The analysis revealed that strains, which encountered the more stressful growth conditions displayed the most frequent absence of A-layer protein and secreted proteolytic activity. Moreover, many strains had lost parts of the pAsa5 plasmid in which the TTSS region was almost always affected. To confirm the effect of stressful growth conditions on the plasmid, three strains with an intact pAsa5 were cultured at 25°C for two weeks. A low but significant fraction of the tested colonies displayed pAsa5 rearrangements. The rearrangement always affected the TTSS region and led to a loss of virulence in the Dictyostelium discoideum co-culture assay. These results demonstrate that the instability of pAsa5 did not lead to its complete loss as previously proposed but to a more complex rearrangement phenomenon and emphasizes the necessity to grow A. salmonicida in appropriate conditions to preserve the complete virulence of the bacterium.  相似文献   
83.
The Australian ruminant livestock industries are faced with the need to control parasitic infectious diseases that can seriously impact the health of animals. However, increasing levels of resistance to insecticides, anthelmintics and acaricides are substantially reducing the ability to control some of these parasites. Here we review the current situation with regard to chemical resistances in parasites across the various sectors of the Australian ruminant livestock industries and assess the level of threat that these resistances pose to the sustainability of these sectors in the short to long terms. We also look at the extent to which testing for resistance occurs across the various industry sectors, and hence how well-informed these sectors are of the extent of chemical resistance. We examine on-farm management practices, breeding of parasite-resistant animals, and non-chemical therapeutics that may act as short to long term means to reduce the current reliance on chemicals for parasite control. Finally, we look at the balance between the prevalence and magnitude of current resistances and the availability and adoption rates of management, breeding and therapeutic alternatives in order to assess the parasite control outlook for the various industry sectors.  相似文献   
84.
85.
Rajarajan  K.  Uthappa  A. R.  Handa  A. K.  Chavan  S. B.  Vishnu  R.  Shrivastava  A.  Handa  A.  Rana  M.  Sahu  S.  Kumar  N.  Singh  A. K.  Ahmed  S.  Rana  M. 《Genetic Resources and Crop Evolution》2022,69(1):71-83
Genetic Resources and Crop Evolution - For the estimation of genetic diversity and population structure, 23 accessions of Leucaena leucocephala were characterized using morphological and molecular...  相似文献   
86.
The nutritive value of some commercial wheat varieties grown in Pakistan was measured chemically, including amino acid analysis, and biologically in N-balance experiments with growing rats. The protein content ranged from 13.2% in Punjab-83 to 16% in Barani-83. Lysine per 100g protein varied between 2.46 and 2.75%. The available carbohydrate ranged from 67.3 to 74.7%. Iron content was highest in Sonalika while the concentration of zinc and manganese was highest in Sarhad-82. The protein digestibility (TD), biological value (BV) and net protein utilisation (NPU) varied between 92–95%, 56–68% and 53–65% respectively. Level of wheat protein was negatively correlated with available carbohydrate (r=–0.93), lysine per unit protein (r=–0.67) and BV (r=–0.76). The lysine content (g/16gN) of commercial wheat varieties showed a positive correlation (r=+0.95) with the BV. The protein quality was lowered in varieties having higher content of protein.  相似文献   
87.
A pot experiment was undertaken under net house conditions, with three rhizobacterial strains AW1 (Bacillus sp.), AW5 (Providencia sp.) and AW7 (Brevundimonas sp.), applied along with 2/3 recommended dose of nitrogen (N) and full dose of phosphorus (P) and potassium (K) fertilizers (N90P60K60). An enhancement of 14–34% in plant biometric parameters and 28–60% in micronutrient content was recorded in treatments receiving the combination of AW1?+?AW5 strains, as compared to full dose of fertilizer application. The treatment involving inoculation with AW5?+?AW7 recorded highest values of % P and N, with a two-fold enhancement in phosphorus and 66.7% increase in N content, over full dose application of P and K fertilizers. A significant correlation was recorded between plant biomass, panicle weight, grain weight, N, P and iron (Fe) with acetylene reduction activity, indicating the significance of N fixation in overall crop productivity. Our study illustrates the multiple benefits of plant growth promoting rhizobacteria (PGPR) inoculation in integrated nutrient management and biofortification strategies for wheat crop.  相似文献   
88.
Abstract

The present study was aimed to anticipate how forest composition, regeneration, biomass production, and carbon storage vary in the ridge top forests of the high mountains of Garhwal Himalaya. For this purpose five major forest types—(a) Pinus wallichiana, (b) Quercus semecarpifolia, (c) Cedrus deodara, (d) Abies spectabilis, and (e) Betula utilis mixed forests—were selected on different ridge tops in the Bhagirathi Catchment Area of the Uttarkashi District of Garhwal Himalaya. The highest species richness (10 species) and stand density (804 ± 184.5 stems ha?1) were recorded in Abies spectabilis forests, whereas lowest species richness (4 species) and species density (428 ± 144.7 stems ha?1) were found in Quercus semecarpifolia forests. The total basal cover (TBC) values were maximum (91.1 ± 24.4 m2 ha?1) in Cedrus deodara forests and minimum (26.5 ± 11.7 m2 ha?1) in Pinus wallichiana forests. The highest total biomass density (TBD) (464.2 ± 152.5 Mg ha?1) and total carbon density (TCD; 208.9 ± 68.6 Mg C ha?1) values were recorded for Cedrus deodara forests; however, lowest TBD (283.4 ± 74.8 Mg ha?1) and TCD (127.5 ± 33.7 Mg C ha?1) values for Quercus semecarpifolia forests. Our study suggests that Abies spectabilis-dominated forests should be encouraged for biodiversity enrichment and reducing carbon emissions on ridge top forests of high mountains.  相似文献   
89.
AIM: To investigate the nature of a neurological disease in Wiltshire sheep.

METHODS: Three affected lambs were examined, humanely killed and necropsied. Selected neurological tissues were examined by light and electron microscopy.

RESULTS: Primary neurological lesions were confined to the cerebellum and were characterised by loss of Purkinje cells and the presence of large hypertrophied dendrites of surviving Purkinje cells. These contained stacks of smooth endoplasmic reticulum. There was hyperplasia and cell swelling of Bergmann glia. Mild Wallerian-type degeneration affected white matter in the cerebellum and spinal cord.

CONCLUSION: The cerebellar lesions were of a degenerative and reactive rather than hypoplastic nature. These, and the history, suggest a genetic cause with putative inheritance as an autosomal recessive trait. Accordingly, the disorder is described as a cerebellar abiotrophy.  相似文献   
90.
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