An Investigation on Biodiversity Perceptions of Students Enrolled in Ecotourism at De La Salle-College of Saint Benilde     

Benigno Glenn  Reyes Ricaforte 《农业科学与技术》2012,(11):1245-1252

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Depletion study and estimation of the withdrawal period for oxytetracycline in tilapia cultured in Brazil     

Paschoal JA  Bicudo ÁJ  Cyrino JE  Reyes FG  Rath S 《Journal of veterinary pharmacology and therapeutics》2012,35(1):90-96

Defining pharmacokinetic parameters and depletion intervals for antimicrobials used in fish represents important guidelines for future regulation by Brazilian agencies of the use of these substances in fish farming. This article presents a depletion study for oxytetracycline (OTC) in tilapias (Orechromis niloticus) farmed under tropical conditions during the winter season. High performance liquid chromatography, with fluorescence detection for the quantitation of OTC in tilapia fillets and medicated feed, was developed and validated. The depletion study with fish was carried out under monitored environmental conditions. OTC was administered in the feed for five consecutive days at daily dosages of 80 mg/kg body weight. Groups of ten fish were slaughtered at 1, 2, 3, 4, 5, 8, 10, 15, 20, and 25 days after medication. After the 8th day posttreatment, OTC concentrations in the tilapia fillets were below the limit of quantitation (13 ng/g) of the method. Linear regression of the mathematical model of data analysis presented a coefficient of 0.9962. The elimination half-life for OTC in tilapia fillet and the withdrawal period were 1.65 and 6 days, respectively, considering a percentile of 99% with 95% of confidence and a maximum residue limit of 100 ng/g. Even though the study was carried out in the winter under practical conditions where water temperature varied, the results obtained are similar to others from studies conducted under controlled temperature.  相似文献   

Origin of adenohypophysial lobes and cells from Rathke's pouch in Swiss albino mice. Proliferation and expression of Pitx 2 and Calbindin D28K in corticotropic and somatotropic cell differentiation     

Reyes R  Martínez S  González M  Tramu G  Bello AR 《Anatomia, histologia, embryologia》2008,37(4):263-271

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Early Pregnancy Diagnosis by Serum Progesterone and Ultrasound in Sheep Carrying Somatic Cell Nuclear Transfer-Derived Pregnancies     

B Alexander  G Coppola  GF Mastromonaco  E St. John  ER Reyes  DH Betts  WA King 《Reproduction in domestic animals》2008,43(2):207-211

Early pregnancy diagnosis and monitoring play an important role following embryo transfer in sheep. The aims of the current study were to investigate (i) the pattern of serum progesterone profiles in sheep carrying somatic cell nuclear transfer (SCNT)‐derived (clone) pregnancies, and (ii) the frequency of pregnancy loss during development following SCNT embryo transfer. Sheep SCNT embryos were made using standard nuclear transfer techniques. Day 7 embryos were surgically transferred to oestrus‐synchronized recipients (n = 27). As a control, normal fertile ewes (n = 12) were bred by natural breeding. Serum was collected from all the ewes on the day of estrus (day 0 sample), 7 days post‐estrus (day 7 sample) and 19 days post‐estrus (day 19 sample) and every 10 days thereafter until lambing or pregnancy loss occurred. Serum progesterone (P4) was assessed using enzyme immunoassay. Pregnancy was confirmed by ultrasound scanning on day 35 of pregnancy followed by subsequent scanning every 10 days. In control ewes, pregnancy rate on day 35 was 83.3% (10/12), whereas in the ewes that received SCNT embryos, it was 22.2% (6/27; p < 0.05). The day 45 pregnancy rate in the control ewes was 83.3%, whereas in the SCNT embryo recipients it was 11.0% (p < 0.05). Hormone analysis revealed that SCNT embryo recipients exhibited a significantly lower P4 profiles at different time points in pregnancy compared to controls (p < 0.05). This study highlights the use of serum progesterone in combination with ultrasound for the investigation of embryo loss and crucial times during development of normal and SCNT embryos in sheep. Further, the serum P4 levels directly reflect the degree of placental development in these two groups.  相似文献   

Physiological considerations for efficient mycelial colonization of Philippine strains ofVolvariella volvacea     

Renato G. Reyes  Fumio Eguchi  Tomoaki Iijima  Miyato Higaki 《Journal of Wood Science》1998,44(5):408-413

The nutritional and physical requirements for the efficient mycelial colonization ofVolvariella volvacea (Bull. ex. Fr.). Singer were elucidated with the percentage mycelial colonization and density as references. This investigation was limited to the evaluation of two commercial strains (designated Vvc1 and Vvc2) and two wild strains (designated EAAC-0001 and EAAC-0002) ofV. volvacea from the Philippines with the aim of providing baseline data on their physiological requirements. The four strains ofV. volvacea had varying preferences for carbon. Vve1 preferred polysaccharides (starch and cellulose), whereas Vvc2 grew luxuriantly at a relatively rapid rate in sugar alcohol (sorbitol). The two wild strains preferred starch as a carbon source. In terms of nitrogen utilization, soytone, peptone, and glycine supported efficient mycelial colonization of the four strains. The vitamin utilization test revealed that ascorbic acid, calcium pantothenate, and biotin are good sources. The mycelial growth performances of the strains were also evaluated on six dehydrated mycological media. Efficient colonization of Vvc1, Vvc2, and EAAC-0002 with dense mycelial growth was noted in mycological agar. EAAC-0001, on the other hand, grew more efficiently in malt extract agar. The Philippine strains ofV. volvacea grew luxuriantly when incubated at 35°C and pH 8.0 under dark and sealed conditions. Moreover, the relatively higher moisture content (70%) of the oolong tea leaf formulation favorably stimulated efficient mycelial colonization. Under optimum physiological conditions, Vvc1, Vvc2, and EAAC-0002 were fast-growing strains, whereas EAAC-0001 was a moderately growing type.  相似文献   

Effects of dietary supplementation with probiotic live yeast Debaryomyces hansenii on the immune and antioxidant systems of leopard grouper Mycteroperca rosacea infected with Aeromonas hydrophila     

Martha Reyes‐Becerril  Dariel Tovar‐Ramírez  Felipe Ascencio‐Valle  Roberto Civera‐Cerecedo  Vicente Gracia‐López  Valérie Barbosa‐Solomieu  María Ángeles Esteban 《Aquaculture Research》2011,42(11):1676-1686

Two trials were conducted to determine the efficacy of fish fed live yeast Debaryomyces hansenii strain CBS 8339 on immune and antioxidant systems in leopard grouper Mycteroperca rosacea infected with Aeromonas hydrophila. Juveniles (12±0.5 g) were fed with a control diet or a D. hansenii‐supplemented diet (10⁶ colony‐forming units per gram) for 5 weeks. The live weight of fish was registered on a weekly basis. After 4 weeks, fish from each treatment were immunocompromised with pathogenic A. hydrophila and further fed for 1 week in order to evaluate the effect on immunological and antioxidant parameters. Generally, the results showed enhanced growth performance in fish fed the diet containing yeast compared with the control. Addition of live yeast had no significant effect on the immunological parameters after 4 weeks of feeding. However, post infection with A. hydrophila fish fed the yeast‐supplemented diet resulted in a significant increase in the levels of plasmatic immunoglobulin M. Superoxide dismutase and catalase (CAT) activities were significantly higher in the yeast group. In this fish, CAT and heat shock protein 70 genes were up‐regulated before and after infection of A. hydrophila. The present study is the first one reporting that yeast (D. hansenii) can enhance immunity and resistance against A. hydrophila.  相似文献   

Evaluation of Agave lechuguilla by‐product crude extract as a feed additive for juvenile shrimp Litopenaeus vannamei     

Alberto Pea‐Rodríguez  Zo Pelletier‐Morreeuw  Jazmín García‐Lujn  María del Carmen Rodríguez‐Jaramillo  Laura Guzmn‐Villanueva  Cristina Escobedo‐Fregoso  Dariel Tovar‐Ramírez  Ana Gisela Reyes 《Aquaculture Research》2020,51(4):1336-1345

Agave lechuguilla is a succulent plant species, mainly distributed in the northeast of Mexico and south of the United States of America. The main use for this plant is the fibre´s extraction (known as Tampico fibres), resulting in 15% of fibres and 85% of a by‐product waste named guishe. The lechuguilla collectors, normally incinerate the guishe, thus causing environmental contamination. Interestingly, recent studies showed that guishe contains molecules with nutritional properties, such as saponins, flavonoids and sugars. Therefore, in this work, we evaluated the effect of the crude extract of guishe as a feed additive in whiteleg shrimp diets. According to that, MS‐HPLC analysis of the extract showed the presence of saponins such as diosgenin, smilagenin, hecogenin, manogenin, tigogenin hexose, yucagenin, chlorogenin, diosgenin diglucoside and the flavonol, quercetin. After chemical analysis, the crude extract was included into an experimental diet in four levels; 0% (L0%), 0.1% (L0.1%), 0.3% (L0.3%) and 0.6% (L0.6%). Dietary incorporation of the extract was evaluated by zootechnical performance, haemolymph biochemistry, histomorphology and digestive enzyme activity of shrimps. After 5‐week feeding, the L0.3% diet showed significantly higher growth and better feed utilization among treatments. A significant increase in tubule epithelium height and tubule coverage area from hepatopancreas in shrimp under L0.3% diet compared with the control diet suggest an improvement of the health and nutritional status of the shrimp. Inclusion of L0.3% and L0.6% of the crude extract resulted in a reduction in amylase activity, without effect in glucose levels in the haemolymph. Thus, we suggest that lechuguilla guishe crude extract contains nutritional molecules that may be used as a feed additive to promote shrimp productivity.  相似文献   

Influence of latitude on the light availability for intercrops in an agroforestry alley-cropping system     

Christian Dupraz  Céline Blitz-Frayret  Isabelle Lecomte  Quentin Molto  Francesco Reyes  Marie Gosme 《Agroforestry Systems》2018,92(4):1019-1033

Light competition by trees is often regarded as a major limiting factor for crops in alley-cropping agroforestry. Northern latitude farmers are usually reluctant to adopt agroforestry as they fear that light competition will be fiercer in their conditions. We questioned the light availability for crops in alley-cropping at different latitudes from the tropic circle to the polar circle with a process-based 3D model of alley-cropping agroforestry. Two tree densities and two tree line orientations were considered. The effect of the latitude was evaluated with same-sized trees. The relative irradiance of the crops was computed for the whole year or at specific times of the year when crops need more light. The heterogeneity of crop irradiance across the alley was also computed. Surprisingly, crop relative irradiance of summer crops at high latitudes is high, at odds with farmers’ fears. Best designs were highlighted for improving the crop irradiance: North–South tree lines are recommended at high latitudes and East–West tree lines at low latitudes. At medium latitudes, North–South tree lines should be preferred to achieve an homogeneous irradiance of the crop in the alley. If we assume that trees at northern latitudes grow slower when compared to southern latitudes, then alley-cropping agroforestry is highly advisable even at high latitudes with summer crops.  相似文献   

Biocultural Diversity of Sarangani Province,Philippines: An Ethno-Ecological Analysis   总被引：1，自引：0，他引：1  

Florence Lasalita ZAPICO  Catherine Hazel AGUILAR  Angelie ABISTANO  Josephine Carino TURNER  Lolymar Jacinto REYES 《水稻科学》2015,22(3):138-146

This paper discussed effects of lowland-associated influences on upland ecology, food security and biocultural diversity in the Sarangani farming communities of the Philippines. In the uplands of Sarangani Province, the conservation of traditional rice varieties, the centrality of rice in tribal life, and the continued observance of planting rituals attest to its cultural significance and convey a common desire for cultural preservation and community solidarity. Economic and socio-political pressures had transformed tribal communities, although vestiges of traditional farming systems are still being practiced in remote sitios(villages). Changing land use patterns had also resulted in shrinking farm sizes and consequently in food insecurity in the Sarangani uplands. Extractive industries(i.e. logging, mining and charcoal making) and swidden farming were observed to cause widespread environmental degradation, while modern agriculture had undermined the capacity of indigenous peoples to survive because of their complete dependence on lands and resources. With the reality that cultural and biological diversities are inextricably linked, trans-disciplinary strategies coupling indigenous knowledge systems with scientific knowledge should, therefore, be instituted to save the Sarangani upland ecosystem, the indigenous peoples and their tribal resources.  相似文献   

Western Blot Analysis of Proacrosin/Acrosin in Frozen Dog Sperm During In Vitro Capacitation     

M De los Reyes  G Medina  J Palomino 《Reproduction in domestic animals》2009,44(S2):350-353

Acrosin is an acrosomal protease synthesized as an inactive precursor, proacrosin, which is processed via autoproteolysis into active forms alpha- and beta-acrosin. In this paper, a comparative study on the immunoreactivity of acrosin during in vitro capacitation of frozen and fresh (control) canine sperm using Western blot analysis is reported. Semen samples were obtained by digital stimulation and ejaculates processed as fresh and frozen samples and then capacitated for 0, 30, 60 and 90 min. At each time period, samples were analyzed with monoclonal antibody C5F10 by Western blot. The antibody specifically recognized, in fresh and frozen/thawed spermatozoa, a 40-, 32- and 27-kDa bands corresponding to proacrosin, alpha- and beta-acrosin, respectively, during capacitation. Western immunoblots showed that the beta-acrosin reactivity in fresh sperm was directly proportional to the time of capacitation, whereas a decreased reactivity of active form of acrosin was observed with frozen-thawed sperm (p   < 0.05). These results suggest that proacrosin is activated to beta-acrosin earlier in frozen/thawed dog spermatozoa than in fresh dog spermatozoa.  相似文献