Characterization of a monoclonal antibody to Spiroplasma eriocheiris and identification of a motif expressed by the pathogen     

Ying Zhang  Haixun Bao  Fengqin Miao  Yuqing Shen  Youjie He  Wei Gu  Qingguo Meng  Wen Wang  Jianqiong Zhang 《Veterinary microbiology》2013,161(3-4):353-358

Tremor disease (TD) has been found to be a lethal epidemic in Chinese mitten crab during recent years. A new species of spiroplasma, Spiroplasma eriocheiris (S. eriocheiris), was identified as the pathogen causing TD. In order to acquire appropriate biological tools for characterizing this newly discovered pathogen, we developed a new S. eriocheiris specific monoclonal antibody (mAb) 6H7. The antibody showed high binding affinity to S. eriocheiris (K_a = 6.25 × 10⁸ M^?1) and it bound to the adhesin-like protein (ALP41) of S. eriocheiris in Western blot analysis. Screening of a commercially available 12-mer linear peptide library by using 6H7 as bait led to isolation of a consensus sequence (FQGINHYNQMER). The clone displaying this sequence exhibited a significant, dose-dependent binding to 6H7 and inhibited the binding of S. eriocheiris to 6H7, suggesting a similar epitope between the sequence and S. eriocheiris. Homology searches and multiple sequence alignments indicated moderate homology between the consensus peptides and the ALP of S. eriocheiris. Thus, an internal image of 6H7 binding epitope might be buried in ALP41 of S. eriocheiris. In conclusion, 6H7 is a promising mAb to identify S. eriocheiris and the consensus sequence can be used in future research on the characterization of S. eriocheiris and its pathogenesis.  相似文献   

Comparative Analysis of the Acute Response of Zebrafish Danio rerio Skin to Two Different Bacterial Infections     

Aijun Lü  Xiucai Hu  Yi Wang  Xiaojing Shen  Aihua Zhu  Lulu Shen 《Journal of aquatic animal health》2013,25(4):243-251

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血清冻融次数对猪瘟抗体效价的影响   总被引：1，自引：0，他引：1  

钟锦辉  张海明  段晓冬  沈丹  朱建翠  邝惠仪 《广东畜牧兽医科技》2013,38(1):17-19

为研究反复冻融是否对猪瘟抗体效价产生影响,选取了60份不同抗体水平的猪瘟抗体阳性血清,每份血清分装6管作不同冻融处理。对不同冻融处理的血清进行物理性状观察和ELISA检测,并采用随机化区组设计资料秩和检验方法对检测OD值进行比较分析。结果发现不同次数冻融处理的血清物理性状观察无明显差异,ELISA检测OD值无统计学差异,表明短期内可完成ELISA检测的血清可直接于4℃冷藏保存或-20℃冻存,多次冻融不会造成血清猪瘟抗体效价的显著降低。  相似文献   

马病毒性动脉炎病毒中和试验的建立以及与ELISA方法的比较研究     

张海明  郑丽兰  段晓冬  相文华  沈丹  薛红  梁卓粤  彭南秀  程忠  彭聪 《中国动物检疫》2013,30(6):27-30

参照世界动物卫生组织(OIE)《陆生动物诊断与疫苗手册》,建立了马病毒性动脉炎病毒中和试验,并采用马病毒性动脉炎病毒中和试验(Virus Neutralization Test,VNT)和酶联免疫吸附试验(ELISA)两种方法,对广东省部分地区马场共182匹马血清和7份标准阴阳性血清进行马病毒性动脉炎抗体的检测,并对两种方法进行比较分析,结果两种方法的阳性符合率和阴性符合率分别为43.48%和96.34%,总符合率为93.12%;以我们建立的VNT作为马病毒性动脉炎抗体检测的"金标准",试验中所用的ELISA方法的敏感性和特异性分别为71.43%和93.96%。因此,我们认为在马病毒性动脉炎抗体检测中,ELISA的敏感性以及与VNT的阳性符合率较低,不适合作为VNT的替代方法。  相似文献   

饲粮粗蛋白质与外源褪黑激素水平对水貂生长性能、血清生化指标及营养物质消化率的影响     

宋兴超  薛海龙  陈秀敏  杨镒峰  魏海军  李光玉  杨福合 《动物营养学报》2013,(1):107-117

本试验旨在研究饲粮粗蛋白质(CP)和外源褪黑激素(MT)水平对水貂生长性能、血清生化指标及营养物质消化率的影响。选取90只50日龄体重一致的健康雄性水貂,随机分为6组,每组15个重复,每个重复1只水貂。采用3×2两因子试验设计,即3个饲粮CP水平(32%、36%和40%)和2个皮下埋植的外源MT水平(0、10 mg/只)。试验期64 d。结果表明:1)40%CP水平时,10 mg/只MT组水貂末重显著高于0 mg/只MT组(P<0.05);32%CP水平时,10 mg/只MT组水貂平均日增重显著高于0 mg/只MT组(P<0.05);饲粮CP和外源MT水平对水貂末重、平均日增重无显著互作效应(P>0.05)。2)32%和40%CP水平时,皮下埋植10mg/只MT可极显著降低水貂血清白蛋白(ALB)含量(P<0.01);32%CP水平的血清甘油三酯(TG)含量均显著高于40%CP水平(P<0.05);饲粮CP和外源MT水平对水貂血清ALB含量存在极显著互作效应(P<0.01),对血清总蛋白(TP)、尿素氮(UN)、TG含量和碱性磷酸酶(ALP)活性无显著互作效应(P>0.05)。3)10 mg/只MT组中,32%和36%CP水平的水貂干物质采食量极显著高于40%CP水平(P<0.01);0 mg/只MT组水貂中,32%CP水平的干物质采食量和干物质排出量极显著高于40%CP水平(P<0.01);饲粮CP和外源MT水平对水貂采食量、干物质采食量、干物质排出量和干物质消化率均无显著互作效应(P>0.05)。4)10 mg/只MT组中,36%CP水平的水貂粗脂肪消化率显著高于32%和40%CP水平(P<0.05),32%CP水平的水貂钙消化率极显著高于40%CP水平(P<0.01),32%CP水平的水貂磷消化率极显著高于36%和40%CP水平(P<0.01);0 mg/只MT组中,36%CP水平钙消化率极显著高于40%CP水平(P<0.01),32%CP水平的水貂磷消化率极显著高于36%和40%CP水平(P<0.01);饲粮CP和外源MT水平对水貂CP、粗脂肪、钙和磷的消化率无显著互作效应(P>0.05)。综合本试验各项测定指标可以得出,皮下埋植10 mg/只外源MT时,水貂较适宜的饲粮CP水平为32%～36%。  相似文献   

瘦肉型猪不同品种杂交组合生长性能比较分析     

段永邦  申学林  熊光源  蒲学栋  金鸿 《贵州畜牧兽医》2013,37(1):1-3

选择健康无病、体况良好、体重相近(24±1.27 kg)的长×大、巴×长大、杜×长大断奶仔猪各10头,共计30头,分为3组,研究不同杂交组合间的生长性能。3组试验猪全部饲喂常规日粮。结果显示,与长×大组相比,巴×长大、杜×长大组猪平均采食量降低4.97%(P>0.05)、5.47%(P>0.05),平均日增重提高3.13%(P<0.05)、1.14%(P<0.05),料重比下降7.94、6.35%,头均经济效益增加0.61、0.41元;结果表明,巴×长大和杜×长大组的生产性能较好,值得在生产中推广。  相似文献   

“粉末炭活性污泥法+Fenton试剂”强化处理抗生素生产废水的研究     

李赟  魏胜军  高彦龙  沈博城 《绿色科技》2013,(6):143-144

采用"粉末炭活性污泥法+Fenton试剂"强化处理抗生素生产废水,与"普通活性污泥法"进行了对比试验。结果表明:"普通活性污泥法"处理抗生素生产废水后有机污染物COD平均浓度为759mg/L,平均去除率89.1%,达不到该药厂废水排放标准,而"粉末炭活性污泥法+Fenton试剂"强化处理抗生素生产废水后有机污染物COD平均浓度186mg/L,平均去除率为97.3%,完全达到该药厂排放标准。  相似文献   

Elevated cyclin D1 expression is governed by plasma IGF‐1 through Ras/Raf/MEK/ERK pathway in rumen epithelium of goats supplying a high metabolizable energy diet     

J. Lu  H. Zhao  J. Xu  L. Zhang  L. Yan  Z. Shen 《Journal of animal physiology and animal nutrition》2013,97(6):1170-1178

The objective of this study was to explore the underlying mechanism of insulin‐like growth factor 1 (IGF‐1)–caused cell proliferation of rumen epithelium in goats fed a high metabolizable energy (ME) diet. In this study, young goats were fed either a low ME [LL, n = 9, ME: 0.57 MJ/kg^0.75/day] or high ME [HL, n = 9, ME: 1.00 MJ/(kg^0.75/day)] diet for 42 day. The time duration of G₁‐phase was shortened as a result of enhanced expression of cyclin D1 mRNA in the HL group (p < 0.05). It was suggested that a high ME diet promoted cell transition from G₀/G₁ to S‐phase via cyclin D1. The level of phosphorylation of ERK was higher in HL than LL group (p < 0.05). In cell culture, the ERK was phosphorylated by IGF‐1 treatment. The proliferative effects of insulin‐like growth factor 1 (IGF‐1, 25 ng/ml) on [³H] thymidine (TdR) incorporation into DNA and on cyclin D1 protein expression of rumen epithelial cells were inhibited by PPP (the inhibitor of type 1 IGF receptor) (p < 0.05) and ERK inhibitor (p < 0.05) in vitro. Thus, IGF‐1 up‐regulated cyclin D1 expression and accelerated G₁‐phase progression in the cell cycle through Ras/Raf/MEK/ERK pathway in rumen epithelium of goats.  相似文献   

Improving efficiency of sow productivity： nutrition and health   总被引：2，自引：0，他引：2  

Sung Woo  Kim Alexandra  C  Weaver Yan  Bin Shen  Yan Zhao 《畜牧与生物技术杂志(英文版)》2013,(3):187-194

This reviews research focused to understand the nutrient requirement and balance to meet the needs of fetal growth,mammary growth,and milk production.This summary will handle how feeding strategies can be adjusted according to the nutrient needs for a sow to enhance productivity and health.Most research data used in this summary are based on the studies conducted by the authors between 1996 and 2013.Nutrient requirements of sows are affected by stage of gestation and parity of sows.Dietary antioxidant concentrations need to be re-evaluated for its sufficiency in sow diets especially to prevent excessive oxidative stress during late gestation and lactation.When feeding sows,consideration of phase feeding of gestating sows and parity feeding of lactating sows could enhances production longevity and health of sows.Use of selected nutrients and additives seems to help productivity and health of sows.  相似文献   

冈仓天心《茶之书》与时代之研究     

沈冬梅 《农业考古》2019,(2):222-231

《茶之书》是冈仓天心"文明论三部曲"中最有影响的英文著作,成书出版于甲午战争、日俄战争中日本获胜之后,当时日本被西方逼迫开国进而主动维新进行改革,因对华、对俄战争的胜利得到西方世界认可,随之而起的是文化上的自觉、自信乃至文化观念输出的冲动。冈仓天心用"以日本为中心的东亚艺术史"构造亚洲一体论,构建近代日本的国际定位,通过作为"一种唯美的信仰"的茶道让世界认识亚洲文化特别是日本文化,为日本树立"和平"形象。  相似文献