Effect of heparin on a haemagglutinin of porcine reproductive and respiratory syndrome virus     

E.R Jusa  Y Inaba  M Kong  T Asagoe  K Uwatoko  O Hirose 《Research in veterinary science》1997,62(3):261

Heparin inhibited haemagglutination by porcine reproductive and respiratory syndrome virus (PRRSV) and by Aujesky's disease virus, but failed to inhibit haemagglutination by parainfluenza virus type 3. The minimal inhibitory concentration of heparin required to inhibit 8 HA U of PRRSV haemagglutinin ranged from 0·1 to 1 U ml⁻¹. Mouse erythrocytes failed to combine with the haemagglutination inhibitory factor of heparin. However, mouse erythrocytes treated with heparinase had greatly reduced agglutinability by PRRSV. The formation of a haemagglutinin-heparin complex could be observed by sedimenting heparin with the haemagglutinin. All these findings suggest that a heparin-like molecule on the surface of mouse erythrocytes serves as the virus-cell receptor.  相似文献   

Reduction of mucin-1 gene expression associated with increased Escherichia coli adherence in the canine uterus in the early stage of dioestrus     

Ishiguro K  Baba E  Torii R  Tamada H  Kawate N  Hatoya S  Wijewardana V  Kumagai D  Sugiura K  Sawada T  Inaba T 《Veterinary journal (London, England : 1997)》2007,173(2):325-332

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Effect of danazol on NK cells and cytokines in the mouse uterus     

Kusakabe K  Morishima S  Nakamuta N  Li ZL  Otsuki Y 《The Journal of reproduction and development》2007,53(1):87-94

Danazol, which has been used as a medicine for endometriosis, has a valid effect in pretreatment of patients receiving in vitro fertilization and embryo transfer, although its reproductive mechanism remains unclear. BALB/c mice were subcutaneously injected with danazol for 2 weeks. Blood and uteri were collected and cytokines were assayed. Following danazol treatment, an increase in pregnancy ratio was evident that was accompanied by up-regulation in serum macrophage-colony stimulating factor (M-CSF). RT-PCR analysis revealed that expression of M-CSF and Ly49, a phenotypic marker of natural killer (NK) cells, was up-regulated in the uteri of the danazol-treated mice. In immunohistochemical analysis, M-CSF and Ly49, together with alpha5 integrin, were clearly detected in the endometrium of the danazol-treated mice with very similar pattern of localization. These results suggest that danazol has an effect to promote pregnancy that induces recruitment of NK cells and a concomitant increase in the expression of M-CSF and alpha5 integrin in the uterus.  相似文献   

Antioxidant mechanism of carnosic acid: structural identification of two oxidation products.   总被引：1，自引：0，他引：1  

T Masuda  Y Inaba  Y Takeda 《Journal of agricultural and food chemistry》2001,49(11):5560-5565

To determine the antioxidant mechanism of food phenolics against the oxidation of food components, the reaction of carnosic acid, an antioxidative constituent of the popular herbs sage and rosemary, was investigated in the presence of ethyl linoleate and the radical oxidation initiator 2,2'-azobis(2,4-dimethylvaleronitrile). During this process, carnosic acid was oxidized to an o-quinone and a hydroxy p-quinone, the chemical structures of which were confirmed by physical and chemical techniques. From a quantitative time course analysis of the production of these quinones, an antioxidant mechanism of carnosic acid is proposed, consisting of the oxidative coupling reaction with the peroxyl radical at the 12- or 14-position of carnosic acid and subsequent degradation reactions.  相似文献   

Recovery mechanism of the antioxidant activity from carnosic acid quinone,an oxidized sage and rosemary antioxidant     

Masuda T  Inaba Y  Maekawa T  Takeda Y  Tamura H  Yamaguchi H 《Journal of agricultural and food chemistry》2002,50(21):5863-5869

A solution of carnosic acid quinone, which is a radical chain-termination product having no antioxidant activity in the antioxidant reaction of carnosic acid, recovers potent antioxidant activity upon standing. The HPLC analysis of an aged solution of carnosic acid quinone revealed that several antioxidants are produced in the solution. From the time-course and quantitative analyses of the formation of the products and their structural analysis, an antioxidant mechanism from carnosic acid quinone is proposed that includes a redox reaction of carnosic acid quinone in addition to the isomerization to lactone derivatives. In the first stage of antioxidation, carnosic acid, the reduction product from carnosic acid quinone, contributes to the potent antioxidant activity of the solution. This proposed mechanism can explain one of the reasons for the strong antioxidant activity of the extract of the popular herbs sage and rosemary.  相似文献   

The formation process of button ulcers in pigs experimentally infected with a subgenotype 2.1 isolate of classical swine fever virus     

Kasumi SUDO  Manabu YAMADA  Mariko OCHIAI  Shoko IWAMOTO  Hajime SAKAKI  Chiho KUSHIDA  Maiko YAMASHITA  Atsushi YAMAMOTO  Takao NAGASAKA  Mikio OIDEMIZU  Akito SAITO  Kinya YAMAMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(6):990

We evaluated the role of classical swine fever virus (CSFV) in the formation of button ulcers in the mucosa of the gastrointestinal tract. Histopathological and immunohistochemical analyses of pigs experimentally infected with a subgenotype 2.1 isolate of CSFV, which was isolated in Japan in 2019, revealed follicular necrosis in the submucosal mucosa-associated lymphoid tissue and herniation of crypts as factors that contribute to the development of button ulcers during CSFV infection. These findings indicate that CSFV induces follicular necrosis and is one of the causative agents of button ulcers in pigs.  相似文献   

Underlying mechanisms involved in the decrease of milk secretion during Escherichia coli endotoxin induced mastitis in lactating mice     

Ken Kobayashi  Shoko Oyama  Takaaki Uejyo  Chinatsu Kuki  Md Morshedur Rahman  Haruto Kumura 《Veterinary research》2013,44(1):119

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Maternal high‐fat diet promotes onset of diabetes in rat offspring          下载免费PDF全文

Takeshi Ohta  Yasufumi Toriniwa  Naruhiko Ryumon  Nobuhiro Inaba  Tadaaki Hirao  Saori Yamanaka  Takayuki Maeno  Wakako Sakakibara  Morio Sumikawa  Kaoru Chiba  Akiko Nakamura  Katsuhiro Miyajima  Fatchiyah Fatchiyah  Takahisa Yamada 《Animal Science Journal》2017,88(1):149-155

The onset and progression of type II diabetes is closely related to environmental factors, in particular dietary habit. Moreover, the environmental exposures very early in life can influence the risk for development of type II diabetes later in life. In this study, we investigated pathophysiological changes in the pups of maternal Spontaneously Diabetic Torii (SDT) rats that were fed a high‐fat diet (HFD) throughout gestation and lactation. Maternal SDT rats were continued on HFD for 5 weeks, from day 8 of gestation to day 21 after birth, and biological analyses of the pups were performed from 2 to 22 weeks of age. Results of serum lipid levels in pups from dams fed HFD were higher than pups from dams fed a standard diet, and the onset of diabetes was significantly accelerated in pups from dams fed HFD. In pathological analyses, pups from dams fed HFD showed increases in liver weight and vacuolation of hepatic cells at 2 weeks of age. In conclusion, the metabolic disorder of lipids and glucose in SDT rats is closely related to the nutritional condition of dams during the periods of gestation and lactation.  相似文献   

Hypothalamic dopamine is required for salsolinol‐induced prolactin secretion in goats          下载免费PDF全文

Tsutomu Hashizume  Ryunosuke Watanabe  Yuki Inaba  Ken Sawai  Ferenc Fülöp  György Miklos Nagy 《Animal Science Journal》2017,88(10):1588-1594

The aim of the present study was to clarify the relationship between hypothalamic dopamine (DA) and salsolinol (SAL) for the secretion of prolactin (PRL) in goats. SAL or thyrotropin‐releasing hormone (TRH) was intravenously injected into female goats treated with or without the D₂ DA receptor antagonist haloperidol (Hal), which crosses the blood‐brain barrier, and the PRL‐releasing response to SAL was compared with that to TRH. PRL‐releasing responses to SAL, Hal, and Hal plus SAL were also examined after a pretreatment to augment central DA using carbidopa (Carbi) and L‐dopa. The PRL‐releasing response to Hal alone was greater than that to SAL or TRH alone. The PRL‐releasing response to Hal plus SAL was similar to that of Hal alone. In contrast, the PRL‐releasing response to Hal plus TRH was greater than that to TRH or Hal alone. The treatment with Carbi plus L‐dopa inhibited SAL‐ and Hal‐induced PRL secretion. The inhibition of the PRL‐releasing response to SAL disappeared when SAL was injected with Hal. These results indicate that the mechanisms underlying the SAL‐induced PRL response differ from those of TRH, and suggest that hypothalamic DA and its synthesis is associated in part with SAL‐induced PRL secretion in goats.  相似文献   

Effects of dietary heme iron and exercise training on abdominal fat accumulation and lipid metabolism in high‐fat diet‐fed mice          下载免费PDF全文

Masanori Katsumura  Shoko Takagi  Hana Oya  Shohei Tamura  Takaoki Saneyasu  Kazuhisa Honda  Hiroshi Kamisoyama 《Animal Science Journal》2017,88(8):1100-1106

Animal by‐products can be recycled and used as sources of essential nutrients. Water‐soluble heme iron (WSHI), a functional food additive for supplementing iron, is produced by processing animal blood. In this study, we investigated the effects of dietary supplementation of 3% WSHI and exercise training for 4 weeks on the accumulation of abdominal fat and lipid metabolism in mice fed high‐fat diet. Exercise‐trained mice had significantly less perirenal adipose tissue, whereas WSHI‐fed mice tended to have less epididymal adipose tissue. In addition, total weight of abdominal adipose tissues was significantly decreased in the Exercise + WSHI group. Dietary WSHI significantly increased the messenger RNA (mRNA) levels of lipoprotein lipase and hormone‐sensitive lipase. WSHI‐fed mice also tended to show increased mRNA levels of adipose triglyceride lipase in their epididymal adipose tissue. Dietary WSHI also significantly decreased the mRNA levels of fatty acid oxidation‐related enzymes in the liver, but did not influence levels in the Gastrocnemius muscle. Exercise training did not influence the mRNA levels of lipid metabolism‐related enzymes in the epididymal adipose tissue, liver or the Gastrocnemius muscle. These findings suggest that the accumulation of abdominal fat can be efficiently decreased by the combination of dietary WSHI and exercise training in mice fed high‐fat diet.  相似文献