Evaluation of Intestinal Absorption of Dietary Halocynthiaxanthin,a Carotenoid from the Sea Squirt Halocynthia roretzi     

Chiaki Ikeda  Yuki Manabe  Nami Tomonaga  Tatsuya Wada  Takashi Maoka  Tatsuya Sugawara 《Marine drugs》2020,18(12)

Halocynthiaxanthin is an acetylenic carotenoid mainly found in Halocynthia roretzi. To date, several bioactivities of halocynthiaxanthin have been reported, but its mechanism of digestion and absorption in mammals has not been studied yet. In this study, we evaluated the intestinal absorption of halocynthiaxanthin in mice. The halocynthiaxanthin-rich fraction was prepared from the tunicate Halocynthia roretzi. Mice were orally administered the fraction at a dose of 5 mg/kg body weight. The halocynthiaxanthin levels in the plasma, liver, and small intestine, were quantified using HPLC-PDA, 1, 3, 6, and 9 h after ingestion. The halocynthiaxanthin-rich fraction mainly consisted of the all-trans form and a small amount of cis forms. These three isomers were detected in the plasma of mice 3 h after ingestion. Time-course changes after the ingestion of this fraction were found, with cis isomers being more abundant than the all-trans isomer in the mouse plasma and liver. In the small intestine, however, the all-trans isomer was primarily detected. The possibility that cis isomers might be absorbed rapidly from the small intestine cannot be denied, but our results suggest that dietary all-trans-halocynthiaxanthin might be isomerized to the cis isomer after intestinal absorption.  相似文献   

Parathyroid hormone‐related protein‐induced hypercalcemia due to osteosarcoma in a cat          下载免费PDF全文

Masashi Yuki  Makiko Nitta  Tetsuo Omachi 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2015,44(1):141-144

A 15‐year‐old castrated male mixed‐breed cat was presented with a history of sarcoma of the distal right hind limb. Biochemical analysis revealed increased concentrations of blood urea, creatinine, total calcium, ionized calcium, and parathyroid hormone‐related protein (PTHrP). The mass was removed surgically by amputation of the hind limb. Osteosarcoma was diagnosed based on histopathologic examination. All abnormal serum analyte concentrations improved immediately after surgery, including azotemia, total calcium, ionized calcium, and PTHrP. The biochemical results were attributed to osteosarcoma causing PTHrP‐induced hypercalcemia.  相似文献   

Time course of the incidence/multiplicity and histopathological features of murine colonic dysplasia,adenoma and adenocarcinoma induced by benzo[a]pyrene and dextran sulfate sodium     

Jiro Sonoda  Yuki Seki  Atsushi Hakura  Satoru Hosokawa 《Journal of toxicologic pathology》2015,28(2):109-120

Benzo[a]pyrene (BP) is mutagenic but noncarcinogenic in the murine colon. Recently, we reported rapid induction of colonic tumors by treatment of CD2F₁ mice with BP (125 mg/kg for 5 days) followed by a colitis inducer, dextran sulfate sodium (DSS) (4% in drinking water for 1 or 2 weeks). However, there are no reports on detailed time course and histopathological features of colonic proliferative lesions in this model. Here, we show the detailed time course of colonic dysplasia, adenoma and adenocarcinoma induced by treatment with BP, DSS, and a combination of the two (BP/DSS). In the colon of mice exposed to BP/DSS, 14.6 dysplastic foci per mouse were present one week after DSS treatment (week 4). The number of dysplastic foci decreased with time to 3.1 at week 9 and thereafter remained almost constant. At week 4, 1.5 adenocarcinomas were also observed, with a marked increase in numbers with time, reaching 29.3 at week 14. In contrast, the number of dysplastic foci induced by DSS alone showed a time course similar to that following BP/DSS treatment; however, only a few tumors appeared. Neither dysplastic foci nor neoplastic lesions were induced by BP only. In mice exposed to BP/DSS, β-catenin was demonstrated immunohistochemically in the nucleus and/or cytoplasm of the tumor cells, and this translocation from the cell membrane was evident in subsets of dysplastic foci. In dysplastic foci induced by DSS alone, β-catenin was absent in the nucleus/cytoplasm. These finding suggest that aberrant β-catenin accumulation in dysplastic foci is associated with tumor progression in this BP/DSS model.  相似文献   

Identification of immunodiagnostic antigens for cerebrospinal filariasis in horses by western blot analysis     

Masataka TAKESUE  Yuki OSAKA  Masanori MURANAKA  Yoshinari KATAYAMA  Hiromi IKADAI 《Journal of Equine Science》2016,27(1):17-18

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Formation of optically active neolignans from achiral coniferyl alcohol by cell-free extracts of Eucommia ulmoides     

Takeshi Katayama  Yuki Kado 《Journal of Wood Science》1998,44(3):244-246

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Effects of chronic acid fog exposure with soil acidification or nitrogen loading on nutrient status in Abies firma seedlings     

Koji Yoshida  Shoko Inaba  Chisato Takenaka  Takafumi Tezuka 《Journal of Forest Research》2005,10(2):157-161

To clarify the nutrient status in momi fir (Abies firma Sieb. et Zucc.) seedlings under complex stress conditions of acid fog with soil acidification or nitrogen loading, we exposed seedling shoots to simulated acid fog (pH 3) and simultaneously loaded the soil with acid or excess nitrogen for 17 months. Proton and nitrogen loading reduced K concentration in soil, but these treatments had little effect on the nutrient status of fine roots in momi fir seedlings. Acid fog exposure resulted in reduced concentrations of Mg, Al, and Fe in current-year needles and Mg, Al, Cu, Fe, and Zn in 1-year-old needles. The complex effects of acid fog exposure with proton or nitrogen loading on nutrient status were relatively slight. However, elements such as Al and Cu notably reflect the effects of proton loading. These results show that the nutrient status of trace elements, rather than major elements, in needles of momi fir seedlings is sensitive to exposure to acid fog or proton loading.  相似文献   

A probabilistic eco-hydrological model to predict the effects of climate change on natural vegetation at a regional scale     

Jan-Philip M. Witte  Ruud P. Bartholomeus  Peter M. van Bodegom  D. Gijsbert Cirkel  Remco van Ek  Yuki Fujita  Gijs M. C. M. Janssen  Teun J. Spek  Han Runhaar 《Landscape Ecology》2015,30(5):835-854

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Quercetin-4'-glucoside is more potent than quercetin-3-glucoside in protection of rat intestinal mucosa homogenates against iron ion-induced lipid peroxidation     

Murota K  Mitsukuni Y  Ichikawa M  Tsushida T  Miyamoto S  Terao J 《Journal of agricultural and food chemistry》2004,52(7):1907-1912

Quercetin is a typical antioxidative flavonoid found in vegetables, which is more commonly present as its glucosides, quercetin-3-glucoside (Q3G) and quercetin-4'-glucoside (Q4'G). The main aim of this study was to estimate the antioxidant activity of Q3G and Q4'G on iron ion-driven lipid peroxidation of the gastrointestinal mucosa. Q4'G markedly suppressed the lipid peroxidation when rat gastrointestinal mucosa homogenates were incubated with Fe(NO3)3 and ascorbic acid. Its effectiveness was greater as compared to that of Q3G and comparable to that of quercetin aglycone. Furthermore, Q4'G yielded higher amounts of quercetin aglycone than Q3G on incubation with the homogenates. However, Q4'G showed a lower chelating activity in comparison to Q3G. These results indicate that Q4'G, even though it has a low chelating activity, because of its efficient conversion to antioxidative aglycone on exposure to the mucosa, can act as a powerful antioxidant on iron ion driven lipid peroxidation in the intestinal mucosa. Thus, vegetables rich in Q4'G, such as onion, are likely to serve as favorable antioxidant sources for suppressing iron-induced oxidative stress in the intestinal tract.  相似文献   

α-Melanocyte-stimulating hormone directly increases the plasma calcitonin level and involves calcium metabolism in goldfish     

Ikari  Takahiro  Kobayashi  Yuki  Kitani  Yoichiro  Sekiguchi  Toshio  Endo  Masato  Kambegawa  Akira  Asahina  Kiyoshi  Hattori  Atsuhiko  Tabuchi  Yoshiaki  Amornsakun  Thumronk  Mizusawa  Kanta  Takahashi  Akiyoshi  Suzuki  Nobuo 《International Aquatic Research》2018,10(3):283-292

International Aquatic Research - The effects of α-melanocyte-stimulating hormone (α-MSH) on calcium metabolism were examined with goldfish. The scales on the left side of goldfish bodies...  相似文献   

Correction to: A new and simple method for measuring in situ field-saturated hydraulic conductivity using a falling-head single cylinder     

Noborio  Kosuke  Ito  Yuki  He  Hailong  Li  Min  Kojima  Yuki  Hara  Hirofumi  Mizoguchi  Masaru 《Paddy and Water Environment》2018,16(1):81-87

Paddy and Water Environment - Hydraulic properties of soil play important roles in water and temperature regimes. Measuring hydraulic properties has been studied for decades in the laboratory and...  相似文献