体细胞克隆荷斯坦奶牛出生后死亡犊牛内脏器官的组织病理学观察     

孙伟  包向男  乌云高娃  王建国  李永胜  李善铎  张铁柱  王飞飞  李喜和 《中国农学通报》2020,36(20):114-118

为探究体细胞克隆荷斯坦奶牛出生后死亡的原因,对新生后死亡的克隆荷斯坦公牛和自然繁殖的荷斯坦公犊的主要组织器官进行比较。通过解剖和石蜡切片-HE(hematoxylin-eosin staining)染色技术,对主要的组织器官结构进行观察和分析。结果表明,新生后死亡的克隆荷斯坦公牛肺部结构清晰;肝脏的肝细胞明显肿大,出现脂肪轻度变性;肾小管上皮细胞出现变性;心肌的肌纤维间空隙增大;骨骼肌纤维间隙明显,空泡变性,这可能导致该克隆牛犊肌肉无力并功能不全;淋巴结皮髓质界限不分明,淋巴小结细胞较稀疏,生发中心不明显,淋巴窦细胞较少;脾脏内红细胞较少,这说明该克隆牛的造血功能可能不完善;胸腺的皮质部分和髓质部分界限不明显,嗜酸性胸腺小体不易辨认,可能发育不全。该克隆公牛免疫器官出现不同程度的发育不全现象较严重,这有可能是其出生后死亡率高的主要原因。  相似文献   

进出境动物及动物产品风险分析     

王照修 《中国动物检疫》2003,20(7):11-11

加入WTO ,为我国经济发展提供了有利的发展机遇 ,但我国在对外贸易活动中却遭遇到技术性贸易壁垒 ,影响我国对外经济活动 ,使不少企业蒙受损失。为提高自身素质和了解国内外进出境动物及动物产品方面的知识 ,本人搜集了一些关于进出境动物及动物产品风险分析知识 ,现整理出来与同仁们共同学习。国际间动物和动物产品的贸易有很大的特殊性和复杂性。一方面 ,引进国外动物优良品种可以提高本国动物生产性能和质量 ,促进畜牧业生产发展和人民生活水平的提高 ;另一方面 ,动物的交换及动物产品的流通则有将国外动物传染病和寄生虫病传入的危险 …  相似文献   

Long-term study of aerobic bacteria of the genital tract in stud dogs.     

L Bjurstr?m  C Linde-Forsberg 《American journal of veterinary research》1992,53(5):670-673

The aerobic bacterial flora of the genital tract was characterized in 15 stud dogs in an 18-month study. The dogs represented 4 breeds and were from 3 kennels. Bacterial samples from the prepuce and semen were collected every month, except in connection with matings, when they were collected weekly (464 samples). The dogs that were included all mated at least once during the study. The mean pregnancy rate, litter size, and pup mortality for the bitches with which they had mated were all within normal limits. The most frequent bacteria isolated from the prepuce and semen were Pasteurella multocida, beta-hemolytic streptococci, and Escherichia coli. There was a tendency for breeds to differ in frequency of the most common bacterial species. Bacterial culture yielded no aerobic growth in 14.2% of the preputial samples and 69.8% of the semen samples. Bacteria were transferred between dog and bitch at mating. In this study of healthy breeding dogs, neither the fertility of the dog nor that of the bitch was affected by the bacteria transferred.  相似文献   

Food safety paramount in '90s.     

L Spencer 《Journal of the American Veterinary Medical Association》1992,200(9):1191-1196

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Clinical response of nasal adenocarcinoma to cisplatin chemotherapy in 11 dogs.     

K A Hahn  D W Knapp  R C Richardson  C L Matlock 《Journal of the American Veterinary Medical Association》1992,200(3):355-357

In 11 dogs with nasal adenocarcinoma, IV treatment with 2 to 8 cycles of cisplatin at a dosage of 60 mg/m2 of body surface, given at 3-week intervals, resulted in a survival time of 8 to 128 weeks (mean, 32.7 weeks; median, 20 weeks). Radiography revealed complete remission in 2 dogs and partial remission in 1 dog, for an overall response rate of 27%. Clinical problems such as nasal discharge (n = 11), sneezing (n = 6), and epistaxis (n = 4) resolved in 1 to 2 weeks in all dogs after beginning chemotherapy.  相似文献   

Factors affecting skim milk progesterone assay results.     

R F Nachreiner  S J Oschmann  L E Edqvist  J I Richards 《American journal of veterinary research》1992,53(7):1085-1089

Five studies were performed to determine factors affecting progesterone concentration in skim milk. Results of the first study indicated that progesterone concentration was higher in skim milk of samples kept 16 hours in an ice bath (0 C) than of those left at room temperature (21 C). In the second study, this temperature effect was found to be reversible, with skim milk progesterone concentration increasing when whole milk samples were cooled prior to centrifugation. In the third study, [3H]-labeled progesterone was used to determine the relationship between fat content of foremilk (the first milk obtained from the teats), midmilk (milk obtained midway through milking), and strippings (milk obtained immediately after milking machines have been removed) samples and temperature (4 C and 21 C) on the percentage of progesterone in the skim milk fraction. The relationship between percentage of butterfat and percentage of progesterone in skim milk was linear when the log of these variables was used for calculations. In the fourth study, assayable progesterone in the skim milk fraction of foremilk, midmilk, and strippings was affected by temperature. In the fifth study, a multiple-regression procedure was used to determine the amount of variation in percentage of radioactive progesterone in the skim milk fraction. Independent variables (whole milk butterfat and temperature of incubation [1, 3, 13, 22, 37, and 50 C]) and the natural log of each variable, were entered into a stepwise multiple-regression analysis.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Bovine spongiform encephalopathy: epidemiological features 1985 to 1990.   总被引：4，自引：0，他引：4  

J W Wilesmith  J B Ryan  W D Hueston  L J Hoinville 《The Veterinary record》1992,130(5):90-94

Following the identification of bovine spongiform encephalopathy (BSE) in the British cattle population in 1986 epidemiological studies were launched. This paper provides an updated account of the epidemiological features of BSE from 1985, when the first cases, based on clinical histories, occurred, until 1990. The number of cases up to December 1989 represents an annual incidence of 3.9 confirmed cases per 1000 adult animals in Great Britain. Many more dairy herds were affected than beef suckler herds, a difference attributable to the difference in feeding practices between the two herd types. The geographical variation in incidence previously described has persisted with the highest incidence in the south and east of England. Other features of the epidemiology, including the low within-herd incidence, remained unaltered from the earlier findings. The results support the previously suggested hypothesis that the outbreak of BSE was due to the sudden exposure of the cattle population to a scrapie-like agent in 1981/82. There was no evidence of direct transmission between cattle during the period considered.  相似文献   

Blindness associated with toxoplasmosis in canaries.     

M C Vickers  W J Hartley  R W Mason  J P Dubey  L Schollam 《Journal of the American Veterinary Medical Association》1992,200(11):1723-1725

Seven of 30 canaries in an aviary in New Zealand developed ophthalmic problems. Clinically, 5 birds had unilateral and 2 birds had bilateral lesions characterized by conjunctivitis, crusty exudates on eyelids, and collapse of the eyeball. Microscopic lesions in 12 of 14 eyes examined included inflammation of the choroid and retina, with osseous replacement of the globe in some. Numerous Toxoplasma gondii tachyzoites were seen in the detached retina and vitreous humor of acutely affected birds. The diagnosis of toxoplasmosis was confirmed by immunohistochemical staining with T gondii antiserum. Affected birds had encephalitis, and T gondii was localized in the brains of these by immunohistochemical examination and by use of bioassays in mice. Toxoplasmosis should be considered in differential diagnosis of ophthalmitis in canaries.  相似文献   

秦岭山区地被植物种质资源研究初报   总被引：1，自引：1，他引：0  

陈新露  赵祥云 《草业科学》1993,10(4):11-14

本文报道了秦岭山区地被植物的种类、产地、生态习性、形态特征及园林中的应用。  相似文献   

一类耗散非线性波动方程初边值问题整体解的存在与非存在性     

范恩贵  王静海 《河北北方学院学报(自然科学版)》1994,(2)

本文考虑一类耗散非线性波动方程的初边值问题。用半(?)方法和先验估计论证了该问题小初值下在时间大范围的可解性、唯一性;并用凸性方法证明了(?)当条件下该问题的解在有限时间内发生爆破现象。  相似文献