Detection of novel polymorphisms in the ckit gene of canine patients with lymphoma,melanoma, haemangiosarcoma,and osteosarcoma     

Irina Gramer  Martin Kessler  Joachim Geyer 《Veterinary research communications》2016,40(2):89-95

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Immunological and pathological investigations in equine experimental uveitis     

Galina P. Simeonova  Svetozar Z. Krastev  Radostin S. Simeonov 《Veterinary research communications》2016,40(3-4):107-115

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苹果园几种鸟雀驱避技术效果研究     

李莉  王春良  李晓龙  贾永华  王永忠  潘志广 《北方园艺》2016,(1):19-22

选择种有"美国八号"(早熟)、"嘎啦"(中熟)、"富士"(晚熟)的混栽果园4片(各2/3hm~2)为研究对象,设置4个处理(分别为有色防鸟网、风动叶轮、超声波、生物驱避剂),对比了4种鸟雀驱避技术,调查其驱避效果,以筛选出最优的鸟雀驱避技术。结果表明:在相同品种条件下,4种鸟雀驱避技术防鸟害的有效程度以有色防鸟网驱避效果最好,持效性最长,稳定性最好;其受啄率分别为"美国八号"0.44%、"嘎啦"0.16%、"富士"0.20%。其次为生物驱避剂,其受啄率分别为"美国八号"1.92%、"嘎啦"2.24%、"富士"1.52%。然后为超声波驱避技术,其受啄率分别为"美国八号"9.64%、"嘎啦"5.56%,"富士"3.24%。驱避效果最差、持效性最短、稳定性最差的是风动叶轮驱鸟,其受啄率分别为"美国八号"10.00%、"嘎啦"6.52%、"富士"2.60%。3个品种综合调查结果表明,早熟苹果受啄率最高,其次是中熟品种,受啄率最低的是晚熟品种。  相似文献   

喷钙及不同贮藏处理对‘金冠’苹果保鲜效果的影响     

王晓佳  贾永华  李晓龙  王春良 《北方园艺》2016,(11):138-141

以‘金冠’苹果为试材,研究了喷施不同钙肥的苹果在聚乙烯保鲜膜包装(MAP)、果蜡涂膜、壳聚糖涂膜等不同贮藏条件下果实贮藏品质的变化。结果表明:‘金冠’苹果在不同贮藏方式下,随贮藏时间的延长,果实的腐烂、失重、硬度降低,可溶性固形物、可滴定酸含量的下降程度均有一定的延缓效果,同时抑制了维生素C的降解。适宜的贮藏处理对苹果果实具有良好的保鲜作用,其中1%浓度的壳聚糖处理效果最好。  相似文献   

北虫草菌种复壮方法的比较     

王殿振  贺晓龙  赵瑞华  任桂梅  刘月芹 《北方园艺》2016,(17):137-139

以已退化的北虫草菌种为试材,采用孢子分离、组织分离、活体蚕蛹、基内菌丝分离、蚕蛹回接等5种菌种复壮法,研究了不同复壮方法对退化菌种复壮效果的影响。结果表明:经活体蚕蛹复壮法得到的菌种在菌丝生长速度和子实体产量均是最高的,菌丝生长速度提高了29.97%,子实体产量提高了383.93%,故确定活体蚕蛹复壮法是5种方法中最好的虫草菌种复壮法。  相似文献   

Development and evaluation of enzyme-linked immunosorbent assay based on recombinant nucleocapsid protein for detection of porcine epidemic diarrhea (PEDV) antibodies   总被引：3，自引：0，他引：3  

Hou XL  Yu LY  Liu J 《Veterinary microbiology》2007,123(1-3):86-92

An enzyme-linked immunosorbent assays (ELISA) based on recombinant nucleocapsid (N) protein generated in Escherichia coli was evaluated for its sensitivity and specificity for diagnosis of porcine epidemic diarrhea (PEDV) infection. The N gene encoding the N protein was cloned and expressed as a fusion protein with His tag protein in E. coli. The recombinant N protein was migrated at 48 kDa and reacted with six histidine tag specific monoclonal antibody by immunoblotting. Recombinant N protein ELISA (rnELISA) demonstrated 98.7% specificities among (80) PEDV-free individuals, and 98% sensitivity ranging among (103) clinical samples with PEDV. On testing 884 field samples, an overall agreement of 88.3% was generated between the SN and rnELISA. Taken together, these results indicated that nucleocapsid protein may be a useful antigen for the sera-diagnosis of PEDV and it was also suggested that the ELISA is a highly sensitive and specific test for detecting antibodies to PEDV.  相似文献   

Stepwise in-straw dilution and direct transfer using open pulled straws (OPS) in the mouse: a potential model for field manipulation of vitrified embryos     

Yang QE  Hou YP  Zhou GB  Yang ZQ  Zhu SE 《The Journal of reproduction and development》2007,53(2):211-218

In the present study, mouse blastocysts were employed to investigate the feasibility and efficiency of stepwise in-straw dilution and direct transfer using the open pulled straw (OPS) method. In experiment I, the effects of various vitrification solutions (VS) on embryo survival were examined. After thawing, the expanded blastocyst rates (97.59 and 95.05%) and hatching rates (80.48 and 78.95%) achieved in the EDFS30 [15% ethylene glycol (EG), 15% dimethyl sulfoxide (DMSO), Ficoll, and sucrose] and EFS40 [40% EG, Ficoll, and sucrose] groups were no different from those (96.15% and 83.33%) of the control group. However, the rates in the EFS30 [30% EG, Ficoll, and sucrose] (87.80 and 55.43%) and EDFS40 [20% EG, 20% DMSO, Ficoll, and sucrose] (95.69 and 70.97%) groups were significantly lower than those (96.15 and 83.33%) of the control group (P<0.05). In the experiment II, the effects of the volume of VS in the OPS on the survival of embryos after in-straw thawing were investigated. When the length of the VS in the column was less than 1 cm, the in vitro viability of embryos thawed by stepwise in-straw dilution was no different among the experimental and control groups. The embryos could be successfully thawed by immersing the OPS in 0.5 M sucrose for 3 min and then 0.25 M sucrose for 2 min. In experiment III, the effect of immersion time of the OPS in diluent (PBS) on the viability of vitrified embryos was investigated. After in-straw thawing, OPSs were immersed immediately in 1 ml PBS for 0 to 30 min. When the immersion time of the OPSs in PBS was less than 12 min, in vitro development of the in-straw thawed embryos was no different from that of the controls. In experiment IV, in-straw thawed blastocysts were directly transferred to pseudopregnant mice to examine their in vivo developmental viability. The pregnancy (91.67%) and birth rates (42.42%) of embryos in-straw thawed and directly transferred were no different from those of the unvitrified controls (90.90 and 40%) and embryos thawed by the conventional method (84.61 and 46.94%). These results demonstrate that mouse embryos vitrified with OPS could be successfully thawed by stepwise in-straw dilution and transferred directly to a recipient and that this method might be a model for field manipulation of vitrified embryos in farm animals.  相似文献   

草栖钝绥螨对二斑叶螨的捕食作用     

侯飞  邹梦婷  倪召红  乙天慈  郭建军  金道超 《植物保护学报》2023,50(2):530-537

为明确草栖钝绥螨Amblyseius herbicolus对二斑叶螨Tetranychus urticae的控制潜能,在温度分别为19、22、25、28和31℃、相对湿度均为（85±5）%、光周期均为16 L∶8 D条件下测定草栖钝绥螨对二斑叶螨各螨态的捕食偏好性、捕食功能反应及自身干扰反应。结果表明,草栖钝绥螨对二斑叶螨幼螨和第1若螨具有嗜食性,对其捕食选择系数分别为2.22和1.27,均大于1.00,对二斑叶螨卵、第2若螨和雌成螨捕食选择系数分别为0.61、0.68和0.22,均小于1.00。在不同温度条件下,草栖钝绥螨对二斑叶螨各螨态的捕食功能反应均符合HollingⅡ型;在19～31℃范围内,草栖钝绥螨对二斑叶螨各螨态的瞬时攻击率、最大日捕食量和捕食能力均随着温度升高呈先升高后降低的趋势,在28℃时达到最大值;而草栖钝绥螨对二斑叶螨各螨态的处理时间随着温度升高呈先缩短后延迟的趋势,在28℃下处理时间最短。在相同温度下,草栖钝绥螨对二斑叶螨卵、幼螨和第1若螨的捕食作用较强。在有限的捕食空间和二斑叶螨密度固定的条件下,草栖钝绥螨单头捕食量和捕食作用率随其自身密度的增加而逐渐下降,说...  相似文献   

Metabolic responses of tea (Camellia sinensis L.) to the insecticide thiamethoxam     

Weiting Jiao  Ping Zhang  Chuanjian Cui  Min Yan  Qing X. Li  Yongfeng Tang  Nan Zhang  Xinyi Wang  Ruyan Hou  Rimao Hua 《Pest management science》2023,79(10):3570-3580

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明甪直天王殿松木斗拱振动台试验研究   总被引：2，自引：0，他引：2  

阙泽利  李哲瑞  张贝贝  侯同宇  潘彪 《保鲜与加工》2015,(3):26-34

以甪直保圣寺天王殿斗拱为参考对象,进行无缩尺松木斗拱模型的地震台试验研究。通过对斗拱的加速度与动力放大系数变化趋势、斗拱在振动过程中位移响应变化特征、斗拱变形最大时刻和各构件变形最大时刻的滑移位移和回转位移数值对比分析,得出以下结论：地震加速度用于衡量地震烈度,并不能直接反映斗拱试件的最大变形;振动频率的变化对斗拱回转变形的变化起重要作用,振幅是决定各构件水平滑移的主要因素;各构件变形最大值与斗拱整体变形最大值具有很强相关性,其中栌斗和华拱的回转变形对斗拱的整体变形而言,处于支配地位;斗拱的华拱连下昂部分主要起装饰作用,其榫卯连接节点位置在振动过程中较为薄弱,在对实际文物维护修缮过程中应引起重视并采取相关加固措施。  相似文献