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61.
Hsieh YH Ofori JA Rao Q Bridgeman CR 《Journal of agricultural and food chemistry》2007,55(16):6720-6725
Bovine plasma proteins are used as high-quality protein supplements in animal feed and as binders or colorants in food for human consumption. Religious observance, as well as recent fears of epidemic bovine spongiform encephalopathy, highlights the need for methods to detect bovine blood in processed food and animal feed for regulatory purposes, as the currently available methods are neither species-specific, blood-specific, nor valid for excessively heat-processed samples. This paper reports the development of monoclonal antibodies (MAbs) raised against bovine thermostable plasma proteins that display a unique species specificity pattern for plasma proteins. Immunoblotting revealed several thermostable antigenic proteins (10, 25, 40, and 60 kDa) in bovine plasma sterilized at 121 degrees C for 15 min. These MAbs can be employed individually or combined in immunoassays for analytical purposes and investigations of the chemical and biological properties of the thermostable plasma proteins identified here. 相似文献
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Expression profiling of transgenes (Cry1Ac and Cry2A) in cotton genotypes under different genetic backgrounds 下载免费PDF全文
Kashif NOOR Hafiza Masooma Naseer CHEEMA Asif Ali KHAN Rao Sohail Ahmad KHAN 《农业科学学报》2022,21(10):2818-2832
Transgenic cotton carrying the Cry1Ac gene has revolutionized insect pest control since its adoption, although the development of resistance in insect pests has reduced its efficacy. After 10 years of cultivating Bacillus thuringiensis (Bt) cotton with a single Cry1Ac gene, growers are on the verge of adopting Bt cotton that carries the double gene (Cry1Ac+Cry2A) due to its better effectiveness against insect pests. Thus, the current study was designed to evaluate the role of each gene in the effectiveness of Bt cotton carrying the double gene. The expression levels of the Cry1Ac and Cry2A genes were evaluated in the leaves of 10 genotypes (2 parents and 8 F1 hybrids) at 30 days after sowing (DAS), while samples of leaves, bolls and flowers were taken from the upper and lower canopies at 70 and 110 DAS. The F1 hybrids were developed through reciprocal crosses between two Bt (CKC-1, CKC-2) and two non-Bt (MNH-786, FH-942) parents. The differential expression of transgenes was evaluated through Enzyme Linked Immuno-Sorbent Assay (ELISA). The results showed that the MNH786×CKC-1 hybrid had the highest concentrations of Cry1Ac gene at 30 DAS (3.08 µg g–1) and 110 DAS (1.01 µg g–1) in leaves. In contrast, the CKC-2×MNH-786 hybrid showed the lowest concentrations of Cry1Ac gene at 30 DAS (2.30 µg g–1) and 110 DAS (0.86 µg g–1). The F1 hybrid FH-942×CKC-2 showed the highest concentrations of Cry2A gene at 30 DAS (8.39 µg g–1) and 110 DAS (7.74 µg g–1) in leaves, while the CKC-1×MNH-786 hybrid expressed the lowest concentrations of Cry2A gene at 30 DAS (7.10 µg g–1) and 110 DAS (8.31 µg g–1). A comparison between the two stages of plant growth showed that leaves had the highest concentrations at 30 DAS, whereas the lowest concentrations were observed at 110 DAS for both genes in leaves. When the expression pattern was compared between various plant parts in genotype CKC-2, it was found that leaves had higher concentrations of Cry1Ac (3.12 µg g–1) and Cry2A (8.31 µg g–1) at 70 DAS, followed by bolls (Cry1Ac (1.66 µg g–1) and Cry2A (8.15 µg g–1)) and flowers (Cry1Ac (1.07 µg g–1) and Cry2A (7.99 µg g–1)). The genotype CKC-2 had higher concentrations of Cry1Ac (3.12 µg g–1) and Cry2A (8.31 µg g–1) in the upper canopy but less accumulation (2.66 µg g–1 of Cry1Ac, 8.09 µg g–1 of Cry2A) in the lower canopy at 70 DAS. Similarly, at 110 DAS, the expression levels of Cry1Ac and Cry2A in upper and lower canopy leaves were 1.52 and 7.92 µg g–1, and 0.99 and 7.54 µg g–1, respectively. Hence, the current study demonstrates that different genotypes showed variable expression for both of the Cry1Ac and Cry2A genes during plant growth due to different genetic backgrounds. The Cry2A gene had three-fold higher expression than Cry1Ac with significant differences in expression in different plant parts. The findings of this study will be helpful for breeding insect-resistant double-gene genotypes with better gene expression levels of Cry1Ac and Cry2A for sustainable cotton production worldwide. 相似文献
63.
PHANTOM 4 RTK+大疆像控处理技术在燕麦长势模拟中的应用 总被引:1,自引:1,他引:0
【目的】 利用小型消费级无人机航拍获取地物影像,通过地物阴影、高度差、色差快速提取地物,进而获取地物结构信息。【方法】 文章选取云南省曲靖市会泽县的大桥乡为研究区域,针对冬闲田闲置土地资源、种植结构相对单一的区域展开试验,利用高分辨率无人机遥感影像对燕麦进行识别,同时结合超声波传感器数据估算地物高度,并与实际高度和无人机生成的传统测高方法得到的高度进行相关性分析,获取高精度、可靠性强的数据。【结果】 基于可见光燕麦的总体分类精度为91.46%,Kappa系数为0.857,在增加DSM数据后的分类总体精度为98.91%,Kappa系数为0.982。研究表明由无人机获取的代表燕麦冠层高度信息的DSM数据能够显著提升燕麦的识别效果。相对于传统无人机测高方法生成数字表面模型提取地物高度的方法,依赖于光谱和高程信息识别地物信息的方法在计算地物高度时,精度更高,识别结果更可靠。【结论】 该文提出的小型消费级无人机利用地物阴影计算燕麦高度的方法,改进了相机镜头光心地位和RTK天线中心点地位补偿作用,打通了RTK模块、飞控模块及相机云台模块之间的通讯,能够应用于实际准确获取影像地位信息,为无人机遥感快速、准确地获取地物高度信息提供了一种新的思路。 相似文献
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本文研究了羊栖菜的有性生殖和营养繁殖。前者包括生殖托的发生、精卵的排放及合子的发育与环境因子的关系。后者主要为定株观察假根再生芽的发生。本研究为羊栖菜的人工育苗和资源保护提供了科学依据。 相似文献
66.
The effectiveness of a commercial microbial product in poorly prepared tiger shrimp, Penaeus monodon (Fabricius), ponds 总被引:5,自引:0,他引:5
The efficacy of a commercial microbial product was tested in commercial tiger shrimp, Penaeus monodon (Fabricius), ponds for one culture period in Kuala Selangor, Malaysia. Four ponds with replicates for treatment and control were used. The pond bottom was dried but the organic sludge was not removed as normally practised in pond preparation. The ponds were stocked with 15 post‐larvae at the rate of 31.m?2. Physical, chemical and biological parameters of the pond were analysed every 2 weeks during the culture period. Water quality parameters remained within the optimum range for shrimp culture except for ammonia‐nitrogen being significantly higher in control ponds and silica in treated ponds. Benthic organisms were not found in any of the ponds. The average counts of different bacteria were not significantly higher in treated ponds than control. Because of poor health, the shrimp were harvested earlier (72 days) than the usual 120 days. An average of 875.60 ± 67.00 kg shrimp ha?1 was obtained in treated ponds with a feed conversion ratio (FCR) of 1.57 ± 0.10 and survival rate of 42.35 ± 5.37% compared with 719.50 ± 130.94 kg shrimp ha?1, 2.99 ± 0.70 and 21.25 ± 3.26%, respectively, in control ponds. Neither the microbial product nor the frequent water exchange was effective in overcoming the problems caused by the poor pond bottom. 相似文献
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Liu Y. F. Liu Z. L. Rao H. Y. Wan X. Li L. H. Sun S. Y. Che M. X. Liu M. X. 《Eurasian Soil Science》2022,55(6):781-789
Eurasian Soil Science - Eight soil samples from the Jurassic Shaximiao Formation (J2s) in the Sichuan Basin, China, were taken as the research object to explore the correlation and quantitative... 相似文献
70.