Proteomic Characterization of Equine Cerebrospinal Fluid     

Carolyn J. Broccardo  Gisela Soboll Hussey  Lutz Goehring  Paul Lunn  Jessica E. Prenni 《Journal of Equine Veterinary Science》2014

Cerebrospinal fluid (CSF) is a biofluid that is reflective of overall health. Although proteomic profiling of human CSF has been performed in the context of a variety of disease states, this report represents the first comprehensive proteomic analysis of equine CSF. A total of 320 proteins were confidently identified across six healthy horses, and these proteins were further characterized by gene ontology terms mapped in UniProt, and normalized spectral abundance factors were calculated as a measure of relative abundance. Theses results provide an optimized protocol for analysis of equine CSF and lay the groundwork for future studies involving the study of equine CSF in the context of pathogenic disease states.  相似文献   

Ticks and tick borne pathogens in domestic animals in the Netherlands     

Bodaan C  Nihof AM  Postigo M  Nieuwenhuijs H  Opsteegh M  Franssen L  Jebbink F  Jansen S  Jongejan F 《Tijdschrift voor diergeneeskunde》2007,132(13):517-523

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Major genes for resistance to beet necrotic yellow vein virus (BNYVV) in Beta vulgaris     

Olga E. Scholten  Ritsert C. Jansen  L. C. Paul Keizer  Theo S. M. De Bock  Wouter Lange 《Euphytica》1996,91(3):331-339

Summary Inheritance of resistance to beet necrotic yellow vein virus (BNYVV) was studied in segregating F2 and backcross families obtained from crosses between resistant plants of the sugar beet selection Holly-1-4 or the wild beet accession Beta vulgaris subsp. maritima WB42 and susceptible parents. Greenhouse tests were carried out, in which seedlings were grown in a mixture of sand and infested soil. Virus concentrations of BNYVV in the rootlets were estimated by ELISA. To discriminate resistant and susceptible plants, mixtures of normal distributions were fitted to log₁₀ virus concentrations, estimated for segregating F1, F2 and BC populations of both accessions. The hypothesis that Holly-1-4 contained one single dominant major gene was accepted. For WB42, results fitted with the hypotheses that resistance was based on either one (or more) dominant major gene(s) showing distorted segregation, or two complementary dominant genes, which are both required for resistance. Resistance from WB42 appeared to be more effective against BNYVV than resistance from Holly-1-4.This research was carried out as part of a PhD study at the Graduate School Experimental Plant Sciences (EPS), Department of Virology, Wageningen, The Netherlands  相似文献   

Identification of tomato cultivars (Lycopersicon esculentum) by polyacrylamide isoelectric focusing     

Gisela Henn  A. W. H. Neitz  A. I. Louw 《Euphytica》1992,62(2):77-82

Summary Isozyme analyses have been used for the definitive identification of many plant cultivars, but not for cultivated tomatoes. Six isozyme systems, namely alcohol dehydrogenase, acid phosphatase, phosphoglucomutase, esterase, phosphoglucoisomerase and 6-phosphogluconate dehydrogenase of tomato seed extracts were resolved by isoelectric focusing on polyacrylamide gels with a narrow pH gradient. Nine alcohol dehydrogenase phenotypes were distinguished which, with three acid phosphatase phenotypes, identified twelve of the seventeen cultivars. Fewer differences were found for the other isozymes. Since this method could differentiate between breeding parents and their progeny it is concluded that further investigations are warranted.Abbreviations APS acid phosphatase - ADH alcohol dehydrogenase - EST esterase - IEF isoelectric focusing - PGI phosphoglucoisomerase - PGM phosphoglucomutase - 6-PGDH 6-phosphogluconate dehydrogenase - RFLP restriction fragment length polymorphism - VOPRI Vegetable and Ornamental Plant Research Institute  相似文献   

Selection for Seed Yield in Lolium perenne L.*     

Gisela Bugge 《Plant Breeding》1987,98(2):149-155

Components of seed yield were estimated for each set of 100 plants derived from six varieties of Lolium perenne. Significant differences between the varieties were found for all components of seed yield, but the range was broader within each variety. An indirect selection of seed yield using the character seeds per spike produced a selection response similar to that obtained by direct selection on high seed yield per plant. A direct and indirect selection of characters estimated in small, drilled plots of topcross off-springs (F₁) was less successful than the selection based on the single plants. Spearman rank correlation coefficients between the single plants and their offsprings did not show significant agreement for seed yield. But altogether the results indicated that the increase of seed yield/area was about 6 % when 10 % of the best plants were selected.  相似文献   

Interactions between plant and aphid genotypes in resistance of lettuce to Myzus persicae and Macrosiphum euphorbiae     

K. Reinink  F. L. Dieleman  J. Jansen  A. M. Montenarie 《Euphytica》1989,43(3):215-222

Summary Six lettuce lines, representing two types of resistance to the green peach aphid, Myzus persicae, and a control line with high susceptibility to M. persicae were tested for resistance to six different clones of Myzus persicae and two clones of the potato aphid, Macrosiphum euphorbiae.The clones of M. persicae showed very different levels of aggressiveness on lettuce: two had a high level of reproduction, two had an intermediate level and two were poorly adapted to lettuce as a host. Differences between lettuce lines in aphid reproduction increased with increasing aggressiveness of the aphid clone, which means that aggressive clones are most effective for selection purposes. No evidence was found for clone-specific plant genotype reactions, meaning that lines resistant to one clone will also be resistant to other clones of M. persicae, allthough not neccessarily at the same level. The lettuce lines selected for partial resistance to the aggressive clone WMp1 were completely or almost completely resistant to less aggresive clones.No differences in level of reproduction were found between the two clones of M. euphorbiae and no relation was observed between resistance to M. persicae and M. euphorbiae, indicating the species-specific character of resistance to leaf aphids in lettuce.  相似文献   

Mikrosymptome des Bor-Mangels an Mentha piperita L.     

Gisela Fischer  W. Bussler 《植物养料与土壤学杂志》1984,147(1):13-20

Microsymptoms of boron deficiency in Mentha piperita L. The influence of boron deficiency on the development of oil glands and glandular hairs and on leaf and stem tissue development was studied. The experiments were carried out with Mentha piperita L. – var. Mitcham – under controlled experimental conditions in solution culture. Boron deficiency resulted in disturbances in the development of the vascular bundles and cambium extension. Excretions of dark substances could also be observed. Oil glands as well as glandular hairs were severely damaged under boron deficiency; the production of the essential oil decreased.  相似文献   

Establishment of Retama sphaerocarpa L. seedlings on a degraded semiarid soil as influenced by mycorrhizal inoculation and sewage‐sludge amendment     

María del Mar Alguacil  Fuensanta Caravaca  Gisela Díaz  Purificacin Marín  Antonio Roldn 《植物养料与土壤学杂志》2004,167(5):637-644

A field experiment was carried out to evaluate the effectiveness of mycorrhizal inoculation with three arbuscular mycorrhizal (AM) fungi (Glomus intraradices Schenck & Smith, Glomus deserticola (Trappe, Bloss. & Menge), and Glomus mosseae (Nicol & Gerd.) Gerd. & Trappe) and the addition of composted sewage sludge (SS) with respect to the establishment of Retama sphaerocarpa L. seedlings, in a semiarid Mediterranean area. Associated changes in soil chemical (nutrient content and labile carbon fractions), biochemical (enzyme activities), and physical (aggregate stability) parameters were observed. Six months after planting, both the addition of composted SS and the mycorrhizal‐inoculation treatments had increased total N content, available‐P content, and aggregate stability of the soil. Values of water‐soluble C and water‐soluble carbohydrates were increased only in the mycorrhizal‐inoculation treatments. Rhizosphere soil from the mycorrhizal‐inoculation treatments had significantly higher enzyme activities (dehydrogenase, protease‐BAA, acid phosphatase, and β‐glucosidase) than the control soil. In the short‐term, mycorrhizal inoculation with AM fungi was the most effective treatment for enhancement of shoot biomass, particularly with G. mosseae (about 146% higher with respect to control plants). The addition of the composted SS alone was sufficient to restore soil structural stability but was not effective with respect to improving the performance of R. sphaerocarpa plants.  相似文献   

Dorsal spinocerebellar tract: response pattern of nerve fibers to muscle stretch   总被引：1，自引：0，他引：1  

J K Jansen  T Rudjord 《Science (New York, N.Y.)》1965,149(688):1109-1111

The response to muscle stretch of second-order neurons of the dorsal spinocerebellar tract was studied in anesthetized cats. Three different types of neurons are defined; the response of each type is remarkably similar to that of the corresponding stretch-receptors of the muscle. Accordingly, the cerebellum receives information on the mechanical situation in muscle from three qualitatively different channels: one has a high degree of sensitivity to dynamic stretch (Ia units) and provides a mixed signal of muscle length and speed of movement; another provides information mainly on muscle length (group-Il units); and the third signals information on the degree of contraction of the muscle (Ib units).  相似文献   

Mutations in dynein link motor neuron degeneration to defects in retrograde transport     

Hafezparast M  Klocke R  Ruhrberg C  Marquardt A  Ahmad-Annuar A  Bowen S  Lalli G  Witherden AS  Hummerich H  Nicholson S  Morgan PJ  Oozageer R  Priestley JV  Averill S  King VR  Ball S  Peters J  Toda T  Yamamoto A  Hiraoka Y  Augustin M  Korthaus D  Wattler S  Wabnitz P  Dickneite C  Lampel S  Boehme F  Peraus G  Popp A  Rudelius M  Schlegel J  Fuchs H  Hrabe de Angelis M  Schiavo G  Shima DT  Russ AP  Stumm G  Martin JE  Fisher EM 《Science (New York, N.Y.)》2003,300(5620):808-812

Degenerative disorders of motor neurons include a range of progressive fatal diseases such as amyotrophic lateral sclerosis (ALS), spinal-bulbar muscular atrophy (SBMA), and spinal muscular atrophy (SMA). Although the causative genetic alterations are known for some cases, the molecular basis of many SMA and SBMA-like syndromes and most ALS cases is unknown. Here we show that missense point mutations in the cytoplasmic dynein heavy chain result in progressive motor neuron degeneration in heterozygous mice, and in homozygotes this is accompanied by the formation of Lewy-like inclusion bodies, thus resembling key features of human pathology. These mutations exclusively perturb neuron-specific functions of dynein.  相似文献