覆盖对红叶石楠和红豆杉生长的影响     

张金莎  王平  陈永安  舒东膂  胡勇 《湖南林业科技》2014,(5):63-64

在相同地域条件下,采用传统养护方式与覆盖养护方式的对比试验,研究覆盖对红叶石楠和红豆杉生长的影响。结果表明:合理有效的覆盖养护方式,可以明显的改良土壤肥力,促进植株生长。  相似文献   

Screening for reproductive biomarkers in Bactrian camel via iTRAQ analysis of proteomes     

Qi Wang  Quanwei Zhang  Ze Gan  Haijiang Li  Yang Yang  Yong Zhang  Xingxu Zhao 《Reproduction in domestic animals》2020,55(2):189-199

Bactrian camel is an ancient and precious species of livestock; that is, unique resources exist in the desert and have important economic and scientific value. In recent years, the number of Bactrian camels has declined sharply. Due to its long reproductive cycle and seasonal oestrus, the mechanism of oestrus is unknown. To identify candidate biomarkers of reproduction, we performed a comprehensive proteomic analysis of serum from Bactrian camel in oestrus and non-oestrus, using isobaric tags for relative and absolute quantitation (iTRAQ) coupled with tandem mass spectrometry. We identified 359 proteins, of which 32 were differentially expressed: 11 were up-regulated and 21 were down-regulated in samples from camels in oestrus. We validated the differential expression of a subset of these proteins using qPCR and Western blot. Gene ontology annotation identified that the differentially expressed proteins function in cellular processes, metabolic processes and immune system processes. Notably, five of the differentially expressed proteins, PCGF5, histone H1.2, RBP4, FOLR1 and ANTXR2, are involved in reproductive regulatory processes in other animals. KEGG enrichment analysis demonstrated significant enrichment in several cardiac-related pathways, such as ‘dilated cardiomyopathy’, ‘hypertrophic cardiomyopathy’, ‘cardiac muscle contraction’ and ‘adrenergic signalling in cardiomyopathy’. Our results suggest that candidate biomarker (PCGF5, histone H1.2, RBP4, FOLR1 and ANTXR2) discovery can aid in understanding reproduction in Bactrian camels. We conclude that the profiling of serum proteomes, followed by the measurement of selected proteins using more targeted methods, offers a promising approach for studying mechanisms of oestrus.  相似文献   

The priority of management factors for reducing the yield gap of summer maize in the north of Huang-Huai-Hai region,China          下载免费PDF全文

Yue-e LIU  Yu-xin LI  Tian-fang LÜ  Jin-feng XING  Tian-jun XU  Wan-tao CAI  Yong ZHANG  Jiu-ran ZHAO  Rong-huan WANG 《农业科学学报》2021,20(2):450-459

Understanding yield potential, yield gap and the priority of management factors for reducing the yield gap in current intensive maize production is essential for meeting future food demand with the limited resources. In this study, we conducted field experiments using different planting modes, which were basic productivity(CK), farmer practice(FP), high yield and high efficiency(HH), and super high yield(SH), to estimate the yield gap. Different factorial experiments(fertilizer, planting density, hybrids, and irrigation) were also conducted to evaluate the priority of individual management factors for reducing the yield gap between the different planting modes. We found significant differences between the maize yields of different planting modes. The treatments of CK, FP, HH, and SH achieved 54.26, 58.76, 65.77, and 71.99% of the yield potential, respectively. The yield gaps between three pairs: CK and FP, FP and HH, and HH and SH, were 0.76, 1.23 and 0.85 t ha~(–1), respectively. By further analyzing the priority of management factors for reducing the yield gap between FP and HH, as well as HH and SH, we found that the priorities of the management factors(contribution rates) were plant density(13.29%)fertilizer(11.95%)hybrids(8.19%)irrigation(4%) for FP to HH, and hybrids(8.94%)plant density(4.84%)fertilizer(1.91%) for HH to SH. Therefore, increasing the planting density of FP was the key factor for decreasing the yield gap between FP and HH, while choosing hybrids with density and lodging tolerance was the key factor for decreasing the yield gap between HH and SH.  相似文献   

夏训对优秀皮划艇运动员铁调素等铁营养状况以及IL-6的影响     

王力先    赵君军  李捷    廖红娟  丘〓卫  季健民  张〓援 《上海交通大学学报(农业科学版)》2014,(5):102-104

目的：探讨夏训对优秀运动员铁调素等铁营养状况以及IL-6的影响。方法：广东省优秀皮划艇运动员26人(男15人，女11人)参加测试，调查夏训前和夏训后血红蛋白、铁调素、可溶性转铁蛋白受体、血清铁蛋白、可溶性转铁蛋白受体/log铁蛋白、血清铁、总铁结合力、转铁蛋白饱和度以及白介素6变化情况。结果：夏训后，男运动员组铁调素和可溶性转铁蛋白受体极显著上升(P<0.01)、血清铁蛋白极显著下降(P<0.01)，可溶性转铁蛋白受体/log铁蛋白显著上升(P<0.05)，总铁结合力显著下降(P<0.05)，白介素6极显著上升(P<0.01)。女运动员组血红蛋白显著降低(P<0.05)，铁调素和可溶性转铁蛋白受体极显著上升(P<0.01)，血清铁蛋白极显著下降(P<0.01)，可溶性转铁蛋白受体/log铁蛋白极显著上升(P<0.01)，白介素6极显著上升(P<0.01)。结论：夏训可引起运动员炎症因子升高、铁调素升高，导致功能铁不足；铁调素介导的铁代谢紊乱可能是运动性低血色素发生的重要原因。  相似文献   

超学科知识生产模式对农村地区多媒体英语教学的启示     

王永 《农业网络信息》2014,(2):125-127

随着知识经济的兴起,人类传统的知识生产模式正悄然发生革命性的变化。一种被称之为"超学科"的知识生产模式开始崭露头角,它强调知识生产要在多学科共同参与的广阔应用语境下进行,要在各利益方充分商榷、谈判、让步、协调、合作的基础上,形成最终共识,从而找到解决问题的途径。和传统的知识生产模式相比,超学科知识生产模式具有应用语境性、超学科性、异质性、反思性和质量控制标准多元性等典型特征,因而可以对农村地区多媒体英语教学具有良好的启示作用。  相似文献   

拮抗农业致病菌放线菌G5发酵条件的优化     

刘杨  王勇  康静芹  张良 《湖北农业科学》2014,(7):1634-1636,1689

微生物的发酵过程十分复杂,培养基配方的组成以及培养条件的变化都会对菌体的代谢造成很大的影响。为了使抗生素的产量达到最大化,试验运用了Plackett-Burman试验设计、最陡爬坡试验、BoxBehnken试验设计对放线菌(Actinomycetes)G5进行了发酵条件优化。利用Plackett-Burman试验设计对影响放线菌G5发酵的诸多相关因素进行评估并筛选出3个主要因素,即碳源含量、培养温度和培养时间;之后根据3个主要因素的作用大小和方向进行了最陡爬坡试验;最后运用Box-Behnken试验设计确定最优值,得出其最优培养条件为淀粉含量21.66 g/L、培养温度27.70℃、培养时间7.75 d。在此条件下,抗生素对苹果轮纹病菌的抑菌圈直径为23.00 mm,与模型预测值基本一致。  相似文献   

Development of Nucleic Acid Lateral Flow Immunoassay Detection Method for Foot-and-mouth Disease Virus     

GONG Zhen-li  JIANG Tao  QI Shu-yun  CHEN Guo-dong  LIU Yan-hong  LI Yong 《中国畜牧兽医》2015,42(9):2292-2297

In this article, through the combination of nucleic acid probes and immune chromatography, a simple, sensitive and specific detection system——nucleic acid lateral flow immunoassay (NALFIA) for amplifing foot-and-mouth disease virus (FMDV) 3D RT-PCR products was established.An ultrasensitive nucleic acid biosensor (NAB) based on streptavidin-labeled gold nanoparticles dual labels and lateral flow strip biosensor (LFSB) were used in this system.The biotinylated goat anti-rabbit IgG was marked to the NC membrane as the alleged strip and the anti-digoxin antibody was labeled to the NC membrane to capture the digoxin probe.After assemblying gold-labeled strip and detecting RT-PCR products, the detection limit of NALFIA was 0.3×10^-3 to 3×10^-3 μg/μL.The NALFIA was compared with agar gel electrophoresis analysis, the results showed that the sensitivity of NALFIA was higher than agar gel electrophoresis.There was an excellent agreement between the two methods.NALFIA was a method with high sensitive, low cost and short time.In conclusion, this method provided a good alternative to detect FMDV.  相似文献   

酸性土壤中决明属牧草高效根瘤菌的分离及筛选     

杨庆  王义祥  李欣欣  翁伯琦  廖红 《草地学报》2020,28(6):1519-1526

为提高豆科决明属（Chamaecrista spp.）牧草的结瘤能力和固氮效率，促进决明属牧草在酸性土壤中的应用及推广，本研究以圆叶决明（Chamaecrista rotundifolia）和羽叶决明（Chamaecrasta nictitans）为试验材料，通过分离、纯化、nodA基因鉴定等方法，从酸性土壤里决明属牧草的根瘤中分离到10株候选根瘤菌株系。16S rDNA测序及系统发育树分析表明，这10株根瘤菌均属于慢生型根瘤菌。回接试验表明，上述10株根瘤菌均能与圆叶决明共生，形成根瘤。与不接种的对照相比，其固氮酶活性为0.08~4.65 μmol·g^-1·h^-1，植株干重、SPAD值和氮含量分别提高了99.17%~372.14%，399.27%~789.05%，235.00%~1043.33%。其中，4株根瘤菌TXR2，TXN1，WYSR1，JYN6对圆叶决明氮营养贡献最大，并且TXN1与宿主共生形成的根瘤数目最多，而JYN6的固氮酶活性最高。说明本研究分离鉴定到4株决明高效根瘤菌，具有较好的应用前景。  相似文献   

牡丹5个管家基因的克隆及其在系统进化分析中的应用   总被引：1，自引：0，他引：1  

杨勇  王顺利  薛璟祺  任秀霞  李丹丹  杨若雯  曾秀丽  张秀新 《园艺学报》2016,43(2):307-313

利用RT-PCR技术从牡丹组7个野生种和1个栽培种中分别克隆得到泛素延伸蛋白基因(ubiquitin,UBI)、素环蛋白基因(cyclophilin,CYP)、肌动蛋白基因(Actin)、葡萄糖六磷酸脱氢酶基因(glucose-6-phosphate 1-dehydrogenase,G6PD)和β–微管蛋白基因(beta-tubulin,TUB)等5个管家基因的编码序列(coding sequence,CDS)。序列比对分析发现,5个管家基因序列均相对保守,其在牡丹7个野生种和1个栽培种中的保守性在99.04%~99.69%。利用5个管家基因分别构建了其在牡丹组8份材料中的系统进化树。5个系统进化树均将8份材料分成两组,即革质花盘亚组和肉质花盘亚组;由于5个管家基因核苷酸序列各自的保守性不同,在区分亚组成员间略有差异。综合分析5个管家基因构建的系统进化树可知,杨山牡丹(Paeonia ostii)和栽培牡丹(P.suffruticosa‘Luoyanghong’)常聚为一支,二者亲缘关系较近,因而推测杨山牡丹可能参与了栽培牡丹的形成;紫斑牡丹(P.rockii)和四川牡丹(P.decomposita)一直聚为一支,推测二者亲缘关系较近;黄牡丹(P.lutea)和紫牡丹(P.delavayi)一直聚为一支,推测这二者亲缘关系较近。狭叶牡丹(P.potaninii)与黄牡丹和紫牡丹分化较早,结合前人研究结果建议将狭叶牡丹作为一个独立的种。  相似文献   

Analysis on Target Genes with Mutation in microRNA of Blue-shelled Chicken and White Leghorn     

GUAN Yu-yu  LIAO Rong-rong  WANG Zhen  ZHAO Jing  CHEN Zhen-liang  ZHANG Zhe  XIAO Qian  SUN Hao  WANG Qi-shan  ZHANG Xiang-zhe  YANG Chang-suo  PAN Yu-chun 《中国畜牧兽医》2016,43(6):1413-1421

The study was conducted to explore the potential different characters between Blue-shelled chicken and White leghorn.Global genome microRNA was combined the identified microRNA with complementary lab-predicted microRNA.Then the two breed chicken's SNP data got by GGRS were mapped to the microRNA and focused on SNP that deliberately located in mature-microRNA.Bioinformatics method was adopted for target prediction on microRNA which had SNPs.By further gene enrichment analysis,the study found these genes enriched in 22 GO terms,10 KEGG pathways,and 3 IPA important networks.And they enriched in traits which associated with growth,such as mTOR signaling pathways,Wnt signaling pathways,growth hormone receptor networks and insulin-like growth factor Ⅰ receptor networks.And they also enriched in some laying traits,such as oocyte meiotic signaling pathways and progesterone mature oocytes signaling pathways.The methods and the results might provide references for further studies.  相似文献