圆斑星鲽MHC IIB基因结构、多态性及组织表达分析   总被引：1，自引：1，他引：0  

李宏俊  王娟  高祥刚  苏浩  刑坤  傅立元  赫崇波 《中国水产科学》2011,18(2):299-307

通过表达序列标签法和cDNA末端快速扩增(RACE)技术,分离和克隆了圆斑星鲽(Verasper variegatus)主要组织相容性复合体(MHC)IIB的全长cDNA序列,该cDNA全长为1144bp,5'UTR(untranslated region)为7bp,3'UTR为450bp,开放阅读框(ORF)长度为687bp,可编码228个氨基酸,包含信号肽、抗原结合域(β1)、IGC区(β2)、跨膜区和胞质区5个结构域。同源分析表明,圆斑星鲽MHCIIB氨基酸序列与其他硬骨鱼具有49%～79%的同源性,与鼠、人、红原鸡和护士鲨的相似性较低,分别为34%、33%、31%和30%。圆斑星鲽MHC ⅡB基因含有5个内含子,与其他硬骨鱼不同,其β2结构域编码区内存在1个109bp的内含子。根据获得的MHC ⅡB基因组序列设计特异性引物,在10尾野生圆斑星鲽中扩增了包括完整内含子1和外显子2的长度约388bp的DNA片段,PCR产物直接测序后发现在270bp的抗原结合域中共有23个位点发生变异,密码子第1位和第2位的变异明显高于第3位。利用荧光定量PCR分析组织表达发现,MHCIIB基因在健康圆斑星鲽9种组织中均有...  相似文献   

Relationships between total adiponectin concentrations and obesity in native-breed ponies in England     

Marine A. Barnabé  Jonathan Elliott  Patricia A. Harris  Nicola J. Menzies-Gow 《Equine veterinary journal》2024,56(2):264-272

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Screening for pollen tolerance to high temperatures in tomato     

Marine J. Paupière  Pauline van Haperen  Ivo Rieu  Richard G. F. Visser  Yury M. Tikunov  Arnaud G. Bovy 《Euphytica》2017,213(6):130

Among the abiotic stresses affecting plant reproduction, high temperature is one of the most prominent ones because it directly affects fruit set. So far, little attention has been paid to the investigation of the variation in high temperature tolerance among wild tomato (Solanum lycopersicum) germplasm. The objective of this study was to determine the tolerance of 17 different cultivated and wild tomato accessions to high temperature, using a pollen viability screening approach. Each of the 17 genotypes of tomato was analysed for their pollen quality under a 32 °C (day)/26 °C (night) regime. The total number of pollen per flower and the fraction of viable pollen were recorded. The number of pollen per flower varied between 35,547 and 109,490 whereas the fraction of viable pollen varied between 0.03 and 0.71. No correlation was found between these two traits. However, the combination of these traits could provide the best reproductive capability under high temperature. In this study, thermo-tolerant (LA2854, LA1478 and LA0417) as well as thermo-sensitive (LA1719, LA1580, and SWEET4) genotypes have been identified. Those genotypes can be used as novel genetic resources to get more insight into pollen thermo-tolerance mechanisms and be included in breeding programs.  相似文献   

Acknowledgement to Reviewers of Marine Drugs in 2014     

Marine Drugs Editorial Office 《Marine drugs》2015,13(1):267-275

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Acknowledgement to Reviewers of Marine Drugs in 2015     

Marine Drugs Editorial Office 《Marine drugs》2016,14(1)

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Cambrian river terraces and ridgetops in central australia: oldest persisting landforms?     

Stewart AJ  Blake DH  Ollier CD 《Science (New York, N.Y.)》1986,233(4765):758-761

Fluvial sediments in paleovaileys cut in the Ashburton surface of the Davenport province of central Australia form terrace remnants that appear to retain their original depositional tops and have probably existed as subaerial landforms since their inception. Marine fossils in sediments conformable with the fluvial sediments near the southeast margin of the province give a Cambrian age for the terraces; the Ashburton surface forming the ridgetops between the paleovalleys is Cambrian or older.  相似文献   

Biological investigations of anchovy and some selected fish species caught during the R／V “Bei Dou”surveys 1984—1999     

ArneJohannessen  金星仕 《海洋水产研究》2001,22(4):46-56

IntroductionProductivity of an ecosystem may be measured in terms of structure,abundance and growth of keyspecies.Stock structure,measured as age or size composition in a given stock may indicate whether the stockis in a state of growing,stable or declining.Individual growth may be measured in terms of annual growth ofindividual age groups and maturity of key species will similarly reflect the potential of the species to copewith the influence of a changing environment of predators and human i…  相似文献   

江豚的形态特征和亚种划分问题   总被引：12，自引：0，他引：12  

王丕烈 《水产科学》1992,11(11):4-8

本文通过对各海域江豚主要形态特征性状差异显著比较,讨论了江豚亚种划分问题。作者认为江豚包含3个亚种:南海种群与印度,巴基斯坦种群同属指名亚种Neophocaena phocaenoides phocaenoides,东海一长江种群属杨子亚种N.p.asiaeorientalis,黄渤海种群与日本种群均属北方亚种N.p.sunameri。  相似文献   

Dispersal-based species pools as sources of connectivity area mismatches     

Préau  Clémentine  Dubos  Nicolas  Lenormand  Maxime  Denelle  Pierre  Le Louarn  Marine  Alleaume  Samuel  Luque  Sandra 《Landscape Ecology》2022,37(3):729-743

Landscape Ecology - Prioritising is likely to differ depending on the species considered for connectivity assessments, leading to a lack of consensual decisions for territorial planning. The...  相似文献   

Enhancing septoria leaf blotch forecasts in winter wheat I: the effect of temperature on the temporal distance between critical rainfall periods and the breaking of the control threshold     

Beyer  Marco  Pallez-Barthel  Marine  Dam  Doriane  Hoffmann  Lucien  El Jarroudi  Moussa 《植物病害和植物保护杂志》2022,129(1):37-44

Journal of Plant Diseases and Protection - In integrated pest management (IPM), pests are controlled when the costs of control correspond with the damage caused by a pest on a monetary scale,...  相似文献