Effects of porcine reproductive and respiratory syndrome virus (isolate tw91) on porcine alveolar macrophages in vitro     

Chiou MT  Jeng CR  Chueh LL  Cheng CH  Pang VF 《Veterinary microbiology》2000,71(1-2):9-25

To verify the role of porcine reproductive and respiratory syndrome virus (PRRSV) infection on pulmonary defense mechanisms, alterations in the viability, morphology, and various functions of porcine alveolar macrophages (AMs) were evaluated in vitro for 2-72 h after exposure to a Taiwan isolate, tw91, at a multiplicity of infection (MOI) of 0.1. A low but constant rate of infection, around 5%, was seen in AMs from the PRRSV-infected group throughout the study. When compared with a mock-infected group, AMs from the PRRSV-infected group had a significantly lower viability at 18-72 h post-infection (HPI) as determined by trypan blue dye exclusion. Also during this time period, the cells showed morphological changes, including rounding, bleb formation, and rupture. The phagocytic and microbicidal capacity of AMs against Candida albicans was significantly inhibited after 6 HPI. Although the total amount of superoxide anion (O2-) and hydrogen peroxide (H2O2) produced by the AMs was reduced after 18 and 12 HPI, respectively, the amount of production was enhanced in both reactive oxygen species on a per viable cell basis after 12 HPI. In contrast, the level of bioactive tumor necrosis factor alpha (TNF-alpha) secretion, either total or on a per viable cell basis, was markedly reduced soon after PRRSV infection, up to 36 HPI, followed by a rebound thereafter. Prostaglandin E2 (PGE2) production was enhanced, both in total and on a per viable cell basis, in the first 6 h of infection, especially at 2 HPI. However, it became lower than that of the control after 36 HPI. The results indicated that PRRSV infection could cause, directly and/or indirectly, not only death of AMs but also adverse alterations in their morphology and function, although some of the effects seemed to be reversible. Because AMs are crucial to the host against airborne pathogens, PRRSV infection may potentially predispose pigs to secondary pulmonary infections.  相似文献   

河南警犬参与侦破命案4例     

庞辉  徐奎 《中国工作犬业》2007,(4):8-8

2007年1月26日,河南省郸城县东风乡发生一起入室抢劫杀人案,受害人侯某被人杀死在自家床上,家中被抢走现金9700元。接指令后,淮阳县公安局带犬民警徐奎携警犬“追命”赶赴现场。在带犬民警的指挥引导下,  相似文献   

Partial pressure of end-tidal CO₂ sampled via an intranasal catheter as a substitute for partial pressure of arterial CO₂ in dogs     

D. Pang  BVSc    J. Hethey  N. A. Caulkett  DVM  MVetSc  DACVA    T. Duke  BVetMed  DVA  DACVA  DECVA 《Journal of Veterinary Emergency and Critical Care》2007,17(2):143-148

Objective: To demonstrate correlation and clinical usefulness of the partial pressure of end‐tidal CO₂ (ETCO₂) measurement by nasal catheter placement in sedated dogs with and without concurrent nasal oxygen administration as a substitute for partial pressure of arterial CO₂ (PaCO₂). Design: Prospective, cross‐over trial. Setting: University of Saskatchewan veterinary research laboratory. Animals: Six cross‐breed dogs with a mean (±SD) weight of 29.1±4.03 kg. Interventions: All dogs were sedated with 5 μg/kg medetomidine intravenously (IV) and an arterial catheter was placed in a dorsal pedal artery for removal of blood for gas analysis. A nasal catheter was placed in the ventral meatus and connected to a capnometer for ETCO₂ measurements in all dogs. Dogs receiving supplemental nasal oxygen had a second nasal catheter placed in the contralateral naris. Measurements and main results: In the group without nasal oxygen supplementation, the ETCO₂ measurement underestimated (negative bias) the PaCO₂ by ?2.20 mmHg with limits of agreement (95% confidence interval) of ?5.79, 1.39 mmHg. In the group receiving oxygen supplementation, ETCO₂ measurement underestimated (negative bias) the PaCO₂ by ?2.46 mmHg with limits of agreement (95% confidence interval) of ?8.42, 3.50 mmHg. Conclusions: The results of this study demonstrate that ETCO₂ monitoring via a nasal catheter provides a clinically acceptable substitute to arterial blood gas analysis as a means of monitoring ventilation in healthy, sedated dogs. The limits of agreement were within acceptable limits with and without concurrent insufflation of oxygen.  相似文献   

警犬识破暴雨中的凶杀罪恶     

庞辉  徐奎 《中国工作犬业》2007,(8):13-14

2007年7月14日下午17时许,河南省淮阳县冯塘乡罗庄村村民宋某之女在本村附近失踪,其家人及亲戚在多方寻找无果的情况下,于当晚20时报案. 　　接到报警后,冯塘派出所民警连夜赶往失踪者家中进行调查了解情况.并协助其家人在暴雨中积极寻找失踪者,但一夜无果.7月15日上午8时许,失踪女宋某的尸体在该村西北角树林西边的水沟内被发现,派出所民警随即将这一情况向淮阳县公安局领导及刑警大队报告.局长段业林当即指令全局启动命案侦破预案,同时调动警犬到现场协助破案.……  相似文献   

成功治愈犬瘟热2例     

庞辉 《中国工作犬业》2006,(10):26-27

犬瘟热是由犬瘟热病毒(CDV)引起的,是感染肉食兽中的犬科(尤其是幼犬),鼬科及一部分浣熊科动物的高度接触性、致死性传染病。病犬早期表现双相热型、急性鼻卡他,随后以支气管炎、卡他性肺炎、严重胃肠炎和神经症状为特征,少数病例出现鼻端和脚垫的高度角化。笔者在临床工作中,遇到过多例犬瘟热病例但仅有此2例成功治愈,现介绍如下:一、基本情况2006年4月18日晨,某犬场饲养员发现5头犬有咳嗽、厌食、流浓鼻涕等症状。兽医经过诊断,确诊其中一头藏獒(公,3月龄,10千克体重)为犬瘟热。4月19日晨,又有一头血犬(公,14月龄,35千克左右)发病:咳嗽…  相似文献   

魔芋葡甘聚糖在环保、医药及食品中的应用   总被引：7，自引：0，他引：7  

彭述辉  庞杰 《长江蔬菜》2006,(12):30-33

对魔芋葡甘聚糖的特性及在环保、医药、食品及其他领域中的广阔应用进行了综述,讨论了魔芋葡甘聚糖的环保性能、药理作用、食品加工等方面的应用前景.  相似文献   

木本婆婆纳‘维力镜像’的组培快繁技术     

蒋亚莲  熊丽  吴丽芳  屈云慧  陶磅 《园艺学报》2006,33(3):656-656

木本婆婆纳（Hebe）原产澳洲,主要分布于新西兰和澳大利亚的东南部,全属约100种,栽培品种超过800个,是一类观赏常绿花灌木.在澳洲和欧洲,被广泛应用在观赏园艺和庭院绿化上,并对Hebe属植物的育种和栽培做了大量的研究.近年来,我国也开始逐步认识和引种Hebe属植物,并有少量用于生产,但组培方面的研究国内尚未见报道.  相似文献   

添加有机硅表面活性剂对低容量喷雾防治小麦蚜虫的影响   总被引：11，自引：2，他引：11  

邱占奎  ;袁会珠;李永平;纪明山;黄雄英;逄森 《植物保护》2006,32(2):34-37

室内试验表明,有机硅表面活性剂Silwet408、非离子表面活性剂OP-10和JFC可显著降低药液的表面张力,其中Silwet408在质量分数为0.05%时可使水的表面张力降到22.05 mN/m,表面活性最强。当药液中含有0.001%的有机硅表面活性剂Silwet408时,其在小麦叶片上的接触角即开始显著减小,且在小麦背面的接触角小于在正面的接触角。在10%吡虫啉可湿性粉剂稀释2500倍药液中添加Silwet408,药液的表面张力和其在小麦叶片上的接触角明显降低,雾滴在小麦叶片上扩展面积明显增大。进一步田间试验表明,使用手持离心式低容量喷雾机喷雾,在10%吡虫啉可湿性粉剂药液中添加质量分数为0.05%和0.1%的Silwet408,可显著增加对小麦蚜虫的防治效果。  相似文献   

杆状病毒非必需基因的结构与功能   总被引：1，自引：0，他引：1  

李充璧  闫庆生 《昆虫天敌》1998,20(3):118-123

本文主要以苜蓿丫纹夜蛾核型多角体病毒（ＡｃＭＮＰＶ）为例,概述了迄今展报道的杆状病毒非必需基因的结构与功能,为杆状病毒的基因工程研究提供参考。  相似文献   

苏云金杆菌杀虫晶体蛋白作用机制的研究进展   总被引：1，自引：0，他引：1  

余健秀  余榕捷 《昆虫天敌》1998,20(4):180-186

自本世纪６０年代以来,由于化学农药引起的环境污染、害虫普遍产生抗药性等原因,生物防治越来越受到人们的重视。在现有的各种生物防治策略中,研究和利用微生物杀虫剂发展很快,最突出的方法是利用苏云金杆菌（Ｂａｃｉｌｕｓｔｈｕｒｉｎｇｉｅｎｓｉｓ,Ｂｔ）进行防...  相似文献