全文获取类型
| 收费全文 | 40896篇 |
| 免费 | 1297篇 |
| 国内免费 | 2782篇 |
专业分类
| 林业 | 6579篇 |
| 农学 | 5112篇 |
| 基础科学 | 2201篇 |
| 6906篇 | |
| 综合类 | 8128篇 |
| 农作物 | 3555篇 |
| 水产渔业 | 2814篇 |
| 畜牧兽医 | 4183篇 |
| 园艺 | 2016篇 |
| 植物保护 | 3481篇 |
出版年
| 2024年 | 70篇 |
| 2023年 | 301篇 |
| 2022年 | 703篇 |
| 2021年 | 995篇 |
| 2020年 | 899篇 |
| 2019年 | 893篇 |
| 2018年 | 3274篇 |
| 2017年 | 3538篇 |
| 2016年 | 1939篇 |
| 2015年 | 1118篇 |
| 2014年 | 1119篇 |
| 2013年 | 1382篇 |
| 2012年 | 2359篇 |
| 2011年 | 3750篇 |
| 2010年 | 3751篇 |
| 2009年 | 2748篇 |
| 2008年 | 2700篇 |
| 2007年 | 2899篇 |
| 2006年 | 1423篇 |
| 2005年 | 1328篇 |
| 2004年 | 676篇 |
| 2003年 | 657篇 |
| 2002年 | 526篇 |
| 2001年 | 452篇 |
| 2000年 | 579篇 |
| 1999年 | 636篇 |
| 1998年 | 611篇 |
| 1997年 | 502篇 |
| 1996年 | 431篇 |
| 1995年 | 424篇 |
| 1994年 | 416篇 |
| 1993年 | 392篇 |
| 1992年 | 319篇 |
| 1991年 | 264篇 |
| 1990年 | 230篇 |
| 1989年 | 191篇 |
| 1988年 | 164篇 |
| 1987年 | 98篇 |
| 1986年 | 58篇 |
| 1985年 | 24篇 |
| 1984年 | 19篇 |
| 1983年 | 28篇 |
| 1982年 | 24篇 |
| 1981年 | 27篇 |
| 1980年 | 19篇 |
| 1979年 | 4篇 |
| 1977年 | 4篇 |
| 1968年 | 4篇 |
| 1963年 | 1篇 |
| 1956年 | 1篇 |
排序方式： 共有10000条查询结果，搜索用时 15 毫秒
991.
Context
The assessment of land-use impacts on biodiversity is one of the central themes of landscape ecology and conservation biology. However, due to the complexity of biodiversity, it is impossible to obtain complete information about the diversity of all species even for small areas, necessitating the selection of individual species or assemblages thereof as species surrogate. In parts of the world where taxonomic expertise is lacking, species identification has hindered progress in biodiversity conservation, and the only practical, relatively-accurate option, is the use of taxonomic minimalism.Objective
We carried out a rapid biodiversity assessment based on three surrogates—land-use (driver-surrogate), terrestrial arthropods (species-surrogate) and morphospecies (taxonomic-surrogate)—to determine the impacts of land-use on biodiversity of the Western Region (Ghana), an area covering ~4 % of the West African biodiversity hotspot.Method
We used diversity profiles to visualize the distribution of a total of 8848 arthropod individuals over seven land-use types which define the complete heterogeneity of the landscape.Results
Here, we present both sample and asymptotic diversity profiles of arthropod morphospecies for each land-use type and the potential of each land-use type for conserving arthropods.Conclusions
We conclude that (1) the morphospecies approach is useful for detecting differences in species diversity of land-use types; (2) the concept of asymptotic diversity may not be necessary for land-use based biodiversity comparison; and (3) maximum diversity profiles are useful for determining the land-use conservation values in cases where pristine areas are not available.992.
Pablo M. Vergara Luis O. Meneses Audrey A. Grez Madelaine S. Quiroz Gerardo E. Soto Christian G. Pérez-Hernández Paola A. Diaz Ingo J. Hahn Andrés Fierro 《Landscape Ecology》2017,32(2):279-293
Context
Interactions between landscape-scale processes and fine-grained habitat heterogeneity are usually invoked to explain species occupancy in fragmented landscapes. In variegated landscapes, however, organisms face continuous variation in micro-habitat features, which makes necessary to consider ecologically meaningful estimates of habitat quality at different spatial scales.Objectives
We evaluated the spatial scales at which forest cover and tree quality make the greatest contribution to the occupancy of the long-horned beetle Microplophorus magellanicus (Coleoptera: Cerambycidae) in a variegated forest landscape.Methods
We used averaged data of tree quality (as derived from remote sensing estimates of the decay stage of single trees) and spatially independent pheromone-baited traps to model the occurrence probability as a function of multiple cross-scale combinations between forest cover and tree quality (with scales ranging between 50 and 400 m).Results
Model support and performance increased monotonically with the increasing scale at which tree quality was measured. Forest cover was not significant, and did not exhibit scale-specific effects on the occurrence probability of M. magellanicus. The interactive effect between tree quality and forest cover was stronger than the independent (additive) effects of tree quality and particularly forest cover. Significant interactions included tree quality measured at spatial scales ≥200 m, but cross-scale interactions occurred only in four of the seven best-supported models.Conclusions
M. magellanicus respond to the high-quality trees available in the landscape rather than to the amount of forest per se. Conservation of viable metapopulations of M. magellanicus should consider the quality of trees at spatial scales >200 m.993.
Context
Landscape graphs are widely used to model connectivity and to support decision-making in conservation planning. Compartmentalization methods applied to such graphs aim to define clusters of highly interconnected patches. Recent studies show that compartmentalization based on modularity is suitable, but it applies to non-weighted graphs whereas most landscape graphs involve weighted nodes and links.Objectives
We propose to adapt modularity computation to weighted landscape graphs and to validate the relevance of the resulting compartments using demographic or genetic data about the patches.Methods
A weighted adjacency matrix was designed to express potential fluxes, associating patch capacities and inter-patch distances. Eight weighting scenarios were compared. The statistical evaluation of each compartmentalization was based on Wilks’ Lambda. These methods were performed on a grassland network where patches are documented by annual densities of water voles in the Jura massif (France).Results
The scenarios in which patch capacity is assigned a small weight led to the more relevant results, giving high modularity values and low Wilks’ Lambda values. When considering a fixed number of compartments, we found a significant negative correlation between these two criteria. Comparison showed that compartments are ecologically more valid than graph components.Conclusions
The method proposed is suitable for designing ecologically functional areas from weighted landscape graphs. Maximum modularity values can serve as a guide for setting the parameters of the adjacency matrix.994.
995.
Annamária Kiss Typhaine Moreau Vincent Mirabet Cerasela Iliana Calugaru Arezki Boudaoud Pradeep Das 《Plant methods》2017,13(1):114
Background
Developmental biology has made great strides in recent years towards the quantification of cellular properties during development. This requires tissues to be imaged and segmented to generate computerised versions that can be easily analysed. In this context, one of the principal technical challenges remains the faithful detection of cellular contours, principally due to variations in image intensity throughout the tissue. Watershed segmentation methods are especially vulnerable to these variations, generating multiple errors due notably to the incorrect detection of the outer surface of the tissue.Results
We use the level set method (LSM) to improve the accuracy of the watershed segmentation in different ways. First, we detect the outer surface of the tissue, reducing the impact of low and variable contrast at the surface during imaging. Second, we demonstrate a new edge function for a level set, based on second order derivatives of the image, to segment individual cells. Finally, we also show that the LSM can be used to segment nuclei within the tissue.Conclusion
The watershed segmentation of the outer cell layer is demonstrably improved when coupled with the LSM-based surface detection step. The tool can also be used to improve watershed segmentation at cell-scale, as well as to segment nuclei within a tissue. The improved segmentation increases the quality of analysis, and the surface detected by our algorithm may be used to calculate local curvature or adapted for other uses, such as mathematical simulations.996.
997.
Powdery scab caused by Spongospora subterranea f. sp. subterranea (Sss) causes extensive losses in potato production systems globally. Two pot experiments were established in the greenhouse in summer 2013 and winter 2014 to evaluate the effectiveness of different soil chemicals, fumigant, amendments and biological control agents (BCAs) against Sss in the rhizospheric soil, potato roots and tubers. The study used visual assessment methods to assess the effect of treatments on root galling and zoosporangia production, and qPCR to measure Sss concentration in the soil and in the potato roots and tubers. All six soil treatments, namely metam sodium, fluazinam, ZincMax, calcium cyanamide, Biocult and a combination of Bacillus subtilis and Trichoderma asperellum recorded significantly (P < 0.05) lower numbers of zoosporangia in the roots compared to the untreated control. The same effect was observed on the concentration of Sss DNA in the roots at tuber initiation. A more diverse picture was obtained when root gall scores at tuber initiation and Sss DNA in the rhizospheric soil at tuber initiation and harvesting were compared. Significant differences (P < 0.05) were also noted in disease severity, disease incidence, and tuber yield between metam sodium, fluazinam, ZincMax, calcium cyanamide and the untreated control. Calcium cyanamide gave the highest tuber yield. The study demonstrated the potential of soil treatments such as metam sodium, fluazinam, ZincMax and calcium cyanamide in managing Sss in potatoes by reducing the pathogen both in the rhizospheric soil and the roots of the potato plant. 相似文献
998.
Three species of wireworms (Coleoptera: Elateridae) known to cause severe damage to potatoes in Europe and Asia have been introduced to Canada and are now well established as pests in the westernmost province of British Columbia (BC) (Agriotes obscurus and A. lineatus) and the eastern provinces of Nova Scotia and Prince Edward Island (A. obscurus, A. lineatus and A. sputator). Conventional insecticide-based efforts to control these invasive pests have had serious environmental impacts, or are failing to prevent severe economic damage from occurring to potatoes in some key potato production areas. Research toward developing an IPM programme for these exotic species has been completed or is underway in Canada, including the following: biological and ecological studies, development of monitoring and risk assessment programmes, and development of insecticidal and alternative control strategies. This research is summarized and implications for wireworm management in Europe are discussed. In addition to wireworms, one of the primary economic insect pests of potatoes in BC is the tuber flea beetle, Epitrix tuberis (Coleoptera: Chrysomelidae). The larvae of this beetle feed on developing or mature daughter tubers, producing surface channels or tracks and holes directly into the tuber. In the past, growers would apply 7–10 foliar sprays of broad-spectrum insecticides per growing season, which was not always successful in controlling this pest. In the EU, a newly identified flea beetle, Epitrix papa sp. n., as well as the North American species, E. cucumeris, have been identified as attacking potatoes initially in Portugal (2004) and later also in Spain. The potential spread of these emergent pests to various EU and non-EU countries is of concern, and if established would require the development of management strategies. The former development of a highly effective IPM programme developed in BC for E. tuberis is discussed, as is its potential for E. papa and E. cucumeris management in Europe. 相似文献
999.
Corné Kempenaar Thomas Been Johan Booij Frits van Evert Jean-Marie Michielsen Corné Kocks 《Potato Research》2017,60(3-4):295-305
Precision agriculture is a farming management concept based on observing, measuring and responding to inter- and intra-field variability in crops. In this paper, we focus on responding to intra-field variability in potato crops and analyse variable rate applications (VRAs). We made an overview of potential VRAs in potato crop management in The Netherlands. We identified 13 potential VRAs in potato, ranging from soil tillage to planting to crop care to selective harvest. We ranked them on availability of ‘proof of concept’ and on-farm test results. For five VRAs, we found test results allowing to make a cost-benefit assessment. These five VRAs were as follows: planting, soil herbicide weed control, N side dress, late blight control and haulm killing. They use one of two types of spatial data: soil maps or biomass index maps. Data on costs and savings of the VRAs showed that the investments in VRAs will pay off under practical conditions in The Netherlands. Savings on pesticide use and N-fertilizer use with the VRAs were on average about 25%, which benefits the environment too. We foresee a slow but gradual adoption of VRAs in potato production. More VRAs will become available given ongoing R&D. The perspectives of VRAs in potatoes are discussed. 相似文献
1000.
Didier Andrivon 《Potato Research》2017,60(3-4):389-400
This paper surveys issues and developments emerging from the keynotes and participants’ presentations during the 20th EAPR Triennial Conference, held in Versailles, France, in July 2017, and puts them into the perspective of the general theme of the meeting: potato facing global challenges. It reveals important and far-reaching innovations in many areas of potato research, including breeding (genomic selection, high-throughput phenotyping, genome editing), plant protection (biocontrol) and seed production, but also a general innovation approach that follows an incremental pathway (and maintains many existing lock-in situations), and favours substitution strategies over system re-conceptualisation and testing, however urgently needed these might be to achieve or improve the sustainability of potato production in the medium to long run. While the assets and importance of potato as both a wholesome food and an essential part of future worldwide food systems were once more evidenced and highlighted, the contributions also revealed (i) a growing dichotomy between research activities carried out in high-income vs in low- or moderate-income countries, respectively; (ii) some gaps in the assessment of the sustainability of potato production in the medium to long run (external inputs such as fertilisers or pesticides, land and energy use efficiency); and (iii) persistent weaknesses in the integration of individual solutions into complete, operational potato production systems. Some areas for future investigations are identified. 相似文献