Kaempferol alleviates the reduction of developmental competence during aging of porcine oocytes     

Xuerui Yao  Hao Jiang  Ying‐Hua Li  Qingshan Gao  Yong Nan Xu  Nam‐Hyung Kim 《Animal Science Journal》2019,90(11):1417-1425

Kaempferol (KAE) is a natural flavonoid present in different plant species and exhibits anti‐inflammatory, antioxidant, and anticancer therapeutic properties. In the present study, we investigated the influence and underlying mechanisms of KAE supplementation on porcine oocytes during in vitro aging. The results show that KAE treatment can alleviate the aging‐related reduction of developmental competence. We observed that the blastocyst production rate in aged oocytes treated with 0.1 μM KAE was significantly higher than in untreated aging oocytes (36.78 ± 0.86% vs. 27.55 ± 2.60%, respectively, p < .05). The KAE‐treated aging oocytes had significantly reduced levels of reactive oxygen species (p < .05). Furthermore, the mRNA levels of the embryonic pluripotency‐related genes Oct4, NANOG, and ITGA5 were significantly increased in blastocysts derived from KAE‐treated oocytes (p < .05). During excessive oocyte culture, KAE treatment maintained the mitochondrial membrane potential and reduced apoptosis; however, this was not observed in untreated aging oocytes. In conclusion, our results suggest that KAE treatment can alleviate the aging of porcine oocytes by reducing oxidative stress and improving mitochondrial function.  相似文献   

Retrobulbar vs peribulbar regional anesthesia techniques using bupivacaine in dogs     

Yael Shilo‐Benjamini  Peter J. Pascoe  David J. Maggs  Steven R. Hollingsworth  Ann R. Strom  Kathryn L. Good  Sara M. Thomasy  Philip H. Kass  Erik R. Wisner 《Veterinary ophthalmology》2019,22(2):183-191

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MicroPulse™ transscleral cyclophotocoagulation in the treatment of canine glaucoma: Preliminary results (12 dogs)     

Lionel Sebbag  Rachel A Allbaugh  Rachel A Strauss  Travis D Strong  Rita F Wehrman  Braidee C Foote  Gil Ben‐Shlomo 《Veterinary ophthalmology》2019,22(4):407-414

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Phenotype of macular corneal dystrophy in Labrador Retrievers: A multicenter study     

Claudia Busse  Christiane Kafarnik  Rose Linn‐Pearl  Christelle Volmer  Kaspar Matiasek  Johana E. Premont  Thomas Dulaurent  Jean‐Yves Douet  Ida Gilbert  Sari Jalomäki  Katrin Trost  Pierre‐Francois Isard  Ryan Boyd  Isabelle Raymond 《Veterinary ophthalmology》2019,22(3):294-304

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Quantitative,noninvasive assessment of intra‐ and extraocular perfusion by contrast‐enhanced ultrasonography and its clinical applicability in healthy dogs     

Klaas‐Ole Blohm  Katharina M. Hittmair  Alexander Tichy  Barbara Nell 《Veterinary ophthalmology》2019,22(6):767-777

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Bone marrow lesions of the distal condyles of the third metacarpal bone are common and not always related to lameness in sports and pleasure horses     

Ccile De Guio  Emilie Sgard‐Weisse  Aurlie Thomas‐Cancian  Michael Schramme 《Veterinary radiology & ultrasound》2019,60(2):167-175

The metacarpophalangeal joint is a common site of pain in horses. Little information is available about bone marrow lesions in the distal condyles of the third metacarpal bone in sports and pleasure horses. Aims of this prospective, retrospective, observational study were to evaluate the prevalence of bone marrow lesions in the distal condyles of the third metacarpal bone, to describe their anatomic distribution and to correlate lesions with the presence of lameness and the level and type of activity. All sports and pleasure horses undergoing standing low‐field MRI of the front fetlock region between 2009 and 2016 were included and divided into three lameness groups according to the results of diagnostic analgesia. Bone marrow lesions were analyzed and graded by one reader. Grades were compared between anatomical locations and between lameness groups. A total of 166 horses were sampled. The prevalence of bone marrow lesions was 76.5% (127/166). The dorsal aspects of the medial condyle (31%, 39/127) and of the sagittal ridge (28%, 36/127) were the most commonly affected locations. There was no significant difference between both forelimbs (lame limb and non‐lame limb) of the same horse. Lesion severity was neither significantly associated with the lameness group nor with the type or level of activity. The prevalence of bone marrow lesions in the distal condyles of the third metacarpal bone is high in this population and the clinical significance is not always clear. Further studies are required to elucidate the clinical significance of this finding in sports and pleasure horses.  相似文献   

Magnetic resonance imaging appearance of the brain and cervical spinal cord in an edema disease affected pig     

Lucía Dieste‐Prez  Tetyda P. Dobak  Federico R. Vilaplana Grosso  Wilhelmina Bergmann  Tijs J. Tobias 《Veterinary radiology & ultrasound》2019,60(2):E15-E19

A 7‐week‐old male pig was presented with signs of a central nervous system disorder. An MRI of the head and cervical spine was performed immediately after euthanasia. The MRI revealed multifocal bilaterally symmetric T2‐weighted hyperintense lesions in the brain and spinal cord, likely due to a toxic metabolic process. Histopathological examination supported the MRI findings and confirmed the diagnosis of edema disease due to Shiga‐like toxin produced by Escherichia coli. This is the first case published of the MRI findings in an edema disease affected pig.  相似文献   

Thoracic high resolution CT using the modified VetMousetrap™ device is a feasible method for diagnosing canine idiopathic pulmonary fibrosis in awake West Highland White Terriers     

Saila Holopainen  Elina Rautala  Liisa Lilja‐Maula  Hannes Lohi  Minna M. Rajamki  Anu K. Lappalainen 《Veterinary radiology & ultrasound》2019,60(5):525-532

Canine idiopathic pulmonary fibrosis is a chronic, progressive interstitial lung disease particularly prevalent in West Highland White Terriers. In the present prospective pilot study, we evaluated the feasibility of modified VetMousetrap? device in high resolution CT to detect idiopathic pulmonary fibrosis in West Highland White Terriers. Twelve awake West Highland White Terriers with canine idiopathic pulmonary fibrosis and 24 clinically healthy West Highland White Terriers were scanned using a helical dual slice scanner utilizing VetMousetrap? device without or with minimal chemical restraint with butorphanol. Three evaluators blindly assessed the images for image quality and the presence of canine idiopathic pulmonary fibrosis related imaging findings such as ground glass opacity and reticular opacities. Additionally, the attenuation of the lung was quantified with ImageJ software using histogram analysis of density over the lung fields. Computed tomography was successfully completed and motion artifact ranked in statistical analysis barely noticeable to mild in all dogs. The agreement between imaging findings and clinical status was very good with overall κ value 0.91 and percentage of agreement of 94%. There was also very good intraobserver (κ_range = 0.79‐0.91) and interobserver agreement (κ = 0.94). Moderate to severe ground glass opacity was present in all affected dogs. In the ImageJ analysis, a significant difference in lung attenuation between the study groups was observed. We conclude that modified VetMousetrap? device is applicable in diagnosing canine idiopathic pulmonary fibrosis in awake West Highland White Terriers avoiding anesthetic risk in these often severely hypoxic patients.  相似文献   

Diagnostic imaging for the assessment of acquired abdominal vascular diseases in small animals: A pictorial review     

Swan Specchi  Marc‐Andr d'Anjou 《Veterinary radiology & ultrasound》2019,60(6):613-632

Advances in interventional radiology and surgical techniques now allow complex abdominal diseases to be more successfully treated in small animals. Abdominal vascular alterations, acquired as individual process or as complication of other lesions such as neoplasia, can be life‐threatening or at least greatly limit curative interventions of underlying diseases. Computed tomography (CT) and high‐definition ultrasonography are now readily available in veterinary referral centers. Yet, there is little information currently available on the use of these modalities for the diagnosis and characterization of these vascular alterations. The purpose of this article is to review the CT and ultrasonographic findings of acquired vascular diseases in the abdomen of dogs and cats, using both the veterinary and human medicine literature as references, and highlighting essential concepts through figures.  相似文献   

Prevalence of pulmonary nodules in dogs with malignant neoplasia as determined by CT     

Christopher R. Lamb  James Whitlock  Andrew T. L. Foster‐Yeow 《Veterinary radiology & ultrasound》2019,60(3):300-305

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