Multivariate analysis and genetic diversity for morphometric and root textural quality traits in ashwagandha (Withania somnifera Dunal)     

R. Ramesh Kumar  L. Prasanna Anjaneya ReddyJ. Vinay Kumar  K. KomaraiahS. Purnanand  K.P. Sastry 《Industrial Crops and Products》2012,35(1):199-202

Ashwagandha (Withania somnifera) is an important medicinal plant and its dried roots are used in traditional systems of medicine. The market price of roots is determined by physical (textural) quality. Brittle roots with high starch and low fiber are considered to be superior because of ease in grinding. Genetic diversity studies based on root textural parameters have not been done so far. So the present study was designed to assess genetic diversity for morphometric traits and root textural quality parameters among two morphologically distinct groups: Poshita and Nagore. The PCA separated the morphometric and root texture variables distinctly into two different principal components: PC-1 and PC-2 respectively, indicating that both are negatively associated. All the morphotypes in Poshita group showed high positive loadings in PC-1 indicating that component genotypes are high root yielding. Nagore morphotypes were low yielding but the root texture was good. Clustering of morphotypes grouped Poshita and Nagore separately with high inter-cluster distances indicating that both groups are highly divergent from each other, suggesting that there is sufficient scope for varietal improvement through hybridization.  相似文献   

Immunogenicity of Major Cell Surface Protein(s) of Brucella melitensis Rev 1     

Mahajan NK  Kulshreshtha RC  Malik G  Dahiya JP 《Veterinary research communications》2005,29(3):189-199

Brucella melitensis Rev 1 organisms were salt-extracted and the cell surface proteins (BCSPs) were found to be mainly 39-42 kDa (group 2 porin proteins) in addition to 31.6, 32.5, 58.5 and 14.7 kDa proteins. DEAE-Sephadex anion-exchange column chromatography of BCSPs yielded fraction 1, which contained one major protein (39.8-42.0 kDa) and a minor protein (31.6 kDa). All these proteins were found to be immunogenic by Western blotting. Fraction 1 along with monophosphoryl lipid A and trehalose dicorynomycolate adjuvants as well as BCSPs alone induced significant (p < or = 0.05) protection in BALB/c mice. Both these immunizing agents produced almost equivalent protection to live B. melitensis Rev 1 vaccine at 15 and 30 days post challenge. Lymphocyte stimulation test as well as delayed-type hypersensitivity reaction revealed that both these preparations induced cell-mediated immune response. These preparations also induced humoral immune response as indicated by indirect ELISA. Neither of the immune responses was significantly less (p < or = 0.05) than that with live B. melitensis Rev 1 vaccine, except that their duration was short.  相似文献   

Effects of changes in N-fertilizer management on water quality trends at the watershed scale     

Vinay Nangia  Prasanna H. Gowda 《Agricultural Water Management》2010,97(11):1855-1860

In this study, the ADAPT (Agricultural Drainage and Pesticide Transport) model was calibrated and validated for monthly flow and nitrate-N losses, for the 2000-2004 period, from two minor agricultural watersheds in Seven Mile Creek (SMC-1 and SMC-2) in south-central Minnesota. First, the model was calibrated and validated using the water quality data from the SMC-1 and again independently validated with the SMC-2 dataset. The predicted monthly flow and associated nitrate-N losses agreed reasonably with the measured trends for both calibration (r² = 0.81 and 0.70 for flow and nitrate-N losses, respectively) and validation (r² = 0.85 and 0.78 for flow and nitrate-N losses from SMC-1, and 0.89 and 0.78 for flow and nitrate-N losses from SMC-2, respectively) periods. The model performed less satisfactorily for the snowmelt periods than it did for the entire simulation period. Using the calibrated model, long-term simulations were performed using climatic data from 1955 to 2004 to evaluate the effects of climatic variability and N application rates and timing on nitrate-N losses. The predicted nitrate-N losses were sensitive to N application rates and timing. A decrease in the fall N application rate from 179.3 to 112 kg/ha decreased nitrate-N losses by 23%. By changing application timing from fall to spring at a rate of 112 N kg/ha, nitrate-N losses decreased by 12%. The predicted nitrate-N losses showed a linear response to precipitation with larger losses generally associated with wet years. A 25% increase in mean annual precipitation would offset reductions in nitrate-N loss achieved using better N fertilizer management strategies described above.  相似文献   

Counterimmunoelectrophoresis detection of Brucella antigens in fetal stomachs of aborted sheep     

N K Mahajan  R C Kulshreshtha 《The Veterinary record》1986,119(18):455-456

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Studies on South Asian okra collection: Methodology for establishing a representative core set using characterization data   总被引：8，自引：0，他引：8  

R. K. Mahajan  I. S. Bisht  R. C. Agrawal  R. S. Rana 《Genetic Resources and Crop Evolution》1996,43(3):249-255

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Remaking the Global Economy – Edited by Jamie Peck and Henry Wai‐Chung Yeung     

Vinay K. Gidwani 《Growth and change》2006,37(2):325-328

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The use of ultrasonography, radiography, and surgery in the successful recovery from diaphragmatic hernia in a cow     

Saini NS  Kumar A  Mahajan SK  Sood AC 《The Canadian veterinary journal. La revue veterinaire canadienne》2007,48(7):757-759

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Characterization of seed storage proteins of urdbean (Vigna mungo)     

Ritu Mahajan  Sarla P. Malhotra  Randhir Singh 《Plant foods for human nutrition (Dordrecht, Netherlands)》1988,38(2):163-173

Dehulled and defatted flour of urdbean (Vigna mungo), Var T-9, contained 25% protein with maximum contribution by globulins (63%). Albumins and glutelins contributed 12% and 21% respectively, whereas prolamins were present only in traces (1%). Globulins were further fractionated into legumin and vicilin type proteins which were present in the ratio of 4:1. All the protein fractions were heterogenous in nature as revealed by high performance liquid chromatography. SDS-polyacrylamide gel electrophoresis revealed the total protein sample to contain 21 different components with molecular weights ranging from 8.92 to 117.49kd. Albumins, globulins, prolamins and glutelins resolved into 4, 8, 6 and 13 different sized components of molecular weights ranging from 10.23 to 25.53, 10.84 to 112.72, 10.33 to 51.52 and 8.91 to 112.72kd, respectively. Amino acid analysis of all fractions revealed that glutamic acid was present in maximum concentration followed by aspartic acid and lysine. Just like other pulse proteins, the urdbean proteins were also deficient in sulphur containing amino acids.  相似文献   

Mushroom tyrosinase: recent prospects   总被引：14，自引：0，他引：14  

Seo SY  Sharma VK  Sharma N 《Journal of agricultural and food chemistry》2003,51(10):2837-2853

Tyrosinase, also known as polyphenol oxidase, is a copper-containing enzyme, which is widely distributed in microorganisms, animals, and plants. Nowadays mushroom tyrosinase has become popular because it is readily available and useful in a number of applications. This work presents a study on the importance of tyrosinase, especially that derived from mushroom, and describes its biochemical character and inhibition and activation by the various chemicals obtained from natural and synthetic origins with its clinical and industrial importance in the recent prospects.  相似文献   

Chloroplast derived SSRs reveals genetic relationships in domesticated alliums and wild relatives     

Jayaswall  Kuldip  Sharma  Himanshu  Bhandawat  Abhishek  Sagar  R.  Jayaswal  Deepanshu  Kumar  Akash  Chaturvedi  Pratibha  Mahajan  Vijay  Kumar  Sanjay  Singh  Major 《Genetic Resources and Crop Evolution》2022,69(1):363-372

Genetic Resources and Crop Evolution - Alliums comprise of popular spices and used for various culinary purposes and nutraceuticals. Poor genetic characterization and scarce information regarding...  相似文献