桑悬浮细胞原生质体培养的研究   总被引：2，自引：1，他引：1  

陈爱玉  王勇 《蚕业科学》1993,19(3):135-138

采用继代培养三个月后的桑子叶悬浮细胞为材料,进行了原生质体的分离和培养。桑悬浮细胞在纤维素酶、果胶酶、半纤维素酶的混合溶液中,酶解获得产量高、活力强的原生质体。原生质体在K8p(附加6—BA、NAA、2,4—D、LH)液体培养基中,再生细胞经多次分裂,得到肉眼可见的小愈伤组织。再通过增殖继代培养,获得浅黄色、具有明显颗粒结构的愈伤组织,转至各种激素含量的MSB固体培养基中,尚未获得绿苗分化。  相似文献   

浅谈我省地方畜禽品种资源保护及开发利用     

王武器 《家畜生态》2002,23(1):56-59

近年来，我省先后从国内外引进了20多个鸡种，10多个牛种、羊种和猪种等，因其高效而占领了我省市场。同时存在着盲目引种，品种多乱、杂等问题。伴随着少数高产畜禽品种的普及推广，使各地固有的许多品种资源迅速减少或消失。因此，针对陕西畜禽品种资源的现状问题，并结合近年来畜牧业生产中的实际状况，特就如何搞好地方畜禽品种资源的保护及开发利用提出建议与对策。  相似文献   

Investigation of Cervical Patency and Uterine Appearance in Domestic Cats by Fluoroscopy and Scintigraphy     

K Chatdarong  N Kampa  E Axnr  C Linde‐Forsberg 《Reproduction in domestic animals》2002,37(5):275-281

The cervical patency of six domestic female cats was monitored under sedation by infusion of contrast medium (Omnipaque) into the cranial vagina during early oestrus, mid‐oestrus, late oestrus and interoestrus or a radiopharmaceutical (^99mTc‐HSA) during mid‐ and interoestrus in a non‐ovulatory oestrous cycle. The transport of the contrast medium or the radiopharmaceutical through the cervix and within the uterine horns was observed under fluoroscopy and with the aid of scintigraphy. In three of the queens, transcervical transport of contrast medium was demonstrated in all stages of oestrus, in one queen during mid‐oestrus, late oestrus and 1 day after oestrus, and in two queens only during late oestrus. The relations between the cervical patency to the contrast medium and the oestrous behaviour, cornification of the vaginal cells and the serum oestradiol‐17β concentration were evaluated, and a relationship was found between the cervical patency and the degree of vaginal cornification. Transcervical transport of the radiopharmaceutical was observed in three queens during mid‐oestrus. When the cervix was open, hysterography under a fluoroscope and hysteroscintigraphy were performed. The fluoroscopic and scintigraphic recordings revealed the patterns of the uterine contractions during oestrus in both ascending and descending directions, and the movement of the uterine contents back and forth between the uterine horns. The hysterograms were classified according to the shape of the uterine horns and the appearance of the endometrial lining. Spiral‐shaped uterine horns with a smooth inner contour were observed in two queens, and a corkscrew appearance with irregular filling defects in the uterine lumen was shown in two queens that had developed subclinical cystic endometrial hyperplasia. These findings demonstrated that fluids or particles deposited in the cranial vagina of the cat can be transported into the uterus during some stages of the oestrous cycle. The fluoroscopic and scintigraphic techniques developed in this study may be further modified to permit more detailed studies of uterine contractile patterns and sperm transport in the feline female reproductive tract. Hysterography proved useful to diagnose uterine disease. The information on cervical patency is of value also for the development of techniques for artificial insemination in this species, and should be studied also in the ovulatory cycle.  相似文献   

控制sch单养雄蚕品种雌雄蚕孵出比例的催青处理因素研究   总被引：1，自引：1，他引：0  

陈萍  鲁成  朱勇  向仲怀 《蚕业科学》2002,28(1):31-34

研究结果表明 :在RH 6 0 %状态下 ,控制性比的温度和下限时间负相关达到显著水平 ;控制性比的高温处理适于干燥状态下进行 ;处理后雌蚕孵化整齐度明显低于雄蚕孵化整齐度 ,雌雄蚕孵化整齐度均随处理温度的升高而下降 ,尤以雌蚕更为明显。由此提出了在RH 6 0 %状态下sch单养雄蚕品种控制性比的起点温度及温度、时间范围。认为高温催青用下限温度配合 1日收蚁法效果好 ,高温处理以后的胚胎适于在RH 80 %～ 85 %的湿度中保护  相似文献   

多效唑调控观赏竹生长的初步研究   总被引：1，自引：0，他引：1  

韩春  何奇江  李云昌  伍宏远 《竹子研究汇刊》2005,24(2):23-25

竹笋施用多效唑后,对其新梢日平均生长量及成竹后全竹高,枝下高、节间长、叶面积均有显著的抑制作用,注射处理在浓度300～800 mg·kg-1,喷施处理在浓度300～1000 mg·kg-1范围内,随处理浓度递增,抑制作用更为显著.应用多效唑能提高观赏竹的观赏价值,解决盆景制作的矮化及枝叶美化问题.  相似文献   

提高中锰白口铸铁韧性的研究     

李绪业  伍怡 《湖南农业大学学报(自然科学版)》1990,17(2)

为改善中锰白口铸铁的韧性以扩大其应用范围,本文探讨了C, Si, Mn等合金元素、变质处理和热处理工艺对中Mn白口铸铁组织和性能的影响.  相似文献   

Reproductive roles predict sexual dimorphism in internal and external morphology of lake whitefish,Coregonus clupeaformis     

S. J. Casselman  A. I. Schulte‐Hostedde 《Ecology of Freshwater Fish》2004,13(3):217-222

Abstract – The different reproductive roles of the sexes can predict the direction and magnitude of sexual dimorphism of external and internal morphology. Males should have enlarged structures that enhance the acquisition of mating opportunities, whereas females are predicted to have enlarged organs that are associated with the production of eggs. We tested these predictions in male and female lake whitefish, a species in which both sexes have similar overall body size and shape. After controlling for body size, male lake whitefish had significantly longer jaws and pectoral and pelvic fins, larger hearts, and more muscle than females. Sexual dimorphism in relative muscle mass may be one of the most fundamental morphological differences between males and females. Females had relatively heavier livers than males. Because the liver is important for the breakdown of fats and vitellogenesis, selection should favour an enlarged liver in females for the processing of energy and the production of large numbers of eggs.  相似文献   

增瘦剂对长×巴猪(F_1)染色体诱变的研究     

王勇强  汤怀斌 《郑州牧业工程高等专科学校学报》1990,(3)

本文采用外局血淋巴细胞培养及姊妹染色体分染技术,对埋植增瘦剂的长×巴猪(F_1)染色体进行了研究,结果表明,在所统计的细胞中,试验组正常二倍体细胞(2n=3.8)的百分率为71.43％,对照组为80.36％,差异极显著(P<0.01);试验组多倍体细胞数(8.33％)极显著地高于对照组(2.23％,P<0.01);试验组染色体断裂细胞百分率(10.67％)与对照组(8.00％)之间差异不显著(P>0.05);试验组姊妹染色体交换频率(1.88)极显著地高于对照组(0.76,P<0.01)。  相似文献   

微机综合自动化系统在大型泵站中的应用   总被引：8，自引：3，他引：5  

阚永庚  雍成林 《中国农村水利水电》2001,(3):40-42

水利部“94．8”项目之一“江都抽水机站机组监控关键技术”的实施，在国内首次实现了大型泵站“无人值守，少人值班”的新局面，该系统自动化调节功能，充分利用潮汐河流的特点，实现了泵站的优化调度，提高了运行效率，挖掘了泵站的潜在抽水能力，不仅节约了运行费用，而且还能增灌农田，显著地提高了经济效益和社会效益。  相似文献   

二十三个柚品种特性评价     

陈竹生  陈全友  江才伦  吴云伦  郭天池  聂华堂  计玉  甘俊新 《中国南方果树》1993,(2)

对1965年定植的23个柚(Citrus grandis Osbeck)品种进行了20多年的性状比较。筛选出矮化类型6个,早熟和晚熟类型各3个,丰产类型6个,品质优良类型11个。发现仅有部分柚品种富含维生素C;果汁中维生素C含量与酸含量呈明显负相关。证实长寿沙田柚中的冬瓜圈和古老钱两个选系比广西沙田柚更丰产优质;垫江白柚质优,但产量低。简述了各参试品种的利用前途。  相似文献