Identification and validation of QTLs for cold tolerance at the booting stage and other agronomic traits in a rice cross of a Japanese tolerant variety,Hananomai, and a NERICA parent,WAB56-104     

Cornelius Mbathi Wainaina  Daigo Makihara  Mitsuru Nakamura  Akihiro Ikeda  Taro Suzuki  Yuko Mizukami 《Plant Production Science》2018,21(2):132-143

In Africa, cold temperatures occur in the highlands of East and Southern Africa and in some areas of the Sahel region of West Africa leading to substantial rice yield losses. Cold tolerance (CT) at booting stage on basis of spikelet fertility after cold water irrigation was evaluated using F₂ population derived from a cross between temperate japonica, Hananomai, and tropical japonica, WAB56-104. Two Quantitative trait loci (QTLs) for CT were detected on chromosome 8 and 10 with enhanced effects on the trait coming from Hananomai and WAB56-104 allele, respectively. The QTLs explained 30% and 33% of phenotypic variation in spikelet fertility, respectively. CT was negatively correlated with panicle number (r = ?0.35, p < 0.01) and positively correlated with panicle weight (r = 0.61, p < 0.001). Selected BC₁F₄ and BC₁F₅ genotypes having homozygous alleles for both CT QTLs exhibited higher spikelet fertility under cold stress. The identified QTLs will be useful in the development of cold-tolerant varieties for production in high altitude areas through marker-assisted selection.  相似文献   

Inhibitory effects of edible marine algae extracts on degranulation of RBL-2H3 cells and mouse eosinophils     

Takashi Kimiya  Kazuhiro Ohtani  Setsuko Satoh  Yuko Abe  Yoshihiko Ogita  Hirohisa Kawakita  Hideyuki Hamada  Yuko Konishi  Satoshi Kubota  Akira Tominaga 《Fisheries Science》2008,74(5):1157-1165

Inhibitory effects on degranulation of rat basophilic leukemia (RBL-2H3) cells and mouse eosinophils by marine algae extracts were examined. More than 50% of the degranulation of RBL-2H3 cells was inhibited by water extracts of Ecklonia cava and Chrysymenia wrightii at a concentration of 100 μg/mL. More than 50% of the degranulation of RBL-2H3 cells was inhibited by methanol extracts of Petalonia binghamiae, Scytosiphon lomentaria, Undaria pinnatifida, Porphyra dentata, Codium fragile and Ulva japonica at a concentration of 200 μg/mL. Most inhibitory substances in the methanol extracts were partitioned into ethyl acetate and hexane layers. The ethyl acetate-partitioned layer of methanol extract of Petalonia binghamiae had higher inhibitory effects than the hexane-partitioned layer on the degranulation of RBL-2H3 cells. By contrast, the hexanepartitioned layer of the same extract had a higher inhibitory effect than the ethyl acetate-partitioned layer on the degranulation of mouse eosinophils. The ethyl acetate-partitioned layer of methanol extract of Petalonia binghamiae was further separated into eight fractions by silica gel column chromatography. Most inhibited the degranulation of RBL-2H3 cells, but not that of mouse eosinophils significantly. These results suggest methanol extract of Petalonia binghamiae contains materials that inhibit the degranulation of basophils and eosinophils differentially.  相似文献   

Removal of understory dwarf bamboo (<Emphasis Type="Italic">Sasa kurilensis</Emphasis>) induces changes in water-relations characteristics of overstory <Emphasis Type="Italic">Betula ermanii</Emphasis> trees     

Hiroaki T. Ishii  Tsuyoshi Kobayashi  Shigeru Uemura  Koichi Takahashi  Yuko T. Hanba  Akihiro Sumida  Toshihiko Hara 《Journal of Forest Research》2008,13(2):101-109

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Resource level as a proximate factor influencing fluctuations in male flower production in Cryptomeria japonica D. Don     

Yuko Miyazaki  Takeshi Osawa  Yoshiaki Waguchi 《Journal of Forest Research》2009,14(6):358-364

Airborne pollen of Cryptomeria japonica causes severe pollinosis, and the number of people suffering from pollinosis has increased rapidly during recent decades. Male flower production in C. japonica shows annual variability; thus, predicting male flower production for the following spring could be a countermeasure against pollinosis. We analyzed factors that explain fluctuations in male flower production of C. japonica according to parameters reflecting internal resource dynamics based on field observations. In addition, we carried out experiments to regulate available resource levels using artificial shading to test the effect of internal resource levels on the number of male flowers produced. We found that temperature and precipitation in the previous summer and a relative increase in male flower production in the previous year were negatively related, whereas sunshine duration in the previous summer and diameter at breast height were positively related to the current year increase in male flower production. The number of male flowers was significantly reduced by the shading treatment and was positively correlated with the amount of total non-structural carbohydrates in the root. These results suggest that understanding the parameters controlling internal resource levels could lead to more accurate predictions of male flower production.  相似文献   

Detection of indoleamine 2,3-dioxygenase 1-expressing cells in canine normal and tumor tissues     

Namiko IKEDA  Daiki KATO  Masaya TSUBOI  Ryohei YOSHITAKE  Shotaro ETO  Sho YOSHIMOTO  Masahiro SHINADA  Satoshi KAMOTO  Yuko HASHIMOTO  Yousuke TAKAHASHI  James CHAMBERS  Kazuyuki UCHIDA  Ryohei NISHIMURA  Takayuki NAKAGAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(12):1885

Cancer immunotherapy is a novel cancer treatment for canine tumors. Indoleamine 2,3-dioxygenase 1 (IDO1) is overexpressed in some human tumors and inhibits antitumor immunity. In this study, we comprehensively evaluated expression pattern of IDO1 and the nature of IDO1-expressing cells in canine normal and tumor tissues. In normal tissue samples, IDO1 expression was detected only in the lymph nodes, spleen, tonsil tissues, and colon tissues. In contrast, IDO1-positive tumor cells were observed in several tumor tissue types. This is the first study to evaluate IDO1 expression in canine normal and tumor tissues, and the results suggest that IDO1 is a promising target for novel cancer immunotherapy in dogs with tumors.  相似文献   

Suitability of Japanese codling as a raw material for surimi-based products revealed by primary sequence analysis of myosin heavy chain and thermal gel properties     

Watabe  Shugo  Ikeda  Daisuke  Mashiro  Takaki  Kagetakubo  Yuko  Takahashi  Yoshihiro  Uemura  Misaki  Mizusawa  Nanami  Koyama  Hiroki  Yasumoto  Ko  Jimbo  Mitsuru  Kan-no  Nobuhiro  Ueda  Tomohiro  Matsuoka  Yoko  Ueki  Nobuhiko  Wan  Jianrong 《Fisheries Science》2020,86(4):711-719

Fisheries Science - Complementary DNA encoding a part of myosin heavy chain was cloned from fast skeletal muscle of Japanese codling Physiculus japonicus. Japanese codling, a frequent catch off the...  相似文献   

Comparison of Effect of Brassinosteroid and Gibberellin Biosynthesis Inhibitors on Growth of Rice Seedlings     

Matusmoto Tadashi  Yamada Kazuhiro  Yoshizawa Yuko  Oh Keimei 《水稻科学》2016,23(1):51

Brassinosteroid(BR) and gibberellin(GA) are two predominant plant hormones that regulate plant cell elongation. Mutants disrupt the biosynthesis of these hormones and display different degrees of dwarf phenotypes in rice. Although the role of each plant hormone in promoting the longitudinal growth of plants has been extensively studied using genetic methods, their relationship is still poorly understood. In this study, we used two specific inhibitors targeting BR and GA biosynthesis to investigate the roles of BR and GA in growth of rice seedlings. Yucaizol, a specific inhibitor of BR biosynthesis, and Trinexapac-ethyl, a commercially available inhibitor of GA biosynthesis, were used. The effect of Yucaizol on rice seedlings indicated that Yucaizol significantly retarded stem elongation. The IC_(50) value was found to be approximately 0.8 μmol/L. Yucaizol also induced small leaf angle phenocopy in rice seedlings, similarly to BR-deficient rice, while Trinexapac-ethyl did not. When Yucaizol combined with Trinexapac-ethyl was applied to the rice plants, the mixture of these two inhibitors retarded stem elongation of rice at lower doses. Our results suggest that the use of a BR biosynthesis inhibitor combined with a GA biosynthesis inhibitor may be useful in the development of new technologies for controlling rice plant height.  相似文献   

Prevalence of antimicrobial resistance in bacteria isolated from Great Cormorants (Phalacrocorax carbo hanedae) in Japan     

Justice Opare ODOI  Michiyo SUGIYAMA  Yuko KITAMURA  Akiko SUDO  Tsutomu OMATSU  Tetsuo ASAI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(8):1191

Wild birds are recognized as disseminators of antimicrobial-resistant (AMR) bacteria into the environment. Here, we isolated AMR indicator bacteria from 198 Great Cormorant cloacal swabs collected in Shiga (n=90), Oita (n=52), Gifu (n=29), and Gunma (n=27) Prefectures, Japan, in 2018 and 2019. In total, 198 Aeromonas spp. and 194 Escherichia spp. were isolated, and their antimicrobial susceptibility was examined. Aeromonas spp. were resistant to colistin (8.6%), nalidixic acid (4%), and other antimicrobials (<2%), with 3.0% positivity for mcr-3. Escherichia spp. showed resistance to colistin (3.1%), ampicillin (2.6%), tetracycline (2.1%), and other antimicrobials (<2%). This study shows the presence of AMR bacteria in Great Cormorants, indicating that these birds potentially disseminate AMR bacteria.  相似文献   

Effects of temperature, salinity and their interaction on growth of toxic Ostreopsis sp. 1 and Ostreopsis sp. 6 (Dinophyceae) isolated from Japanese coastal waters     

Yuko Tanimoto  Haruo Yamaguchi  Takamichi Yoshimatsu  Shinya Sato  Masao Adachi 《Fisheries Science》2013,79(2):285-291

Some species belonging to Ostreopsis, a benthic dinoflagellate genus, are known to produce palytoxin analogues. Around the coastal regions of Japan, the toxic Ostreopsis sp. 1 and Ostreopsis sp. 6 which are genetically divergent from other species of Ostreopsis are present from the southern to northern regions and in the southern region, respectively. The present study examined the growth responses of these strains to seven temperatures (15–35 °C) in combination with five salinities (20–40) and discusses the effects of temperature and salinity on their distribution and bloom dynamics in Japan. Tolerable temperatures and salinities ranged 15–30 °C and 25–40 for Ostreopsis sp. 1, and 17.5–30 °C and 20–40 for Ostreopsis sp. 6. The optimal temperature ranges which gave growth rates of >90 % of maximal growth rate of each strain were 22–25 °C for Ostreopsis sp. 1 and 24–30 °C for Ostreopsis sp. 6. Therefore, Ostreopsis sp. 1 is putatively tolerant to lower temperatures and thus possesses adaptability to colder waters of relatively higher latitude regions of Japan, whereas Ostreopsis sp. 6 presumably possesses adaptability to warmer waters of the southern region. We conclude that growth responses of Japanese toxic Ostreopsis sp. 1 and Ostreopsis sp. 6 to temperature-salinity affect their distribution and bloom dynamics in Japan.  相似文献   

Effects of retinoic acid and hydrogen peroxide on sterol regulatory element-binding protein-1a activation during adipogenic differentiation of 3T3-L1 cells     

Abd Eldaim MA  Okamatsu-Ogura Y  Terao A  Kimura K 《The Japanese journal of veterinary research》2010,58(3-4):149-154

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