Improving the efficiency of wild Pecten maximus spat collection: potential effects of spat bag design and of species temporarily settling in spat bags     

Deborah Cashmore  Mark McG. Learmouth  John T. MacMillan 《Aquaculture (Amsterdam, Netherlands)》1998,160(3-4):273-282

The single and combined effects of external bag size and external bag mesh size upon the collection and retention of wild King Scallop spat (Pecten maximus L.), other scallop species (Aequipecten opercularis) and other animal groups were investigated in a collection area off the west coast of Scotland. Collection was assessed after 5 weeks and at harvest (after 20 weeks). After 5 weeks, 90% of collected animals consisted of scallops (P. maximus and A. opercularis), Mytilus edulis (Mollusca: Bivalvia) and Lacuna vincta (Mollusca: Gastropoda). One-way analysis of variance on count data obtained after 5 weeks showed that the larger bag and mesh size treatments had collected significantly greater numbers of scallop spat when numbers collected were compared to the quantity of material used for each treatment (P<0.05). This was partly attributed to significantly greater numbers of L. vincta crawling juveniles temporarily recruiting in the smaller bag and mesh treatments (P<0.05). Similar trends of spat abundance in treatment bags were observed at harvest, although all treatments had scallop spat retention efficiencies of less than 12%. The importance of the structural design of spat bags for spat collection and the effects of incidentally collected non-predatory species upon the collection of scallop spat are discussed.  相似文献   

Crafting an Antidote to Fast Fashion: The Case of Toronto's Independent Fashion Design Sector     

Deborah Leslie  Shauna Brail  Mia Hunt 《Growth and change》2014,45(2):222-239

The fashion industry has undergone a profound transformation in business practices and production systems over the past several decades. These shifts include the globalisation of production chains and the emergence of a new model of “fast fashion.” This paper investigates the response of independent fashion designers in Toronto, Canada to the growing competition posed by fast fashion. It identifies a number of strategies utilised by designers to compete, arguing that they are increasingly adopting a new model of “slow fashion,” which opens up possibilities for forging locally and ethically based relationships in the fashion sector.  相似文献   

Measurements of microvascular perfusion in healthy anesthetized dogs using orthogonal polarization spectral imaging     

Deborah C. Silverstein DVM  DACVECC  Antonio Pruett‐Saratan II  Kenneth J. Drobatz DVM  MSCE  DACVECC  DACVIM 《Journal of Veterinary Emergency and Critical Care》2009,19(6):579-587

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Growth pattern and partial proteome of Mycobacterium avium subsp. paratuberculosis during the stress response to hypoxia and nutrient starvation     

Gumber S  Taylor DL  Marsh IB  Whittington RJ 《Veterinary microbiology》2009,133(4):344-357

Mycobacterium avium subsp. paratuberculosis is an important pathogen that causes Johne's disease in animals and has been implicated in Crohn's disease in man yet few data exist on its physiological adaptation in either the host or the environment. In this study, the proteomic responses of the two distinct strains of M. a. paratuberculosis, cattle (C) and sheep (S), to hypoxia and starvation were studied in vitro. Nutrient starvation inhibited growth of both strains and was lethal for S strain after 12 weeks. Hypoxia induced a state of very low metabolic activity but rapid resuscitation occurred upon restoration of an aerobic atmosphere, consistent with the dormancy response of other mycobacteria. A total of 55 protein spots differentially expressed in response to starvation and/or hypoxic stress in one or both strains were identified from 2D gels and classified based on biological function. Antioxidant enzymes, oxidoreducatse enzymes and proteins involved in amino acid metabolism, fatty acid metabolism, ATP and purine biosynthesis, proteolysis, cell wall synthesis, protein synthesis, signal recognition and hypothetical proteins with putative functions including dormancy response regulators and universal stress proteins were identified. These proteins are potential screening targets for future diagnosis, prevention and control of M. a. paratuberculosis infection and their identification will assist understanding the pathogenesis of diseases caused by this organism.  相似文献   

Determination of irritant threshold concentrations to weeds,trees and grasses through serial dilutions in intradermal testing on healthy clinically nonallergic dogs     

Cynthia L. Bauer  Patrick Hensel  Michaela Austel  Deborah Keys 《Veterinary dermatology》2010,21(2):192-197

Irritant threshold concentration (ITC) for intradermal testing (IDT) was determined in 31 healthy, clinically nonallergic dogs. Twenty‐three allergens were tested at five variable concentrations ranging from 1000 to 8000 PNU/mL. To distinguish irritant reactions from subclinical IgE‐mediated hypersensitivities, serum allergy testing was performed. ITCs were determined by evaluating the lowest concentration to which no dogs (0% cut‐off) and to which at least 10% of dogs (≥10% cut‐off) reacted. ITCs at the 0% cut‐off were: 1000 PNU/mL (Johnson grass), 2000 PNU/mL (Ash, Lamb’s Quarter and Bermuda), 3000 PNU/mL (Bahia, Rye, Pig Weed and Virginia Oak), 4000 PNU/mL (Marsh Elder and Maple), 5000 PNU/mL (Sorrel sheep) and 7000 PNU/mL (Cocklebur and Black Willow). ITC for Dog Fennel, Box Elder and Red Cedar was <1000 PNU/mL. ITCs at the ≥10% cut‐off were: 2500 PNU/mL (Johnson), 3000 PNU/mL (Box Elder), 5000 PNU/mL (Bahia), 6000 PNU/mL (Pigweed and Marsh Elder) and 8000 PNU/mL (Virginia Oak and Black Willow). For all other allergens, the ITC was >8000 PNU/mL and could not be determined. No significant agreement between positive values was found for the same allergen on IDT and serum allergy testing for each dog suggesting reactions caused by the determined ITCs are less likely subclinical IgE‐mediated reactions. These results suggest that ITCs may vary, also they may be very high for the allergens tested and that higher test concentrations may be used for IDT for the tested allergens without inducing an irritant reaction. Further studies are needed to evaluate the benefit of higher IDT concentrations in atopic dogs.  相似文献   

Comparison of regular insulin infusion doses in critically ill diabetic cats: 29 cases (1999–2007)     

Melissa A. Claus DVM  Deborah C. Silverstein DVM  DACVECC  Frances S. Shofer PhD  Matthew S. Mellema PhD  DVM 《Journal of Veterinary Emergency and Critical Care》2010,20(5):509-517

Objective – To compare biochemical parameters, neurologic changes, length of hospital stay, and clinical improvement in 3 groups of cats with diabetic ketosis/diabetic ketoacidosis (DK/DKA) prescribed varied doses of regular insulin as a continuous rate of infusion (CRI). Design – Retrospective study. Setting – University teaching hospital. Animals – Twenty‐nine client‐owned cats with DK/DKA prescribed a regular insulin CRI. Interventions – Cats were grouped as follows: 7 cats each in Group 1 and 2, (prescribed 1.1 and 2.2 U/kg/d, respectively), and 15 cats in Group 3 (prescribed increasing doses as needed). Measurements and Main Results – None of the groups received the total prescribed dose of insulin. The mean actual dose administered/kg/d ranged from 0.30 (0.21) to 0.87 (0.32) U/kg/d in Groups 1, 2, and 3. There was no difference in mean minimum blood glucose (BG) per 4 hours or change in BG from baseline per 4 hours between Groups 1 and 2 (P=0.63, 0.50). There was no difference between groups regarding the time required to reach a BG ≤13.9 mmol/L (250 mg/dL), serum phosphorus or potassium concentrations relative to baseline values (P=0.53, 0.90), length of time until urine or serum ketones were no longer detected (P=0.73), the animal commenced eating (P=0.24), or length of hospital stay (P=0.63). Four of the cats had declining mentation during hospitalization; there were no relationships between osmolality at presentation, either prescribed or administered insulin dose, and mentation changes. Three of the 4 cats with declining mentation survived. Twenty‐seven of the 29 cats (93%) survived to discharge. Conclusions – In this study, prescribing the published canine dose (2.2 U/kg/d) of regular insulin to cats with DK/DKA does not appear to increase the frequency of adverse neurologic or biochemical sequelae compared with cats that are prescribed the published cat dose (1.1 U/kg/d). The use of a sliding scale for determination of infusion rates significantly reduces the amount of insulin cats receive in this setting. Determination of whether adverse sequelae would occur more frequently if cats with DK/DKA received the full insulin prescribed doses of 1.1, 2.2, or >2.2 U/kg/d is warranted. Further controlled studies are necessary to determine if higher doses of insulin are associated with beneficial effects on morbidity or mortality.  相似文献   

Functional habitat connectivity for beach mice depends on perceived predation risk     

Elliot B. Wilkinson  Lyn C. Branch  Deborah L. Miller 《Landscape Ecology》2013,28(3):547-558

Landscape features that promote animal movement contribute to functional habitat connectivity. Factors that affect the use of landscape features, such as predation risk, may alter functional connectivity. We identify factors important to functional habitat connectivity by quantifying movement patterns of the Santa Rosa beach mouse (Peromyscus polionotus leucocephalus) in relation to landscape features and by examining how ambient perceived predation risk, which is altered by moon phase, interacts with landscape features. We use track paths across the sand to relate the probability that beach mice cross gaps between vegetation patches to gap width, patch quality, landscape context and moon phase. Overall activity levels were lower during full versus new moon nights, demonstrating that beach mice respond negatively to moonlight. Gap crossing was more likely during new moon nights (25 % of gaps crossed vs. 7 % during full moon nights), and across narrower gaps (<8.38 m) that led to larger vegetation patches (>11.75 m²). This study suggests that vegetation recovery is necessary for functional connectivity in post-hurricane landscapes commonly inhabited by beach mice and provides initial guidelines for restoring landscape connectivity. More broadly, this study highlights the importance of considering predation risk when quantifying landscape connectivity, as landscape features that facilitate connectivity when predation risk is low may be ineffective if predation risk increases over time or across space.  相似文献   

Differences in nitrogen‐assimilating enzyme activity in halophyte species are habitat‐related          下载免费PDF全文

Grace M. Cott  Deborah V. Chapman  Marcel A. K. Jansen 《植物养料与土壤学杂志》2014,177(5):705-713

The chemical form and content of available nitrogen (N) in salt marsh substrates varies considerably. On the western coast of Ireland, habitats designated as Ombrogenic Atlantic salt marshes were formed on ombrogenic peat substrate. The peat substrate in these systems has three times more ammonium than substrate from adjacent salt marsh habitats on sand and mud substrate. This study examined the extent to which the high concentration of ammonium in peat salt marsh substrate influences the N‐ assimilating enzyme activity of halophytes and the extent to which N metabolism differs between species. Specifically, this work investigated whether plants from peat salt marshes are more likely to assimilate ammonium than plants from non‐peat substrates. Four halophyte plant species—Armeria maritima, Aster tripolium, Plantago maritime, and Triglochin maritime—were sampled from various saltmarsh habitats including three sites on peat substrate and three on non‐peat substrate, comprising sand, mud and sand/mud. The activities of N‐metabolising enzymes—glutamine synthetase (GS), glutamate synthase, glutamate dehydrogenase (GDH), and nitrate reductase (NR)—were quantified in shoot and root parts. Root GS activity in Armeria maritima and shoot GS activity in Triglochin maritima were positively correlated with increasing soil ammonium levels. Root NR activity in Aster tripolium and shoot NR activity in Plantago maritima were significantly higher in plants grown on non‐peat substrates than peat substrates. The shoot : root GS activity ratio in Triglochin maritima on peat substrate was more than double the ratio on non‐peat substrates. It is concluded that all species tested displayed differences in N‐metabolising activities depending on the chemical form and/or concentration of N in the substrate, while three out of the four species were capable of taking advantage of the high levels of ammonium in peat substrates.  相似文献   

An evaluation of the influence of stock origin and out-migration history on the disease susceptibility and survival of juvenile Chinook salmon     

Dietrich JP  Boylen DA  Thompson DE  Loboschefsky EJ  Bravo CF  Spangenberg DK  Ylitalo GM  Collier TK  Fryer DS  Arkoosh MR  Loge FJ 《Journal of aquatic animal health》2011,23(1):35-47

Various methods have been developed to mitigate the adverse effects of the Federal Columbia River Power System on juvenile Pacific salmon out-migrating through the Columbia River basin. In this study, we found that hatchery-reared spring Chinook salmon Oncorhynchus tshawytscha in the river are in varying degrees of health, which may affect delayed mortality and the assessment of the effectiveness of management actions to recover listed stocks (e.g., barging fish downstream versus leaving fish in the river). A laboratory disease challenge with Listonella anguillarum was completed on fish from Rapid River Hatchery and Dworshak National Fish Hatchery (NFH) with different out-migration histories: (1) transported by barge, (2) removed from the river before barging, or (3) left to travel in-river. Barged fish from Rapid River Hatchery experienced less mortality than fish from Dworshak NFH. No statistical differences were found between the hatcheries with fish that had in-river out-migration histories. We suggest that the stressors and low survival associated with out-migration through the hydropower system eliminated any differences that could have been present. However, 18-25% of the fish that were barged or collected before barging died in the laboratory before the disease challenge, compared with less than 2% of those that traveled in-river. Owing to disproportionate prechallenge mortality, the disease-challenged populations may have been biased; thus, they were also considered together with the prechallenge mortalities. The synthesis of prechallenge and disease-challenged mortalities and health characteristics evaluated during out-migration indicated that the benefit of barging was not consistent between the hatcheries. This finding agrees with adult survival and delayed mortality estimates for the individual hatcheries determined from adult returns. The results suggest that the health status of fish and their history before entering the hydropower system (hatchery of origin and out-migration path) are critical variables affecting the conclusions drawn from studies that evaluate mitigation strategies.  相似文献   

Comparison of isothiocyanate metabolite levels and histone deacetylase activity in human subjects consuming broccoli sprouts or broccoli supplement     

Clarke JD  Riedl K  Bella D  Schwartz SJ  Stevens JF  Ho E 《Journal of agricultural and food chemistry》2011,59(20):10955-10963

Increased consumption of cruciferous vegetables such as broccoli may reduce the risk of various cancers. Myrosinase is required to convert dietary glucosinolates from broccoli into bioactive isothiocyanates. We evaluated isothiocyanate excretion profiles in healthy subjects who consumed broccoli sprouts or broccoli supplement (no myrosinase) with equivalent glucosinolate content. Urinary metabolites of two major isothiocyanates, sulforaphane and erucin, were measured by liquid chromatography coupled with tandem mass spectrometry. Peak excretion of sulforaphane and erucin was higher and occurred sooner in subjects who consumed broccoli sprouts as compared to subjects who consumed the supplement. A subject-dependent shift in the ratio of urinary sulforaphane to erucin metabolites was observed in both groups, indicating conversion of sulforaphane to erucin. Lower histone deacetylase activity was observed in the peripheral blood mononuclear cells only in subjects consuming sprouts. Fresh broccoli sprouts differ from broccoli supplements in regards to excretion of isothiocyanates and bioactivity in human subjects.  相似文献