地高辛标记质粒探针监测卡那霉素耐药性的研究   总被引：6，自引：0，他引：6  

杨汉春  赵静  刘金华  查振林  陈艳红 《中国农业科学》2001,34(1):77-80

 用 Eco R 单酶切卡那霉素抗性质粒 ,经 DNA纯化回收 4.34 1kb的目的基因片段。以随机引物法 ,用地高辛进行标记 ,制备成探针 ,成功建立了斑点杂交法和菌落原位杂交法用于检测猪源大肠杆菌对卡那霉素的耐药性 ,结果表明与药敏试验阳性的符合率为 10 0 % ,说明建立的探针技术可以用于猪源大肠杆菌对卡那霉素的耐药性监测。  相似文献   

Functional links between Aβ toxicity, endocytic trafficking, and Alzheimer's disease risk factors in yeast     

Treusch S  Hamamichi S  Goodman JL  Matlack KE  Chung CY  Baru V  Shulman JM  Parrado A  Bevis BJ  Valastyan JS  Han H  Lindhagen-Persson M  Reiman EM  Evans DA  Bennett DA  Olofsson A  DeJager PL  Tanzi RE  Caldwell KA  Caldwell GA  Lindquist S 《Science (New York, N.Y.)》2011,334(6060):1241-1245

Aβ (beta-amyloid peptide) is an important contributor to Alzheimer's disease (AD). We modeled Aβ toxicity in yeast by directing the peptide to the secretory pathway. A genome-wide screen for toxicity modifiers identified the yeast homolog of phosphatidylinositol binding clathrin assembly protein (PICALM) and other endocytic factors connected to AD whose relationship to Aβ was previously unknown. The factors identified in yeast modified Aβ toxicity in glutamatergic neurons of Caenorhabditis elegans and in primary rat cortical neurons. In yeast, Aβ impaired the endocytic trafficking of a plasma membrane receptor, which was ameliorated by endocytic pathway factors identified in the yeast screen. Thus, links between Aβ, endocytosis, and human AD risk factors can be ascertained with yeast as a model system.  相似文献   

Clonal production and organization of inhibitory interneurons in the neocortex     

Brown KN  Chen S  Han Z  Lu CH  Tan X  Zhang XJ  Ding L  Lopez-Cruz A  Saur D  Anderson SA  Huang K  Shi SH 《Science (New York, N.Y.)》2011,334(6055):480-486

The neocortex contains excitatory neurons and inhibitory interneurons. Clones of neocortical excitatory neurons originating from the same progenitor cell are spatially organized and contribute to the formation of functional microcircuits. In contrast, relatively little is known about the production and organization of neocortical inhibitory interneurons. We found that neocortical inhibitory interneurons were produced as spatially organized clonal units in the developing ventral telencephalon. Furthermore, clonally related interneurons did not randomly disperse but formed spatially isolated clusters in the neocortex. Individual clonal clusters consisting of interneurons expressing the same or distinct neurochemical markers exhibited clear vertical or horizontal organization. These results suggest that the lineage relationship plays a pivotal role in the organization of inhibitory interneurons in the neocortex.  相似文献   

Polycrystalline CVD Diamond Films with High Electrical Mobility     

Plano MA  Landstrass MI  Pan LS  Han S  Kania DR  McWilliams S  Ager JW 《Science (New York, N.Y.)》1993,260(5112):1310-1312

Advances in the deposition process have led to dramatic improvements in the electronic properties of polycrystalline diamond films produced by chemical vapor deposition (CVD). It is now possible to produce CVD diamond with properties approaching those of IIa natural diamonds. The combined electron-hole mobility, as measured by transient photoconductivity at low carrier density, is 4000 square centimeters per volt per second at an electric field of 200 volts per centimeter and is comparable to that of the best single-crystal IIa natural diamonds. Carrier lifetimes measured under the same conditions are 150 picoseconds for the CVD diamond and 300 picoseconds for single-crystal diamond. The collection distance at a field of 10 kilovolts per centimeter is 15 micrometers for the CVD diamond as compared to 30 micrometers for natural diamonds. The electrical qualities appear to correlate with the width of the diamond Raman peak. Also, although the collection distance at the highest fields in the films nearly equals the average grain size, there is no evidence of deleterious grain boundary effects.  相似文献   

植物的离子吸收动力学研究的现状和前景(综述)     

韩振海  王永章  王倩 《中国农业大学学报》1994,(4):381-388

本文以文献及作者在果树上的研究为基础,对植物离子吸收动力学的基本概念、研究方法、影响动力学参数的主要因素和在果树营养研究中的应用,以及离子吸收动力学的意义和发展前景进行了述评。  相似文献   

橡胶树新种质主要性状鉴定评价报告(Ⅰ)--1986年定植大田新种质鉴定评价   总被引：5，自引：0，他引：5  

胡彦师  曾霞  程汉  方家林  黄华孙 《热带农业科学》2005,25(5):1-4,23

对我国1986年定植于大田鉴定评价圃的14份橡胶树新种质的产量、乳管数及生长势等性状进行了鉴定。结果表明：1、3、7和9号的生长势优于对照品种RRIM600，其中，7、9号种质的平均茎围与对照差异达到显著；Amazon野生橡胶树种质干胶产量均不及对照品种RRIM600，最好的1份种质平均干胶产量3．6kg／株，为对照品种RRIM600的88．7％，但二者差异不显著；除10、12和13号3份种质的乳管数相对较优外，其它新种质的乳管列数和乳管个数均明显少于对照。综合评价1、3、7和9号种质生长较快，9号种质产胶潜力相对较好，10、12和13号3份种质其乳管数相对较优。  相似文献   

双峰驼血液蛋白多态性遗传的研究(摘要)     

门正明  韩建林  张月周  李积友 《甘肃农业大学学报》1990,(1)

作者对136峰双峰驼的血液蛋白多态性作了分析,通过聚丙烯酰胺凝胶电泳测定血红蛋白(Hb)、血清白蛋白(Alb)、后白蛋白(Pa)和运转蛋白(Tf)得出的结果表明:双峰驼的Hb,Alb,Pa和Tf只有一种基因型,而且全部纯合,不表现多态性现象。同时还发现6个月以下的幼驼没有驼羔血红蛋白存在。  相似文献   

添加己酸和盐酸对玉米秸秆青贮饲料质量的影响   总被引：7，自引：0，他引：7  

席兴军  韩鲁佳  原慎一郎  甘利雅弘 《中国农业大学学报》2002,7(6):54-60

通过测定盐酸、己酸处理的玉米秸秆青贮饲料的pH值、含水率、有机酸含量及组成、干物质消失率、NDF瘤胃降解率、干物质回收率和青贮饲料化学成分，研究了盐酸、己酸的处理对玉米秸秆青贮饲料质量的影响。试验结果表明：在玉米秸秆青贮饲料中添加盐酸，降低了青贮饲料中乳酸占总酸的比例和干物质回收率，并使青贮饲料的细胞壁物质含量显著增加，明显降低了玉米秸秆青贮饲料的质量；己酸的添加对青贮饲料质量无显著影响(P＞0．05)；盐酸和己酸的共同处理除了明显改善青贮饲料的色泽、气味和质地外，还可明显降低青贮饲料中OB，ADF和ADL的含量(P＞0．05)，显著提高了青贮饲料的营养价值。  相似文献   

纽曼与洪堡大学理念比较     

韩芳  李维喆 《高等农业教育》2007,(2):87-89

纽曼与洪堡的大学理念深刻影响了19世纪以来世界高等教育的发展与变革,本文在分析两位教育家大学理念的思想核心的基础上,从大学的界定、职能、培养目标、教学、师生关系五个方面对两种大学理念进行比较,并指出两种大学理念的影响和不足,以期充分理解两位教育家的思想内涵并促进对当代高等教育发展现状的理解和改革.  相似文献   

蔬菜高效施药装置药量专家系统测试     

马伟  王秀  沈瀚  翟长远  秦贵  张睿 《中国蔬菜》2011,(Z1)

为了满足蔬菜高效施药的精确性和便捷性,借助信息技术手段将专家知识库存贮在单片机芯片中,通过快捷的选择调用达到蔬菜高效施药的目的。本文介绍了高效施药装置专家系统的工作原理,并通过试验验证了其理论施药量和实际控制值具有较好的一致性。  相似文献