Targeted genome editing across species using ZFNs and TALENs   总被引：2，自引：0，他引：2  

Wood AJ  Lo TW  Zeitler B  Pickle CS  Ralston EJ  Lee AH  Amora R  Miller JC  Leung E  Meng X  Zhang L  Rebar EJ  Gregory PD  Urnov FD  Meyer BJ 《Science (New York, N.Y.)》2011,333(6040):307

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Cell growth of ovarian cancer cells is stimulated by xenoestrogens through an estrogen-dependent pathway, but their stimulation of cell growth appears not to be involved in the activation of the mitogen-activated protein kinases ERK-1 and p38     

Park SH  Kim KY  An BS  Choi JH  Jeung EB  Leung PC  Choi KC 《The Journal of reproduction and development》2009,55(1):23-29

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Optimal pest management: A reproductive pollutant perspective     

Run Yu  Pingsun Leung 《国际虫害防治杂志》2013,59(3):155-166

Abstract

Regarding pests as reproductive pollutants, pest management can be considered as an optimal stock management problem similar to that faced in fishery and forestry. Taking this view, an optimal control model with N heterogeneous farmers sharing a mobile pest is developed to investigate the conventional pest management tactic on a farm-by-farm basis and the innovative area-wide pest management tactic on a regional basis. The study results indicate that area-wide pest management (APM) is superior to farm-by-farm pest management in the presence of pest movement. We proceed to examine the stability of the cooperative solution under APM by formulating the pest management problem as a repeated game of infinite duration. The results suggest that a one-off pest suppression campaign can be a practical strategy for initiating APM programs.  相似文献   

Anovulation in postpartum suckled beef cows. I. Associations among size and numbers of ovarian follicles, uterine involution, and hormones in serum and follicular fluid     

L J Spicer  K Leung  E M Convey  J Gunther  R E Short  H A Tucker 《Journal of animal science》1986,62(3):734-741

Changes in sizes and numbers of ovarian antral follicles, uterine size and weight, serum hormones, and frequency and duration of suckling were examined during the postpartum anovulatory period in primiparous, suckled beef cows. Twenty-one anovulatory, suckled cows (n = 4 to 6/d) were slaughtered on d 7, 14, 28 and 42 to 56 after parturition. In addition, a total of 11 postpartum cows that had begun cyclic activity were slaughtered on d 28, 42 or 56. Blood was collected at 10-min intervals for 6 h 1 d before slaughter for measurement of prolactin, cortisol and progesterone in serum. Numbers of medium (4.0 to 7.9 mm) follicles increased fourfold (P less than .05) between d 7 and 42 to 56 in anovulatory cows, whereas numbers of small (1.0 to 3.9 mm) and large (greater than or equal to 8.0 mm) follicles did not change (P greater than .10). Uterine involution was complete by d 28. In anovulatory cows, a higher (P less than .05) proportion of largest (but not second-largest) follicles was opposite the ovary containing the corpus albicans from pregnancy (CAP). In addition, 90% of these largest follicles opposite the CAP had concentrations of estradiol greater than progesterone. In cyclic cows, however, first ovulations occurred with equal frequency on either ovary. Concentrations of prolactin or cortisol in serum or duration of suckling were not associated with changes in uterine or ovarian measurements. In conclusion, growth and function of the largest (but not second-largest) follicle were reduced when located on the ovary containing the CAP.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Effect of dietary iodide on hypophyseal and thyroid hormone secretions in Holstein-Friesian heifers     

K Leung  E M Convey  G H Conner 《American journal of veterinary research》1980,41(9):1402-1407

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Xenogeneic Human Tyrosinase DNA Vaccination Induces Specific Immune Response in Dogs with Advanced Malignant Melanoma     

J.  Liao †  P. Gregor  F. Orlandi  D. M. Craft  C. Leung  A. N. Houghton  J. D. Wolchok  P. J. Bergman 《Veterinary and comparative oncology》2005,3(1):48-48

Introduction:  Xenogeneic melanosomal differentiation antigens, delivered in the form of a plasmid DNA vaccine, can overcome host immune ignorance/tolerance in preclinical animals to melanoma by: 1) generating humoral and cytotoxic T cell responses and 2) inducing protection from tumor challenge. Initial trials of human tyrosinase (huTyr) DNA vaccination of dogs with advanced malignant melanoma (Bergman et al , 2003) demonstrated safety and prolongation of survival with this therapeutic modality. We investigated antigen‐specific immunity in dogs receiving huTyr DNA vaccination.
Methods:  Three cohorts of three dogs each with advanced (WHO stage II‐IV) canine malignant melanoma (CMM) received four biweekly IM injections (dose levels 100, 500, or 1,500 ug, respectively/vaccination) of huTyr plasmid DNA via the Biojector2000 jet delivery device. Sera samples were taken before and after vaccination to detect specific antibody formation to huTyr by Enzyme‐Linked ImmunoSorbent Assays (ELISAs) and flow‐cytometry.
Results:  Three dogs have measurable, 2 to 4 fold huTyr‐specific antibody titers. Preliminary studies by flow‐cytometry have confirmed antibody response to huTyr by positive binding to endogenous human tyrosinase in SK‐Mel188 cells using post‐vaccinate serum.
Conclusions:  Xenogeneic (huTyr) DNA vaccination generates antigen‐specific humoral responses, which may partially explain the previously reported clinical efficacy and anti‐tumor responses. Ongoing studies include: 1) a comprehensive analysis by flow‐cytometry to detect huTyr‐specific antibodies and 2) a quantitative measure of potential cytotoxic T‐cell responses in these dogs by the DNA vaccination.  相似文献   

Rapid communication: complete nucleotide sequence of the chicken prolactin gene     

Au WL  Leung FC 《Journal of animal science》2002,80(5):1381

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Use of imiquimod 5% cream (Aldara™) in cats with multicentric squamous cell carcinoma in situ: 12 cases (2002–2005)*     

V. L. Gill  P. J. Bergman  K. E. Baer  D. Craft  C. Leung 《Veterinary and comparative oncology》2008,6(1):55-64

Multicentric squamous cell carcinoma in situ (MSCCIS) is a variant of squamous cell carcinoma in cats, commonly referred to as Bowen’s‐like disease. Imiquimod 5% cream (Aldara?) is a novel immune response modifier (IRM) that has been reported as a successful treatment for Bowen’s disease in humans. The purpose of this study was to describe clinical findings, treatment protocols and survival in cats with MSCCIS treated with imiquimod 5% cream and to examine the effects of imiquimod 5% cream in cats with MSCCIS. The expression of papillomavirus group‐specific antigen in the study population was also determined. From review of medical records, 12 cats were identified with a histologic diagnosis of MSCCIS and treatment with imiquimod 5% cream. Initial lesions responded to imiquimod 5% cream in all cats. Most cats (75%) developed new lesions. New lesions also responded to imiquimod 5% cream in all cats treated. Five cats (41%) had side effects suspected to be associated with the use of imiquimod 5% cream, including local erythema (25%), increased liver enzymes and neutropenia (8%), and partial anorexia and vomiting (8%). Kaplan–Meier median treatment duration and median survival time probabilities for cats in this study were 1189 days, respectively. A time to failure model was generated as many cats were censored from analysis well before the aforementioned projected median. This model resulted in a shorter median survival time of 243 days. No patient‐related, tumour‐related or treatment‐related prognostic variables were identified. No expression for papilloma group‐specific antigen was found. Imiquimod 5% cream appears to be well tolerated in the majority of cats, and further studies are warranted to further examine its usefulness in cats with this disease.  相似文献   

Comments to the predecessor of human SARS coronavirus in 2003-2004 epidemic     

Lam TT  Hon CC  Lam PY  Yip CW  Zeng F  Leung FC 《Veterinary microbiology》2008,126(4):390-393

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Veterinarian's responsibility in regard to a stray tattooed pet          下载免费PDF全文

Leung D 《The Canadian veterinary journal. La revue veterinaire canadienne》1997,38(11):679-680

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