Creation of a predictive equation to estimate fat-free mass and the ratio of fat-free mass to skeletal size using morphometry in lean working farm dogs     

YM Leung  BAS Hodgson 《New Zealand veterinary journal》2018,66(5):248-256

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Cloning and characterization of chicken growth hormone binding protein (cGHBP)     

Lau JS  Yip CW  Law KM  Leung FC 《Domestic animal endocrinology》2007,33(1):107-121

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Hong Kong SARS sequence     

Leung FC 《Science (New York, N.Y.)》2003,301(5631):309-310

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Expression Pattern of Apoptotic Genes in Vitrified‐Thawed Bovine Oocytes     

VM Anchamparuthy  RE Pearson  FC Gwazdauskas 《Reproduction in domestic animals》2010,45(5):e83-e90

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Apropos telemedicine.          下载免费PDF全文

D Leung 《The Canadian veterinary journal. La revue veterinaire canadienne》1999,40(5):318-320

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Does streptobacillary arthritis reduce house mouse population density,activity and damage to sorghum crops?     

Shahram Kaboodvandpour  Luke K.-P. Leung 《Journal of pest science》2007,80(3):167-174

An experiment to examine the effect of streptobacillary arthritis on mouse populations was conducted in maturing sorghum crops in mouse-proofed pens. There were three treatment pens and three control pens in which mice with and without clinical signs of streptobacillary arthritis were introduced, respectively. A constant mouse density (533 mice ha⁻¹) was introduced to the pens by releasing 12 mice in each pen. The sex ratio of introduced mice was also constant by releasing in each pen nine males and three females. Mean track index was lower in treatment pens than in control pens, indicating that streptobacillary arthritis reduced the activity of mice. However, this was not translated into a reduction in other population responses. Mean mouse density at harvest, percentage of female pregnant at harvest, yield loss caused by mice and a feeding index (oil card index) did not differ significantly between treatment and control groups. A plausible explanation for these findings was that mice with the clinical signs might be less active but were able to consume as much grains as mice without the clinical signs because food was in abundant supply.  相似文献   

Effect of Oil Overlay on Inhibition Potential of Roscovitine in Sheep Cumulus‐Oocyte Complexes          下载免费PDF全文

LF Crocomo  WC Marques Filho  CMV Ulian  NS Branchini  DT Silva  CL Ackermann  FC Landim‐Alvarenga  SD Bicudo 《Reproduction in domestic animals》2015,50(3):410-416

Inhibitors of cyclin‐dependent kinases, as roscovitine, have been used to prevent the spontaneous resumption of meiosis in vitro and to improve the oocyte developmental competence. In this study, the interference of oil overlay on the reversible arrest capacity of roscovitine in sheep oocytes as well as its effects on cumulus expansion was evaluated. For this, cumulus‐oocyte complexes (COCs) were cultured for 20 h in TCM 199 with 10% foetal bovine serum (Control) containing 75 μm roscovitine (Rosco). Subsequently, they were in vitro matured (IVM) for further 18 h in inhibitor‐free medium with LH and FSH. The culture was performed in Petri dishes under mineral oil (+) or in 96 well plates without oil overlay (?) at 38.5°C and 5% CO₂. At 20 and 38 h, the cumulus expansion and nuclear maturation were evaluated under stereomicroscope and by Hoechst 33342 staining, respectively. No group presented cumulus expansion at 20 h. After additional culture with gonadotrophins, a significant rate of COCs from both Control groups (+/?) exhibited total expansion while in both Rosco groups (+/?) the partial expansion prevailed. Among the oocytes treated with roscovitine, 65.2% were kept at GV in the absence of oil overlay while 40.6% of them reached MII under oil cover (p < 0.05). This meiotic arrest was reversible, and proper meiosis progression also occurred in the Control groups (+/?). So, the culture system without oil overlay improved the meiotic inhibition promoted by roscovitine without affecting the cumulus expansion rate or the subsequent meiosis progression.  相似文献   

Effect of Leptin on In Vivo Goat Embryo Production     

AM Batista  WA Gomes  CCD Carvalho  PLJ Monteiro Jr  FLM Silva  FC Almeida  PC Soares  GF Carneiro  MMP Guerra 《Reproduction in domestic animals》2014,49(3):476-480

The aim of this study was to evaluate the effect of leptin administration during superovulation on in vivo goat embryo production. Ten mature does were superovulated with 133 mg follicle‐stimulating hormone (FSH) i.m. in six descending doses at 12‐h intervals. The goats received 4.8 μg/kg human recombinant leptin s.c. (leptin group, n = 5) or phosphate‐buffered saline (PBS) (control group, n = 5) with the first and second FSH doses. The does were mated and subjected to embryo collection by transcervical technique 6 days later. The total number of cells per embryo and the number of cells with fragmented DNA were assessed in selected blastocysts by combining Hoechst 33342 and terminal dUTP nick‐end labelling (TUNEL) staining. Plasma concentrations of oestradiol (E₂) and progesterone (P₄) were determined by electrochemiluminescence from the day of FSH treatment, on the day of superovulatory oestrus and on the day before embryo collection. Compared with the control group, the does that received leptin had a higher number of transferable embryos (p < 0.005), fewer embryos classified as degenerated (p < 0.001) and fewer TUNEL‐positive cells/blastocyst (p < 0.001). The number of transferable embryos was positively correlated with E₂ concentrations on day of oestrus (r = 0.562; p < 0.01) and P₄ concentrations on the day of embryo collection (r = 0.912; p < 0.001). We concluded that in vivo leptin administration during FSH treatment improved embryo quality and affected ovarian steroidogenesis in superovulated goats.  相似文献   

Gonadotropin‐induced Puberty Does Not Impair Reproductive Performance of Gilts over Three Parities     

OHO Eckhardt  SMMK Martins  ME Pinese  FC Horta  AC Rosseto  MA Torres  AFC De Andrade  BBD Muro  CT Marino  PHM Rodrigues  AS Moretti 《Reproduction in domestic animals》2014,49(6):964-969

The aim of this study was to evaluate the reproductive performance of three parities of gilts treated or not treated with gonadotropin to induce puberty. Sixty gilts received 600 IU of equine chorionic gonadotropin (eCG) followed by 2.5 mg of porcine luteinizing hormone (LH) 72 h later. Fifty‐nine other gilts were exposed only to a mature boar for 15 min twice daily. Artificial insemination (AI) was performed at 0, 12 and 24 h after the detection of oestrus, and gestation was confirmed by ultrasound after 35 days. Sows were inseminated at the first post‐weaning oestrus. The total numbers of piglets born, piglets born alive, stillborn, mummified foetuses, as well as pregnancy and farrowing rates were evaluated for each of the three parities. Culling rates, farrowing intervals and weaning‐to‐oestrous intervals (WEI) were also analysed. Mean age at puberty and oestrous manifestation were not significantly different between treatments (p = 0.0639; 179.20 ± 17.52 compared with 173.96 ± 16.94, 91.66% compared with 94.92%) across the experimental period. However, females that underwent puberty induction showed modest increases both in the number of total pigs born and in the number of piglets born alive. In conclusion, puberty induction through exogenous gonadotropin administration in field conditions did not induce a more concentrated first oestrous manifestation, but trended to a modest increase in the number of pigs born alive in the first parity and a reduced culling rate during the first gestation.  相似文献   

Morphological changes in carp epithelial cells infected with Aeromonas hydrophila   总被引：1，自引：0，他引：1  

K Y Leung  T M Lim  T J Lam  Y M Sin 《Journal of fish diseases》1996,19(2):167-174

The in vitro interaction of Aeromonas hydrophila with epithehoma papillosum cells of carp (EPC) was studied. All the virulent and type strains invaded and multiplied inside EPC cells. Morphological changes were induced by the virulent and type strains during the processes of invasion and intracellular replication. Among the virulent group, strain PPD134/91 required the shortest time (30 min post-infection) to induce cytopathic effects in the EPC monolayers. The EPC cells infected with PPD 134/91 became retracted and condensed, lost their attachment abilities, and eventually disintegrated. Confocal microscopy revealed that tubular structures measuring 0.77 ± 0.19 μm appeared to connect the retracted EPC cells. The cytopathic effect was attributed to the growing and metabolically active bacteria. Avirulent strains such as L37 did not multiply nor induce cytopathic effects in the EPC monolayers.  相似文献