Metabolic changes induced by oral glucose tests in horses and their diagnostic use     

Julien Delarocque  Florian Frers  Karsten Feige  Korinna Huber  Klaus Jung  Tobias Warnken 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2021,35(1):597-605

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Milk traits of lactating cows submitted to feed restriction     

Alexandre Mossate Gabbi  Concepta Margareth McManus  Maira Balbinotti Zanela  Marcelo Tempel Stumpf  Rosângela Silveira Barbosa  Vilmar Fruscalso  André Thaler Neto  Fernando André Schmidt  Vivian Fischer 《Tropical animal health and production》2016,48(1):37-43

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Comparison of the complement fixation test and competitive ELISA for serodiagnosis of Anaplasma marginale infection in experimentally infected steers   总被引：1，自引：0，他引：1  

Coetzee JF  Schmidt PL  Apley MD  Reinbold JB  Kocan KM 《American journal of veterinary research》2007,68(8):872-878

OBJECTIVE: To compare sensitivity of a complement fixation (CF) test and competitive ELISA (cELISA) for detection of Anaplasma marginale in experimentally infected steers. ANIMALS: 40 crossbred (Angus-Simmental) steers. PROCEDURES: Steers were inoculated with 2.6 x 10(9) A marginale-infected erythrocytes (day 0). Blood samples were collected on days 9, 13, 20, 28, 34, 41, 61, 96, 126, and 156 days after inoculation. The percentage of parasitized erythrocytes (PPE) was determined by microscopic examination of stained blood films, and sera were evaluated with the CF test and cELISA by use of USDA-approved methods. Sensitivity and agreement (kappa statistic) between the 2 methods were determined. Persistent infections were confirmed by inoculation of blood obtained from infected steers into susceptible, splenectomized calves. RESULTS: 9 days after inoculation, sensitivity of the cELISA was 47.5%, whereas the CF test failed to identify seropositive steers. After day 13, sensitivity of the cELISA and CF test was 100% and 20%, respectively. During peak parasitemia (day 20), sensitivity of the cELISA and CF test was 100%. Thereafter, sensitivity of the CF test fluctuated between 7.5% and 37.5%, whereas sensitivity of the cELISA remained at 100%. Overall sensitivity of the cELISA and CF test was 94.8% and 26.5%, respectively (kappa statistic, 0.039). CONCLUSIONS AND CLINICAL RELEVANCE: The cELISA had superior sensitivity for serologic detection of A marginale.The CF test and cELISA each had a high percentage of false-negative results during the prepatent period. These findings are relevant for export certification and anaplasmosis prevention or eradication programs.  相似文献   

Shiga toxin-producing Escherichia coli and their bacteriophages as a model for the analysis of virulence and stress response of a food-borne pathogen     

Creuzburg K  Schmidt H 《Berliner und Münchener tier?rztliche Wochenschrift》2007,120(7-8):288-295

Enterohemorrhagic Escherichia coli (EHEC) are a subgroup of Shiga toxin-producing Escherichia coli (STEC) that are able to cause serious food-borne intestinal diseases which can be followed in 5 to 15% by extraintestinal sequelae such as the hemolytic-uremic syndrome (HUS). One of the major pathogenicity factors of EHEC is the production of one or more Shiga toxins (Stx), which act as inhibitors of protein biosynthesis and have profound effects on the signal transduction and immunological response in eukaryotic cells. The stx genes are located in the genome of heterogeneous, lambdoid, functional or cryptic bacteriophages and are expressed during the phage life cycle. Due to the linkage between the phage life cycle and stx expression, STEC and their bacteriophages are useful as a model for the analysis of stress response and virulence of this food-borne pathogen.  相似文献   

The porcine immune system--differences compared to man and mouse and possible consequences for infections by Salmonella serovars     

Scharek L  Tedin K 《Berliner und Münchener tier?rztliche Wochenschrift》2007,120(7-8):347-354

The porcine immune system differs in many aspects from that of humans and mice. Morphological differences in the lymphatic system (e.g. lymph nodes, Peyer's patches), and phenotypic differences in immune cells have been observed as well as functional differences in immune cell populations. Indeed, even the prenatal development of the embryonic piglet proceeds in a principally different way. However, it remains unclear to what extent these differences might contribute to the predisposition to and outcomes of bacterial infections, in particular those with zoonotic potential. In the following article we will review some of the peculiarities of the porcine immune system and consider possible implications for the course of infections in swine, with an emphasis on Salmonella serovars.  相似文献   

A unique intraorbital osseous structure in the large fruit-eating bat (Artibeus lituratus)     

Machado M  dos Santos Schmidt EM  Margarido TC  Montiani-Ferreira F 《Veterinary ophthalmology》2007,10(2):100-105

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Schiff-Sherrington syndrome in a 7-year-old zebra mare (Equus quagga)     

Florian Frers  Maren Hellige  Sandra Franke  Christina Puff  Uta Delling  Karsten Feige 《Equine Veterinary Education》2023,35(2):e98-e104

A 7-year-old plains zebra (Equus quagga) mare was presented to the Clinic for Horses, University of Veterinary Medicine Hannover, Foundation, Germany as an emergency. The owner reported that the mare was unable to stand after a suspected traumatic episode at the home yard. The mare was transported in lateral recumbency in a trailer. Clinical examination revealed tachycardia (up to 102 beats/min), tachypnoea (28 breaths/min), mild fever (38.7°C) and clinical dehydration with elastic skin and dry and red mucous membranes. Neurological examination showed paraplegia with complete loss of nociception of the mid-thoracic region and caudal to it. In contrast, examination of the thoracic limbs revealed marked extensor rigidity and hyperaesthesia resulting in an enhanced acute response to minimal touch of the neck and shoulder region. Lateral radiographs of the thoracic vertebral column led to the diagnosis of a complete fracture of the vertebral body of the 7th thoracic (T) vertebra with suspected traumatic compression of the spinal cord. Post-mortem examination confirmed the diagnosis. Gross pathology revealed a complete comminuted fracture of T7 with marked compression of the spinal cord. This is the first report describing the clinical, radiological and pathological findings of a fracture of a thoracic vertebra leading to a Schiff-Sherrington syndrome in a zebra (Equus quagga).  相似文献   

Contrasting edge and pasture matrix effects on ant diversity from fragmented landscapes across multiple spatial scales     

Lasmar  Chaim J.  Queiroz  Antônio C. M.  Rosa  Clarissa  Carvalho  Nathália S.  Schmidt  Fernando A.  Solar  Ricardo R. C.  Paolucci  Lucas N.  Cuissi  Rafael G.  Ribas  Carla R. 《Landscape Ecology》2021,36(9):2583-2597

Landscape Ecology - Harmful effects of habitat loss and fragmentation can be detected across multiple spatial scales, yet most studies that aim to characterize these effects take place at a single...  相似文献   

Land-use history has a stronger impact on soil microbial community composition than aboveground vegetation and soil properties     

Kamlesh Jangid  Mark A. Williams  Thomas M. Schmidt  William B. Whitman 《Soil biology & biochemistry》2011,43(10):2184-2193

The response of soil microbial communities following changes in land-use is governed by multiple factors. The objectives of this study were to investigate (i) whether soil microbial communities track the changes in aboveground vegetation during succession; and (ii) whether microbial communities return to their native state over time. Two successional gradients with different vegetation were studied at the W. K. Kellogg Biological Station, Michigan. The first gradient comprised a conventionally tilled cropland (CT), mid-succession forest (SF) abandoned from cultivation prior to 1951, and native deciduous forest (DF). The second gradient comprised the CT cropland, early-succession grassland (ES) restored in 1989, and long-term mowed grassland (MG). With succession, the total microbial PLFAs and soil microbial biomass C consistently increased in both gradients. While bacterial rRNA gene diversity remained unchanged, the abundance and composition of many bacterial phyla changed significantly. Moreover, microbial communities in the relatively pristine DF and MG soils were very similar despite major differences in soil properties and vegetation. After >50 years of succession, and despite different vegetation, microbial communities in SF were more similar to those in mature DF than in CT. In contrast, even after 17 years of succession, microbial communities in ES were more similar to CT than endpoint MG despite very different vegetation between CT and ES. This result suggested a lasting impact of cultivation history on the soil microbial community. With conversion of deciduous to conifer forest (CF), there was a significant change in multiple soil properties that correlated with changes in microbial biomass, rRNA gene diversity and community composition. In conclusion, history of land-use was a stronger determinant of the composition of microbial communities than vegetation and soil properties. Further, microbial communities in disturbed soils apparently return to their native state with time.  相似文献   

Stabilization mechanisms of organic matter in four temperate soils: Development and application of a conceptual model   总被引：2，自引：0，他引：2  

Margit von Lützow  Ingrid Kögel‐Knabner  Bernard Ludwig  Egbert Matzner  Heinz Flessa  Klemens Ekschmitt  Georg Guggenberger  Bernd Marschner  Karsten Kalbitz 《植物养料与土壤学杂志》2008,171(1):111-124

Based on recent findings in the literature, we developed a process‐oriented conceptual model that integrates all three process groups of organic matter (OM) stabilization in soils namely (1) selective preservation of recalcitrant compounds, (2) spatial inaccessibility to decomposer organisms, and (3) interactions of OM with minerals and metal ions. The model concept relates the diverse stabilization mechanisms to active, intermediate, and passive pools. The formation of the passive pool is regarded as hierarchical structured co‐action of various processes that are active under specific pedogenetic conditions. To evaluate the model, we used data of pool sizes and turnover times of soil OM fractions from horizons of two acid forest and two agricultural soils. Selective preservation of recalcitrant compounds is relevant in the active pool and particularly in soil horizons with high C contents. Biogenic aggregation preserves OM in the intermediate pool and is limited to topsoil horizons. Spatial inaccessibility due to the occlusion of OM in clay microstructures and due to the formation of hydrophobic surfaces stabilizes OM in the passive pool. If present, charcoal contributes to the passive pool mainly in topsoil horizons. The importance of organo‐mineral interactions for OM stabilization in the passive pool is well‐known and increases with soil depth. Hydrophobicity is particularly relevant in acid soils and in soils with considerable inputs of charcoal. We conclude that the stabilization potentials of soils are site‐ and horizon‐specific. Furthermore, management affects key stabilization mechanisms. Tillage increases the importance of organo‐mineral interactions for OM stabilization, and in Ap horizons with high microbial activity and C turnover, organo‐mineral interactions can contribute to OM stabilization in the intermediate pool. The application of our model showed that we need a better understanding of processes causing spatial inaccessibility of OM to decomposers in the passive pool.  相似文献