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Landscape Ecology - Harmful effects of habitat loss and fragmentation can be detected across multiple spatial scales, yet most studies that aim to characterize these effects take place at a single...  相似文献   
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Summary Four leaf enzymes malate dehydrogenase (MDH), 6-phosphogluconate dehydrogenase (6PGD), peroxidase (PX) and aspartate aminotransferase (AAT) of 17 walnut cultivars and two pollen enzymes malate dehydrogenase (MDH) and 6-phosphogluconate dehydrogenase (6PGD) of 15 walnut cultivars were analysed using horizontal starch gel electrophoresis. Walnut cultivars of different origin exhibited different numbers of electrophoretic bands and also different relative mobility. Different activity levels and phenotypic groups were detected in studied enzyme systems. Pollen enzymes revealed higher variability than enzymes extracted from the leaves. 15 walnut cultivars were classified into 10 malate dehydrogenase phenotypic groups and 14 6-phosphogluconate dehydrogenase phenotypic groups based on pollen analyses. 17 cultivars were classified into 9 peroxidase phenotypic groups and 7 6-phosphogluconate dehydrogenase phenotypic groups based on analyses of leaves. All of the 15 walnut cultivars could be identified and distinguished with electrophoretic analyses of MDH and 6PGD from the pollen while only 10 cultivars were distinguishable with analyses of 6PGD and PRX from the leaves. No variability useful for cultivar identification was observed in MDH and AAT from the leaves.  相似文献   
3.
In the frame of SAFENUT AGRI GEN RES Action, which was a European strategy for the recovery, characterization and conservation of genetic resources, the fatty acids and the tocopherol profiles of a set of 75 hazelnut accessions were analyzed. The aim of this study was to assess the genetic differences among the European germplasm, contributing to the definition of nut quality in traditional European areas of cultivation. Significant differences were found between accessions for oil amount and contents of most fatty acids. As expected, monounsaturated fatty acids made up the largest portion (mean 80.85 %) followed by polyunsaturated fatty acids (10.70 %). The saturated ones were the minor components and accounted for only 8.43 % of the total fatty acids. On the basis of Student’s test, significant differences between the 2 years of harvest were found for fatty acid content, except for linoleic acid, the ratio of polyunsaturated, α-tocopherol and the stability index. When the oil content was studied in cultivars from the same site of cultivation, the mean values of the genetic pools from central Italy (60.8 %), Slovenia (59.3 %) and Portugal (58.2 %) showed highest values than those of cultivars grown in Greece (56.8 %), Spain (55.9 %) and France (51.5 %). A chemometric approach based on principal component and clustering analyses was developed to identify the most interesting cultivars for breeding programs.  相似文献   
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Although the second largest chromosome of the genome, the X chromosome is usually excluded from genome-wide association studies (GWAS). Considering the presence and importance of genes on this chromosome that are involved in reproduction, the aim of this study was to evaluate the effect of its inclusion in GWAS on reproductive traits (scrotal circumference [SC], early pregnancy [P16] and age at first calving [AFC]) in a Nelore herd. Genotype data from 3,263 animals with the above-mentioned phenotypes were used. The results showed an increase in the variances explained by the autosomal markers for all traits when the X chromosome was not included. For SC, there was an increase of more than 10% for the windows on chromosomes 2 and 6. For P16, the effect was increased by almost 20% for windows on chromosome 5. The same pattern was found for AFC, with an increase of more than 10% for the most important windows. The results indicate that the noninclusion of the X chromosome can overestimate the effects of autosomes on SC, P16 and AFC not only because of the additive effect of the X chromosome itself but also because of its epistatic effect on autosomal genes.  相似文献   
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Journal of Plant Diseases and Protection - Phenolic compounds of three scab-resistant apple cultivars ‘Topaz’, ‘Goldstar’ and ‘Gold Rush’ and two susceptible...  相似文献   
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以日光温室栽培的蜜世界、鲁厚甜2号厚皮甜瓜品种为试材,通过分析不同摘顶植株叶片可溶性蛋白质(Pr)、叶绿素(Chl)、丙二醛(MDA)含量以及过氧化物酶(POD)活性变化,探讨了不同节位摘顶对厚皮甜瓜叶片衰老的影响。结果表明,在授粉后7d以内,两品种第18节、24节摘顶可使座瓜节位叶片维持较高的生理活性,第30节摘顶则明显偏低,表现为:前者叶片Pr、Chl含量明显高于后者,而MDA含量、POD活性则较低。授粉14d之后,两品种第24节摘顶延缓叶片衰老的效果明显优于第18节、30节摘顶。植株摘顶后,不同节位叶片生理指标的变化进一步证实,第24节摘顶可明显延缓叶片衰老。综合分析表明,24节左右为厚皮甜瓜合理摘顶节位,早熟品种可适当提高摘顶节位,而中晚熟品种应适当降低节位。  相似文献   
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This paper describes the effect of triploidy on growth and reproductive endocrinology in the months leading up to and including spawning in rainbow trout,Salmo gairdneri, and pink salmon,Oncorhynchus gorbuscha. Growth rates were the same for diploid and triploid rainbow trout, but triploid female pink salmon were smaller than maturing diploid females and diploid and triploid males of the same age. Triploid males of both species developed typical secondary sexual characteristics and had normal endocrine profiles, although their cycle appeared to be delayed by about one month. Triploid females remained silvery in appearance and showed no endocrine signs of maturation, even at the level of the pituitary. Thus, although triploids of both sexes are genetically sterile, only the females do not undergo physiological maturation.Reported in part at the Third International Symposium on Reproductive Physiology of Fish, St. John's, Newfoundland, August 2–7, 1987.  相似文献   
9.
The aim of this study was to assess the effect of the Chilean rodeo exercise on some blood constituents, classically used to assess welfare, during rest; training with and without steer; and before and after official competitions. During the training season, 13 horses were assessed at their farms of origins, and samples were taken at rest and after training with and without a steer; then during the competition season, 16 horses were assessed at four different times, one before and three after the competition. The blood constituents assessed were packed cell volume (PCV), total proteins, fibrinogen, cortisol, neutrophil:lymphocyte ratio, creatine phosphokinase, aspartate transaminase, glutathione peroxidase, and serum amyloid A. Analysis of variance for repeated measures was applied using the Statistix 8.0 software, and a significance level of P < .05 was applied. For horses assessed during exercise with and without a steer, significant differences were observed for PCV, which were significantly lower at rest than after both exercises, and total proteins, which showed a significant increase only after exercise with steer. In the case of official competitions, PCV was significantly higher immediately after rodeo, and the lowest value for cortisol was observed the evening after competition (P < .05); among the enzymes assessed, creatine phosphokinase plasma concentrations immediately after rodeo and the evening after competition were significantly higher than concentrations the next morning. The use of physiological indicators did not seem to be sufficient to assess the overall effect of rodeo on the welfare of these horses, and other types of indicators should be considered in future studies.  相似文献   
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