Influence of Two Extension Procedures on the Ubiquitous Microflora and the Biological Quality of Frozen Bovine Semen     

O Gérard  IJR Visser  HB Jansen 《Reproduction in domestic animals》1995,30(5):289-294

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Vitreous degeneration and associated ocular abnormalities in the dog     

Harathi Krishnan  Kathryn Diehl  Darko Stefanovski  Gustavo D. Aguirre 《Veterinary ophthalmology》2020,23(2):219-224

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Proteomic analysis of high protein soybean (Glycine max) accessions demonstrates the contribution of novel glycinin subunits     

Krishnan HB  Nelson RL 《Journal of agricultural and food chemistry》2011,59(6):2432-2439

Limited biochemical information is available on soybean accessions that have seed protein content greater than 45% of the seed dry weight. SDS-PAGE analysis of seed proteins from nine soybean accessions revealed significantly higher amount of seed storage proteins in these accessions when compared with that of soybean cultivar Williams 82. High-resolution two-dimensional gel electrophoretic analysis of seed proteins revealed significant differences among several seed storage protein components in these accessions. A total of 51 protein spots were identified using peptide mass fingerprinting (MALDI-TOF MS). The contribution of these proteins to the overall protein content of the accessions was quantified using Delta2D image analysis software. Results showed that among the majority of the nine accessions, the largest difference in higher protein quantity was within the seed 11S storage globulins. The high protein trait from PI407788A was successfully transferred to an experimental line, LG99-469, demonstrating that this trait was transferable and robust.  相似文献   

Colloidal nanocrystal shape and size control: the case of cobalt   总被引：1，自引：0，他引：1  

Puntes VF  Krishnan KM  Alivisatos AP 《Science (New York, N.Y.)》2001,291(5511):2115-2117

We show that a relatively simple approach for controlling the colloidal synthesis of anisotropic cadmium selenide semiconductor nanorods can be extended to the size-controlled preparation of magnetic cobalt nanorods as well as spherically shaped nanocrystals. This approach helps define a minimum feature set needed to separately control the sizes and shapes of nanocrystals. The resulting cobalt nanocrystals produce interesting two- and three-dimensional superstructures, including ribbons of nanorods.  相似文献   

Estimation of Coenzyme I Through the Uptake of Oxygen     

Krishnan PS 《Science (New York, N.Y.)》1947,105(2724):295

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PCR in a Rayleigh-Bénard convection cell     

Krishnan M  Ugaz VM  Burns MA 《Science (New York, N.Y.)》2002,298(5594):793

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An assessment of the genetic integrity of ex situ germplasm collections of three endangered species of Coffea from Madagascar: implications for the management of field germplasm collections     

Sarada Krishnan  Tom A. Ranker  Aaron P. Davis  Jean Jacques Rakotomalala 《Genetic Resources and Crop Evolution》2013,60(3):1021-1036

Madagascar has 59 species of Coffea, of which 42 are listed as Critically Endangered, Endangered, or Vulnerable by criteria of the Red List Category system of the World Conservation Union. In an attempt to assess the conservation value of ex situ collections of Malagasy coffee species, a study was undertaken using the field genebank collections maintained at the Kianjavato Coffee Research Station. Three species were selected for this purpose, C. kianjavatensis, C. montis-sacri, and C. vatovavyensis, and for comparative purposes extant, in situ populations of the same species were studied. Parentage analyses of ex situ propagated offspring of C. kianjavatensis and C. montis-sacri were performed to assess if crossing with other Coffea species maintained in the field genebank is compromising the genetic integrity of the collection. For these three species, higher genetic diversity was observed in the ex situ populations compared to the in situ populations, highlighting the importance of preserving the plants currently in ex situ collections. Parentage analyses of seed-propagated offspring of C. kianjavatensis and C. montis-sacri revealed that cross contamination with pollen from other Coffea species in the ex situ field genebank is occurring. These results have significant implications for the conservation management of wild Coffea species and for the management of ex situ genebanks.  相似文献   

The role of carbon dioxide during the onset of Antarctic glaciation     

Pagani M  Huber M  Liu Z  Bohaty SM  Henderiks J  Sijp W  Krishnan S  DeConto RM 《Science (New York, N.Y.)》2011,334(6060):1261-1264

Earth's modern climate, characterized by polar ice sheets and large equator-to-pole temperature gradients, is rooted in environmental changes that promoted Antarctic glaciation ~33.7 million years ago. Onset of Antarctic glaciation reflects a critical tipping point for Earth's climate and provides a framework for investigating the role of atmospheric carbon dioxide (CO(2)) during major climatic change. Previously published records of alkenone-based CO(2) from high- and low-latitude ocean localities suggested that CO(2) increased during glaciation, in contradiction to theory. Here, we further investigate alkenone records and demonstrate that Antarctic and subantarctic data overestimate atmospheric CO(2) levels, biasing long-term trends. Our results show that CO(2) declined before and during Antarctic glaciation and support a substantial CO(2) decrease as the primary agent forcing Antarctic glaciation, consistent with model-derived CO(2) thresholds.  相似文献   

Chemical characterization of high-yielding varieties of cashew (Anacardium occidentale)     

K. V. Nagaraja  V. M. Krishnan Nampoothiri 《Plant foods for human nutrition (Dordrecht, Netherlands)》1986,36(3):201-206

Cashew apples and kernels from sixteen high-yielding varieties were chemically characterized. Protein, starch, amino acids and sodium contents in cashew kernels did not vary significantly among the high-yielding varieties. Reducing sugar content in the kernel was negligible compared to total sugar. Ascorbic acid, amino acid, phenol and tannin contents in cashew apple showed a significant variation among high-yielding varieties. Non-reducing sugar content in cashew apples was negligible. Varieties M 6/1, Bla 256-1, M 10/4 and M 44/3 appeared to be better for apple juice extraction because of their low tannin content. No varietal difference was noticed with respect to qualitative composition of sugars, organic acids and phenols.CPCRI Contribution No. 459  相似文献   

Distal aortic aneurysm presumed to be secondary to an infected umbilical artery in a foal     

RM Archer  SJG Gordon  HB Carslake  MG Collett 《New Zealand veterinary journal》2013,61(1):65-68

Abstract

CASE HISTORY:?A 3-month-old female Warmblood foal was presented after displaying signs of colic with pyrexia for 5 days.

CLINICAL AND PATHOLOGICAL FINDINGS:?The foal continued to show signs of colic, frequently passed urine, and was pyrexic with an elevated white blood cell count. The umbilical stalk was thickened but there was no evidence of purulent material. Exploratory laparotomy revealed an enlarged left umbilical artery remnant tightly adhered to the bladder wall. The left umbilical artery continued to an aneurysm involving the distal aorta. The foal was subject to euthanasia and post-mortem examination confirmed a spherical aortic aneurysm, in the dorsal midline caudal to the kidneys that contained a large thrombus. Histopathological examination revealed inflammation and necrosis of the tunica intima and tunica media of the left umbilical artery with suppuration and bacterial colonies evident in the periarterial tissues.

DIAGNOSIS:?Infected aortic aneurysm presumably caused by an umbilical artery infection.

CLINICAL RELEVANCE:?A previously undetected umbilical infection appears to have resulted in an unusual delayed complication causing signs of colic in a foal. Veterinarians should be aware of this condition, and the possibility that it may be a cause of signs of colic in foals. Diagnosis based on ultrasonography should be possible, but may require sedation, visceral analgesia and careful examination.  相似文献