A framework for evaluation of on-farm mastitis diagnostics in Australia     

RN Zadoks  E Scholz  SM Rowe  JM Norris  HB Pooley  J House 《Australian veterinary journal》2023,101(4):142-152

Numerous culture-based diagnostics are available on the Australian and international markets for on-farm detection of bacterial pathogens in milk. Use of such diagnostics may provide an opportunity to improve the prudent use of antimicrobials in udder health management. Farms are low-resource settings in terms of diagnostic microbiology capacity. The World Health Organisation has identified criteria for the evaluation of diagnostic tests in low resource settings based on Accuracy, Sensitivity, Specificity, User-friendliness, being Rapid or Robust, Equipment-free and being Deliverable (ASSURED). Here, we review how those criteria can be interpreted in the context of microbiological diagnosis of mastitis pathogens, and how on-farm diagnostics that are currently available in Australia perform relative to ASSURED criteria. This evaluation identifies multiple trade-offs, both with regard to scientific criteria and with regards to convenience criteria. More importantly, the purpose of testing may differ between farms, and test performance should be evaluated relative to its intended use. The ability of on-farm mastitis diagnostics to inform mastitis treatment decision-making in a timely and cost-effective manner depends not just on test characteristics but also on farm-specific pathogen prevalence, and on the farm enterprise's priorities and the farm manager's potential courses of action. With most assay evaluations to date conducted in professional laboratories, there is a surprising dearth of information on how well any of the diagnostic tests perform on-farm and, indeed, of the on-farm decision-making processes that they aim to inform.  相似文献   

Effect of rinderpest vaccination of spermatogenesis in bulls     

K S Narasimhan  A R Krishnan  S A Quayam 《The Indian veterinary journal》1970,47(1):19-22

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Utilization of Weather Data in Predicting Bread Loaf Volume by Neural Network Method     

David Karki  Karl D. Glover  Krishna Bondalapati  Padmanaban G. Krishnan 《Cereal Chemistry》2016,93(5):471-477

The unique ability of wheat (Triticum aestivum L.) flour to produce cohesive dough has helped to make it the most widely used cereal crop for bread and other baked food products. Measurement of end‐use qualities, such as loaf volume, is ideally carried out through assessing loaves of bread; however, this is resource intensive. Predictive testing methods are more often utilized to identify wheat genotypes with potentially acceptable loaf volume, although more accurate predictive methods would be beneficial. Our objectives were to study the influence of weather observations on bread loaf volume and flour and dough quality data, to use a neural network (NN) model to predict loaf volume with select input data, and to compare the best multiple regression models identified with their NN counterparts. Weather data collected at 20 days after heading (DAH) showed the highest correlations with bread loaf volume when compared with prior intervals. A NN model containing maximum, minimum, and nighttime temperatures produced the highest coefficient of determination for predicting loaf volume. Our results showed that the NN model explained up to 20% more loaf volume variation than a similar regression model. Weather parameters representing conditions at 20 DAH played a significant role in loaf volume prediction.  相似文献   

Identification of glycinin and beta-conglycinin subunits that contribute to the increased protein content of high-protein soybean lines     

Krishnan HB  Natarajan SS  Mahmoud AA  Nelson RL 《Journal of agricultural and food chemistry》2007,55(5):1839-1845

Seed protein concentration of commercial soybean cultivars calculated on a dry weight basis ranges from approximately 37 to 42% depending on genotype and location. A concerted research effort is ongoing to further increase protein concentration. Several soybean plant introductions (PI) are known to contain greater than 50% protein. These PIs are exploited by breeders to incorporate the high-protein trait into commercial North American cultivars. Currently, limited information is available on the biochemical and genetic mechanisms that regulate high-proteins. In this study, we have carried out proteomic and molecular analysis of seed proteins of LG00-13260 and its parental high-protein lines PI 427138 and BARC-6. Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis revealed that the high-protein lines accumulated increased amounts of beta-conglycinin and glycinins, when compared with Williams 82. High-resolution two-dimensional electrophoresis utilizing pH 4-7 and pH 6-11 ampholytes enabled improved resolution of soybean seed proteins. A total of 38 protein spots, representing the different subunits of beta-conglycinin and glycinin, were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. High-protein was correlated with an increase in the accumulation of most of the subunits representing beta-conglycinin and glycinin. Comparisons of the amino acid profiles of high-protein soybean lines revealed that the concentration of sulfur amino acids, a reflection of protein quality, was not influenced by the protein concentration. Southern blot analysis showed the presence of genotypic variation at the DNA level between PI 427138 and BARC-6 for the genes encoding group1 glycinin, beta-conglycinin, Bowman-Birk inhibitor (BBI), and the Kunitz trypsin inhibitor (KTI). LG00-13260 inherited the allelic variants of the parental line PI 427138 for glycinin, beta-conglycinin, and KTI, while BBI was inherited from the parental line BARC-6. The results of our study indicate that high-seed protein concentration is attributed to greater accumulation of specific components of beta-conglycinin and glycinin subunits presumably mediated by preferential expression of these genes during seed development.  相似文献   

On energy absorption capacity,flexural and dynamic properties of flax/epoxy composite tubes     

Libo Yan  Nawawi Chouw  Krishnan Jayaraman 《Fibers and Polymers》2014,15(6):1270-1277

In this study, energy absorption capacity, flexural and dynamic properties of flax fibre reinforced epoxy polymer composite (FFRP) tubes are investigated. The energy absorption capacity of the tubes is investigated under uniaxial compression. Flexural behaviour of the tubes is studied under four-point bending and the dynamic properties (i.e., natural frequency and damping characteristics) are evaluated by impact hammer vibration testing of the tube specimens. The damping characteristics of the tubes are determined by using both a logarithmic decrement curve and the half-peak bandwidth method. The influence of tube laminate thickness and specimen size on the mechanical properties of FFRP tubes is determined. Compressive testing indicates that the FFRP tube provides a specific absorbed energy of 22 J/g, which is close to the conventional metal energy absorption materials, i.e. stainless steel and aluminium tubes. Flexural study shows that the FFRP tube exhibits a brittle failure as similar to that of the FFRP composites in a flat-coupon tension. The load carrying capacity and deflection of the tube increase with an increase in the tube thickness. Impact loading test concludes that an increase in tube thickness leads to a reduction in natural frequency and damping ratio of the tubes. The FFRP tubes have sizedependent dynamic properties, i.e. an increase in tube size increased the natural frequency but reduced the damping ratio of the specimens remarkably. However, all FFRP tubes have high damping ratios, thus reducing the effect of dynamic loading on the structural response. Therefore, this study suggests that FFRP tubes could be used in several structural applications, i.e. in automotive as energy absorbers and in civil infrastructure as poles.  相似文献   

Morphological and molecular characterization of Satputia (Luffa hermaphrodita Singh & Bhandari) revealed substantial diversity for use in breeding programme     

Bhutia  Nangsol D.  Sureja  Amish K.  Munshi  A. D.  Verma  Manjusha  Arya  Lalit  Krishnan  S. Gopala  Gurung  B. 《Genetic Resources and Crop Evolution》2022,69(7):2485-2500

Genetic Resources and Crop Evolution - Luffa hermaphrodita Singh & Bhandari, known as Satputia, a semi-wild taxon originating from L. graveolens is an underutilized vegetable. It has a...  相似文献   

Multiple Induced Spawnings of the Indian Catfish Heteropneustes fossilis (Bloch) within a Prolonged Spawning Season     

Deoraj  Alok  T. Krishnan    G. P. Talwar    Lalit C.  Garg 《Journal of the World Aquaculture Society》1998,29(2):252-258

Abstract Singhi, the Indian catfish Heteropneustes fossilis , attained gonadal maturity repeatedly in a single spawning cycle by photothermal treatment. After the first spawning, maintenance at 30 C and 14 h light/10 h dark for 3 wk induced recruitment of the next batch of mature oocytes for subsequent spawnings. The number of eggs released declined with each spawning. However, fertilizability of the eggs was maintained until the fifth spawning. Mature fish were induced to spawn by D-Lys⁶ salmon gonadotropin-releasing hormone agonist administration. The dose of salmon gonadotropin-releasing hormone agonist for the second and subsequent spawnings was determined to be 25-fold less compared to the dose administered for the first spawning. Fish released the maximum number of eggs upon induction when they were maintained continuously at 30 C and 14 h light/10 h dark for 6 wk. The result obtained in the present study points to a possibility of obtaining multiple spawnings from those species of farmed fish which spawn once a year.  相似文献   

Positional effect on protein and oil content and composition of soybeans     

Bennett JO  Krishnan AH  Wiebold WJ  Krishnan HB 《Journal of agricultural and food chemistry》2003,51(23):6882-6886

Soybean (Glycine max [L.] Merr.) protein and oil qualities, with respect to monogastric nutrition, have been linked to the relative abundance of specific protein subunits and fatty acids, respectively. An analysis of field-grown soybean seeds by near-infrared spectroscopy revealed significant differences in their protein and oil contents as a function of nodal position. Seed proteins from the plant apex were high in protein and low in oil content, while those from the basal region exhibited an opposite pattern of accumulation. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of total seed proteins revealed that the beta-subunit of beta-conglycinin content was 4-fold higher in seeds from the apical nodes than in seeds from basal nodes. The glycinin A3 polypeptide content gradually increased in successively lower nodes from the top of the plant. Its accumulation was drastically reduced when nitrogen was applied at specific growth stages. Exogenous nitrogen did not alter the pattern of beta-subunit accumulation, but accrual of the acidic and basic polypeptides of glycinin was diminished. The remaining seed storage protein components were not influenced by nodal position or nitrogen application. Gas chromatographic analysis of fatty acids indicated that only oleic (18:0) and linoleic (18:2) acids showed variability in accumulation at different nodes. Neither the abundance nor the distribution of the fatty acids was altered by nitrogen application.  相似文献   

Brain aluminum distribution in Alzheimer's disease and experimental neurofibrillary degeneration   总被引：31，自引：0，他引：31  

D R Crapper  S S Krishnan  A J Dalton 《Science (New York, N.Y.)》1973,180(85):511-513

Neurofibrillary degeneration is an important pathological finding in senile and presenile dementia of the Alzheimer type. Experimentally, aluminum induces neurofibrillary degeneration in neurons of higher mammals. Aluminum concentrations approaching those used experimentally have been found in some regions of the brains of patients with Alzheimer's disease.  相似文献   

A note on an embryonal nephroma met with in a fowl     

R Krishnan 《The Indian veterinary journal》1969,46(4):287-288

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