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51.
Cashew apples and kernels from sixteen high-yielding varieties were chemically characterized. Protein, starch, amino acids and sodium contents in cashew kernels did not vary significantly among the high-yielding varieties. Reducing sugar content in the kernel was negligible compared to total sugar. Ascorbic acid, amino acid, phenol and tannin contents in cashew apple showed a significant variation among high-yielding varieties. Non-reducing sugar content in cashew apples was negligible. Varieties M 6/1, Bla 256-1, M 10/4 and M 44/3 appeared to be better for apple juice extraction because of their low tannin content. No varietal difference was noticed with respect to qualitative composition of sugars, organic acids and phenols.CPCRI Contribution No. 459  相似文献   
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Abstract

CASE HISTORY:?A 3-month-old female Warmblood foal was presented after displaying signs of colic with pyrexia for 5 days.

CLINICAL AND PATHOLOGICAL FINDINGS:?The foal continued to show signs of colic, frequently passed urine, and was pyrexic with an elevated white blood cell count. The umbilical stalk was thickened but there was no evidence of purulent material. Exploratory laparotomy revealed an enlarged left umbilical artery remnant tightly adhered to the bladder wall. The left umbilical artery continued to an aneurysm involving the distal aorta. The foal was subject to euthanasia and post-mortem examination confirmed a spherical aortic aneurysm, in the dorsal midline caudal to the kidneys that contained a large thrombus. Histopathological examination revealed inflammation and necrosis of the tunica intima and tunica media of the left umbilical artery with suppuration and bacterial colonies evident in the periarterial tissues.

DIAGNOSIS:?Infected aortic aneurysm presumably caused by an umbilical artery infection.

CLINICAL RELEVANCE:?A previously undetected umbilical infection appears to have resulted in an unusual delayed complication causing signs of colic in a foal. Veterinarians should be aware of this condition, and the possibility that it may be a cause of signs of colic in foals. Diagnosis based on ultrasonography should be possible, but may require sedation, visceral analgesia and careful examination.  相似文献   
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The study was conducted to assess the effects of salinity on growth and biochemical composition of freshwater catfish, Clarias batrachus. A static nonrenewable acute toxicity bioassay test was conducted and LC50 of salinity for 96-h exposure to the fingerling (14.5 cm) was 12.52 ‰. Based on these results, two sublethal salinity levels, viz. 4 and 8 ‰ were selected to study the long-term effects of salinity on C. batrachus for a period of 90 days. From the study, it was found that growth and survival rate were less in saline water (4 and 8 ‰). Maximum growth and survival were recorded in freshwater (0 ‰ salinity) and subsequently at 4 and 8 ‰. To assess the biochemical alteration, few important biomarkers were estimated. At the end of 90 days rearing period, glucose level in the brain and blood of C. batrachus was found to decrease with salinity. The level of liver and muscle glycogen in the fish reared at 4 ‰ was lower than that of control. Ascorbic acid in all organs under study was found to decrease with increasing salinity, which was attributed to stress mitigation effect of vitamin C. Acetylcholine esterase (AchE) activity recorded a gradual decrease with increasing salinity. Metabolic enzymes, alkaline phosphatase (ALP) activity and adenosine triphosphosphtase (ATPase) activity also reduced both in liver and muscle tissues with increasing salinity. From the present investigation, it can be concluded that exposure to higher salinity significantly (P < 0.01) affects the growth and physiological response of Clarias batrachus.  相似文献   
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The high growth-stimulating effect of plant extract has urged the plant biotechnologists to use natural supplements in the culture media instead of synthetic phytohormones. We advocated the effect of sprouted sorghum extract(SSE) on emergence, in vitro acclimatization, and genetic fidelity in coleoptile derived callus of indica rice variety ADT36. The use of SSE with Murashige Skoog medium efficiently acclimatized the root and shoot apical systems. A higher mat and seminal roots(3.4 g biomass) with an efficient shoot primordium elongation were observed with an increase in the concentration of SSE. Seeds treated with SSE medium showed higher germination and earlier coleoptile maturation about 48 h compared to untreated seeds, and there was a higher expression of e EF-1α with an increase in coleoptile length. B5 medium was effective on inducing embryogenic and nodular callus from 3-day-old coleoptile with 3.0 mg/L 2,4-dichlorophenoxyacetic acid and further proliferated effectively with 0.8 mg/L kinetin with a fresh weight of 180 mg. Highly significant regeneration was observed with combination of 2.5 mg/L 6-benzylamino purine and 3.0 mg/L α-naphthaleneacetic acid. The metabolic and genetic profiles of in vitro and directly cultivated plants were the same, examined through Fourier-transform infrared spectroscopy, random amplified polymorphic DNA(RAPD), inter-simple sequence repeat(ISSR) and R-ISSR(combination of RAPD and ISSR) markers, respectively, and thus confirming the significant efficacy of the SSE incorporated medium. Disarmed T-DNA was transformed to coleoptile derived callus through Agrobacterium tumefaciens LBA4404 and confirmed by GUS assay. The T-DNA integration was confirmed by DNA blot analysis using DNA from transient GUS-expressed explants. Thus, SSE can be used as a natural and organic supplement for organogenesis and efficient acclimatizations of shoot and root apical meristems in regenerated plants.  相似文献   
57.
Wild species offer a potential reservoir of genetic variation for crop improvement. Besides the valuable genes for disease resistance that the wild species have provided for rice improvement, recent studies have shown that these wild species could also provide favorable alleles for the improvement of yield and yield-related traits. The present study reports yet another potential of wild relatives of rice, which involves the improvement of seed protein content. A significant increase in seed protein content was observed in an interspecific hybrid between Oryza sativa ssp. indica and the wild species Oryza nivara. The hybrid showed a protein content of 12.4%, which was 28 and 18.2% higher than those of the parents O. nivara and IR 64, respectively. The increase in protein content was dependent on the genetic background of the rice variety used in the hybridization. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of seed storage proteins demonstrated that a significant increase in prolamins and glutelins was mainly responsible for the elevated protein content of the hybrid. Amino acid analysis of seed proteins revealed that the hybrid had net gains of 19.5% in lysine and 19.4% in threonine over the O. nivara parent on a seed dry weight basis. Molecular analysis indicated that the increase in protein content of the hybrid was not a result of chromosomal rearrangements or transposable element activation, at least in the chromosomal regions containing seed storage protein genes. A preliminary genetic analysis of the F 2 segregating population showed that the inheritance of the increased protein content was polygenic in nature. The development of this interspecific hybrid offers a great potential for selecting new rice cultivars that combine the high yield and superior cooking quality of IR 64 with improved seed protein content.  相似文献   
58.
Tofu, a cheese-like food made by curdling soy milk, is a major dietary staple of Asian countries. Consumption of tofu and other soy products is steadily increasing in North America due to its well-known health benefits. Soybean A(5), A(4), and B(3) peptide null lines 'Enrei' and 'Raiden' are commonly utilized in breeding programs to develop high-quality tofu cultivars. To expand the genetic diversity it is desirable to identify and utilize other A(5), A(4), and B(3) null genotypes in the development of improved tofu cultivars that are adapted to North American conditions. In this study were screened diverse soybean accessions from the USDA Soybean Germplasm Collection to identify Gy4 mutants, the locus that controls A(5), A(4), and B(3) peptide production. Analysis of total seed proteins from 485 soybean lines by SDS-PAGE enabled the identification of 38 accessions that lacked the A(5), A(4), and B(3) peptides. These accessions showed marked differences in seed size and seed coat color and represented different maturity groups ranging from 0 to IX. To ascertain the molecular basis for the lack of A(5), A(4), and B(3) peptides in the newly identified Gy4 mutants, the nucleotide sequence of a portion of the Gy4 gene was determined from eight soybean accessions representing different maturity groups. These eight Gy4 mutants revealed a single point mutation that changed the translation initiation codon ATG to ATA, resulting in the A(5), A(4), and B(3) null phenotype. The newly identified Gy4 mutants from this study will enable plant breeders to expand the genetic diversity of North American food-quality soybeans and also aid in the development of hypoallergenic soybeans.  相似文献   
59.
This study presents the structural characteristics and regeneration potential of mangrove patches in the estuarine and coastal areas of Kerala, a tropical maritime state in India. Field surveys were carried out at 46 selected sites during August 2015 to May 2016. In each site, the vegetative structure and regeneration status were assessed using the quadrat method. Altogether 219 quadrates were laid out and a total of 13 true mangrove species, belonging to 5 families and 8 genera, were recorded. The total tree density and stand basal area of the study region was1678.08/ha and 20.33 m^2/ha respectively. The low basal areas indicate the reduced structural development in mangroves. Of the 13 tree species, Avicennia constitutes 56%of the total Important Value Index(IVI) and Avicennia officinalis represents 41% of the IVI in Kerala, followed by Avicennia marina(15%), Rhizophora mucronata(15%),Sonneratia alba(8%) Rhizophora apiculata(7%) and Excoecaria agallocha(7%). The diameter at breast height(DBH) in the study area revealed that 47% of the tree species came under the 1–10 cm DBH class. Total sapling and seedling density in Kerala was 2238.35 and 3232.42 individuals/ha respectively. Density of young plants(seedlings ? saplings) was only 31% greater of tree density and varied from 3–63%, which indicates poor regeneration potential. The Maturity index value(MIV) and complexity index(Ic) value of mangroves were 18.30 and 109.81 respectively. However, the low Ic value(\ 10) observed in seven out of ten coastal districts indicated poor structural development of mangroves in Kerala. Therefore, locationspecific conservation and management measures, guided by the knowledge on spatial distribution and habitat requirements of mangrove varieties should be taken to preserve the mangrove diversity of Kerala.  相似文献   
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